Experiment: Succinic Acid 31.25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1607 and Rru_A1608 are separated by 8 nucleotides Rru_A1608 and Rru_A1609 are separated by 86 nucleotides Rru_A1609 and Rru_A1610 are separated by 86 nucleotides
Rru_A1607: Rru_A1607 - 4-alpha-glucanotransferase (NCBI), at 1,898,895 to 1,901,081
_A1607
Rru_A1608: Rru_A1608 - MscS Mechanosensitive ion channel (NCBI), at 1,901,090 to 1,903,696
_A1608
Rru_A1609: Rru_A1609 - hypothetical protein (NCBI), at 1,903,783 to 1,904,085
_A1609
Rru_A1610: Rru_A1610 - hypothetical protein (NCBI), at 1,904,172 to 1,904,954
_A1610
Position (kb)
1901
1902
1903
1904 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1900.122 kb on + strand, within Rru_A1607 at 1900.122 kb on + strand, within Rru_A1607 at 1900.291 kb on - strand, within Rru_A1607 at 1900.416 kb on + strand, within Rru_A1607 at 1900.416 kb on + strand, within Rru_A1607 at 1900.416 kb on + strand, within Rru_A1607 at 1900.417 kb on - strand, within Rru_A1607 at 1900.558 kb on - strand, within Rru_A1607 at 1900.615 kb on - strand, within Rru_A1607 at 1900.889 kb on + strand at 1900.890 kb on - strand at 1900.890 kb on - strand at 1900.940 kb on + strand at 1901.039 kb on + strand at 1901.039 kb on + strand at 1901.039 kb on + strand at 1901.039 kb on + strand at 1901.039 kb on + strand at 1901.039 kb on + strand at 1901.040 kb on - strand at 1901.040 kb on - strand at 1901.040 kb on - strand at 1901.040 kb on - strand at 1901.040 kb on - strand at 1901.079 kb on + strand at 1901.080 kb on - strand at 1901.080 kb on - strand at 1901.080 kb on - strand at 1901.464 kb on + strand, within Rru_A1608 at 1901.464 kb on + strand, within Rru_A1608 at 1901.464 kb on + strand, within Rru_A1608 at 1901.465 kb on - strand, within Rru_A1608 at 1901.465 kb on - strand, within Rru_A1608 at 1901.465 kb on - strand, within Rru_A1608 at 1901.486 kb on + strand, within Rru_A1608 at 1901.486 kb on + strand, within Rru_A1608 at 1901.486 kb on + strand, within Rru_A1608 at 1901.486 kb on + strand, within Rru_A1608 at 1901.487 kb on - strand, within Rru_A1608 at 1901.489 kb on - strand, within Rru_A1608 at 1902.464 kb on + strand, within Rru_A1608 at 1902.605 kb on + strand, within Rru_A1608 at 1902.606 kb on - strand, within Rru_A1608 at 1902.606 kb on - strand, within Rru_A1608 at 1902.926 kb on + strand, within Rru_A1608 at 1902.928 kb on + strand, within Rru_A1608 at 1902.929 kb on - strand, within Rru_A1608 at 1903.178 kb on - strand, within Rru_A1608 at 1903.285 kb on + strand, within Rru_A1608 at 1903.285 kb on + strand, within Rru_A1608 at 1903.285 kb on + strand, within Rru_A1608 at 1903.285 kb on + strand, within Rru_A1608 at 1903.285 kb on + strand, within Rru_A1608 at 1903.286 kb on - strand, within Rru_A1608 at 1903.286 kb on - strand, within