Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_5026 and Psyr_5027 are separated by 247 nucleotides Psyr_5027 and Psyr_5028 are separated by 192 nucleotides Psyr_5028 and Psyr_5029 are separated by 63 nucleotides Psyr_5029 and Psyr_5030 are separated by 189 nucleotides Psyr_5030 and Psyr_5031 overlap by 1 nucleotides
Psyr_5026: Psyr_5026 - conserved hypothetical protein, at 5,966,676 to 5,967,119
_5026
Psyr_5027: Psyr_5027 - bacterial nucleoid protein HU alpha subunit, at 5,967,367 to 5,967,642
_5027
Psyr_5028: Psyr_5028 - FAD-dependent pyridine nucleotide-disulfide oxidoreductase, at 5,967,835 to 5,968,983
_5028
Psyr_5029: Psyr_5029 - Rubredoxin-type Fe(Cys)4 protein, at 5,969,047 to 5,969,214
_5029
Psyr_5030: Psyr_5030 - chorismate lyase, at 5,969,404 to 5,969,964
_5030
Psyr_5031: Psyr_5031 - 4-hydroxybenzoate octaprenyltransferase, at 5,969,964 to 5,970,854
_5031
Position (kb) 5967
5968
5969 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 5966.890 kb on + strand, within Psyr_5026 at 5966.890 kb on + strand, within Psyr_5026 at 5966.890 kb on + strand, within Psyr_5026 at 5966.891 kb on - strand, within Psyr_5026 at 5966.891 kb on - strand, within Psyr_5026 at 5966.891 kb on - strand, within Psyr_5026 at 5966.891 kb on - strand, within Psyr_5026 at 5966.891 kb on - strand, within Psyr_5026 at 5966.891 kb on - strand, within Psyr_5026 at 5966.891 kb on - strand, within Psyr_5026 at 5967.052 kb on + strand, within Psyr_5026 at 5967.052 kb on + strand, within Psyr_5026 at 5967.052 kb on + strand, within Psyr_5026 at 5967.052 kb on + strand, within Psyr_5026 at 5967.053 kb on - strand, within Psyr_5026 at 5967.053 kb on - strand, within Psyr_5026 at 5967.053 kb on - strand, within Psyr_5026 at 5967.053 kb on - strand, within Psyr_5026 at 5967.053 kb on - strand, within Psyr_5026 at 5967.053 kb on - strand, within Psyr_5026 at 5967.053 kb on - strand, within Psyr_5026 at 5967.137 kb on + strand at 5967.137 kb on + strand at 5967.138 kb on - strand at 5967.138 kb on - strand at 5967.138 kb on - strand at 5967.138 kb on - strand at 5967.169 kb on - strand at 5967.169 kb on - strand at 5967.223 kb on + strand at 5967.223 kb on + strand at 5967.223 kb on + strand at 5967.223 kb on + strand at 5967.223 kb on + strand at 5967.223 kb on + strand at 5967.224 kb on - strand at 5967.224 kb on - strand at 5967.224 kb on - strand at 5967.224 kb on - strand at 5967.279 kb on + strand at 5967.279 kb on + strand at 5967.279 kb on + strand at 5967.280 kb on - strand at 5967.280 kb on - strand at 5967.508 kb on + strand, within Psyr_5027 at 5967.547 kb on - strand, within Psyr_5027 at 5967.642 kb on + strand at 5967.642 kb on + strand at 5967.642 kb on + strand at 5967.643 kb on - strand at 5967.643 kb on - strand at 5967.643 kb on - strand at 5967.657 kb on - strand at 5967.672 kb on + strand at 5967.672 kb on + strand at 5967.672 kb on + strand at 5967.672 kb on + strand at 5967.672 kb on + strand at 5967.673 kb on - strand at 5967.725 kb on + strand at 5967.726 kb on - strand at 5967.726 kb on - strand at 5967.726 kb on - strand at 5967.804 kb on + strand at 5967.804 kb on + strand at 5967.805 kb on - strand at 5968.055 kb on + strand, within