Experiment: Succinic Acid 31.25 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1324 and Rru_A1325 are separated by 41 nucleotides Rru_A1325 and Rru_A1326 are separated by 33 nucleotides Rru_A1326 and Rru_A1327 are separated by 2 nucleotides Rru_A1327 and Rru_A1328 are separated by 45 nucleotides
Rru_A1324: Rru_A1324 - beta subunit of malonate decarboxylase (NCBI), at 1,562,704 to 1,563,660
_A1324
Rru_A1325: Rru_A1325 - hypothetical protein (NCBI), at 1,563,702 to 1,564,595
_A1325
Rru_A1326: Rru_A1326 - hypothetical protein (NCBI), at 1,564,629 to 1,565,729
_A1326
Rru_A1327: Rru_A1327 - hypothetical protein (NCBI), at 1,565,732 to 1,566,484
_A1327
Rru_A1328: Rru_A1328 - Biotin/lipoyl attachment (NCBI), at 1,566,530 to 1,566,739
_A1328
Position (kb) 1564
1565
1566 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1563.677 kb on + strand at 1563.678 kb on - strand at 1563.782 kb on + strand at 1563.783 kb on - strand at 1563.918 kb on + strand, within Rru_A1325 at 1563.918 kb on + strand, within Rru_A1325 at 1563.918 kb on + strand, within Rru_A1325 at 1563.919 kb on - strand, within Rru_A1325 at 1563.948 kb on + strand, within Rru_A1325 at 1563.948 kb on + strand, within Rru_A1325 at 1563.948 kb on + strand, within Rru_A1325 at 1563.949 kb on - strand, within Rru_A1325 at 1563.949 kb on - strand, within Rru_A1325 at 1563.969 kb on + strand, within Rru_A1325 at 1563.970 kb on - strand, within Rru_A1325 at 1563.970 kb on - strand, within Rru_A1325 at 1563.970 kb on - strand, within Rru_A1325 at 1564.101 kb on + strand, within Rru_A1325 at 1564.102 kb on - strand, within Rru_A1325 at 1564.578 kb on + strand at 1564.579 kb on - strand at 1564.601 kb on + strand at 1564.602 kb on - strand at 1564.788 kb on + strand, within Rru_A1326 at 1564.788 kb on + strand, within Rru_A1326 at 1564.788 kb on + strand, within Rru_A1326 at 1564.788 kb on + strand, within Rru_A1326 at 1564.788 kb on + strand, within Rru_A1326 at 1564.930 kb on - strand, within Rru_A1326 at 1564.930 kb on - strand, within Rru_A1326 at 1564.943 kb on + strand, within Rru_A1326 at 1564.944 kb on - strand, within Rru_A1326 at 1565.205 kb on - strand, within Rru_A1326 at 1565.220 kb on + strand, within Rru_A1326 at 1565.221 kb on - strand, within Rru_A1326 at 1565.221 kb on - strand, within Rru_A1326 at 1565.276 kb on + strand, within Rru_A1326 at 1565.276 kb on + strand, within Rru_A1326 at 1565.276 kb on + strand, within Rru_A1326 at 1565.277 kb on - strand, within Rru_A1326 at 1565.310 kb on + strand, within Rru_A1326 at 1565.311 kb on - strand, within Rru_A1326 at 1565.406 kb on + strand, within Rru_A1326 at 1565.424 kb on + strand, within Rru_A1326 at 1565.425 kb on - strand, within Rru_A1326 at 1565.425 kb on - strand, within Rru_A1326 at 1565.433 kb on + strand, within Rru_A1326 at 1565.433 kb on + strand, within Rru_A1326 at 1565.434 kb on - strand, within Rru_A1326 at 1565.434 kb on - strand, within Rru_A1326 at 1565.728 kb on - strand at 1565.728 kb on - strand at 1565.728 kb on - strand at 1565.749 kb on + strand at 1565.761 kb on + strand at 1566.164 kb on + strand, within Rru_A1327 at 1566.165 kb on - strand, within Rru_A1327 at 1566.529 kb on + strand at 1566.530 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid 31.25 mM; anaerobic remove 1,563,677 + -2.1 1,563,678 - +0.6 1,563,782 + +0.4 1,563,783 - -0.6 1,563,918 + Rru_A1325 0.24 -1.6 1,563,918 + Rru_A1325 0.24 -0.8 1,563,918 + Rru_A1325 0.24 -2.5 1,563,919 - Rru_A1325 0.24 +0.4 1,563,948 + Rru_A1325 0.28 -0.3 1,563,948 + Rru_A1325 0.28 +0.0 1,563,948 + Rru_A1325 0.28 -2.1 1,563,949 - Rru_A1325 0.28 +0.5 1,563,949 - Rru_A1325 0.28 -2.1 1,563,969 + Rru_A1325 0.30 -2.0 1,563,970 - Rru_A1325 0.30 +1.0 1,563,970 - Rru_A1325 0.30 -1.0 1,563,970 - Rru_A1325 0.30 -0.7 1,564,101 + Rru_A1325 0.45 -0.5 1,564,102 - Rru_A1325 0.45 -0.8 1,564,578 + -2.3 1,564,579 - +0.6 1,564,601 + -1.3 1,564,602 - -0.2 1,564,788 + Rru_A1326 0.14 -0.4 1,564,788 + Rru_A1326 0.14 -1.1 1,564,788 + Rru_A1326 0.14 -2.3 1,564,788 + Rru_A1326 0.14 -0.6 1,564,788 + Rru_A1326 0.14 +0.4 1,564,930 - Rru_A1326 0.27 -0.0 1,564,930 - Rru_A1326 0.27 -1.7 1,564,943 + Rru_A1326 0.29 -3.5 1,564,944 - Rru_A1326 0.29 +1.5 1,565,205 - Rru_A1326 0.52 -0.0 1,565,220 + Rru_A1326 0.54 -2.5 1,565,221 - Rru_A1326 0.54 -0.4 1,565,221 - Rru_A1326 0.54 -0.9 1,565,276 + Rru_A1326 0.59 -0.9 1,565,276 + Rru_A1326 0.59 -1.9 1,565,276 + Rru_A1326 0.59 -0.6 1,565,277 - Rru_A1326 0.59 -0.5 1,565,310 + Rru_A1326 0.62 -3.9 1,565,311 - Rru_A1326 0.62 -0.5 1,565,406 + Rru_A1326 0.71 -0.1 1,565,424 + Rru_A1326 0.72 -1.1 1,565,425 - Rru_A1326 0.72 -0.7 1,565,425 - Rru_A1326 0.72 -2.4 1,565,433 + Rru_A1326 0.73 -2.2 1,565,433 + Rru_A1326 0.73 -1.0 1,565,434 - Rru_A1326 0.73 -0.3 1,565,434 - Rru_A1326 0.73 -0.4 1,565,728 - +0.5 1,565,728 - -0.4 1,565,728 - -2.1 1,565,749 + -1.1 1,565,761 + -2.3 1,566,164 + Rru_A1327 0.57 -2.2 1,566,165 - Rru_A1327 0.58 -0.4 1,566,529 + -2.2 1,566,530 - +0.1
Or see this region's nucleotide sequence