Experiment: PYE with Nalidixic 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00764 and CCNA_00765 are separated by 255 nucleotides CCNA_00765 and CCNA_00766 are separated by 158 nucleotides CCNA_00766 and CCNA_00767 are separated by 37 nucleotides CCNA_00767 and CCNA_03936 are separated by 284 nucleotides CCNA_03936 and CCNA_00768 overlap by 65 nucleotides CCNA_00768 and CCNA_00769 overlap by 4 nucleotides
CCNA_00764: CCNA_00764 - electron transfer flavoprotein beta subunit, at 822,151 to 822,897
_00764
CCNA_00765: CCNA_00765 - hypothetical protein, at 823,153 to 823,443
_00765
CCNA_00766: CCNA_00766 - cobalamin adenosyltransferase family protein, at 823,602 to 824,180
_00766
CCNA_00767: CCNA_00767 - hypothetical protein, at 824,218 to 824,421
_00767
CCNA_03936: CCNA_03936 - hypothetical protein, at 824,706 to 824,840
_03936
CCNA_00768: CCNA_00768 - hypothetical protein, at 824,776 to 825,000
_00768
CCNA_00769: CCNA_00769 - TetR family transcriptional regulator, at 824,997 to 825,644
_00769
Position (kb) 823
824
825 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 822.912 kb on - strand at 822.917 kb on - strand at 822.919 kb on - strand at 822.995 kb on - strand at 823.047 kb on + strand at 823.048 kb on + strand at 823.056 kb on - strand at 823.071 kb on + strand at 823.071 kb on + strand at 823.089 kb on + strand at 823.107 kb on + strand at 823.107 kb on + strand at 823.107 kb on + strand at 823.114 kb on + strand at 823.115 kb on - strand at 823.122 kb on - strand at 823.144 kb on + strand at 823.145 kb on + strand at 823.158 kb on - strand at 823.163 kb on + strand at 823.184 kb on - strand, within CCNA_00765 at 823.195 kb on - strand, within CCNA_00765 at 823.207 kb on - strand, within CCNA_00765 at 823.214 kb on - strand, within CCNA_00765 at 823.229 kb on + strand, within CCNA_00765 at 823.279 kb on + strand, within CCNA_00765 at 823.353 kb on + strand, within CCNA_00765 at 823.353 kb on + strand, within CCNA_00765 at 823.361 kb on - strand, within CCNA_00765 at 823.361 kb on - strand, within CCNA_00765 at 823.361 kb on - strand, within CCNA_00765 at 823.361 kb on - strand, within CCNA_00765 at 823.368 kb on + strand, within CCNA_00765 at 823.368 kb on + strand, within CCNA_00765 at 823.395 kb on - strand, within CCNA_00765 at 823.449 kb on + strand at 823.457 kb on - strand at 823.458 kb on + strand at 823.477 kb on + strand at 823.505 kb on + strand at 823.505 kb on + strand at 823.505 kb on - strand at 823.513 kb on - strand at 823.513 kb on - strand at 823.513 kb on - strand at 823.516 kb on + strand at 823.516 kb on + strand at 823.516 kb on + strand at 823.516 kb on + strand at 823.516 kb on + strand at 823.516 kb on + strand at 823.516 kb on + strand at 823.516 kb on + strand at 823.516 kb on + strand at 823.516 kb on + strand at 823.524 kb on - strand at 823.524 kb on - strand at 823.524 kb on - strand at 823.524 kb on - strand at 823.536 kb on + strand at 823.536 kb on + strand at 823.537 kb on - strand at 823.539 kb on - strand at 823.560 