Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4817 and Psyr_4818 are separated by 163 nucleotides Psyr_4818 and Psyr_4819 are separated by 32 nucleotides Psyr_4819 and Psyr_4820 overlap by 4 nucleotides Psyr_4820 and Psyr_4821 are separated by 282 nucleotides
Psyr_4817: Psyr_4817 - L-glutamine synthetase, at 5,714,118 to 5,715,524
_4817
Psyr_4818: Psyr_4818 - chorismate mutase, at 5,715,688 to 5,716,245
_4818
Psyr_4819: Psyr_4819 - conserved hypothetical protein, at 5,716,278 to 5,716,859
_4819
Psyr_4820: Psyr_4820 - conserved hypothetical protein, at 5,716,856 to 5,717,488
_4820
Psyr_4821: Psyr_4821 - PAS/PAC sensor signal transduction histidine kinase, at 5,717,771 to 5,718,856
_4821
Position (kb) 5716
5717 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 5715.551 kb on - strand at 5715.650 kb on - strand at 5715.697 kb on - strand at 5715.697 kb on - strand at 5715.735 kb on + strand at 5715.736 kb on - strand at 5715.736 kb on - strand at 5715.976 kb on - strand, within Psyr_4818 at 5716.284 kb on + strand at 5716.284 kb on + strand at 5716.284 kb on + strand at 5716.284 kb on + strand at 5716.284 kb on + strand at 5716.285 kb on - strand at 5716.285 kb on - strand at 5716.285 kb on - strand at 5716.285 kb on - strand at 5716.285 kb on - strand at 5716.285 kb on - strand at 5716.285 kb on - strand at 5716.285 kb on - strand at 5716.285 kb on - strand at 5716.285 kb on - strand at 5716.285 kb on - strand at 5716.383 kb on + strand, within Psyr_4819 at 5716.383 kb on + strand, within Psyr_4819 at 5716.383 kb on + strand, within Psyr_4819 at 5716.383 kb on + strand, within Psyr_4819 at 5716.383 kb on + strand, within Psyr_4819 at 5716.383 kb on + strand, within Psyr_4819 at 5716.383 kb on + strand, within Psyr_4819 at 5716.383 kb on + strand, within Psyr_4819 at 5716.383 kb on + strand, within Psyr_4819 at 5716.383 kb on + strand, within Psyr_4819 at 5716.384 kb on - strand, within Psyr_4819 at 5716.384 kb on - strand, within Psyr_4819 at 5716.384 kb on - strand, within Psyr_4819 at 5716.384 kb on - strand, within Psyr_4819 at 5716.384 kb on - strand, within Psyr_4819 at 5716.384 kb on - strand, within Psyr_4819 at 5716.384 kb on - strand, within Psyr_4819 at 5716.384 kb on - strand, within Psyr_4819 at 5716.384 kb on - strand, within Psyr_4819 at 5716.384 kb on - strand, within Psyr_4819 at 5716.384 kb on - strand, within Psyr_4819 at 5716.384 kb on - strand, within Psyr_4819 at 5716.384 kb on - strand, within Psyr_4819 at 5716.384 kb on - strand, within Psyr_4819 at 5716.384 kb on - strand, within Psyr_4819 at 5716.384 kb on - strand, within Psyr_4819 at 5716.385 kb on + strand, within Psyr_4819 at 5716.385 kb on + strand, within Psyr_4819 at 5716.385 kb on + strand, within Psyr_4819 at 5716.385 kb on + strand, within Psyr_4819 at 5716.385 kb on + strand, within Psyr_4819 at 5716.386 kb on - strand, within Psyr_4819 at 5716.386 kb on - strand, within Psyr_4819 at 5716.386 kb on - strand, within Psyr_4819 at 5716.386 kb on - strand, within Psyr_4819 at 5716.386 kb on - strand, within Psyr_4819 at 5716.386 kb on - strand, within Psyr_4819 at 5716.389 kb on + strand, within Psyr_4819 at 5716.389 kb on + strand, within Psyr_4819 at 5716.389 kb on + strand, within Psyr_4819 at 5716.390 kb on - strand, within Psyr_4819 at 5716.390 kb on - strand, within Psyr_4819 at 5716.566 kb on + strand, within Psyr_4819 at 5716.705 kb on - strand, within Psyr_4819 at 5716.831 kb on + strand at 5716.831 kb on + strand at 5716.840 kb on + strand at 5716.841 kb on - strand at 5716.931 kb on + strand, within Psyr_4820 at 5716.932 kb on - strand, within Psyr_4820 at 5716.932 kb on - strand, within Psyr_4820 at 5716.932 kb on - strand, within Psyr_4820 at 5716.932 kb on - strand, within Psyr_4820 at 5716.932 kb on - strand, within Psyr_4820 at 5716.932 kb on - strand, within Psyr_4820 at 5716.937 kb on + strand, within Psyr_4820 at 5716.937 kb on + strand, within Psyr_4820 