Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4927 and Psyr_4928 are separated by 43 nucleotides Psyr_4928 and Psyr_4929 are separated by 166 nucleotides Psyr_4929 and Psyr_4930 are separated by 271 nucleotides
Psyr_4927: Psyr_4927 - ATP-dependent DNA helicase recG, at 5,840,727 to 5,841,323
_4927
Psyr_4928: Psyr_4928 - conserved hypothetical protein, at 5,841,367 to 5,841,954
_4928
Psyr_4929: Psyr_4929 - conserved hypothetical protein, at 5,842,121 to 5,842,591
_4929
Psyr_4930: Psyr_4930 - outer membrane porin, at 5,842,863 to 5,844,137
_4930
Position (kb) 5842
5843 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 5841.124 kb on + strand, within Psyr_4927 at 5841.124 kb on + strand, within Psyr_4927 at 5841.164 kb on + strand, within Psyr_4927 at 5841.164 kb on + strand, within Psyr_4927 at 5841.164 kb on + strand, within Psyr_4927 at 5841.164 kb on + strand, within Psyr_4927 at 5841.164 kb on + strand, within Psyr_4927 at 5841.165 kb on - strand, within Psyr_4927 at 5841.165 kb on - strand, within Psyr_4927 at 5841.165 kb on - strand, within Psyr_4927 at 5841.249 kb on + strand, within Psyr_4927 at 5841.249 kb on + strand, within Psyr_4927 at 5841.250 kb on - strand, within Psyr_4927 at 5841.250 kb on - strand, within Psyr_4927 at 5841.250 kb on - strand, within Psyr_4927 at 5841.250 kb on - strand, within Psyr_4927 at 5841.277 kb on + strand at 5841.277 kb on + strand at 5841.278 kb on - strand at 5841.278 kb on - strand at 5841.315 kb on + strand at 5841.315 kb on + strand at 5841.315 kb on + strand at 5841.315 kb on + strand at 5841.315 kb on + strand at 5841.315 kb on + strand at 5841.316 kb on - strand at 5841.316 kb on - strand at 5841.316 kb on - strand at 5841.316 kb on - strand at 5841.316 kb on - strand at 5841.316 kb on - strand at 5841.317 kb on + strand at 5841.332 kb on - strand at 5841.339 kb on + strand at 5841.339 kb on + strand at 5841.339 kb on + strand at 5841.340 kb on - strand at 5841.340 kb on - strand at 5841.340 kb on - strand at 5841.340 kb on - strand at 5841.340 kb on - strand at 5841.368 kb on + strand at 5841.611 kb on + strand, within Psyr_4928 at 5841.748 kb on + strand, within Psyr_4928 at 5841.749 kb on - strand, within Psyr_4928 at 5841.776 kb on + strand, within Psyr_4928 at 5841.776 kb on + strand, within Psyr_4928 at 5841.777 kb on - strand, within Psyr_4928 at 5841.777 kb on - strand, within Psyr_4928 at 5841.777 kb on - strand, within Psyr_4928 at 5841.828 kb on - strand, within Psyr_4928 at 5841.985 kb on + strand at 5841.985 kb on + strand at 5841.985 kb on + strand at 5841.998 kb on + strand at 5841.999 kb on - strand at 5842.043 kb on + strand at 5842.043 kb on + strand at 5842.044 kb on - strand at 5842.044 kb on - strand at 5842.044 kb on - strand at 5842.045 kb on + strand at 5842.045 kb on + strand at 5842.045 kb on + strand at 5842.046 kb on - strand at 5842.046 kb on - strand at 