Experiment: Succinic Acid 31.25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A3546 and Rru_A3547 are separated by 87 nucleotides Rru_A3547 and Rru_A3548 are separated by 218 nucleotides
Rru_A3546: Rru_A3546 - Glutamine amidotransferase of anthranilate synthase (NCBI), at 4,083,456 to 4,085,642
_A3546
Rru_A3547: Rru_A3547 - hypothetical protein (NCBI), at 4,085,730 to 4,086,077
_A3547
Rru_A3548: Rru_A3548 - Magnesium-protoporphyrin IX monomethyl ester anaerobic oxidative cyclase (NCBI), at 4,086,296 to 4,087,903
_A3548
Position (kb)
4085
4086
4087 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 4084.791 kb on + strand, within Rru_A3546 at 4084.791 kb on + strand, within Rru_A3546 at 4084.792 kb on - strand, within Rru_A3546 at 4085.076 kb on - strand, within Rru_A3546 at 4085.089 kb on - strand, within Rru_A3546 at 4085.419 kb on - strand, within Rru_A3546 at 4085.419 kb on - strand, within Rru_A3546 at 4085.612 kb on - strand at 4085.641 kb on - strand at 4085.641 kb on - strand at 4085.672 kb on + strand at 4085.672 kb on + strand at 4085.672 kb on + strand at 4085.672 kb on + strand at 4085.672 kb on + strand at 4085.672 kb on + strand at 4085.672 kb on + strand at 4085.673 kb on - strand at 4085.673 kb on - strand at 4085.673 kb on - strand at 4085.673 kb on - strand at 4085.774 kb on + strand, within Rru_A3547 at 4085.774 kb on + strand, within Rru_A3547 at 4085.775 kb on - strand, within Rru_A3547 at 4085.775 kb on - strand, within Rru_A3547 at 4085.862 kb on + strand, within Rru_A3547 at 4085.889 kb on + strand, within Rru_A3547 at 4085.889 kb on + strand, within Rru_A3547 at 4085.892 kb on - strand, within Rru_A3547 at 4085.924 kb on + strand, within Rru_A3547 at 4085.924 kb on + strand, within Rru_A3547 at 4085.924 kb on + strand, within Rru_A3547 at 4085.925 kb on - strand, within Rru_A3547 at 4085.925 kb on - strand, within Rru_A3547 at 4085.953 kb on - strand, within Rru_A3547 at 4085.957 kb on - strand, within Rru_A3547 at 4085.957 kb on - strand, within Rru_A3547 at 4086.075 kb on + strand at 4086.076 kb on - strand at 4086.295 kb on + strand at 4086.295 kb on + strand at 4086.296 kb on - strand at 4086.296 kb on - strand at 4086.296 kb on - strand at 4086.302 kb on - strand at 4086.323 kb on + strand at 4086.323 kb on + strand at 4086.323 kb on + strand at 4086.323 kb on + strand at 4086.323 kb on + strand at 4086.323 kb on + strand at 4086.324 kb on - strand at 4086.407 kb on + strand at 4086.527 kb on + strand, within Rru_A3548 at 4086.527 kb on + strand, within Rru_A3548 at 4086.528 kb on - strand, within Rru_A3548 at 4086.528 kb on - strand, within Rru_A3548 at 4086.588 kb on + strand, within Rru_A3548 at 4086.589 kb on - strand, within Rru_A3548 at 4086.752 kb on + strand, within Rru_A3548 at 4086.851 kb on + strand, within