Experiment: PYE with Nalidixic 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00224 and CCNA_00225 are separated by 330 nucleotides CCNA_00225 and CCNA_00226 are separated by 100 nucleotides CCNA_00226 and CCNA_00227 are separated by 57 nucleotides CCNA_00227 and CCNA_00228 overlap by 1 nucleotides
CCNA_00224: CCNA_00224 - TonB-dependent receptor protein, at 240,648 to 242,693
_00224
CCNA_00225: CCNA_00225 - hypothetical protein, at 243,024 to 243,056
_00225
CCNA_00226: CCNA_00226 - hypothetical protein, at 243,157 to 243,432
_00226
CCNA_00227: CCNA_00227 - methyltransferase, at 243,490 to 244,056
_00227
CCNA_00228: CCNA_00228 - putative membrane spanning protein, at 244,056 to 244,670
_00228
Position (kb) 243
244 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 242.164 kb on + strand, within CCNA_00224 at 242.164 kb on + strand, within CCNA_00224 at 242.164 kb on - strand, within CCNA_00224 at 242.170 kb on - strand, within CCNA_00224 at 242.172 kb on - strand, within CCNA_00224 at 242.172 kb on - strand, within CCNA_00224 at 242.188 kb on + strand, within CCNA_00224 at 242.196 kb on - strand, within CCNA_00224 at 242.209 kb on + strand, within CCNA_00224 at 242.224 kb on - strand, within CCNA_00224 at 242.224 kb on - strand, within CCNA_00224 at 242.250 kb on + strand, within CCNA_00224 at 242.264 kb on + strand, within CCNA_00224 at 242.300 kb on + strand, within CCNA_00224 at 242.305 kb on + strand, within CCNA_00224 at 242.338 kb on + strand, within CCNA_00224 at 242.343 kb on + strand, within CCNA_00224 at 242.373 kb on - strand, within CCNA_00224 at 242.428 kb on - strand, within CCNA_00224 at 242.441 kb on - strand, within CCNA_00224 at 242.441 kb on - strand, within CCNA_00224 at 242.481 kb on + strand, within CCNA_00224 at 242.489 kb on - strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.535 kb on - strand at 242.535 kb on - strand at 242.535 kb on - strand at 242.535 kb on - strand at 242.535 kb on - strand at 242.565 kb on - strand at 242.573 kb on - strand at 242.587 kb on + strand at 242.587 kb on + strand at 242.595 kb on - strand at 242.600 kb on - strand at 242.643 kb on + strand at 242.656 kb on - strand at 242.675 kb on - strand at 242.683 kb on - strand at 242.704 kb on + strand at 242.715 kb on + strand at 242.716 kb on - strand at 242.718 kb on - strand at 242.723 kb on - strand at 242.723 kb on - strand at 242.892 kb on + strand at 242.892 kb on + strand at 242.892 kb on + strand at 242.892 kb on + strand at 242.892 kb on + strand at 242.892 kb on + strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.904 kb on + strand at 242.932 kb on + strand at 242.987 kb on + strand at 242.995 kb on - strand at 243.010 kb on + strand at 243.018 kb on - strand at 243.020 kb on + strand at 243.028 kb on - strand, within CCNA_00225 at 243.045 kb on + strand, within CCNA_00225 at 243.050 kb on + strand, within CCNA_00225 at 243.050 kb on + strand, within CCNA_00225 at 243.068 kb on - strand at 243.083 kb on + strand at 243.091 kb on - strand at 243.154 kb on + strand at 243.162 kb on - strand at 243.162 