5842.046 kb on - strand at 5842.208 kb on + strand, within Psyr_4929 at 5842.208 kb on + strand, within Psyr_4929 at 5842.209 kb on - strand, within Psyr_4929 at 5842.209 kb on - strand, within Psyr_4929 at 5842.209 kb on - strand, within Psyr_4929 at 5842.236 kb on + strand, within Psyr_4929 at 5842.236 kb on + strand, within Psyr_4929 at 5842.236 kb on + strand, within Psyr_4929 at 5842.237 kb on - strand, within Psyr_4929 at 5842.241 kb on + strand, within Psyr_4929 at 5842.242 kb on - strand, within Psyr_4929 at 5842.242 kb on - strand, within Psyr_4929 at 5842.242 kb on - strand, within Psyr_4929 at 5842.242 kb on - strand, within Psyr_4929 at 5842.242 kb on - strand, within Psyr_4929 at 5842.242 kb on - strand, within Psyr_4929 at 5842.242 kb on - strand, within Psyr_4929 at 5842.288 kb on + strand, within Psyr_4929 at 5842.289 kb on - strand, within Psyr_4929 at 5842.333 kb on + strand, within Psyr_4929 at 5842.334 kb on - strand, within Psyr_4929 at 5842.334 kb on - strand, within Psyr_4929 at 5842.439 kb on + strand, within Psyr_4929 at 5842.439 kb on + strand, within Psyr_4929 at 5842.439 kb on + strand, within Psyr_4929 at 5842.439 kb on + strand, within Psyr_4929 at 5842.439 kb on + strand, within Psyr_4929 at 5842.439 kb on + strand, within Psyr_4929 at 5842.440 kb on - strand, within Psyr_4929 at 5842.440 kb on - strand, within Psyr_4929 at 5842.440 kb on - strand, within Psyr_4929 at 5842.440 kb on - strand, within Psyr_4929 at 5842.440 kb on - strand, within Psyr_4929 at 5842.440 kb on - strand, within Psyr_4929 at 5842.440 kb on - strand, within Psyr_4929 at 5842.645 kb on + strand at 5842.645 kb on + strand at 5842.646 kb on - strand at 5842.646 kb on - strand at 5842.646 kb on - strand at 5842.646 kb on - strand at 5842.673 kb on + strand at 5842.673 kb on + strand at 5842.673 kb on + strand at 5842.789 kb on + strand at 5842.789 kb on + strand at 5842.789 kb on + strand at 5842.789 kb on + strand at 5842.789 kb on + strand at 5842.789 kb on + strand at 5842.789 kb on + strand at 5842.789 kb on + strand at 5842.789 kb on + strand at 5842.789 kb on + strand at 5842.789 kb on + strand at 5842.789 kb on + strand at 5842.789 kb on + strand at 5842.791 kb on + strand at 5842.921 kb on + strand at 5842.921 kb on + strand at 5842.921 kb on + strand at 5842.922 kb on - strand at 5843.005 kb on + strand, within Psyr_4930 at 5843.005 kb on + strand, within Psyr_4930 at 5843.005 kb on + strand, within Psyr_4930 at 5843.005 kb on + strand, within Psyr_4930 at 5843.006 kb on - strand, within Psyr_4930 at 5843.006 kb on - strand, within Psyr_4930 at 5843.006 kb on - strand, within Psyr_4930 at 5843.006 kb on - strand, within Psyr_4930 at 5843.006 kb on - strand, within Psyr_4930 at 5843.022 kb on + strand, within Psyr_4930 at 5843.082 kb on + strand, within Psyr_4930 at 5843.082 kb on + strand, within Psyr_4930 at 5843.086 kb on + strand