Psyr_5028 at 5968.056 kb on - strand, within Psyr_5028 at 5968.127 kb on + strand, within Psyr_5028 at 5968.127 kb on + strand, within Psyr_5028 at 5968.127 kb on + strand, within Psyr_5028 at 5968.127 kb on + strand, within Psyr_5028 at 5968.127 kb on + strand, within Psyr_5028 at 5968.128 kb on - strand, within Psyr_5028 at 5968.128 kb on - strand, within Psyr_5028 at 5968.128 kb on - strand, within Psyr_5028 at 5968.586 kb on + strand, within Psyr_5028 at 5968.587 kb on - strand, within Psyr_5028 at 5968.688 kb on + strand, within Psyr_5028 at 5968.688 kb on + strand, within Psyr_5028 at 5968.688 kb on + strand, within Psyr_5028 at 5968.688 kb on + strand, within Psyr_5028 at 5968.688 kb on + strand, within Psyr_5028 at 5968.688 kb on + strand, within Psyr_5028 at 5968.688 kb on + strand, within Psyr_5028 at 5968.688 kb on + strand, within Psyr_5028 at 5968.689 kb on - strand, within Psyr_5028 at 5968.689 kb on - strand, within Psyr_5028 at 5968.689 kb on - strand, within Psyr_5028 at 5968.689 kb on - strand, within Psyr_5028 at 5968.689 kb on - strand, within Psyr_5028 at 5968.689 kb on - strand, within Psyr_5028 at 5968.689 kb on - strand, within Psyr_5028 at 5968.689 kb on - strand, within Psyr_5028 at 5968.689 kb on - strand, within Psyr_5028 at 5968.689 kb on - strand, within Psyr_5028 at 5968.862 kb on + strand, within Psyr_5028 at 5968.863 kb on - strand, within Psyr_5028 at 5968.863 kb on - strand, within Psyr_5028 at 5968.891 kb on + strand at 5968.892 kb on - strand at 5968.938 kb on + strand at 5968.938 kb on + strand at 5968.939 kb on - strand at 5968.940 kb on + strand at 5968.941 kb on - strand at 5968.994 kb on + strand at 5969.177 kb on + strand, within Psyr_5029 at 5969.177 kb on + strand, within Psyr_5029 at 5969.178 kb on - strand, within Psyr_5029 at 5969.178 kb on - strand, within Psyr_5029 at 5969.178 kb on - strand, within Psyr_5029 at 5969.231 kb on + strand at 5969.244 kb on - strand at 5969.248 kb on + strand at 5969.284 kb on + strand at 5969.284 kb on + strand at 5969.284 kb on + strand at 5969.285 kb on - strand at 5969.285 kb on - strand at 5969.285 kb on - strand at 5969.318 kb on + strand at 5969.318 kb on + strand at 5969.318 kb on + strand at 5969.318 kb on + strand at 5969.319 kb on - strand at 5969.365 kb on + strand at 5969.365 kb on + strand at 5969.400 kb on + strand at 5969.650 kb on + strand, within Psyr_5030 at 5969.650 kb on + strand, within Psyr_5030 at 5969.650 kb on + strand, within Psyr_5030 at 5969.650 kb on + strand, within Psyr_5030 at 5969.650 kb on + strand, within Psyr_5030 at 5969.650 kb on + strand, within Psyr_5030 at 5969.651 kb on - strand, within Psyr_5030 at 5969.967 kb on + strand at 5969.967 kb on + strand at 5969.967 kb on + strand at 5969.967 kb on + strand at 5969.967 kb on + strand at 5969.967 kb on + strand at 5969.967 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 5,966,890 + Psyr_5026 0.48 +3.3 5,966,890 + Psyr_5026 0.48 +4.0 5,966,890 + Psyr_5026 0.48 -0.2 5,966,891 - Psyr_5026 0.48 +2.6 5,966,891 - Psyr_5026 0.48 -1.5 5,966,891 - Psyr_5026 0.48 -0.0 5,966,891 - Psyr_5026 0.48 +2.9 5,966,891 - Psyr_5026 0.48 +4.5 5,966,891 - Psyr_5026 0.48 +4.0 5,966,891 - Psyr_5026 0.48 +3.3 5,967,052 + Psyr_5026 0.85 +2.0 5,967,052 + Psyr_5026 0.85 +3.6 5,967,052 + Psyr_5026 0.85 -0.8 5,967,052 + Psyr_5026 0.85 -0.5 5,967,053 - Psyr_5026 0.85 +0.9 5,967,053 - Psyr_5026 0.85 +2.3 5,967,053 - Psyr_5026 0.85 +3.5 5,967,053 - Psyr_5026 0.85 -0.4 5,967,053 - Psyr_5026 0.85 -2.6 5,967,053 - Psyr_5026 0.85 -1.3 5,967,053 - Psyr_5026 0.85 +2.6 5,967,137 + -0.1 5,967,137 + -1.2 5,967,138 - +2.3 5,967,138 - -2.8 5,967,138 - -2.2 5,967,138 - -0.3 5,967,169 - -2.1 5,967,169 - +3.6 5,967,223 + +1.7 5,967,223 + -2.9 5,967,223 + +2.3 5,967,223 + -0.7 5,967,223 + -1.3 5,967,223 + +3.9 5,967,224 - -1.6 5,967,224 - -2.9 5,967,224 - +2.9 5,967,224 - -4.7 5,967,279 + +3.3 5,967,279 + -2.0 5,967,279 + +0.3 5,967,280 - -3.0 5,967,280 - -1.3 5,967,508 + Psyr_5027 0.51 +4.2 5,967,547 - Psyr_5027 0.65 -1.7 5,967,642 + -0.0 5,967,642 + -2.5 5,967,642 + -2.4 5,967,643 - -1.4 5,967,643 - -1.5 5,967,643 - -1.0 5,967,657 - -1.2 5,967,672 + +1.3 5,967,672 + +0.4 5,967,672 + -0.4 5,967,672 + +3.5 5,967,672 + -0.5 5,967,673 - -0.0 5,967,725 + +1.9 5,967,726 - -0.3 5,967,726 - +0.6 5,967,726 - -0.1 5,967,804 + -0.2 5,967,804 + +3.6 5,967,805 - -1.2 5,968,055 + Psyr_5028 0.19 -0.2 5,968,056 - Psyr_5028 0.19 +3.7 5,968,127 + Psyr_5028 0.25 +1.8 5,968,127 + Psyr_5028 0.25 +4.5 5,968,127 + Psyr_5028 0.25 +3.9 5,968,127 + Psyr_5028 0.25 +1.9 5,968,127 + Psyr_5028 0.25 +1.7 5,968,128 - Psyr_5028 0.26 +2.3 5,968,128 - Psyr_5028 0.26 -0.5 5,968,128 - Psyr_5028 0.26 -0.0 5,968,586 + Psyr_5028 0.65 +0.1 5,968,587 - Psyr_5028 0.65 -0.3 5,968,688 + Psyr_5028 0.74 +0.5 5,968,688 + Psyr_5028 0.74 -0.7 5,968,688 + Psyr_5028 0.74 -0.5 5,968,688 + Psyr_5028 0.74 +0.0 5,968,688 + Psyr_5028 0.74 -2.7 5,968,688 + Psyr_5028 0.74 +0.8 5,968,688 + Psyr_5028 0.74 -1.3 5,968,688 + Psyr_5028 0.74 +0.2 5,968,689 - Psyr_5028 0.74 +0.8 5,968,689 - Psyr_5028 0.74 -1.8 5,968,689 - Psyr_5028 0.74 -2.5 5,968,689 - Psyr_5028 0.74 +2.3 5,968,689 - Psyr_5028 0.74 -4.3 5,968,689 - Psyr_5028 0.74 +2.6 5,968,689 - Psyr_5028 0.74 -0.1 5,968,689 - Psyr_5028 0.74 +1.0 5,968,689 - Psyr_5028 0.74 -0.1 5,968,689 - Psyr_5028 0.74 +1.9 5,968,862 + Psyr_5028 0.89 -2.4 5,968,863 - Psyr_5028 0.89 +3.7 5,968,863 - Psyr_5028 0.89 +0.5 5,968,891 + -0.4 5,968,892 - -0.0 5,968,938 + -0.5 5,968,938 + -0.6 5,968,939 - +0.5 5,968,940 + -1.4 5,968,941 - -0.5 5,968,994 + +3.5 5,969,177 + Psyr_5029 0.77 +3.7 5,969,177 + Psyr_5029 0.77 -2.3 5,969,178 - Psyr_5029 0.78 +3.9 5,969,178 - Psyr_5029 0.78 -1.6 5,969,178 - Psyr_5029 0.78 +2.3 5,969,231 + -0.3 5,969,244 - -0.1 5,969,248 + +0.3 5,969,284 + -1.5 5,969,284 + -2.0 5,969,284 + +3.1 5,969,285 - +4.3 5,969,285 - -0.5 5,969,285 - +0.4 5,969,318 + -1.0 5,969,318 + -1.2 5,969,318 + -2.6 5,969,318 + -1.6 5,969,319 - +0.3 5,969,365 + +1.2 5,969,365 + +2.9 5,969,400 + -0.2 5,969,650 + Psyr_5030 0.44 +0.2 5,969,650 + Psyr_5030 0.44 +3.3 5,969,650 + Psyr_5030 0.44 +1.5 5,969,650 + Psyr_5030 0.44 -1.3 5,969,650 + Psyr_5030 0.44 +1.3 5,969,650 + Psyr_5030 0.44 +3.7 5,969,651 - Psyr_5030 0.44 -0.9 5,969,967 + +5.1 5,969,967 + -0.7 5,969,967 + -1.8 5,969,967 + +0.5 5,969,967 + -1.8 5,969,967 + -1.9 5,969,967 + -1.0
Or see this region's nucleotide sequence