Rru_A1608 at 1903.424 kb on + strand, within Rru_A1608 at 1903.424 kb on + strand, within Rru_A1608 at 1903.424 kb on + strand, within Rru_A1608 at 1903.424 kb on + strand, within Rru_A1608 at 1903.425 kb on - strand, within Rru_A1608 at 1903.425 kb on - strand, within Rru_A1608 at 1903.425 kb on - strand, within Rru_A1608 at 1903.426 kb on + strand, within Rru_A1608 at 1903.427 kb on - strand, within Rru_A1608 at 1903.717 kb on - strand at 1903.768 kb on + strand at 1903.768 kb on + strand at 1903.769 kb on - strand at 1903.798 kb on + strand at 1903.799 kb on - strand at 1903.799 kb on - strand at 1904.083 kb on + strand at 1904.083 kb on + strand at 1904.083 kb on + strand at 1904.376 kb on - strand, within Rru_A1610
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid 31.25 mM; anaerobic remove 1,900,122 + Rru_A1607 0.56 +2.4 1,900,122 + Rru_A1607 0.56 +2.2 1,900,291 - Rru_A1607 0.64 +2.6 1,900,416 + Rru_A1607 0.70 +2.2 1,900,416 + Rru_A1607 0.70 +2.1 1,900,416 + Rru_A1607 0.70 +2.0 1,900,417 - Rru_A1607 0.70 +2.6 1,900,558 - Rru_A1607 0.76 +2.2 1,900,615 - Rru_A1607 0.79 +2.5 1,900,889 + +3.0 1,900,890 - +0.3 1,900,890 - +2.8 1,900,940 + +1.6 1,901,039 + +1.7 1,901,039 + +2.4 1,901,039 + +1.6 1,901,039 + +2.3 1,901,039 + +1.4 1,901,039 + +1.6 1,901,040 - +0.7 1,901,040 - +1.2 1,901,040 - +1.6 1,901,040 - +1.9 1,901,040 - +0.9 1,901,079 + +0.8 1,901,080 - -1.1 1,901,080 - -3.1 1,901,080 - -0.2 1,901,464 + Rru_A1608 0.14 -1.7 1,901,464 + Rru_A1608 0.14 -4.0 1,901,464 + Rru_A1608 0.14 -1.9 1,901,465 - Rru_A1608 0.14 -2.0 1,901,465 - Rru_A1608 0.14 +0.4 1,901,465 - Rru_A1608 0.14 -0.1 1,901,486 + Rru_A1608 0.15 -1.7 1,901,486 + Rru_A1608 0.15 -2.5 1,901,486 + Rru_A1608 0.15 -0.9 1,901,486 + Rru_A1608 0.15 -1.5 1,901,487 - Rru_A1608 0.15 -1.3 1,901,489 - Rru_A1608 0.15 -3.4 1,902,464 + Rru_A1608 0.53 -0.9 1,902,605 + Rru_A1608 0.58 -0.5 1,902,606 - Rru_A1608 0.58 -1.2 1,902,606 - Rru_A1608 0.58 +0.5 1,902,926 + Rru_A1608 0.70 +1.4 1,902,928 + Rru_A1608 0.71 +0.5 1,902,929 - Rru_A1608 0.71 -0.9 1,903,178 - Rru_A1608 0.80 -0.9 1,903,285 + Rru_A1608 0.84 -2.5 1,903,285 + Rru_A1608 0.84 -2.1 1,903,285 + Rru_A1608 0.84 -0.8 1,903,285 + Rru_A1608 0.84 -1.4 1,903,285 + Rru_A1608 0.84 -1.7 1,903,286 - Rru_A1608 0.84 -0.8 1,903,286 - Rru_A1608 0.84 -2.4 1,903,424 + Rru_A1608 0.90 -1.3 1,903,424 + Rru_A1608 0.90 -0.1 1,903,424 + Rru_A1608 0.90 -2.0 1,903,424 + Rru_A1608 0.90 -3.1 1,903,425 - Rru_A1608 0.90 -2.4 1,903,425 - Rru_A1608 0.90 -1.3 1,903,425 - Rru_A1608 0.90 -1.4 1,903,426 + Rru_A1608 0.90 -1.0 1,903,427 - Rru_A1608 0.90 -1.4 1,903,717 - -2.5 1,903,768 + -1.5 1,903,768 + -0.8 1,903,769 - +0.3 1,903,798 + +0.4 1,903,799 - +0.3 1,903,799 - +0.1 1,904,083 + -1.2 1,904,083 + -1.5 1,904,083 + -1.1 1,904,376 - Rru_A1610 0.26 +1.8
Or see this region's nucleotide sequence