kb on + strand at 823.574 kb on + strand at 823.574 kb on + strand at 823.574 kb on + strand at 823.574 kb on + strand at 823.574 kb on + strand at 823.574 kb on + strand at 823.574 kb on + strand at 823.575 kb on + strand at 823.582 kb on - strand at 823.582 kb on - strand at 823.582 kb on - strand at 823.582 kb on - strand at 823.582 kb on - strand at 823.582 kb on - strand at 823.582 kb on - strand at 823.582 kb on - strand at 823.582 kb on - strand at 823.582 kb on - strand at 823.582 kb on - strand at 823.582 kb on - strand at 823.582 kb on - strand at 823.583 kb on - strand at 823.597 kb on + strand at 823.597 kb on + strand at 823.618 kb on + strand at 823.618 kb on + strand at 823.743 kb on - strand, within CCNA_00766 at 823.743 kb on - strand, within CCNA_00766 at 823.746 kb on + strand, within CCNA_00766 at 823.754 kb on - strand, within CCNA_00766 at 823.754 kb on - strand, within CCNA_00766 at 823.770 kb on + strand, within CCNA_00766 at 823.778 kb on - strand, within CCNA_00766 at 823.790 kb on + strand, within CCNA_00766 at 823.791 kb on + strand, within CCNA_00766 at 823.791 kb on + strand, within CCNA_00766 at 823.804 kb on - strand, within CCNA_00766 at 823.824 kb on - strand, within CCNA_00766 at 823.831 kb on + strand, within CCNA_00766 at 823.836 kb on + strand, within CCNA_00766 at 823.841 kb on + strand, within CCNA_00766 at 823.844 kb on - strand, within CCNA_00766 at 823.863 kb on - strand, within CCNA_00766 at 823.904 kb on - strand, within CCNA_00766 at 823.935 kb on - strand, within CCNA_00766 at 823.935 kb on - strand, within CCNA_00766 at 823.940 kb on + strand, within CCNA_00766 at 823.945 kb on + strand, within CCNA_00766 at 823.948 kb on - strand, within CCNA_00766 at 823.948 kb on - strand, within CCNA_00766 at 823.948 kb on - strand, within CCNA_00766 at 823.950 kb on + strand, within CCNA_00766 at 823.953 kb on - strand, within CCNA_00766 at 823.953 kb on - strand, within CCNA_00766 at 823.953 kb on - strand, within CCNA_00766 at 823.967 kb on + strand, within CCNA_00766 at 823.967 kb on + strand, within CCNA_00766 at 823.967 kb on + strand, within CCNA_00766 at 823.967 kb on + strand, within CCNA_00766 at 823.975 kb on - strand, within CCNA_00766 at 823.975 kb on - strand, within CCNA_00766 at 824.009 kb on + strand, within CCNA_00766 at 824.028 kb on + strand, within CCNA_00766 at 824.037 kb on + strand, within CCNA_00766 at 824.053 kb on + strand, within CCNA_00766 at 824.053 kb on + strand, within CCNA_00766 at 824.053 kb on + strand, within CCNA_00766 at 824.053 kb on + strand, within CCNA_00766 at 824.091 kb on + strand, within CCNA_00766 at 824.100 kb on + strand, within CCNA_00766 at 824.111 kb on - strand, within CCNA_00766 at 824.163 kb on - strand at 824.223 kb on - strand at 824.267 kb on + strand, within CCNA_00767 at 824.309 kb on + strand, within CCNA_00767 at 824.309 kb on + strand, within CCNA_00767 at 824.317 kb on - strand, within CCNA_00767 at 824.317 kb on - strand, within CCNA_00767 at 824.368 kb on + strand, within