Experiment: PYE with Nalidixic 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00222 and CCNA_00223 are separated by 8 nucleotides CCNA_00223 and CCNA_00224 overlap by 4 nucleotides CCNA_00224 and CCNA_00225 are separated by 330 nucleotides CCNA_00225 and CCNA_00226 are separated by 100 nucleotides CCNA_00226 and CCNA_00227 are separated by 57 nucleotides
CCNA_00222: CCNA_00222 - glucose-6-phosphate isomerase/glucose-6 phosphate 1-epimerase, at 238,241 to 239,860
_00222
CCNA_00223: CCNA_00223 - esterase lipase Family protein, at 239,869 to 240,651
_00223
CCNA_00224: CCNA_00224 - TonB-dependent receptor protein, at 240,648 to 242,693
_00224
CCNA_00225: CCNA_00225 - hypothetical protein, at 243,024 to 243,056
_00225
CCNA_00226: CCNA_00226 - hypothetical protein, at 243,157 to 243,432
_00226
CCNA_00227: CCNA_00227 - methyltransferase, at 243,490 to 244,056
_00227
Position (kb) 240
241
242
243 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 239.814 kb on + strand at 239.878 kb on + strand at 239.929 kb on - strand at 239.955 kb on + strand, within CCNA_00223 at 240.014 kb on - strand, within CCNA_00223 at 240.028 kb on + strand, within CCNA_00223 at 240.036 kb on + strand, within CCNA_00223 at 240.036 kb on - strand, within CCNA_00223 at 240.042 kb on - strand, within CCNA_00223 at 240.050 kb on + strand, within CCNA_00223 at 240.058 kb on - strand, within CCNA_00223 at 240.061 kb on + strand, within CCNA_00223 at 240.061 kb on + strand, within CCNA_00223 at 240.069 kb on - strand, within CCNA_00223 at 240.074 kb on - strand, within CCNA_00223 at 240.082 kb on + strand, within CCNA_00223 at 240.105 kb on - strand, within CCNA_00223 at 240.152 kb on - strand, within CCNA_00223 at 240.152 kb on - strand, within CCNA_00223 at 240.162 kb on - strand, within CCNA_00223 at 240.223 kb on + strand, within CCNA_00223 at 240.269 kb on - strand, within CCNA_00223 at 240.312 kb on + strand, within CCNA_00223 at 240.312 kb on + strand, within CCNA_00223 at 240.312 kb on + strand, within CCNA_00223 at 240.318 kb on + strand, within CCNA_00223 at 240.320 kb on - strand, within CCNA_00223 at 240.320 kb on - strand, within CCNA_00223 at 240.320 kb on - strand, within CCNA_00223 at 240.320 kb on - strand, within CCNA_00223 at 240.320 kb on - strand, within CCNA_00223 at 240.326 kb on - strand, within CCNA_00223 at 240.328 kb on + strand, within CCNA_00223 at 240.397 kb on + strand, within CCNA_00223 at 240.404 kb on + strand, within CCNA_00223 at 240.430 kb on - strand, within CCNA_00223 at 240.460 kb on - strand, within CCNA_00223 at 240.468 kb on + strand, within CCNA_00223 at 240.468 kb on + strand, within CCNA_00223 at 240.468 kb on + strand, within CCNA_00223 at 240.498 kb on + strand, within CCNA_00223 at 240.503 kb on + strand, within CCNA_00223 at 240.511 kb on - strand, within CCNA_00223 at 240.516 kb on + strand, within CCNA_00223 