at 5843.086 kb on + strand, within Psyr_4930 at 5843.086 kb on + strand, within Psyr_4930 at 5843.087 kb on - strand, within Psyr_4930 at 5843.087 kb on - strand, within Psyr_4930 at 5843.087 kb on - strand, within Psyr_4930 at 5843.087 kb on - strand, within Psyr_4930 at 5843.087 kb on - strand, within Psyr_4930 at 5843.098 kb on + strand, within Psyr_4930 at 5843.098 kb on + strand, within Psyr_4930 at 5843.098 kb on + strand, within Psyr_4930 at 5843.098 kb on + strand, within Psyr_4930 at 5843.098 kb on + strand, within Psyr_4930 at 5843.098 kb on + strand, within Psyr_4930 at 5843.099 kb on - strand, within Psyr_4930 at 5843.099 kb on - strand, within Psyr_4930 at 5843.099 kb on - strand, within Psyr_4930 at 5843.102 kb on + strand, within Psyr_4930 at 5843.102 kb on + strand, within Psyr_4930 at 5843.102 kb on + strand, within Psyr_4930 at 5843.103 kb on - strand, within Psyr_4930 at 5843.103 kb on - strand, within Psyr_4930 at 5843.141 kb on - strand, within Psyr_4930 at 5843.188 kb on + strand, within Psyr_4930 at 5843.188 kb on + strand, within Psyr_4930 at 5843.203 kb on + strand, within Psyr_4930 at 5843.203 kb on + strand, within Psyr_4930 at 5843.203 kb on + strand, within Psyr_4930 at 5843.204 kb on - strand, within Psyr_4930 at 5843.204 kb on - strand, within Psyr_4930 at 5843.204 kb on - strand, within Psyr_4930 at 5843.204 kb on - strand, within Psyr_4930 at 5843.204 kb on - strand, within Psyr_4930 at 5843.204 kb on - strand, within Psyr_4930 at 5843.261 kb on - strand, within Psyr_4930 at 5843.446 kb on + strand, within Psyr_4930 at 5843.446 kb on + strand, within Psyr_4930 at 5843.446 kb on + strand, within Psyr_4930 at 5843.446 kb on + strand, within Psyr_4930 at 5843.446 kb on + strand, within Psyr_4930 at 5843.446 kb on + strand, within Psyr_4930 at 5843.446 kb on + strand, within Psyr_4930 at 5843.446 kb on + strand, within Psyr_4930 at 5843.446 kb on + strand, within Psyr_4930 at 5843.447 kb on - strand, within Psyr_4930 at 5843.447 kb on - strand, within Psyr_4930 at 5843.447 kb on - strand, within Psyr_4930 at 5843.447 kb on - strand, within Psyr_4930 at 5843.447 kb on - strand, within Psyr_4930 at 5843.447 kb on - strand, within Psyr_4930 at 5843.447 kb on - strand, within Psyr_4930 at 5843.447 kb on - strand, within Psyr_4930 at 5843.447 kb on - strand, within Psyr_4930 at 5843.447 kb on - strand, within Psyr_4930 at 5843.447 kb on - strand, within Psyr_4930 at 5843.447 kb on - strand, within Psyr_4930 at 5843.447 kb on - strand, within Psyr_4930 at 5843.447 kb on - strand, within Psyr_4930 at 5843.447 kb on - strand, within Psyr_4930 at 5843.462 kb on - strand, within Psyr_4930 at 5843.497 kb on + strand, within Psyr_4930 at 5843.497 kb on + strand, within Psyr_4930 at 5843.498 kb on - strand, within Psyr_4930 at 5843.498 kb on - strand, within Psyr_4930 at 5843.498 kb on - strand, within Psyr_4930 at 5843.498 kb on - strand, within Psyr_4930 at 5843.530 kb on + strand, within Psyr_4930
