Experiment: PYE with Nalidixic 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00272 and CCNA_00273 are separated by 8 nucleotides CCNA_00273 and CCNA_00274 are separated by 340 nucleotides CCNA_00274 and CCNA_00275 are separated by 83 nucleotides
CCNA_00272: CCNA_00272 - RmuC family protein, at 285,476 to 286,765
_00272
CCNA_00273: CCNA_00273 - peptide deformylase, at 286,774 to 287,295
_00273
CCNA_00274: CCNA_00274 - hypothetical protein, at 287,636 to 287,998
_00274
CCNA_00275: CCNA_00275 - transposase, DDE family, at 288,082 to 289,527
_00275
Position (kb) 287
288 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 286.641 kb on - strand at 286.705 kb on - strand at 286.706 kb on + strand at 286.716 kb on + strand at 286.734 kb on + strand at 286.770 kb on + strand at 286.778 kb on - strand at 287.268 kb on + strand at 287.290 kb on + strand at 287.290 kb on + strand at 287.290 kb on + strand at 287.290 kb on + strand at 287.290 kb on + strand at 287.290 kb on + strand at 287.290 kb on + strand at 287.298 kb on - strand at 287.298 kb on - strand at 287.299 kb on - strand at 287.355 kb on + strand at 287.363 kb on - strand at 287.381 kb on + strand at 287.381 kb on + strand at 287.389 kb on - strand at 287.416 kb on - strand at 287.441 kb on - strand at 287.474 kb on - strand at 287.516 kb on + strand at 287.526 kb on + strand at 287.526 kb on + strand at 287.531 kb on + strand at 287.531 kb on + strand at 287.536 kb on + strand at 287.536 kb on + strand at 287.536 kb on + strand at 287.536 kb on + strand at 287.536 kb on + strand at 287.539 kb on - strand at 287.544 kb on - strand at 287.544 kb on - strand at 287.544 kb on - strand at 287.544 kb on - strand at 287.544 kb on - strand at 287.544 kb on - strand at 287.544 kb on - strand at 287.586 kb on - strand at 287.631 kb on + strand at 287.631 kb on + strand at 287.666 kb on + strand at 287.691 kb on - strand, within CCNA_00274 at 287.738 kb on + strand, within CCNA_00274 at 287.748 kb on + strand, within CCNA_00274 at 287.791 kb on + strand, within CCNA_00274 at 287.808 kb on - strand, within CCNA_00274 at 287.882 kb on + strand, within CCNA_00274 at 287.902 kb on + strand, within CCNA_00274 at 287.910 kb on - strand, within CCNA_00274 at 287.987 kb on + strand at 287.987 kb on + strand at 287.990 kb on - strand at 288.007 kb on - strand at 288.017 kb on - strand at 288.027 kb on - strand at 288.027 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Nalidixic 0.06 mg/ml remove 286,641 - -1.1 286,705 - -1.5 286,706 + +1.2 286,716 + +0.2 286,734 + +1.7 286,770 + -0.7 286,778 - -1.9 287,268 + +2.1 287,290 + -0.2 287,290 + +1.4 287,290 + +1.3 287,290 + -1.2 287,290 + -0.7 287,290 + +2.4 287,290 + +2.0 287,298 - -0.3 287,298 - -0.4 287,299 - -0.4 287,355 + +0.4 287,363 - +0.2 287,381 + -3.5 287,381 + +0.3 287,389 - +1.3 287,416 - -2.1 287,441 - -2.4 287,474 - +0.9 287,516 + -1.9 287,526 + +0.9 287,526 + +1.9 287,531 + +2.1 287,531 + -1.0 287,536 + +0.2 287,536 + +1.2 287,536 + +0.2 287,536 + -0.2 287,536 + -1.0 287,539 - -2.3 287,544 - -0.9 287,544 - -0.0 287,544 - +0.7 287,544 - -4.1 287,544 - -1.3 287,544 - +1.8 287,544 - -3.5 287,586 - -0.2 287,631 + -1.8 287,631 + +1.9 287,666 + -1.0 287,691 - CCNA_00274 0.15 -0.5 287,738 + CCNA_00274 0.28 +0.0 287,748 + CCNA_00274 0.31 -0.5 287,791 + CCNA_00274 0.43 +0.7 287,808 - CCNA_00274 0.47 -1.7 287,882 + CCNA_00274 0.68 -1.4 287,902 + CCNA_00274 0.73 -0.4 287,910 - CCNA_00274 0.75 -1.5 287,987 + +3.8 287,987 + +1.8 287,990 - -0.4 288,007 - +0.7 288,017 - +2.0 288,027 - -0.5 288,027 - +2.2
Or see this region's nucleotide sequence