at 240.524 kb on - strand, within CCNA_00223 at 240.524 kb on - strand, within CCNA_00223 at 240.531 kb on + strand, within CCNA_00223 at 240.547 kb on + strand, within CCNA_00223 at 240.555 kb on - strand, within CCNA_00223 at 240.674 kb on + strand at 240.674 kb on + strand at 240.674 kb on + strand at 240.682 kb on - strand at 240.682 kb on - strand at 240.702 kb on + strand at 240.710 kb on - strand at 240.710 kb on - strand at 240.710 kb on - strand at 240.710 kb on - strand at 240.710 kb on - strand at 240.741 kb on + strand at 240.749 kb on - strand at 240.749 kb on - strand at 240.762 kb on + strand at 240.791 kb on - strand at 240.815 kb on + strand at 240.822 kb on - strand at 240.822 kb on - strand at 240.834 kb on - strand at 240.840 kb on + strand at 240.848 kb on - strand at 240.864 kb on + strand, within CCNA_00224 at 240.864 kb on + strand, within CCNA_00224 at 240.869 kb on + strand, within CCNA_00224 at 240.923 kb on + strand, within CCNA_00224 at 240.925 kb on + strand, within CCNA_00224 at 240.933 kb on - strand, within CCNA_00224 at 240.942 kb on + strand, within CCNA_00224 at 241.017 kb on + strand, within CCNA_00224 at 241.039 kb on - strand, within CCNA_00224 at 241.039 kb on - strand, within CCNA_00224 at 241.106 kb on - strand, within CCNA_00224 at 241.116 kb on - strand, within CCNA_00224 at 241.228 kb on + strand, within CCNA_00224 at 241.261 kb on + strand, within CCNA_00224 at 241.284 kb on - strand, within CCNA_00224 at 241.303 kb on + strand, within CCNA_00224 at 241.319 kb on + strand, within CCNA_00224 at 241.338 kb on - strand, within CCNA_00224 at 241.353 kb on - strand, within CCNA_00224 at 241.422 kb on - strand, within CCNA_00224 at 241.430 kb on - strand, within CCNA_00224 at 241.437 kb on + strand, within CCNA_00224 at 241.438 kb on + strand, within CCNA_00224 at 241.470 kb on - strand, within CCNA_00224 at 241.488 kb on + strand, within CCNA_00224 at 241.548 kb on - strand, within CCNA_00224 at 241.548 kb on - strand, within CCNA_00224 at 241.548 kb on - strand, within CCNA_00224 at 241.553 kb on - strand, within CCNA_00224 at 241.565 kb on + strand, within CCNA_00224 at 241.565 kb on + strand, within CCNA_00224 at 241.567 kb on + strand, within CCNA_00224 at 241.573 kb on + strand, within CCNA_00224 at 241.575 kb on - strand, within CCNA_00224 at 241.581 kb on - strand, within CCNA_00224 at 241.581 kb on - strand, within CCNA_00224 at 241.585 kb on - strand, within CCNA_00224 at 241.598 kb on - strand, within CCNA_00224 at 241.626 kb on - strand, within CCNA_00224 at 241.636 kb on + strand, within CCNA_00224 at 241.644 kb on + strand, within CCNA_00224 at 241.644 kb on + strand, within CCNA_00224 at 241.652 kb on - strand, within CCNA_00224 at 241.652 kb on - strand, within CCNA_00224 at 241.652 kb on - strand, within CCNA_00224 at 241.681 kb on + strand, within CCNA_00224 at 241.681 kb