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 5,841,124 + Psyr_4927 0.66 -1.2 5,841,124 + Psyr_4927 0.66 -0.4 5,841,164 + Psyr_4927 0.73 -1.9 5,841,164 + Psyr_4927 0.73 -0.8 5,841,164 + Psyr_4927 0.73 +4.1 5,841,164 + Psyr_4927 0.73 +2.6 5,841,164 + Psyr_4927 0.73 -2.4 5,841,165 - Psyr_4927 0.73 +0.3 5,841,165 - Psyr_4927 0.73 +1.8 5,841,165 - Psyr_4927 0.73 +3.3 5,841,249 + Psyr_4927 0.87 +1.3 5,841,249 + Psyr_4927 0.87 -1.3 5,841,250 - Psyr_4927 0.88 +3.6 5,841,250 - Psyr_4927 0.88 +1.8 5,841,250 - Psyr_4927 0.88 -1.6 5,841,250 - Psyr_4927 0.88 +0.3 5,841,277 + +0.5 5,841,277 + +2.3 5,841,278 - -2.6 5,841,278 - +0.0 5,841,315 + -0.5 5,841,315 + +1.0 5,841,315 + +2.6 5,841,315 + -0.6 5,841,315 + -0.9 5,841,315 + -1.7 5,841,316 - +3.6 5,841,316 - +2.2 5,841,316 - -2.0 5,841,316 - -0.2 5,841,316 - -0.9 5,841,316 - -0.7 5,841,317 + -0.8 5,841,332 - -2.3 5,841,339 + -1.7 5,841,339 + -1.2 5,841,339 + -2.0 5,841,340 - -0.3 5,841,340 - -1.2 5,841,340 - +2.3 5,841,340 - -1.3 5,841,340 - +2.6 5,841,368 + -2.4 5,841,611 + Psyr_4928 0.41 +2.8 5,841,748 + Psyr_4928 0.65 +0.8 5,841,749 - Psyr_4928 0.65 -0.2 5,841,776 + Psyr_4928 0.70 +3.2 5,841,776 + Psyr_4928 0.70 +2.3 5,841,777 - Psyr_4928 0.70 +0.2 5,841,777 - Psyr_4928 0.70 +4.1 5,841,777 - Psyr_4928 0.70 -0.6 5,841,828 - Psyr_4928 0.78 -1.0 5,841,985 + -3.2 5,841,985 + -0.6 5,841,985 + +4.3 5,841,998 + -0.6 5,841,999 - -1.7 5,842,043 + +0.6 5,842,043 + +2.6 5,842,044 - +2.8 5,842,044 - -0.9 5,842,044 - -0.2 5,842,045 + +4.6 5,842,045 + -1.4 5,842,045 + -1.8 5,842,046 - -1.8 5,842,046 - -0.3 5,842,046 - +0.4 5,842,208 + Psyr_4929 0.18 +0.1 5,842,208 + Psyr_4929 0.18 +1.3 5,842,209 - Psyr_4929 0.19 +3.4 5,842,209 - Psyr_4929 0.19 +2.8 5,842,209 - Psyr_4929 0.19 -0.3 5,842,236 + Psyr_4929 0.24 -1.0 5,842,236 + Psyr_4929 0.24 -0.3 5,842,236 + Psyr_4929 0.24 -2.2 5,842,237 - Psyr_4929 0.25 +5.0 5,842,241 + Psyr_4929 0.25 -2.1 5,842,242 - Psyr_4929 0.26 -1.5 5,842,242 - Psyr_4929 0.26 -0.2 5,842,242 - Psyr_4929 0.26 +0.3 5,842,242 - Psyr_4929 0.26 +1.3 5,842,242 - Psyr_4929 0.26 -0.9 5,842,242 - Psyr_4929 0.26 +4.0 5,842,242 - Psyr_4929 0.26 -1.3 5,842,288 + Psyr_4929 0.35 -0.8 5,842,289 - Psyr_4929 0.36 -1.3 5,842,333 + Psyr_4929 0.45 -1.4 5,842,334 - Psyr_4929 0.45 -1.9 5,842,334 - Psyr_4929 0.45 -3.3 5,842,439 + Psyr_4929 0.68 -1.2 5,842,439 + Psyr_4929 0.68 -0.1 5,842,439 + Psyr_4929 0.68 -1.1 5,842,439 + Psyr_4929 0.68 +0.3 5,842,439 + Psyr_4929 0.68 +2.6 5,842,439 + Psyr_4929 0.68 -1.1 5,842,440 - Psyr_4929 0.68 -1.3 5,842,440 - Psyr_4929 0.68 -1.2 5,842,440 - Psyr_4929 0.68 +2.3 5,842,440 - Psyr_4929 0.68 +5.3 5,842,440 - Psyr_4929 0.68 +1.4 5,842,440 - Psyr_4929 0.68 +1.4 5,842,440 - Psyr_4929 0.68 -1.6 5,842,645 + -0.5 5,842,645 + -0.4 5,842,646 - -4.4 5,842,646 - +2.8 5,842,646 - +1.3 5,842,646 - -1.7 5,842,673 + +2.6 