Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4941 and Psyr_4942 are separated by 97 nucleotides Psyr_4942 and Psyr_4943 are separated by 44 nucleotides Psyr_4943 and Psyr_4944 are separated by 149 nucleotides
Psyr_4941: Psyr_4941 - NUDIX hydrolase, at 5,857,194 to 5,857,760
_4941
Psyr_4942: Psyr_4942 - HAD-superfamily hydrolase, subfamily IA, variant 3, at 5,857,858 to 5,858,520
_4942
Psyr_4943: Psyr_4943 - Peptidoglycan-binding LysM:Transport-associated, at 5,858,565 to 5,859,005
_4943
Psyr_4944: Psyr_4944 - transcriptional regulator, LysR family, at 5,859,155 to 5,860,036
_4944
Position (kb) 5858
5859
5860 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 5857.654 kb on + strand, within Psyr_4941 at 5857.655 kb on - strand, within Psyr_4941 at 5857.655 kb on - strand, within Psyr_4941 at 5857.655 kb on - strand, within Psyr_4941 at 5857.655 kb on - strand, within Psyr_4941 at 5857.655 kb on - strand, within Psyr_4941 at 5857.740 kb on + strand at 5857.740 kb on + strand at 5857.740 kb on + strand at 5857.740 kb on + strand at 5857.740 kb on + strand at 5857.740 kb on + strand at 5857.740 kb on + strand at 5857.740 kb on + strand at 5857.741 kb on - strand at 5857.741 kb on - strand at 5857.741 kb on - strand at 5857.741 kb on - strand at 5857.741 kb on - strand at 5857.741 kb on - strand at 5857.741 kb on - strand at 5857.741 kb on - strand at 5857.745 kb on + strand at 5857.746 kb on - strand at 5857.746 kb on - strand at 5857.746 kb on - strand at 5857.746 kb on - strand at 5857.746 kb on - strand at 5857.773 kb on + strand at 5857.773 kb on + strand at 5857.773 kb on + strand at 5857.773 kb on + strand at 5857.773 kb on + strand at 5857.773 kb on + strand at 5857.773 kb on + strand at 5857.774 kb on - strand at 5857.774 kb on - strand at 5857.774 kb on - strand at 5857.774 kb on - strand at 5857.774 kb on - strand at 5857.774 kb on - strand at 5857.774 kb on - strand at 5857.786 kb on + strand at 5857.786 kb on + strand at 5857.786 kb on + strand at 5857.786 kb on + strand at 5857.786 kb on + strand at 5857.786 kb on + strand at 5857.786 kb on + strand at 5857.786 kb on + strand at 5857.787 kb on - strand at 5857.787 kb on - strand at 5857.789 kb on + strand at 5857.789 kb on + strand at 5857.789 kb on + strand at 5857.789 kb on + strand at 5857.789 kb on + strand at 5857.789 kb on + strand at 5857.790 kb on - strand at 5857.790 kb on - strand at 5857.790 kb on - strand at 5857.790 kb on - strand at 5857.790 kb on - strand at 5857.790 kb on - strand at 5857.790 kb on - strand at 5857.790 kb on - strand at 5857.790 kb on - strand at 5857.790 kb on - strand at 5857.790 kb on - strand at 5857.790 kb on - strand at 5857.790 kb on - strand at 5857.790 kb on - strand at 5857.790 kb on - strand at 5857.790 kb on - strand at 5857.790 kb on - strand at 5857.790 kb on - strand at 5857.790 kb on - strand at 5857.791 kb on + strand at 5857.791 kb on + strand at 5857.791 kb on + strand at 5857.792 kb on - strand at 5857.792 kb on - strand at 5857.792 kb on - strand at 5857.792 kb on - strand at 5857.931 kb on - strand, within Psyr_4942 at 5857.931 kb on - strand, within Psyr_4942 at 5858.057 kb on - strand, within Psyr_4942 at 5858.257 kb on + strand, within Psyr_4942 at 5858.257 kb on + strand, within Psyr_4942 at 5858.414 kb on + strand, within Psyr_4942 at 5858.414 kb on + strand, within Psyr_4942 at 5858.415 kb on - strand, within Psyr_4942 at 5858.518 kb on + strand at 5858.518 kb on + strand at 5858.518 kb on + strand at 5858.519 kb on - strand at 5858.519 kb on - strand at 5858.519 kb on - strand at 5858.519 kb on - strand at 5858.519 kb on - strand at 5858.651 kb on - strand, within Psyr_4943 at 5858.668 kb on + strand, within Psyr_4943 at 5858.668 kb on + strand, within Psyr_4943 at 5858.684 kb on + strand, within Psyr_4943 at 5858.685 kb on - strand, within Psyr_4943 at 5858.719 kb on + strand, within Psyr_4943 at 5858.720 kb on - strand, within Psyr_4943 at 5858.909 kb on + strand, within Psyr_4943 at 5858.909 kb on + strand, within Psyr_4943 at 5858.909 kb on + strand, within Psyr_4943 at 5858.909 kb on + strand, within Psyr_4943 at 5858.909 kb on + strand, within Psyr_4943 at 5858.909 kb on + strand, within Psyr_4943 at 5858.909 kb on + strand, within Psyr_4943 at 5858.910 kb on - strand, within Psyr_4943 at 5858.910 kb on - strand, within Psyr_4943 at 5858.910 kb on - strand, within Psyr_4943 at 5858.996 kb on + strand at 5858.997 kb on - strand at 5859.020 kb on + strand at 5859.020 kb on + strand at 5859.021 kb on - strand at 5859.024 kb on + strand at 5859.024 kb on + strand at 5859.024 kb on + strand at 5859.024 kb on + strand at 5859.024 kb on + strand at 5859.024 kb on + strand at 5859.025 kb on - strand at 5859.025 kb on - strand at 5859.025 kb on - strand at 5859.025 kb on - strand at 5859.045 kb on + strand at 5859.046 kb on - strand at 5859.059 kb on + strand at 5859.090 kb on + strand at 5859.090 kb on + strand at 5859.090 kb on + strand at 5859.090 kb on + strand at 5859.090 kb on + strand at 5859.091 kb on - strand at 5859.091 kb on - strand at 5859.091 kb on - strand at 5859.091 kb on - strand at 5859.091 kb on - strand at 5859.091 kb on - strand at 5859.119 kb on + strand at 5859.120 kb on - strand at 5859.155 kb on - strand at 5859.173 kb on - strand at 5859.173 kb on - strand at 5859.232 kb on + strand at 5859.232 kb on + strand at 5859.232 kb on + strand at 5859.233 kb on - strand at 5859.233 kb on - strand at 5859.233 kb on - strand at 5859.235 kb on + strand at 5859.571 kb on + strand, within Psyr_4944 at 5859.590 kb on + strand, within Psyr_4944 at 5859.659 kb on + strand, within Psyr_4944 at 5859.659 kb on + strand, within Psyr_4944 at 5859.659 kb on + strand, within Psyr_4944 at 5859.659 kb on + strand, within Psyr_4944 at 5859.659 kb on + strand, within Psyr_4944 at 5859.660 kb on - strand, within Psyr_4944 at 5859.660 kb on - strand, within Psyr_4944 at 5859.660 kb on - strand, within Psyr_4944 at 5859.676 kb on + strand, within Psyr_4944 at 5859.676 kb on + strand, within Psyr_4944 at 5859.677 kb on - strand, within Psyr_4944 at 5859.810 kb on + strand, within Psyr_4944 at 5859.847 kb on - strand, within Psyr_4944