on + strand, within CCNA_00224 at 241.683 kb on - strand, within CCNA_00224 at 241.689 kb on - strand, within CCNA_00224 at 241.739 kb on - strand, within CCNA_00224 at 241.749 kb on - strand, within CCNA_00224 at 241.762 kb on + strand, within CCNA_00224 at 241.770 kb on - strand, within CCNA_00224 at 241.778 kb on + strand at 241.791 kb on + strand, within CCNA_00224 at 241.826 kb on - strand, within CCNA_00224 at 241.829 kb on - strand, within CCNA_00224 at 241.843 kb on + strand, within CCNA_00224 at 241.851 kb on - strand, within CCNA_00224 at 241.851 kb on - strand, within CCNA_00224 at 241.866 kb on + strand, within CCNA_00224 at 241.872 kb on + strand, within CCNA_00224 at 241.875 kb on - strand, within CCNA_00224 at 241.978 kb on - strand, within CCNA_00224 at 242.004 kb on + strand, within CCNA_00224 at 242.164 kb on + strand, within CCNA_00224 at 242.164 kb on + strand, within CCNA_00224 at 242.164 kb on - strand, within CCNA_00224 at 242.170 kb on - strand, within CCNA_00224 at 242.172 kb on - strand, within CCNA_00224 at 242.172 kb on - strand, within CCNA_00224 at 242.188 kb on + strand, within CCNA_00224 at 242.196 kb on - strand, within CCNA_00224 at 242.209 kb on + strand, within CCNA_00224 at 242.224 kb on - strand, within CCNA_00224 at 242.224 kb on - strand, within CCNA_00224 at 242.250 kb on + strand, within CCNA_00224 at 242.264 kb on + strand, within CCNA_00224 at 242.300 kb on + strand, within CCNA_00224 at 242.305 kb on + strand, within CCNA_00224 at 242.338 kb on + strand, within CCNA_00224 at 242.343 kb on + strand, within CCNA_00224 at 242.373 kb on - strand, within CCNA_00224 at 242.428 kb on - strand, within CCNA_00224 at 242.441 kb on - strand, within CCNA_00224 at 242.441 kb on - strand, within CCNA_00224 at 242.481 kb on + strand, within CCNA_00224 at 242.489 kb on - strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.527 kb on + strand at 242.535 kb on - strand at 242.535 kb on - strand at 242.535 kb on - strand at 242.535 kb on - strand at 242.535 kb on - strand at 242.565 kb on - strand at 242.573 kb on - strand at 242.587 kb on + strand at 242.587 kb on + strand at 242.595 kb on - strand at 242.600 kb on - strand at 242.643 kb on + strand at 242.656 kb on - strand at 242.675 kb on - strand at 242.683 kb on - strand at 242.704 kb on + strand at 242.715 kb on + strand at 242.716 kb on - strand at 242.718 kb on - strand at 242.723 kb on - strand at 242.723 kb on - strand at 242.892 kb on + strand at 242.892 kb on + strand at 242.892 kb on + strand at 242.892 kb on + strand at 242.892 kb on + strand at 242.892 kb on + strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.900 kb on - strand at 242.904 kb on + strand at 242.932 kb on + strand at 242.987 kb on + strand at 242.995 kb on - strand at 243.010 kb on + strand at 243.018 kb on - strand at 243.020 kb on + strand at 243.028 kb on - strand, within CCNA_00225 at 243.045 kb on + strand, within CCNA_00225 at 243.050 kb on + strand, within CCNA_00225 at 243.050 kb on + strand, within CCNA_00225 at 243.068 kb on - strand at 243.083 kb on + strand at 243.091 kb on - strand at 243.154 kb on + strand at 243.162 kb on - strand at 243.162 kb on - strand at 243.162 kb on - strand at 243.162 kb on - strand at 243.164 kb on + strand at 243.164 kb on + strand at 243.164 kb on + strand at 243.198 kb on + strand, within CCNA_00226 at 243.198 kb on + strand, within CCNA_00226 at 243.202 kb on - strand, within CCNA_00226 at 243.205 kb on + strand, within CCNA_00226 at 243.206 kb on - strand, within CCNA_00226 at 243.213 kb on + strand, within CCNA_00226 at 243.246 kb on - strand, within CCNA_00226 at 243.259 kb on + strand, within CCNA_00226 at 243.275 kb on + strand, within CCNA_00226 at 243.275 kb on + strand, within CCNA_00226 at 243.276 kb on - strand, within CCNA_00226 at 243.283 kb on - strand, within CCNA_00226 at 243.304 kb on + strand, within CCNA_00226 at 243.400 kb on + strand, within CCNA_00226 at 243.424 kb on - strand at 243.431 kb on - strand at 243.434 kb on + strand at 243.449 kb on + strand at 243.449 kb on + strand at 243.449 kb on + strand at 243.457 kb on - strand at 243.457 kb on - strand at 243.457 kb on - strand at 243.457 kb on - strand at 243.462 kb on + strand at 243.470 kb on - strand at 243.479 kb on - strand at 243.489 kb on - strand at 243.489 kb on - strand at 243.495 kb on - strand at 243.528 kb on - strand at 243.539 kb on + strand at 243.547 kb on - strand, within CCNA_00227 at 243.557 kb on - strand, within CCNA_00227 at 243.557 kb on - strand, within CCNA_00227 at 243.557 kb on - strand, within CCNA_00227 at 243.598 kb on - strand, within CCNA_00227 at 243.620 kb on + strand, within CCNA_00227 at 243.647 kb on - strand, within CCNA_00227 at 243.647 kb on - strand, within CCNA_00227 at 243.673 kb on - strand, within CCNA_00227
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Nalidixic 0.06 mg/ml remove 239,814 + +0.2 239,878 + -0.4 239,929 - -1.5 239,955 + CCNA_00223 0.11 +1.3 240,014 - CCNA_00223 0.19 +0.5 240,028 + CCNA_00223 0.20 -0.2 240,036 + CCNA_00223 0.21 -2.7 240,036 - CCNA_00223 0.21 +0.5 240,042 - CCNA_00223 0.22 +0.3 240,050 + CCNA_00223 0.23 +0.6 240,058 - CCNA_00223 0.24 +0.3 240,061 + CCNA_00223 0.25 -2.1 240,061 + CCNA_00223 0.25 +2.4 240,069 - CCNA_00223 0.26 -0.7 240,074 - CCNA_00223 0.26 +1.7 240,082 + CCNA_00223 0.27 +0.8 240,105 - CCNA_00223 0.30 -1.3 240,152 - CCNA_00223 0.36 -0.7 240,152 - CCNA_00223 0.36 +0.3 240,162 - CCNA_00223 0.37 -0.5 240,223 + CCNA_00223 0.45 -0.4 240,269 - CCNA_00223 0.51 +1.3 240,312 + CCNA_00223 0.57 -0.6 240,312 + CCNA_00223 0.57 -0.3 240,312 + CCNA_00223 0.57 -1.6 240,318 + CCNA_00223 0.57 -0.0 240,320 - CCNA_00223 0.58 +0.5 240,320 - CCNA_00223 0.58 -1.4 240,320 - CCNA_00223 0.58 +0.7 