Experiment: Hexanoic 21 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2988 and Rru_A2989 are separated by 9 nucleotides Rru_A2989 and Rru_A2990 overlap by 4 nucleotides Rru_A2990 and Rru_A2991 overlap by 4 nucleotides Rru_A2991 and Rru_A2992 are separated by 252 nucleotides
Rru_A2988: Rru_A2988 - Precorrin-6X reductase (NCBI), at 3,441,006 to 3,441,761
_A2988
Rru_A2989: Rru_A2989 - Precorrin-3B C17-methyltransferase region (NCBI), at 3,441,771 to 3,442,547
_A2989
Rru_A2990: Rru_A2990 - precorrin-2 C20-methyltransferase (NCBI), at 3,442,544 to 3,443,278
_A2990
Rru_A2991: Rru_A2991 - Precorrin-8X methylmutase (NCBI), at 3,443,275 to 3,443,901
_A2991
Rru_A2992: Rru_A2992 - Precorrin-6Y C5,15-methyltransferase (decarboxylating) (NCBI), at 3,444,154 to 3,445,380
_A2992
Position (kb)
3442
3443
3444 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3441.791 kb on - strand at 3441.803 kb on + strand at 3441.803 kb on + strand at 3441.803 kb on + strand at 3441.804 kb on - strand at 3441.804 kb on - strand at 3441.804 kb on - strand at 3441.804 kb on - strand at 3441.805 kb on + strand at 3441.805 kb on + strand at 3441.805 kb on + strand at 3441.806 kb on - strand at 3441.806 kb on - strand at 3441.806 kb on - strand at 3441.982 kb on + strand, within Rru_A2989 at 3441.983 kb on - strand, within Rru_A2989 at 3442.045 kb on + strand, within Rru_A2989 at 3442.430 kb on - strand, within Rru_A2989 at 3442.430 kb on - strand, within Rru_A2989 at 3442.437 kb on - strand, within Rru_A2989 at 3442.438 kb on + strand, within Rru_A2989 at 3442.438 kb on + strand, within Rru_A2989 at 3442.439 kb on - strand, within Rru_A2989 at 3442.439 kb on - strand, within Rru_A2989 at 3442.659 kb on + strand, within Rru_A2990 at 3442.659 kb on + strand, within Rru_A2990 at 3442.659 kb on + strand, within Rru_A2990 at 3442.660 kb on - strand, within Rru_A2990 at 3442.660 kb on - strand, within Rru_A2990 at 3442.703 kb on + strand, within Rru_A2990 at 3442.704 kb on - strand, within Rru_A2990 at 3442.920 kb on + strand, within Rru_A2990 at 3442.921 kb on - strand, within Rru_A2990 at 3442.921 kb on - strand, within Rru_A2990 at 3442.921 kb on - strand, within Rru_A2990 at 3442.921 kb on - strand, within Rru_A2990 at 3442.935 kb on + strand, within Rru_A2990 at 3442.935 kb on + strand, within Rru_A2990 at 3443.019 kb on + strand, within Rru_A2990 at 3443.020 kb on - strand, within Rru_A2990 at 3443.044 kb on - strand, within Rru_A2990 at 3443.044 kb on - strand, within Rru_A2990 at 3443.044 kb on - strand, within Rru_A2990 at 3443.082 kb on + strand, within Rru_A2990 at 3443.082 kb on + strand, within Rru_A2990 at 3443.083 kb on - strand, within Rru_A2990 at 3443.083 kb on - strand, within Rru_A2990 at 3443.167 kb on + strand, within