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 5,857,654 + Psyr_4941 0.81 +2.8 5,857,655 - Psyr_4941 0.81 +1.8 5,857,655 - Psyr_4941 0.81 -0.9 5,857,655 - Psyr_4941 0.81 -1.2 5,857,655 - Psyr_4941 0.81 +0.7 5,857,655 - Psyr_4941 0.81 -2.9 5,857,740 + -0.0 5,857,740 + +1.2 5,857,740 + -2.5 5,857,740 + +0.3 5,857,740 + +3.6 5,857,740 + -0.2 5,857,740 + -2.0 5,857,740 + -1.6 5,857,741 - -0.7 5,857,741 - -1.2 5,857,741 - -0.5 5,857,741 - +2.6 5,857,741 - -0.9 5,857,741 - -2.1 5,857,741 - +0.3 5,857,741 - -0.7 5,857,745 + +2.8 5,857,746 - +1.0 5,857,746 - -2.1 5,857,746 - -0.5 5,857,746 - +4.3 5,857,746 - -1.8 5,857,773 + -0.3 5,857,773 + -0.6 5,857,773 + +3.4 5,857,773 + -0.3 5,857,773 + -0.7 5,857,773 + +0.0 5,857,773 + +0.3 5,857,774 - +0.5 5,857,774 - +1.8 5,857,774 - +0.1 5,857,774 - +3.8 5,857,774 - -0.3 5,857,774 - -0.5 5,857,774 - +2.3 5,857,786 + -1.7 5,857,786 + +0.0 5,857,786 + +0.3 5,857,786 + +1.3 5,857,786 + +0.8 5,857,786 + +1.0 5,857,786 + -4.7 5,857,786 + -0.3 5,857,787 - +2.2 5,857,787 - +2.3 5,857,789 + +3.4 5,857,789 + -1.0 5,857,789 + -1.2 5,857,789 + -0.8 5,857,789 + -1.1 5,857,789 + -0.5 5,857,790 - +3.3 5,857,790 - +2.8 5,857,790 - -0.2 5,857,790 - +1.3 5,857,790 - +1.4 5,857,790 - +0.6 5,857,790 - +2.3 5,857,790 - +0.8 5,857,790 - +2.8 5,857,790 - -1.5 5,857,790 - +4.5 5,857,790 - +2.8 5,857,790 - +4.2 5,857,790 - -0.4 5,857,790 - +4.4 5,857,790 - +3.3 5,857,790 - +3.8 5,857,790 - +1.3 5,857,790 - -0.4 5,857,791 + +2.8 5,857,791 + +3.4 5,857,791 + -0.5 5,857,792 - +3.4 5,857,792 - +0.3 5,857,792 - +0.5 5,857,792 - -1.6 5,857,931 - Psyr_4942 0.11 +4.0 5,857,931 - Psyr_4942 0.11 -1.9 5,858,057 - Psyr_4942 0.30 +0.5 5,858,257 + Psyr_4942 0.60 -0.7 5,858,257 + Psyr_4942 0.60 -1.1 5,858,414 + Psyr_4942 0.84 +3.3 5,858,414 + Psyr_4942 0.84 +2.3 5,858,415 - Psyr_4942 0.84 +0.7 5,858,518 + -0.3 5,858,518 + +3.1 5,858,518 + +3.6 5,858,519 - -0.5 5,858,519 - +1.8 5,858,519 - +3.1 5,858,519 - +0.1 5,858,519 - -0.7 5,858,651 - Psyr_4943 0.20 -1.8 5,858,668 + Psyr_4943 0.23 +3.7 5,858,668 + Psyr_4943 0.23 -3.6 5,858,684 + Psyr_4943 0.27 -1.0 5,858,685 - Psyr_4943 0.27 -1.8 5,858,719 + Psyr_4943 0.35 +1.3 5,858,720 - Psyr_4943 0.35 -1.8 5,858,909 + Psyr_4943 0.78 -0.1 5,858,909 + Psyr_4943 0.78 -1.3 5,858,909 + Psyr_4943 0.78 -0.5 5,858,909 + Psyr_4943 0.78 -1.6 5,858,909 + Psyr_4943 0.78 -1.2 5,858,909 + Psyr_4943 0.78 -0.4 5,858,909 + Psyr_4943 0.78 +3.4 5,858,910 - Psyr_4943 0.78 +1.7 5,858,910 - Psyr_4943 0.78 -1.7 5,858,910 - Psyr_4943 0.78 -2.7 5,858,996 + +3.7 5,858,997 - +3.6 5,859,020 + -0.7 5,859,020 + -1.2 5,859,021 - +2.8 5,859,024 + +2.3 5,859,024 + +3.7 5,859,024 + +4.4 5,859,024 + +2.8 5,859,024 + -1.9 5,859,024 + +2.8 5,859,025 - +3.6 5,859,025 - -2.5 5,859,025 - +3.1 5,859,025 - -0.2 5,859,045 + -0.1 5,859,046 - -2.2 5,859,059 + -1.5 5,859,090 + +2.6 5,859,090 + +1.3 5,859,090 + +3.1 5,859,090 + +0.3 5,859,090 + +0.2 5,859,091 - -2.8 5,859,091 - +2.3 5,859,091 - -2.5 5,859,091 - +0.2 5,859,091 - -0.7 5,859,091 - -1.7 5,859,119 + +1.8 5,859,120 - -0.1 5,859,155 - +3.6 5,859,173 - -0.2 5,859,173 - +0.6 5,859,232 + +1.9 5,859,232 + +0.0 5,859,232 + +4.2 5,859,233 - -0.7 5,859,233 - -0.9 5,859,233 - -0.8 5,859,235 + -0.1 5,859,571 + Psyr_4944 0.47 +1.0 5,859,590 + Psyr_4944 0.49 -0.7 5,859,659 + Psyr_4944 0.57 +1.5 5,859,659 + Psyr_4944 0.57 +0.7 5,859,659 + Psyr_4944 0.57 -1.3 5,859,659 + Psyr_4944 0.57 +0.3 5,859,659 + Psyr_4944 0.57 +4.0 5,859,660 - Psyr_4944 0.57 +0.3 5,859,660 - Psyr_4944 0.57 -1.3 5,859,660 - Psyr_4944 0.57 -0.5 5,859,676 + Psyr_4944 0.59 -0.8 5,859,676 + Psyr_4944 0.59 -4.2 5,859,677 - Psyr_4944 0.59 -1.4 5,859,810 + Psyr_4944 0.74 -0.1 5,859,847 - Psyr_4944 0.78 +1.0
Or see this region's nucleotide sequence