Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_5011 and Psyr_5012 are separated by 32 nucleotides Psyr_5012 and Psyr_RNA78 are separated by 117 nucleotides Psyr_RNA78 and Psyr_RNA79 are separated by 108 nucleotides Psyr_RNA79 and Psyr_RNA80 are separated by 144 nucleotides
Psyr_5011: Psyr_5011 - Amino acid adenylation:Thioester reductase, at 5,943,655 to 5,947,101
_5011
Psyr_5012: Psyr_5012 - conserved hypothetical protein, at 5,947,134 to 5,947,613
_5012
Psyr_RNA78: Psyr_RNA78 - 5S ribosomal RNA, at 5,947,731 to 5,947,845
_RNA78
Psyr_RNA79: Psyr_RNA79 - 5S ribosomal RNA, at 5,947,954 to 5,948,068
_RNA79
Psyr_RNA80: Psyr_RNA80 - 23S ribosomal RNA, at 5,948,213 to 5,951,115
_RNA80
Position (kb) 5947
5948 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 5946.826 kb on + strand at 5946.826 kb on + strand at 5946.943 kb on + strand at 5946.943 kb on + strand at 5946.944 kb on - strand at 5946.944 kb on - strand at 5946.944 kb on - strand at 5947.033 kb on + strand at 5947.107 kb on + strand at 5947.119 kb on + strand at 5947.119 kb on + strand at 5947.119 kb on + strand at 5947.119 kb on + strand at 5947.119 kb on + strand at 5947.119 kb on + strand at 5947.119 kb on + strand at 5947.120 kb on - strand at 5947.120 kb on - strand at 5947.279 kb on + strand, within Psyr_5012 at 5947.280 kb on - strand, within Psyr_5012 at 5947.398 kb on + strand, within Psyr_5012 at 5947.398 kb on + strand, within Psyr_5012 at 5947.398 kb on + strand, within Psyr_5012 at 5947.398 kb on + strand, within Psyr_5012 at 5947.398 kb on + strand, within Psyr_5012 at 5947.398 kb on + strand, within Psyr_5012 at 5947.398 kb on + strand, within Psyr_5012 at 5947.398 kb on + strand, within Psyr_5012 at 5947.398 kb on + strand, within Psyr_5012 at 5947.398 kb on + strand, within Psyr_5012 at 5947.398 kb on + strand, within Psyr_5012 at 5947.398 kb on + strand, within Psyr_5012 at 5947.398 kb on + strand, within Psyr_5012 at 5947.398 kb on + strand, within Psyr_5012 at 5947.398 kb on + strand, within Psyr_5012 at 5947.398 kb on + strand, within Psyr_5012 at 5947.398 kb on + strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.399 kb on - strand, within Psyr_5012 at 5947.400 kb on + strand, within Psyr_5012 at 5947.400 kb on + strand, within Psyr_5012 at 5947.400 kb on + strand, within Psyr_5012 at 5947.400 kb on + strand, within Psyr_5012 at 5947.400 kb on + strand, within Psyr_5012 at 5947.400 kb on + strand, within Psyr_5012 at 5947.400 kb on + strand, within Psyr_5012 at 5947.400 kb on + strand, within Psyr_5012 at 5947.401 kb on - strand, within Psyr_5012 at 5947.401 kb on - strand, within Psyr_5012 at 5947.401 kb on - strand, within Psyr_5012 at 5947.401 kb on - strand, within Psyr_5012 at 5947.401 kb on - strand, within Psyr_5012 at 5947.401 kb on - strand, within Psyr_5012 at 5947.401 kb on - strand, within Psyr_5012 at 5947.401 kb on - strand, within Psyr_5012 at 5947.401 kb on - strand, within Psyr_5012 at 5947.401 kb on - strand, within Psyr_5012 at 5947.485 kb on - strand, within Psyr_5012 at 5947.590 kb on - strand at 5947.666 kb on + strand at 5947.666 kb on + strand at 5947.745 kb on - strand, within Psyr_RNA78 at 5947.745 kb on - strand, within Psyr_RNA78 at 5947.745 kb on - strand, within Psyr_RNA78 at 5947.745 kb on - strand, within