Experiment: PYE with Nalidixic 0.03 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01580 and CCNA_01581 are separated by 108 nucleotides CCNA_01581 and CCNA_01582 are separated by 114 nucleotides CCNA_01582 and CCNA_01583 are separated by 69 nucleotides
CCNA_01580: CCNA_01580 - M15 superfamily membrane peptidase, at 1,696,883 to 1,697,533
_01580
CCNA_01581: CCNA_01581 - hypothetical protein, at 1,697,642 to 1,697,974
_01581
CCNA_01582: CCNA_01582 - Glutathione-dependent formaldehyde-activating enzyme, at 1,698,089 to 1,698,451
_01582
CCNA_01583: CCNA_01583 - phosphate-binding protein, at 1,698,521 to 1,699,561
_01583
CCNA_03954: CCNA_03954 - hypothetical protein, at 1,699,323 to 1,699,610
_03954
Position (kb)
1698
1699 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1697.091 kb on + strand, within CCNA_01580 at 1697.091 kb on + strand, within CCNA_01580 at 1697.091 kb on + strand, within CCNA_01580 at 1697.094 kb on - strand, within CCNA_01580 at 1697.098 kb on + strand, within CCNA_01580 at 1697.099 kb on - strand, within CCNA_01580 at 1697.147 kb on - strand, within CCNA_01580 at 1697.159 kb on + strand, within CCNA_01580 at 1697.182 kb on + strand, within CCNA_01580 at 1697.182 kb on + strand, within CCNA_01580 at 1697.216 kb on + strand, within CCNA_01580 at 1697.238 kb on + strand, within CCNA_01580 at 1697.246 kb on - strand, within CCNA_01580 at 1697.246 kb on - strand, within CCNA_01580 at 1697.246 kb on - strand, within CCNA_01580 at 1697.270 kb on + strand, within CCNA_01580 at 1697.277 kb on + strand, within CCNA_01580 at 1697.278 kb on - strand, within CCNA_01580 at 1697.278 kb on - strand, within CCNA_01580 at 1697.545 kb on + strand at 1697.584 kb on + strand at 1697.612 kb on - strand at 1697.634 kb on + strand at 1697.634 kb on + strand at 1697.644 kb on - strand at 1697.644 kb on - strand at 1697.651 kb on + strand at 1697.669 kb on + strand at 1697.760 kb on + strand, within CCNA_01581 at 1697.773 kb on - strand, within CCNA_01581 at 1697.829 kb on - strand, within CCNA_01581 at 1697.850 kb on - strand, within CCNA_01581 at 1697.850 kb on - strand, within CCNA_01581 at 1698.002 kb on - strand at 1698.018 kb on + strand at 1698.018 kb on + strand at 1698.126 kb on + strand, within CCNA_01582 at 1698.136 kb on + strand, within CCNA_01582 at 1698.237 kb on - strand, within CCNA_01582 at 1698.389 kb on - strand, within CCNA_01582 at 1698.396 kb on - strand, within CCNA_01582 at 1698.406 kb on + strand, within CCNA_01582 at 1698.421 kb on + strand at 1698.429 kb on - strand at 1698.721 kb on - strand, within CCNA_01583 at 1698.721 kb on - strand, within CCNA_01583 at 1698.762 kb on + strand, within CCNA_01583 at 1698.895 kb on - strand, within CCNA_01583 at 1699.027 kb on + strand, within CCNA_01583 at 1699.036 kb on + strand at 1699.044 kb on + strand, within CCNA_01583 at 1699.152 kb on - strand, within CCNA_01583 at 1699.164 kb on + strand, within CCNA_01583 at 1699.342 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Nalidixic 0.03 mg/ml remove 1,697,091 + CCNA_01580 0.32 +2.2 1,697,091 + CCNA_01580 0.32 +2.5 1,697,091 + CCNA_01580 0.32 +0.4 1,697,094 - CCNA_01580 0.32 +1.7 1,697,098 + CCNA_01580 0.33 +1.2 1,697,099 - CCNA_01580 0.33 -1.0 1,697,147 - CCNA_01580 0.41 -0.3 1,697,159 + CCNA_01580 0.42 +0.9 1,697,182 + CCNA_01580 0.46 +1.7 1,697,182 + CCNA_01580 0.46 +1.0 1,697,216 + CCNA_01580 0.51 +0.8 1,697,238 + CCNA_01580 0.55 +1.6 1,697,246 - CCNA_01580 0.56 -1.5 1,697,246 - CCNA_01580 0.56 +0.5 1,697,246 - CCNA_01580 0.56 -1.5 1,697,270 + CCNA_01580 0.59 +1.4 1,697,277 + CCNA_01580 0.61 +0.9 1,697,278 - CCNA_01580 0.61 +0.3 1,697,278 - CCNA_01580 0.61 +0.3 1,697,545 + +1.8 1,697,584 + +0.8 1,697,612 - +0.9 1,697,634 + +0.9 1,697,634 + +0.9 1,697,644 - -0.2 1,697,644 - +0.0 1,697,651 + +1.1 1,697,669 + -1.4 1,697,760 + CCNA_01581 0.35 +2.3 1,697,773 - CCNA_01581 0.39 -1.2 1,697,829 - CCNA_01581 0.56 -0.4 1,697,850 - CCNA_01581 0.62 +0.1 1,697,850 - CCNA_01581 0.62 -0.1 1,698,002 - -1.5 1,698,018 + -0.6 1,698,018 + +1.7 1,698,126 + CCNA_01582 0.10 -0.1 1,698,136 + CCNA_01582 0.13 -0.6 1,698,237 - CCNA_01582 0.41 +0.7 1,698,389 - CCNA_01582 0.83 -1.6 1,698,396 - CCNA_01582 0.85 -1.5 1,698,406 + CCNA_01582 0.87 -2.0 1,698,421 + +0.1 1,698,429 - +2.0 1,698,721 - CCNA_01583 0.19 +1.0 1,698,721 - CCNA_01583 0.19 -0.6 1,698,762 + CCNA_01583 0.23 -0.3 1,698,895 - CCNA_01583 0.36 +0.1 1,699,027 + CCNA_01583 0.49 +0.3 1,699,036 + +1.5 1,699,044 + CCNA_01583 0.50 +0.2 1,699,152 - CCNA_01583 0.61 -0.3 1,699,164 + CCNA_01583 0.62 +0.8 1,699,342 - +0.5
Or see this region's nucleotide sequence