5,842,673 + -1.1 5,842,673 + +4.1 5,842,789 + +1.0 5,842,789 + +0.8 5,842,789 + -1.2 5,842,789 + +2.8 5,842,789 + +1.8 5,842,789 + +1.1 5,842,789 + +3.1 5,842,789 + +3.8 5,842,789 + +1.2 5,842,789 + +2.8 5,842,789 + -0.2 5,842,789 + -0.5 5,842,789 + -1.3 5,842,791 + -1.6 5,842,921 + +0.2 5,842,921 + -1.4 5,842,921 + +3.7 5,842,922 - -0.2 5,843,005 + Psyr_4930 0.11 -0.6 5,843,005 + Psyr_4930 0.11 +1.2 5,843,005 + Psyr_4930 0.11 -1.2 5,843,005 + Psyr_4930 0.11 +0.0 5,843,006 - Psyr_4930 0.11 +0.3 5,843,006 - Psyr_4930 0.11 -0.0 5,843,006 - Psyr_4930 0.11 +0.3 5,843,006 - Psyr_4930 0.11 +0.1 5,843,006 - Psyr_4930 0.11 -1.1 5,843,022 + Psyr_4930 0.12 -2.8 5,843,082 + Psyr_4930 0.17 +2.6 5,843,082 + Psyr_4930 0.17 +1.8 5,843,086 + +1.8 5,843,086 + Psyr_4930 0.17 -2.2 5,843,086 + Psyr_4930 0.17 +0.3 5,843,087 - Psyr_4930 0.18 -2.5 5,843,087 - Psyr_4930 0.18 -1.5 5,843,087 - Psyr_4930 0.18 -0.5 5,843,087 - Psyr_4930 0.18 +1.5 5,843,087 - Psyr_4930 0.18 +3.3 5,843,098 + Psyr_4930 0.18 -1.8 5,843,098 + Psyr_4930 0.18 -3.3 5,843,098 + Psyr_4930 0.18 -2.8 5,843,098 + Psyr_4930 0.18 +1.3 5,843,098 + Psyr_4930 0.18 +0.6 5,843,098 + Psyr_4930 0.18 -1.2 5,843,099 - Psyr_4930 0.19 +3.3 5,843,099 - Psyr_4930 0.19 +2.3 5,843,099 - Psyr_4930 0.19 -1.7 5,843,102 + Psyr_4930 0.19 -0.9 5,843,102 + Psyr_4930 0.19 -0.1 5,843,102 + Psyr_4930 0.19 +3.1 5,843,103 - Psyr_4930 0.19 -0.4 5,843,103 - Psyr_4930 0.19 +4.4 5,843,141 - Psyr_4930 0.22 -1.0 5,843,188 + Psyr_4930 0.25 -1.7 5,843,188 + Psyr_4930 0.25 -2.3 5,843,203 + Psyr_4930 0.27 +1.8 5,843,203 + Psyr_4930 0.27 -0.2 5,843,203 + Psyr_4930 0.27 -2.1 5,843,204 - Psyr_4930 0.27 -0.5 5,843,204 - Psyr_4930 0.27 -0.7 5,843,204 - Psyr_4930 0.27 -1.7 5,843,204 - Psyr_4930 0.27 +0.8 5,843,204 - Psyr_4930 0.27 -0.0 5,843,204 - Psyr_4930 0.27 +0.4 5,843,261 - Psyr_4930 0.31 +4.5 5,843,446 + Psyr_4930 0.46 +0.6 5,843,446 + Psyr_4930 0.46 -1.1 5,843,446 + Psyr_4930 0.46 -1.6 5,843,446 + Psyr_4930 0.46 -0.5 5,843,446 + Psyr_4930 0.46 -1.6 5,843,446 + Psyr_4930 0.46 +2.8 5,843,446 + Psyr_4930 0.46 -0.7 5,843,446 + Psyr_4930 0.46 -2.1 5,843,446 + Psyr_4930 0.46 +2.6 5,843,447 - Psyr_4930 0.46 -1.4 5,843,447 - Psyr_4930 0.46 +0.4 5,843,447 - Psyr_4930 0.46 -0.6 5,843,447 - Psyr_4930 0.46 -2.2 5,843,447 - Psyr_4930 0.46 -0.7 5,843,447 - Psyr_4930 0.46 +0.3 5,843,447 - Psyr_4930 0.46 -0.5 5,843,447 - Psyr_4930 0.46 -1.5 5,843,447 - Psyr_4930 0.46 -0.1 5,843,447 - Psyr_4930 0.46 -1.6 5,843,447 - Psyr_4930 0.46 -0.9 5,843,447 - Psyr_4930 0.46 -1.4 5,843,447 - Psyr_4930 0.46 +0.3 5,843,447 - Psyr_4930 0.46 -3.6 5,843,447 - Psyr_4930 0.46 -0.9 5,843,462 - Psyr_4930 0.47 -3.1 5,843,497 + Psyr_4930 0.50 +0.1 5,843,497 + Psyr_4930 0.50 -1.0 5,843,498 - Psyr_4930 0.50 +0.0 5,843,498 - Psyr_4930 0.50 -0.6 5,843,498 - Psyr_4930 0.50 -0.7 5,843,498 - Psyr_4930 0.50 +3.3 5,843,530 + Psyr_4930 0.52 -0.6
Or see this region's nucleotide sequence