Rru_A2990 at 3443.168 kb on - strand, within Rru_A2990 at 3443.169 kb on + strand, within Rru_A2990 at 3443.170 kb on - strand, within Rru_A2990 at 3443.170 kb on - strand, within Rru_A2990 at 3443.170 kb on - strand, within Rru_A2990 at 3443.170 kb on - strand, within Rru_A2990 at 3443.360 kb on - strand, within Rru_A2991 at 3443.360 kb on - strand, within Rru_A2991 at 3443.871 kb on + strand at 3443.871 kb on + strand at 3443.871 kb on + strand at 3443.872 kb on - strand at 3443.891 kb on + strand at 3443.892 kb on - strand at 3443.961 kb on + strand at 3443.961 kb on + strand at 3443.961 kb on + strand at 3443.961 kb on + strand at 3443.966 kb on + strand at 3443.968 kb on + strand at 3443.968 kb on + strand at 3443.968 kb on + strand at 3444.109 kb on + strand at 3444.110 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Hexanoic 21 mM; anaerobic remove 3,441,791 - +0.6 3,441,803 + -3.5 3,441,803 + +1.1 3,441,803 + +0.9 3,441,804 - -2.1 3,441,804 - -0.1 3,441,804 - -1.3 3,441,804 - -0.4 3,441,805 + -1.1 3,441,805 + +0.6 3,441,805 + +2.4 3,441,806 - -1.0 3,441,806 - -0.7 3,441,806 - -0.5 3,441,982 + Rru_A2989 0.27 -3.2 3,441,983 - Rru_A2989 0.27 -1.1 3,442,045 + Rru_A2989 0.35 +0.5 3,442,430 - Rru_A2989 0.85 -1.6 3,442,430 - Rru_A2989 0.85 -0.4 3,442,437 - Rru_A2989 0.86 -3.9 3,442,438 + Rru_A2989 0.86 +1.3 3,442,438 + Rru_A2989 0.86 -1.6 3,442,439 - Rru_A2989 0.86 -0.6 3,442,439 - Rru_A2989 0.86 -0.7 3,442,659 + Rru_A2990 0.16 +0.3 3,442,659 + Rru_A2990 0.16 -3.2 3,442,659 + Rru_A2990 0.16 -1.7 3,442,660 - Rru_A2990 0.16 -1.3 3,442,660 - Rru_A2990 0.16 +0.1 3,442,703 + Rru_A2990 0.22 -1.1 3,442,704 - Rru_A2990 0.22 -0.3 3,442,920 + Rru_A2990 0.51 -0.9 3,442,921 - Rru_A2990 0.51 -1.5 3,442,921 - Rru_A2990 0.51 -3.4 3,442,921 - Rru_A2990 0.51 -0.7 3,442,921 - Rru_A2990 0.51 -2.9 3,442,935 + Rru_A2990 0.53 -1.7 3,442,935 + Rru_A2990 0.53 -0.4 3,443,019 + Rru_A2990 0.65 -0.6 3,443,020 - Rru_A2990 0.65 +0.6 3,443,044 - Rru_A2990 0.68 +0.0 3,443,044 - Rru_A2990 0.68 -1.6 3,443,044 - Rru_A2990 0.68 -1.7 3,443,082 + Rru_A2990 0.73 -3.0 3,443,082 + Rru_A2990 0.73 +1.1 3,443,083 - Rru_A2990 0.73 -1.1 3,443,083 - Rru_A2990 0.73 -2.7 3,443,167 + Rru_A2990 0.85 -1.7 3,443,168 - Rru_A2990 0.85 -0.5 3,443,169 + Rru_A2990 0.85 -0.6 3,443,170 - Rru_A2990 0.85 +2.0 3,443,170 - Rru_A2990 0.85 -0.6 3,443,170 - Rru_A2990 0.85 -0.2 3,443,170 - Rru_A2990 0.85 -3.9 3,443,360 - Rru_A2991 0.14 -2.5 3,443,360 - Rru_A2991 0.14 -0.3 3,443,871 + -0.3 3,443,871 + -1.9 3,443,871 + -1.2 3,443,872 - +0.4 3,443,891 + -1.4 3,443,892 - -3.0 3,443,961 + -1.9 3,443,961 + -0.9 3,443,961 + -1.9 3,443,961 + +0.0 3,443,966 + -4.3 3,443,968 + +0.6 3,443,968 + -0.2 3,443,968 + -1.6 3,444,109 + -0.7 3,444,110 - -0.0
Or see this region's nucleotide sequence