Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3982 and Psyr_RNA61 are separated by 158 nucleotides Psyr_RNA61 and Psyr_3983 are separated by 361 nucleotides
Psyr_3982: Psyr_3982 - 3-hydroxyacyl-CoA-acyl carrier protein transferase, at 4,735,007 to 4,735,888
_3982
Psyr_RNA61: Psyr_RNA61 - tRNA-Gly, at 4,736,047 to 4,736,120
_RNA61
Psyr_3983: Psyr_3983 - Serralysin, at 4,736,482 to 4,738,839
_3983
Position (kb) 4736
4737 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 4735.055 kb on + strand at 4735.056 kb on - strand at 4735.181 kb on + strand, within Psyr_3982 at 4735.194 kb on - strand, within Psyr_3982 at 4735.418 kb on + strand, within Psyr_3982 at 4735.419 kb on - strand, within Psyr_3982 at 4735.419 kb on - strand, within Psyr_3982 at 4735.461 kb on - strand, within Psyr_3982 at 4735.508 kb on - strand, within Psyr_3982 at 4735.537 kb on + strand, within Psyr_3982 at 4735.537 kb on + strand, within Psyr_3982 at 4735.537 kb on + strand, within Psyr_3982 at 4735.562 kb on + strand, within Psyr_3982 at 4735.562 kb on + strand, within Psyr_3982 at 4735.578 kb on - strand, within Psyr_3982 at 4735.616 kb on + strand, within Psyr_3982 at 4735.616 kb on + strand, within Psyr_3982 at 4735.616 kb on + strand, within Psyr_3982 at 4735.617 kb on - strand, within Psyr_3982 at 4735.617 kb on - strand, within Psyr_3982 at 4735.617 kb on - strand, within Psyr_3982 at 4735.617 kb on - strand, within Psyr_3982 at 4735.617 kb on - strand, within Psyr_3982 at 4735.617 kb on - strand, within Psyr_3982 at 4735.679 kb on + strand, within Psyr_3982 at 4735.679 kb on + strand, within Psyr_3982 at 4735.679 kb on + strand, within Psyr_3982 at 4735.679 kb on + strand, within Psyr_3982 at 4735.679 kb on + strand, within Psyr_3982 at 4735.680 kb on - strand, within Psyr_3982 at 4735.680 kb on - strand, within Psyr_3982 at 4735.680 kb on - strand, within Psyr_3982 at 4735.680 kb on - strand, within Psyr_3982 at 4735.680 kb on - strand, within Psyr_3982 at 4735.680 kb on - strand, within Psyr_3982 at 4736.015 kb on + strand at 4736.055 kb on - strand, within Psyr_RNA61 at 4736.055 kb on - strand, within Psyr_RNA61 at 4736.055 kb on - strand, within Psyr_RNA61 at 4736.055 kb on - strand, within Psyr_RNA61 at 4736.059 kb on + strand, within Psyr_RNA61 at 4736.066 kb on - strand, within Psyr_RNA61 at 4736.088 kb on + strand, within Psyr_RNA61 at 4736.089 kb on - strand, within Psyr_RNA61 at 4736.182 kb on + strand at 4736.182 kb on + strand at 4736.297 kb on - strand at 4736.297 kb on - strand at 4736.304 kb on + strand at 4736.356 kb on + strand at 4736.356 kb on + strand at 4736.357 kb on - strand at 4736.411 kb on + strand at 4736.411 kb on + strand at 4736.664 kb on - strand at 4736.685 kb on + strand at 4736.686 kb