CCNA_00767 at 824.376 kb on - strand, within CCNA_00767 at 824.410 kb on + strand at 824.412 kb on + strand at 824.412 kb on + strand at 824.420 kb on - strand at 824.420 kb on - strand at 824.420 kb on - strand at 824.424 kb on + strand at 824.429 kb on + strand at 824.429 kb on + strand at 824.429 kb on + strand at 824.429 kb on + strand at 824.437 kb on - strand at 824.437 kb on - strand at 824.442 kb on - strand at 824.464 kb on - strand at 824.518 kb on + strand at 824.578 kb on + strand at 824.607 kb on + strand at 824.622 kb on - strand at 824.732 kb on + strand, within CCNA_03936 at 824.735 kb on - strand, within CCNA_03936 at 824.764 kb on + strand, within CCNA_03936 at 824.764 kb on + strand, within CCNA_03936 at 824.772 kb on + strand, within CCNA_03936 at 824.780 kb on - strand at 824.808 kb on + strand at 824.808 kb on + strand at 824.816 kb on - strand at 824.816 kb on - strand at 824.853 kb on + strand, within CCNA_00768 at 824.873 kb on - strand, within CCNA_00768 at 824.904 kb on - strand, within CCNA_00768 at 824.925 kb on - strand, within CCNA_00768 at 824.925 kb on - strand, within CCNA_00768 at 824.944 kb on + strand, within CCNA_00768 at 824.950 kb on - strand, within CCNA_00768 at 824.950 kb on - strand, within CCNA_00768 at 824.980 kb on + strand at 825.001 kb on + strand at 825.016 kb on + strand at 825.016 kb on + strand at 825.024 kb on - strand at 825.030 kb on + strand at 825.048 kb on - strand at 825.065 kb on + strand, within CCNA_00769 at 825.072 kb on - strand, within CCNA_00769 at 825.127 kb on + strand, within CCNA_00769 at 825.127 kb on + strand, within CCNA_00769 at 825.135 kb on - strand, within CCNA_00769
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Nalidixic 0.06 mg/ml remove 822,912 - +1.9 822,917 - -1.5 822,919 - +3.5 822,995 - -0.0 823,047 + +2.7 823,048 + -0.7 823,056 - -1.7 823,071 + +0.4 823,071 + +2.1 823,089 + +0.3 823,107 + +0.7 823,107 + +0.5 823,107 + -0.1 823,114 + -0.6 823,115 - -0.1 823,122 - -2.0 823,144 + -1.2 823,145 + -0.3 823,158 - +0.7 823,163 + +0.5 823,184 - CCNA_00765 0.11 +0.5 823,195 - CCNA_00765 0.14 +0.9 823,207 - CCNA_00765 0.19 +0.6 823,214 - CCNA_00765 0.21 -1.4 823,229 + CCNA_00765 0.26 +0.9 823,279 + CCNA_00765 0.43 -0.9 823,353 + CCNA_00765 0.69 -2.3 823,353 + CCNA_00765 0.69 -0.1 823,361 - CCNA_00765 0.71 +0.4 823,361 - CCNA_00765 0.71 -0.8 823,361 - CCNA_00765 0.71 -1.2 823,361 - CCNA_00765 0.71 +1.1 823,368 + CCNA_00765 0.74 +1.6 823,368 + CCNA_00765 0.74 +1.0 823,395 - CCNA_00765 0.83 -1.3 823,449 + +0.9 823,457 - +3.8 823,458 + -2.9 823,477 + -2.4 823,505 + -0.8 823,505 + -4.0 823,505 - -0.5 823,513 - +3.4 823,513 - -0.8 823,513 - -0.8 823,516 + -2.3 823,516 + +1.7 823,516 + +0.1 823,516 + +2.1 823,516 + -0.8 823,516 + -0.1 823,516 + +0.2 823,516 + -2.5 823,516 + -0.4 823,516 + +0.1 823,524 - -2.5 823,524 - -0.8 823,524 - -0.0 823,524 - +1.0 823,536 + -0.7 823,536 + +1.7 823,537 - +0.1 823,539 - -3.9 823,560 + -0.8 823,574 + -0.7 823,574 + -0.4 823,574 + -1.1 823,574 + +0.6 823,574 + +2.1 823,574 + -0.8 823,574 + -0.3 823,575 + +1.8 823,582 - +1.5 823,582 - -0.5 823,582 - -3.3 823,582 - -1.7 823,582 - +0.0 823,582 - -1.0 823,582 - +1.0 823,582 - +1.2 823,582 - -2.8 823,582 - -2.3 823,582 - -0.3 823,582 - -2.3 823,582 - -0.1 823,583 - +4.4 823,597 + -2.1 823,597 + -1.1 823,618 + +0.4 823,618 + +2.9 823,743 - CCNA_00766 0.24 +5.1 823,743 - CCNA_00766 0.24 +4.2 823,746 + CCNA_00766 0.25 +1.4 823,754 - CCNA_00766 0.26 +3.2 823,754 - CCNA_00766 0.26 +4.5 823,770 + CCNA_00766 0.29 +4.0 823,778 - CCNA_00766 0.30 +5.2 823,790 + CCNA_00766 0.32 +4.2 823,791 + CCNA_00766 0.33 +4.6 823,791 + CCNA_00766 0.33 +3.7 823,804 - CCNA_00766 0.35 +3.4 823,824 - CCNA_00766 0.38 +4.4 823,831 + CCNA_00766 0.40 +5.3 823,836 + CCNA_00766 0.40 +0.8 823,841 + CCNA_00766 0.41 +4.3 823,844 - CCNA_00766 0.42 +4.3 823,863 - CCNA_00766 0.45 +4.2 823,904 - CCNA_00766 0.52 +3.6 823,935 - CCNA_00766 0.58 +4.3 823,935 - CCNA_00766 0.58 +3.9 823,940 + CCNA_00766 0.58 +2.6 823,945 + CCNA_00766 0.59 +3.2 823,948 - CCNA_00766 0.60 +4.7 823,948 - CCNA_00766 0.60 +2.4 823,948 - CCNA_00766 0.60 +4.9 823,950 + CCNA_00766 0.60 +2.8 823,953 - CCNA_00766 0.61 +3.8 823,953 - CCNA_00766 0.61 +4.5 823,953 - CCNA_00766 0.61 +4.7 823,967 + CCNA_00766 0.63 +3.5 823,967 + CCNA_00766 0.63 +1.1 823,967 + CCNA_00766 0.63 +1.0 823,967 + CCNA_00766 0.63 +3.3 823,975 - CCNA_00766 0.64 +4.8 823,975 - CCNA_00766 0.64 +0.7 824,009 + CCNA_00766 0.70 +1.8 824,028 + CCNA_00766 0.74 +0.5 824,037 + CCNA_00766 0.75 +0.8 824,053 + CCNA_00766 0.78 +1.8 824,053 + CCNA_00766 0.78 +2.8 824,053 + CCNA_00766 0.78 +3.9 824,053 + CCNA_00766 0.78 +3.9 824,091 + CCNA_00766 0.84 +3.9 824,100 + CCNA_00766 0.86 +1.6 824,111 - CCNA_00766 0.88 +2.5 824,163 - -1.4 824,223 - +0.1 824,267 + CCNA_00767 0.24 -1.2 824,309 + CCNA_00767 0.45 -0.1 824,309 + CCNA_00767 0.45 +0.4 824,317 - CCNA_00767 0.49 +0.7 824,317 - CCNA_00767 0.49 -1.7 824,368 + CCNA_00767 0.74 +1.2 824,376 - CCNA_00767 0.77 -2.1 824,410 + -0.3 824,412 + +2.2 824,412 + +0.6 824,420 - -0.8 824,420 - +0.4 824,420 - -0.0 824,424 + +0.5 824,429 + +0.8 824,429 + +0.1 824,429 + +0.6 824,429 + +0.9 824,437 - -0.6 824,437 - +1.2 824,442 - +1.9 824,464 - +0.3 824,518 + +2.2 824,578 + -0.3 824,607 + -1.4 824,622 - -1.3 824,732 + CCNA_03936 0.19 +2.3 824,735 - CCNA_03936 0.21 -0.7 824,764 + CCNA_03936 0.43 +0.2 824,764 + CCNA_03936 0.43 +1.6 824,772 + CCNA_03936 0.49 +1.9 824,780 - -0.5 824,808 + +2.0 824,808 + +1.9 824,816 - +0.4 824,816 - -1.4 824,853 + CCNA_00768 0.34 +1.3 824,873 - CCNA_00768 0.43 +1.4 824,904 - CCNA_00768 0.57 +2.7 824,925 - CCNA_00768 0.66 -1.2 824,925 - CCNA_00768 0.66 -0.5 824,944 + CCNA_00768 0.75 +1.1 824,950 - CCNA_00768 0.77 -0.4 824,950 - CCNA_00768 0.77 +3.2 824,980 + +2.0 825,001 + +0.3 825,016 + +3.9 825,016 + +2.8 825,024 - +1.3 825,030 + +4.1 825,048 - +0.7 825,065 + CCNA_00769 0.10 +3.5 825,072 - CCNA_00769 0.12 +0.4 825,127 + CCNA_00769 0.20 +3.7 825,127 + CCNA_00769 0.20 +3.8 825,135 - CCNA_00769 0.21 -0.3
Or see this region's nucleotide sequence