240,320 - CCNA_00223 0.58 -0.3 240,320 - CCNA_00223 0.58 -0.2 240,326 - CCNA_00223 0.58 -1.3 240,328 + CCNA_00223 0.59 -0.1 240,397 + CCNA_00223 0.67 -0.9 240,404 + CCNA_00223 0.68 +0.9 240,430 - CCNA_00223 0.72 +3.6 240,460 - CCNA_00223 0.75 +1.4 240,468 + CCNA_00223 0.77 +0.8 240,468 + CCNA_00223 0.77 -2.6 240,468 + CCNA_00223 0.77 -0.2 240,498 + CCNA_00223 0.80 +0.3 240,503 + CCNA_00223 0.81 +1.4 240,511 - CCNA_00223 0.82 +0.0 240,516 + CCNA_00223 0.83 +0.7 240,524 - CCNA_00223 0.84 +2.0 240,524 - CCNA_00223 0.84 -0.1 240,531 + CCNA_00223 0.85 -1.9 240,547 + CCNA_00223 0.87 -1.1 240,555 - CCNA_00223 0.88 -0.2 240,674 + +0.9 240,674 + -0.9 240,674 + +1.9 240,682 - +0.5 240,682 - -0.0 240,702 + +0.5 240,710 - +0.6 240,710 - -0.4 240,710 - +0.3 240,710 - +1.6 240,710 - -0.2 240,741 + -2.7 240,749 - -0.0 240,749 - +0.7 240,762 + -0.4 240,791 - +0.7 240,815 + +0.5 240,822 - +1.1 240,822 - -0.5 240,834 - +2.6 240,840 + +2.3 240,848 - -1.1 240,864 + CCNA_00224 0.11 -0.6 240,864 + CCNA_00224 0.11 +0.0 240,869 + CCNA_00224 0.11 -0.5 240,923 + CCNA_00224 0.13 +0.8 240,925 + CCNA_00224 0.14 -1.5 240,933 - CCNA_00224 0.14 +0.7 240,942 + CCNA_00224 0.14 -1.1 241,017 + CCNA_00224 0.18 -2.0 241,039 - CCNA_00224 0.19 -0.2 241,039 - CCNA_00224 0.19 -0.2 241,106 - CCNA_00224 0.22 +1.1 241,116 - CCNA_00224 0.23 +0.4 241,228 + CCNA_00224 0.28 -0.1 241,261 + CCNA_00224 0.30 +0.7 241,284 - CCNA_00224 0.31 -0.1 241,303 + CCNA_00224 0.32 +0.5 241,319 + CCNA_00224 0.33 +2.7 241,338 - CCNA_00224 0.34 +1.2 241,353 - CCNA_00224 0.34 -0.2 241,422 - CCNA_00224 0.38 +0.6 241,430 - CCNA_00224 0.38 -1.7 241,437 + CCNA_00224 0.39 -1.2 241,438 + CCNA_00224 0.39 -1.8 241,470 - CCNA_00224 0.40 +1.0 241,488 + CCNA_00224 0.41 +2.4 241,548 - CCNA_00224 0.44 +0.9 241,548 - CCNA_00224 0.44 +0.9 241,548 - CCNA_00224 0.44 -0.7 241,553 - CCNA_00224 0.44 +2.1 241,565 + CCNA_00224 0.45 -0.0 241,565 + CCNA_00224 0.45 +0.4 241,567 + CCNA_00224 0.45 -0.5 241,573 + CCNA_00224 0.45 +0.2 241,575 - CCNA_00224 0.45 +2.6 241,581 - CCNA_00224 0.46 +0.2 241,581 - CCNA_00224 0.46 +0.3 241,585 - CCNA_00224 0.46 +0.3 241,598 - CCNA_00224 0.46 +0.7 241,626 - CCNA_00224 0.48 +0.4 241,636 + CCNA_00224 0.48 +1.2 241,644 + CCNA_00224 0.49 +0.4 241,644 + CCNA_00224 0.49 -0.1 241,652 - CCNA_00224 0.49 +0.1 241,652 - CCNA_00224 0.49 +0.9 241,652 - CCNA_00224 0.49 +0.4 241,681 + CCNA_00224 0.50 -0.8 241,681 + CCNA_00224 0.50 +1.3 241,683 - CCNA_00224 0.51 -0.4 241,689 - CCNA_00224 0.51 +1.1 241,739 - CCNA_00224 0.53 +0.7 241,749 - CCNA_00224 0.54 +0.5 241,762 + CCNA_00224 0.54 +3.2 241,770 - CCNA_00224 0.55 +1.1 241,778 + +0.2 241,791 + CCNA_00224 0.56 +0.9 241,826 - CCNA_00224 0.58 +0.5 241,829 - CCNA_00224 0.58 -0.2 241,843 + CCNA_00224 0.58 -1.1 241,851 - CCNA_00224 0.59 -0.5 241,851 - CCNA_00224 0.59 -0.1 241,866 + CCNA_00224 0.60 -0.2 241,872 + CCNA_00224 0.60 +1.0 241,875 - CCNA_00224 0.60 +0.3 