Experiment: Hexanoic 21 mM; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2027 and Rru_A2028 overlap by 4 nucleotides Rru_A2028 and Rru_A2029 are separated by 78 nucleotides Rru_A2029 and Rru_A2030 are separated by 327 nucleotides Rru_A2030 and Rru_A2031 overlap by 49 nucleotides
Rru_A2027: Rru_A2027 - Aminotransferase, class V (NCBI), at 2,340,150 to 2,341,256
_A2027
Rru_A2028: Rru_A2028 - Transcriptional Regulator, BadM/Rrf2 family (NCBI), at 2,341,253 to 2,341,717
_A2028
Rru_A2029: Rru_A2029 - Serine O-acetyltransferase (NCBI), at 2,341,796 to 2,342,560
_A2029
Rru_A2030: Rru_A2030 - hypothetical protein (NCBI), at 2,342,888 to 2,343,550
_A2030
Rru_A2031: Rru_A2031 - Protein of unknown function UPF0075 (NCBI), at 2,343,502 to 2,344,629
_A2031
Position (kb)
2341
2342
2343 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2341.238 kb on - strand at 2341.238 kb on - strand at 2341.238 kb on - strand at 2342.001 kb on + strand, within Rru_A2029 at 2342.001 kb on + strand, within Rru_A2029 at 2342.699 kb on + strand at 2342.707 kb on + strand at 2342.726 kb on + strand at 2342.726 kb on + strand at 2342.806 kb on + strand at 2342.868 kb on + strand at 2342.868 kb on + strand at 2343.548 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Hexanoic 21 mM; anaerobic remove 2,341,238 - -0.4 2,341,238 - +0.7 2,341,238 - -0.5 2,342,001 + Rru_A2029 0.27 -0.8 2,342,001 + Rru_A2029 0.27 -2.3 2,342,699 + +1.5 2,342,707 + -0.1 2,342,726 + +1.0 2,342,726 + +0.3 2,342,806 + +0.9 2,342,868 + -3.2 2,342,868 + +0.2 2,343,548 + -0.3
Or see this region's nucleotide sequence