Psyr_RNA78 at 5947.745 kb on - strand, within Psyr_RNA78 at 5947.745 kb on - strand, within Psyr_RNA78 at 5947.782 kb on + strand, within Psyr_RNA78 at 5947.833 kb on + strand, within Psyr_RNA78 at 5947.833 kb on + strand, within Psyr_RNA78 at 5947.834 kb on - strand at 5947.834 kb on - strand at 5947.874 kb on - strand at 5947.874 kb on - strand at 5947.901 kb on + strand at 5947.901 kb on + strand at 5947.902 kb on - strand at 5947.902 kb on - strand at 5947.902 kb on - strand at 5947.902 kb on - strand at 5947.902 kb on - strand at 5947.902 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 5,946,826 + +0.6 5,946,826 + +2.3 5,946,943 + +0.4 5,946,943 + +1.5 5,946,944 - +0.1 5,946,944 - -0.2 5,946,944 - -1.5 5,947,033 + -1.7 5,947,107 + -0.6 5,947,119 + +2.9 5,947,119 + -1.0 5,947,119 + -1.9 5,947,119 + -0.1 5,947,119 + -0.2 5,947,119 + +1.3 5,947,119 + -1.7 5,947,120 - -0.9 5,947,120 - +0.0 5,947,279 + Psyr_5012 0.30 +4.8 5,947,280 - Psyr_5012 0.30 -0.9 5,947,398 + Psyr_5012 0.55 +3.9 5,947,398 + Psyr_5012 0.55 -0.8 5,947,398 + Psyr_5012 0.55 +2.9 5,947,398 + Psyr_5012 0.55 +3.9 5,947,398 + Psyr_5012 0.55 -2.7 5,947,398 + Psyr_5012 0.55 -3.2 5,947,398 + Psyr_5012 0.55 -1.5 5,947,398 + Psyr_5012 0.55 -0.8 5,947,398 + Psyr_5012 0.55 +1.9 5,947,398 + Psyr_5012 0.55 -2.0 5,947,398 + Psyr_5012 0.55 -0.2 5,947,398 + Psyr_5012 0.55 -1.1 5,947,398 + Psyr_5012 0.55 +0.5 5,947,398 + Psyr_5012 0.55 -2.1 5,947,398 + Psyr_5012 0.55 +3.5 5,947,398 + Psyr_5012 0.55 +0.6 5,947,398 + Psyr_5012 0.55 +3.9 5,947,399 - Psyr_5012 0.55 -0.4 5,947,399 - Psyr_5012 0.55 +1.3 5,947,399 - Psyr_5012 0.55 -0.6 5,947,399 - Psyr_5012 0.55 -3.6 5,947,399 - Psyr_5012 0.55 -0.7 5,947,399 - Psyr_5012 0.55 -1.5 5,947,399 - Psyr_5012 0.55 +2.6 5,947,399 - Psyr_5012 0.55 +2.3 5,947,399 - Psyr_5012 0.55 -1.3 5,947,399 - Psyr_5012 0.55 -1.4 5,947,399 - Psyr_5012 0.55 -1.5 5,947,399 - Psyr_5012 0.55 +0.5 5,947,399 - Psyr_5012 0.55 +2.6 5,947,399 - Psyr_5012 0.55 -1.8 5,947,399 - Psyr_5012 0.55 +1.3 5,947,399 - Psyr_5012 0.55 +1.3 5,947,399 - Psyr_5012 0.55 -0.2 5,947,399 - Psyr_5012 0.55 +0.3 5,947,400 + Psyr_5012 0.55 +1.3 5,947,400 + Psyr_5012 0.55 +1.9 5,947,400 + Psyr_5012 0.55 +1.3 5,947,400 + Psyr_5012 0.55 -0.7 5,947,400 + Psyr_5012 0.55 +0.2 5,947,400 + Psyr_5012 0.55 -3.6 5,947,400 + Psyr_5012 0.55 -1.2 5,947,400 + Psyr_5012 0.55 +3.7 5,947,401 - Psyr_5012 0.56 -0.6 5,947,401 - Psyr_5012 0.56 -1.5 5,947,401 - Psyr_5012 0.56 -0.2 5,947,401 - Psyr_5012 0.56 -1.3 5,947,401 - Psyr_5012 0.56 +0.5 5,947,401 - Psyr_5012 0.56 -3.2 5,947,401 - Psyr_5012 0.56 +0.3 5,947,401 - Psyr_5012 0.56 -1.6 5,947,401 - Psyr_5012 0.56 -1.5 5,947,401 - Psyr_5012 0.56 -0.6 5,947,485 - Psyr_5012 0.73 +0.3 5,947,590 - -0.2 5,947,666 + +1.9 5,947,666 + +3.5 5,947,745 - Psyr_RNA78 0.12 +3.3 5,947,745 - Psyr_RNA78 0.12 -0.9 5,947,745 - Psyr_RNA78 0.12 -0.4 5,947,745 - Psyr_RNA78 0.12 -0.0 5,947,745 - Psyr_RNA78 0.12 +2.6 5,947,745 - Psyr_RNA78 0.12 +1.2 5,947,782 + Psyr_RNA78 0.44 +1.9 5,947,833 + Psyr_RNA78 0.89 +2.3 5,947,833 + Psyr_RNA78 0.89 +3.7 5,947,834 - -0.2 5,947,834 - -0.5 5,947,874 - +2.3 5,947,874 - +4.6 5,947,901 + -2.7 5,947,901 + -1.5 5,947,902 - -1.4 5,947,902 - +2.6 5,947,902 - +3.5 5,947,902 - +3.3 5,947,902 - +0.1 5,947,902 - +2.5
Or see this region's nucleotide sequence