Rru_A3548 at 4086.851 kb on + strand, within Rru_A3548 at 4086.851 kb on + strand, within Rru_A3548 at 4086.852 kb on - strand, within Rru_A3548 at 4086.852 kb on - strand, within Rru_A3548 at 4086.852 kb on - strand, within Rru_A3548 at 4086.852 kb on - strand, within Rru_A3548 at 4086.852 kb on - strand, within Rru_A3548 at 4086.852 kb on - strand, within Rru_A3548 at 4086.852 kb on - strand, within Rru_A3548 at 4086.852 kb on - strand, within Rru_A3548 at 4086.857 kb on + strand, within Rru_A3548 at 4086.858 kb on - strand, within Rru_A3548 at 4086.858 kb on - strand, within Rru_A3548 at 4086.858 kb on - strand, within Rru_A3548 at 4086.893 kb on - strand, within Rru_A3548
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid 31.25 mM; anaerobic remove 4,084,791 + Rru_A3546 0.61 -1.8 4,084,791 + Rru_A3546 0.61 -0.3 4,084,792 - Rru_A3546 0.61 -2.7 4,085,076 - Rru_A3546 0.74 -0.1 4,085,089 - Rru_A3546 0.75 -0.8 4,085,419 - Rru_A3546 0.90 -0.5 4,085,419 - Rru_A3546 0.90 -0.6 4,085,612 - +0.9 4,085,641 - -0.2 4,085,641 - +0.5 4,085,672 + -0.0 4,085,672 + -3.7 4,085,672 + -0.5 4,085,672 + -0.7 4,085,672 + -0.9 4,085,672 + +0.7 4,085,672 + -0.2 4,085,673 - +0.1 4,085,673 - -0.0 4,085,673 - -0.0 4,085,673 - +1.0 4,085,774 + Rru_A3547 0.13 -3.1 4,085,774 + Rru_A3547 0.13 -2.6 4,085,775 - Rru_A3547 0.13 -1.9 4,085,775 - Rru_A3547 0.13 -0.5 4,085,862 + Rru_A3547 0.38 +4.2 4,085,889 + Rru_A3547 0.46 -2.3 4,085,889 + Rru_A3547 0.46 +0.3 4,085,892 - Rru_A3547 0.47 -2.2 4,085,924 + Rru_A3547 0.56 -3.0 4,085,924 + Rru_A3547 0.56 -0.2 4,085,924 + Rru_A3547 0.56 -1.4 4,085,925 - Rru_A3547 0.56 -1.8 4,085,925 - Rru_A3547 0.56 -0.2 4,085,953 - Rru_A3547 0.64 -0.3 4,085,957 - Rru_A3547 0.65 -2.7 4,085,957 - Rru_A3547 0.65 -1.4 4,086,075 + -2.6 4,086,076 - -0.1 4,086,295 + -1.9 4,086,295 + +0.8 4,086,296 - -1.2 4,086,296 - -0.8 4,086,296 - -1.9 4,086,302 - -2.1 4,086,323 + -2.7 4,086,323 + -2.5 4,086,323 + -1.9 4,086,323 + -1.5 4,086,323 + -1.5 4,086,323 + -0.5 4,086,324 - +0.8 4,086,407 + -2.0 4,086,527 + Rru_A3548 0.14 -1.2 4,086,527 + Rru_A3548 0.14 -0.7 4,086,528 - Rru_A3548 0.14 -2.3 4,086,528 - Rru_A3548 0.14 -3.5 4,086,588 + Rru_A3548 0.18 -2.3 4,086,589 - Rru_A3548 0.18 -3.2 4,086,752 + Rru_A3548 0.28 +0.5 4,086,851 + Rru_A3548 0.35 -2.7 4,086,851 + Rru_A3548 0.35 -0.8 4,086,851 + Rru_A3548 0.35 -2.4 4,086,852 - Rru_A3548 0.35 -3.2 4,086,852 - Rru_A3548 0.35 -0.2 4,086,852 - Rru_A3548 0.35 +0.0 4,086,852 - Rru_A3548 0.35 -1.4 4,086,852 - Rru_A3548 0.35 -2.0 4,086,852 - Rru_A3548 0.35 -2.4 4,086,852 - Rru_A3548 0.35 -3.8 4,086,852 - Rru_A3548 0.35 -3.5 4,086,857 + Rru_A3548 0.35 -0.4 4,086,858 - Rru_A3548 0.35 -1.4 4,086,858 - Rru_A3548 0.35 -3.2 4,086,858 - Rru_A3548 0.35 -1.4 4,086,893 - Rru_A3548 0.37 -1.8
Or see this region's nucleotide sequence