kb on - strand at 243.162 kb on - strand at 243.162 kb on - strand at 243.164 kb on + strand at 243.164 kb on + strand at 243.164 kb on + strand at 243.198 kb on + strand, within CCNA_00226 at 243.198 kb on + strand, within CCNA_00226 at 243.202 kb on - strand, within CCNA_00226 at 243.205 kb on + strand, within CCNA_00226 at 243.206 kb on - strand, within CCNA_00226 at 243.213 kb on + strand, within CCNA_00226 at 243.246 kb on - strand, within CCNA_00226 at 243.259 kb on + strand, within CCNA_00226 at 243.275 kb on + strand, within CCNA_00226 at 243.275 kb on + strand, within CCNA_00226 at 243.276 kb on - strand, within CCNA_00226 at 243.283 kb on - strand, within CCNA_00226 at 243.304 kb on + strand, within CCNA_00226 at 243.400 kb on + strand, within CCNA_00226 at 243.424 kb on - strand at 243.431 kb on - strand at 243.434 kb on + strand at 243.449 kb on + strand at 243.449 kb on + strand at 243.449 kb on + strand at 243.457 kb on - strand at 243.457 kb on - strand at 243.457 kb on - strand at 243.457 kb on - strand at 243.462 kb on + strand at 243.470 kb on - strand at 243.479 kb on - strand at 243.489 kb on - strand at 243.489 kb on - strand at 243.495 kb on - strand at 243.528 kb on - strand at 243.539 kb on + strand at 243.547 kb on - strand, within CCNA_00227 at 243.557 kb on - strand, within CCNA_00227 at 243.557 kb on - strand, within CCNA_00227 at 243.557 kb on - strand, within CCNA_00227 at 243.598 kb on - strand, within CCNA_00227 at 243.620 kb on + strand, within CCNA_00227 at 243.647 kb on - strand, within CCNA_00227 at 243.647 kb on - strand, within CCNA_00227 at 243.673 kb on - strand, within CCNA_00227 at 243.708 kb on + strand, within CCNA_00227 at 243.729 kb on + strand, within CCNA_00227 at 243.729 kb on + strand, within CCNA_00227 at 243.729 kb on + strand, within CCNA_00227 at 243.737 kb on - strand, within CCNA_00227 at 243.751 kb on - strand, within CCNA_00227 at 243.756 kb on - strand, within CCNA_00227 at 243.756 kb on - strand, within CCNA_00227 at 243.767 kb on + strand, within CCNA_00227 at 243.783 kb on - strand, within CCNA_00227 at 243.783 kb on - strand, within CCNA_00227 at 243.844 kb on + strand, within CCNA_00227 at 243.848 kb on - strand, within CCNA_00227 at 243.869 kb on + strand, within CCNA_00227 at 243.932 kb on - strand, within CCNA_00227 at 243.932 kb on - strand, within CCNA_00227 at 243.939 kb on - strand, within CCNA_00227 at 243.955 kb on + strand, within CCNA_00227 at 243.956 kb on - strand, within CCNA_00227 at 243.977 kb on - strand, within CCNA_00227 at 244.016 kb on + strand at 244.016 kb on + strand at 244.022 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Nalidixic 0.06 mg/ml remove 242,164 + CCNA_00224 0.74 +2.1 242,164 + CCNA_00224 0.74 +0.2 242,164 - CCNA_00224 0.74 +0.7 242,170 - CCNA_00224 0.74 +1.7 242,172 - CCNA_00224 0.74 +0.2 242,172 - CCNA_00224 0.74 -1.2 242,188 + CCNA_00224 0.75 +1.5 242,196 - CCNA_00224 0.76 -1.5 242,209 + CCNA_00224 0.76 +0.2 242,224 - CCNA_00224 0.77 +0.3 242,224 - CCNA_00224 0.77 +0.1 242,250 + CCNA_00224 0.78 +1.3 242,264 + CCNA_00224 0.79 -0.4 242,300 + CCNA_00224 0.81 +0.6 242,305 + CCNA_00224 0.81 +2.0 242,338 + CCNA_00224 0.83 +0.3 242,343 + CCNA_00224 0.83 +0.0 242,373 - CCNA_00224 0.84 +1.8 242,428 - CCNA_00224 0.87 +0.6 242,441 - CCNA_00224 0.88 +0.5 242,441 - CCNA_00224 0.88 -0.6 242,481 + CCNA_00224 0.90 -0.2 242,489 - +1.2 242,527 + -0.8 242,527 + -0.1 242,527 + -1.4 242,527 + +0.4 242,527 + -0.2 242,527 + +3.3 242,527 + +0.5 242,527 + +1.4 242,527 + +0.3 242,527 + +0.3 242,527 + +0.2 242,535 - -0.6 242,535 - +0.5 242,535 - -0.3 242,535 - +2.0 242,535 - +0.5 242,565 - +0.8 242,573 - +1.6 242,587 + +0.9 242,587 + +0.2 242,595 - +1.5 242,600 - -1.0 242,643 + +0.3 242,656 - +1.5 242,675 - -0.1 242,683 - -0.9 242,704 + +0.9 242,715 + +2.3 242,716 - +0.5 242,718 - -0.8 242,723 - -0.8 242,723 - -0.4 242,892 + -1.1 242,892 + -1.0 242,892 + -0.6 242,892 + +1.9 242,892 + -1.0 242,892 + -0.2 242,900 - +2.2 242,900 - -1.2 242,900 - -0.5 242,900 - +1.3 242,900 - -1.4 242,900 - +3.2 242,900 - +1.5 242,900 - +3.1 242,900 - -1.1 242,904 + -1.2 242,932 + -0.0 242,987 + -1.5 242,995 - -0.1 243,010 + -0.9 243,018 - +1.2 243,020 + -1.2 243,028 - CCNA_00225 0.12 -1.5 243,045 + CCNA_00225 0.64 -0.2 243,050 + CCNA_00225 0.79 -1.6 243,050 + CCNA_00225 0.79 -1.5 243,068 - -0.7 243,083 + -1.6 243,091 - +1.2 243,154 + -0.2 243,162 - +0.5 243,162 - -1.6 243,162 - -0.2 243,162 - +0.0 243,164 + -2.1 243,164 + -0.3 243,164 + +3.0 243,198 + CCNA_00226 0.15 -1.5 243,198 + CCNA_00226 0.15 -0.9 243,202 - CCNA_00226 0.16 +0.3 243,205 + CCNA_00226 0.17 -0.0 243,206 - CCNA_00226 0.18 -1.6 243,213 + CCNA_00226 0.20 -0.8 243,246 - CCNA_00226 0.32 +0.1 243,259 + CCNA_00226 0.37 -2.0 243,275 + CCNA_00226 0.43 -2.7 243,275 + CCNA_00226 0.43 -1.3 243,276 - CCNA_00226 0.43 +1.4 243,283 - CCNA_00226 0.46 +0.9 243,304 + CCNA_00226 0.53 +1.9 243,400 + CCNA_00226 0.88 +0.9 243,424 - -0.0 243,431 - +0.3 243,434 + +0.3 243,449 + -2.9 243,449 + -1.7 243,449 + +0.5 243,457 - +1.6 243,457 - +0.0 243,457 - +0.5 243,457 - -0.9 243,462 + -2.7 243,470 - -2.1 243,479 - -0.3 243,489 - +0.7 243,489 - -1.2 243,495 - -1.0 243,528 - -1.6 243,539 + -1.4 243,547 - CCNA_00227 0.10 -1.5 243,557 - CCNA_00227 0.12 -5.1 243,557 - CCNA_00227 0.12 -0.7 243,557 - CCNA_00227 0.12 -1.8 243,598 - CCNA_00227 0.19 -1.0 243,620 + CCNA_00227 0.23 -0.8 243,647 - CCNA_00227 0.28 -0.8 243,647 - CCNA_00227 0.28 -0.2 243,673 - CCNA_00227 0.32 -2.4 243,708 + CCNA_00227 0.38 +0.9 243,729 + CCNA_00227 0.42 +0.3 243,729 + CCNA_00227 0.42 -2.0 243,729 + CCNA_00227 0.42 -1.3 243,737 - CCNA_00227 0.44 +1.2 243,751 - CCNA_00227 0.46 -0.8 243,756 - CCNA_00227 0.47 +0.5 243,756 - CCNA_00227 0.47 -2.0 243,767 + CCNA_00227 0.49 -2.6 243,783 - CCNA_00227 0.52 -0.9 243,783 - CCNA_00227 0.52 +0.9 243,844 + CCNA_00227 0.62 -0.1 243,848 - CCNA_00227 0.63 -0.7 243,869 + CCNA_00227 0.67 +0.5 243,932 - CCNA_00227 0.78 -1.9 243,932 - CCNA_00227 0.78 +0.7 243,939 - CCNA_00227 0.79 -2.1 243,955 + CCNA_00227 0.82 +0.2 243,956 - CCNA_00227 0.82 -1.3 243,977 - CCNA_00227 0.86 -0.2 244,016 + -0.2 244,016 + -1.2 244,022 + +0.7
Or see this region's nucleotide sequence