Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS09045 and MMP_RS03170 are separated by 120 nucleotides MMP_RS03170 and MMP_RS03175 are separated by 144 nucleotides MMP_RS03175 and MMP_RS03180 are separated by 8 nucleotides MMP_RS03180 and MMP_RS03185 are separated by 80 nucleotides
MMP_RS09045: MMP_RS09045 - class III signal peptide-containing protein, at 597,015 to 597,902
_RS09045
MMP_RS03170: MMP_RS03170 - orotidine-5'-phosphate decarboxylase, at 598,023 to 598,676
_RS03170
MMP_RS03175: MMP_RS03175 - translation initiation factor eIF-1A, at 598,821 to 599,135
_RS03175
MMP_RS03180: MMP_RS03180 - serine protein kinase RIO, at 599,144 to 599,965
_RS03180
MMP_RS03185: MMP_RS03185 - KH domain-containing protein, at 600,046 to 600,600
_RS03185
Position (kb) 598
599
600 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 597.840 kb on - strand at 597.855 kb on - strand at 597.855 kb on - strand at 597.902 kb on - strand at 597.902 kb on - strand at 597.903 kb on + strand at 597.903 kb on + strand at 597.906 kb on - strand at 597.920 kb on + strand at 597.958 kb on - strand at 597.965 kb on + strand at 597.965 kb on + strand at 597.965 kb on + strand at 597.965 kb on + strand at 597.966 kb on - strand at 597.966 kb on - strand at 597.967 kb on + strand at 597.969 kb on + strand at 597.982 kb on + strand at 598.013 kb on - strand at 598.014 kb on + strand at 598.024 kb on - strand at 598.025 kb on + strand at 598.411 kb on + strand at 598.533 kb on + strand at 598.677 kb on + strand at 598.689 kb on + strand at 598.863 kb on + strand at 599.116 kb on + strand at 599.499 kb on - strand at 599.527 kb on - strand at 599.701 kb on + strand at 599.777 kb on - strand at 600.004 kb on + strand at 600.066 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 remove 597,840 - +2.5 597,855 - +2.2 597,855 - +2.5 597,902 - +2.2 597,902 - +2.0 597,903 + +2.4 597,903 + +2.2 597,906 - +2.6 597,920 + +2.4 597,958 - +2.3 597,965 + +2.5 597,965 + +2.6 597,965 + +2.5 597,965 + +2.2 597,966 - +1.7 597,966 - +1.6 597,967 + +1.6 597,969 + +1.3 597,982 + +2.1 598,013 - +0.4 598,014 + +2.1 598,024 - +2.6 598,025 + -0.2 598,411 + -3.9 598,533 + -4.0 598,677 + -3.5 598,689 + -2.5 598,863 + -3.4 599,116 + -3.6 599,499 - -3.2 599,527 - -3.0 599,701 + -2.0 599,777 - -4.1 600,004 + -5.0 600,066 + -3.3
Or see this region's nucleotide sequence