on - strand at 4736.686 kb on - strand at 4736.686 kb on - strand at 4736.686 kb on - strand at 4736.830 kb on - strand, within Psyr_3983 at 4736.830 kb on - strand, within Psyr_3983 at 4736.831 kb on + strand, within Psyr_3983 at 4736.831 kb on + strand, within Psyr_3983 at 4736.831 kb on + strand, within Psyr_3983 at 4736.832 kb on - strand, within Psyr_3983 at 4736.832 kb on - strand, within Psyr_3983 at 4737.009 kb on + strand, within Psyr_3983 at 4737.009 kb on + strand, within Psyr_3983 at 4737.010 kb on - strand, within Psyr_3983 at 4737.010 kb on - strand, within Psyr_3983 at 4737.010 kb on - strand, within Psyr_3983
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 4,735,055 + +1.7 4,735,056 - -0.9 4,735,181 + Psyr_3982 0.20 +3.4 4,735,194 - Psyr_3982 0.21 -2.9 4,735,418 + Psyr_3982 0.47 -2.2 4,735,419 - Psyr_3982 0.47 +2.1 4,735,419 - Psyr_3982 0.47 +0.6 4,735,461 - Psyr_3982 0.51 +0.1 4,735,508 - Psyr_3982 0.57 -1.5 4,735,537 + Psyr_3982 0.60 -0.3 4,735,537 + Psyr_3982 0.60 -0.6 4,735,537 + Psyr_3982 0.60 +3.2 4,735,562 + Psyr_3982 0.63 +2.1 4,735,562 + Psyr_3982 0.63 -2.1 4,735,578 - Psyr_3982 0.65 -1.8 4,735,616 + Psyr_3982 0.69 -1.3 4,735,616 + Psyr_3982 0.69 +3.4 4,735,616 + Psyr_3982 0.69 +1.1 4,735,617 - Psyr_3982 0.69 +2.1 4,735,617 - Psyr_3982 0.69 +1.1 4,735,617 - Psyr_3982 0.69 +0.1 4,735,617 - Psyr_3982 0.69 +4.1 4,735,617 - Psyr_3982 0.69 -0.0 4,735,617 - Psyr_3982 0.69 +0.5 4,735,679 + Psyr_3982 0.76 -1.9 4,735,679 + Psyr_3982 0.76 +0.3 4,735,679 + Psyr_3982 0.76 +3.7 4,735,679 + Psyr_3982 0.76 -1.1 4,735,679 + Psyr_3982 0.76 +0.9 4,735,680 - Psyr_3982 0.76 +2.1 4,735,680 - Psyr_3982 0.76 -1.8 4,735,680 - Psyr_3982 0.76 -2.5 4,735,680 - Psyr_3982 0.76 -0.7 4,735,680 - Psyr_3982 0.76 -0.3 4,735,680 - Psyr_3982 0.76 +3.7 4,736,015 + -3.2 4,736,055 - Psyr_RNA61 0.11 -1.2 4,736,055 - Psyr_RNA61 0.11 +3.1 4,736,055 - Psyr_RNA61 0.11 +0.7 4,736,055 - Psyr_RNA61 0.11 +3.2 4,736,059 + Psyr_RNA61 0.16 +3.2 4,736,066 - Psyr_RNA61 0.26 +0.1 4,736,088 + Psyr_RNA61 0.55 +2.1 4,736,089 - Psyr_RNA61 0.57 +0.1 4,736,182 + +0.4 4,736,182 + -0.8 4,736,297 - +1.1 4,736,297 - -0.6 4,736,304 + -3.0 4,736,356 + -2.0 4,736,356 + -1.2 4,736,357 - -2.6 4,736,411 + +2.7 4,736,411 + -0.9 4,736,664 - -1.5 4,736,685 + -2.7 4,736,686 - +3.1 4,736,686 - +0.8 4,736,686 - +4.2 4,736,686 - -2.7 4,736,830 - Psyr_3983 0.15 +0.1 4,736,830 - Psyr_3983 0.15 -1.6 4,736,831 + Psyr_3983 0.15 -1.3 4,736,831 + Psyr_3983 0.15 -0.9 4,736,831 + Psyr_3983 0.15 -1.9 4,736,832 - Psyr_3983 0.15 +2.1 4,736,832 - Psyr_3983 0.15 +3.2 4,737,009 + Psyr_3983 0.22 +1.1 4,737,009 + Psyr_3983 0.22 +1.7 4,737,010 - Psyr_3983 0.22 -2.6 4,737,010 - Psyr_3983 0.22 -2.4 4,737,010 - Psyr_3983 0.22 +0.1
Or see this region's nucleotide sequence