Experiment: Succinic Acid 31.25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2015 and Rru_A2016 overlap by 4 nucleotides Rru_A2016 and Rru_AR0038 are separated by 73 nucleotides Rru_AR0038 and Rru_A2017 are separated by 126 nucleotides Rru_A2017 and Rru_A2018 are separated by 81 nucleotides Rru_A2018 and Rru_A2019 are separated by 188 nucleotides Rru_A2019 and Rru_A2020 are separated by 13 nucleotides
Rru_A2015: Rru_A2015 - chaperone DnaJ (NCBI), at 2,330,185 to 2,331,186
_A2015
Rru_A2016: Rru_A2016 - hypothetical protein (NCBI), at 2,331,183 to 2,331,521
_A2016
Rru_AR0038: Rru_AR0038 - tRNA-Met (NCBI), at 2,331,595 to 2,331,671
_AR0038
Rru_A2017: Rru_A2017 - hypothetical protein (NCBI), at 2,331,798 to 2,332,040
_A2017
Rru_A2018: Rru_A2018 - hypothetical protein (NCBI), at 2,332,122 to 2,332,511
_A2018
Rru_A2019: Rru_A2019 - Plasmid maintenance system antidote protein (NCBI), at 2,332,700 to 2,332,999
_A2019
Rru_A2020: Rru_A2020 - Plasmid maintenance system killer (NCBI), at 2,333,013 to 2,333,294
_A2020
Position (kb)
2331
2332
2333 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2331.107 kb on - strand at 2331.107 kb on - strand at 2331.107 kb on - strand at 2331.107 kb on - strand at 2331.107 kb on - strand at 2331.107 kb on - strand at 2331.175 kb on - strand at 2331.175 kb on - strand at 2331.175 kb on - strand at 2331.190 kb on + strand at 2331.190 kb on + strand at 2331.190 kb on + strand at 2331.190 kb on + strand at 2331.191 kb on - strand at 2331.191 kb on - strand at 2331.191 kb on - strand at 2331.191 kb on - strand at 2331.191 kb on - strand at 2331.191 kb on - strand at 2331.247 kb on + strand, within Rru_A2016 at 2331.248 kb on - strand, within Rru_A2016 at 2331.575 kb on + strand at 2331.676 kb on + strand at 2331.677 kb on - strand at 2331.677 kb on - strand at 2331.808 kb on + strand at 2331.809 kb on - strand at 2331.809 kb on - strand at 2331.832 kb on - strand, within Rru_A2017 at 2331.856 kb on + strand, within Rru_A2017 at 2331.867 kb on - strand, within Rru_A2017 at 2331.867 kb on - strand, within Rru_A2017 at 2331.955 kb on + strand, within Rru_A2017 at 2331.955 kb on + strand, within Rru_A2017 at 2331.956 kb on - strand, within Rru_A2017 at 2332.029 kb on + strand at 2332.030 kb on - strand at 2332.076 kb on + strand at 2332.077 kb on - strand at 2332.077 kb on - strand at 2332.077 kb on - strand at 2332.134 kb on + strand at 2332.135 kb on - strand at 2332.170 kb on + strand, within Rru_A2018 at 2332.556 kb on - strand at 2332.613 kb on + strand at 2332.613 kb on + strand at 2333.015 kb on - strand at 2333.021 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid 31.25 mM; anaerobic remove 2,331,107 - +0.0 2,331,107 - -0.4 2,331,107 - -0.6 2,331,107 - -3.7 2,331,107 - +0.3 2,331,107 - -0.6 2,331,175 - -1.2 2,331,175 - -0.3 2,331,175 - +0.7 2,331,190 + +0.2 2,331,190 + +0.7 2,331,190 + -1.0 2,331,190 + -0.1 2,331,191 - -0.2 2,331,191 - -0.1 2,331,191 - +1.0 2,331,191 - +0.5 2,331,191 - -2.4 2,331,191 - -1.5 2,331,247 + Rru_A2016 0.19 +0.1 2,331,248 - Rru_A2016 0.19 -0.7 2,331,575 + +0.4 2,331,676 + +0.5 2,331,677 - -0.2 2,331,677 - +2.3 2,331,808 + -3.5 2,331,809 - -0.2 2,331,809 - -1.1 2,331,832 - Rru_A2017 0.14 -0.4 2,331,856 + Rru_A2017 0.24 -0.5 2,331,867 - Rru_A2017 0.28 +0.5 2,331,867 - Rru_A2017 0.28 -0.1 2,331,955 + Rru_A2017 0.65 -2.0 2,331,955 + Rru_A2017 0.65 -1.6 2,331,956 - Rru_A2017 0.65 -1.5 2,332,029 + -0.4 2,332,030 - +0.3 2,332,076 + -0.6 2,332,077 - -2.1 2,332,077 - -0.4 2,332,077 - +0.9 2,332,134 + -0.4 2,332,135 - -0.1 2,332,170 + Rru_A2018 0.12 +0.7 2,332,556 - +0.2 2,332,613 + +0.1 2,332,613 + +0.2 2,333,015 - -0.1 2,333,021 - -0.5
Or see this region's nucleotide sequence