241,978 - CCNA_00224 0.65 -0.9 242,004 + CCNA_00224 0.66 -1.4 242,164 + CCNA_00224 0.74 +2.1 242,164 + CCNA_00224 0.74 +0.2 242,164 - CCNA_00224 0.74 +0.7 242,170 - CCNA_00224 0.74 +1.7 242,172 - CCNA_00224 0.74 +0.2 242,172 - CCNA_00224 0.74 -1.2 242,188 + CCNA_00224 0.75 +1.5 242,196 - CCNA_00224 0.76 -1.5 242,209 + CCNA_00224 0.76 +0.2 242,224 - CCNA_00224 0.77 +0.3 242,224 - CCNA_00224 0.77 +0.1 242,250 + CCNA_00224 0.78 +1.3 242,264 + CCNA_00224 0.79 -0.4 242,300 + CCNA_00224 0.81 +0.6 242,305 + CCNA_00224 0.81 +2.0 242,338 + CCNA_00224 0.83 +0.3 242,343 + CCNA_00224 0.83 +0.0 242,373 - CCNA_00224 0.84 +1.8 242,428 - CCNA_00224 0.87 +0.6 242,441 - CCNA_00224 0.88 +0.5 242,441 - CCNA_00224 0.88 -0.6 242,481 + CCNA_00224 0.90 -0.2 242,489 - +1.2 242,527 + -0.8 242,527 + -0.1 242,527 + -1.4 242,527 + +0.4 242,527 + -0.2 242,527 + +3.3 242,527 + +0.5 242,527 + +1.4 242,527 + +0.3 242,527 + +0.3 242,527 + +0.2 242,535 - -0.6 242,535 - +0.5 242,535 - -0.3 242,535 - +2.0 242,535 - +0.5 242,565 - +0.8 242,573 - +1.6 242,587 + +0.9 242,587 + +0.2 242,595 - +1.5 242,600 - -1.0 242,643 + +0.3 242,656 - +1.5 242,675 - -0.1 242,683 - -0.9 242,704 + +0.9 242,715 + +2.3 242,716 - +0.5 242,718 - -0.8 242,723 - -0.8 242,723 - -0.4 242,892 + -1.1 242,892 + -1.0 242,892 + -0.6 242,892 + +1.9 242,892 + -1.0 242,892 + -0.2 242,900 - +2.2 242,900 - -1.2 242,900 - -0.5 242,900 - +1.3 242,900 - -1.4 242,900 - +3.2 242,900 - +1.5 242,900 - +3.1 242,900 - -1.1 242,904 + -1.2 242,932 + -0.0 242,987 + -1.5 242,995 - -0.1 243,010 + -0.9 243,018 - +1.2 243,020 + -1.2 243,028 - CCNA_00225 0.12 -1.5 243,045 + CCNA_00225 0.64 -0.2 243,050 + CCNA_00225 0.79 -1.6 243,050 + CCNA_00225 0.79 -1.5 243,068 - -0.7 243,083 + -1.6 243,091 - +1.2 243,154 + -0.2 243,162 - +0.5 243,162 - -1.6 243,162 - -0.2 243,162 - +0.0 243,164 + -2.1 243,164 + -0.3 243,164 + +3.0 243,198 + CCNA_00226 0.15 -1.5 243,198 + CCNA_00226 0.15 -0.9 243,202 - CCNA_00226 0.16 +0.3 243,205 + CCNA_00226 0.17 -0.0 243,206 - CCNA_00226 0.18 -1.6 243,213 + CCNA_00226 0.20 -0.8 243,246 - CCNA_00226 0.32 +0.1 243,259 + CCNA_00226 0.37 -2.0 243,275 + CCNA_00226 0.43 -2.7 243,275 + CCNA_00226 0.43 -1.3 243,276 - CCNA_00226 0.43 +1.4 243,283 - CCNA_00226 0.46 +0.9 243,304 + CCNA_00226 0.53 +1.9 243,400 + CCNA_00226 0.88 +0.9 243,424 - -0.0 243,431 - +0.3 243,434 + +0.3 243,449 + -2.9 243,449 + -1.7 243,449 + +0.5 243,457 - +1.6 243,457 - +0.0 243,457 - +0.5 243,457 - -0.9 243,462 + -2.7 243,470 - -2.1 243,479 - -0.3 243,489 - +0.7 243,489 - -1.2 243,495 - -1.0 243,528 - -1.6 243,539 + -1.4 243,547 - CCNA_00227 0.10 -1.5 243,557 - CCNA_00227 0.12 -5.1 243,557 - CCNA_00227 0.12 -0.7 243,557 - CCNA_00227 0.12 -1.8 243,598 - CCNA_00227 0.19 -1.0 243,620 + CCNA_00227 0.23 -0.8 243,647 - CCNA_00227 0.28 -0.8 243,647 - CCNA_00227 0.28 -0.2 243,673 - CCNA_00227 0.32 -2.4
Or see this region's nucleotide sequence