at 5716.937 kb on + strand, within Psyr_4820 at 5716.937 kb on + strand, within Psyr_4820 at 5716.937 kb on + strand, within Psyr_4820 at 5716.937 kb on + strand, within Psyr_4820 at 5716.937 kb on + strand, within Psyr_4820 at 5716.937 kb on + strand, within Psyr_4820 at 5716.937 kb on + strand, within Psyr_4820 at 5716.937 kb on + strand, within Psyr_4820 at 5716.937 kb on + strand, within Psyr_4820 at 5716.937 kb on + strand, within Psyr_4820 at 5716.937 kb on + strand, within Psyr_4820 at 5716.937 kb on + strand, within Psyr_4820 at 5716.937 kb on + strand, within Psyr_4820 at 5716.937 kb on + strand, within Psyr_4820 at 5716.938 kb on - strand, within Psyr_4820 at 5716.938 kb on - strand, within Psyr_4820 at 5716.938 kb on - strand, within Psyr_4820 at 5716.938 kb on - strand, within Psyr_4820 at 5716.938 kb on - strand, within Psyr_4820 at 5716.938 kb on - strand, within Psyr_4820 at 5716.938 kb on - strand, within Psyr_4820 at 5716.938 kb on - strand, within Psyr_4820 at 5716.938 kb on - strand, within Psyr_4820 at 5716.938 kb on - strand, within Psyr_4820 at 5716.938 kb on - strand, within Psyr_4820 at 5716.938 kb on - strand, within Psyr_4820 at 5716.938 kb on - strand, within Psyr_4820 at 5716.938 kb on - strand, within Psyr_4820 at 5716.938 kb on - strand, within Psyr_4820 at 5716.938 kb on - strand, within Psyr_4820 at 5716.938 kb on - strand, within Psyr_4820 at 5716.938 kb on - strand, within Psyr_4820 at 5716.938 kb on - strand, within Psyr_4820 at 5716.938 kb on - strand, within Psyr_4820 at 5717.076 kb on + strand, within Psyr_4820 at 5717.076 kb on + strand, within Psyr_4820 at 5717.077 kb on - strand, within Psyr_4820 at 5717.132 kb on - strand, within Psyr_4820 at 5717.417 kb on + strand, within Psyr_4820 at 5717.418 kb on - strand, within Psyr_4820 at 5717.418 kb on - strand, within Psyr_4820 at 5717.418 kb on - strand, within Psyr_4820 at 5717.544 kb on + strand at 5717.544 kb on + strand at 5717.587 kb on - strand at 5717.587 kb on - strand at 5717.626 kb on + strand at 5717.627 kb on - strand at 5717.686 kb on - strand at 5717.686 kb on - strand at 5717.858 kb on + strand at 5717.858 kb on + strand at 5717.858 kb on + strand at 5717.858 kb on + strand at 5717.858 kb on + strand at 5717.858 kb on + strand at 5717.859 kb on - strand at 5717.859 kb on - strand at 5717.859 kb on - strand at 5717.859 kb on - strand at 5717.859 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 5,715,551 - +3.0 5,715,650 - -0.6 5,715,697 - +1.2 5,715,697 - -1.4 5,715,735 + -3.7 5,715,736 - +0.3 5,715,736 - +2.2 5,715,976 - Psyr_4818 0.52 +3.0 5,716,284 + -0.8 5,716,284 + +3.0 5,716,284 + -1.8 5,716,284 + +1.8 5,716,284 + +0.2 5,716,285 - +0.6 5,716,285 - -0.9 5,716,285 - -0.9 5,716,285 - -0.7 5,716,285 - +2.8 5,716,285 - +3.7 5,716,285 - +3.7 5,716,285 - +4.5 5,716,285 - +0.1 5,716,285 - +3.5 5,716,285 - -1.3 5,716,383 + Psyr_4819 0.18 -2.9 5,716,383 + Psyr_4819 0.18 -2.6 5,716,383 + Psyr_4819 0.18 -0.9 5,716,383 + Psyr_4819 0.18 -2.4 5,716,383 + Psyr_4819 0.18 -2.1 5,716,383 + Psyr_4819 0.18 -0.5 5,716,383 + Psyr_4819 0.18 -1.3 5,716,383 + Psyr_4819 0.18 -1.8 5,716,383 + Psyr_4819 0.18 -0.2 5,716,383 + Psyr_4819 0.18 +2.5 5,716,384 - Psyr_4819 0.18 -0.6 5,716,384 - Psyr_4819 0.18 +2.5 5,716,384 - Psyr_4819 0.18 -1.8 5,716,384 - Psyr_4819 0.18 -2.3 5,716,384 - Psyr_4819 0.18 +1.2 5,716,384 - Psyr_4819 0.18 -1.2 5,716,384 - Psyr_4819 0.18 -2.1 5,716,384 - Psyr_4819 0.18 -2.1 5,716,384 - Psyr_4819 0.18 +0.2 5,716,384 - Psyr_4819 0.18 -1.7 5,716,384 - Psyr_4819 0.18 +3.8 5,716,384 - Psyr_4819 0.18 -1.8 5,716,384 - Psyr_4819 0.18 -2.0 5,716,384 - Psyr_4819 0.18 -0.6 5,716,384 - Psyr_4819 0.18 -2.7 5,716,384 - Psyr_4819 0.18 -4.1 5,716,385 + Psyr_4819 0.18 +1.8 5,716,385 + Psyr_4819 0.18 -1.7 5,716,385 + Psyr_4819 0.18 -0.6 5,716,385 + Psyr_4819 0.18 -1.8 5,716,385 + Psyr_4819 0.18 -1.2 5,716,386 - Psyr_4819 0.19 -2.6 5,716,386 - Psyr_4819 0.19 -2.1 5,716,386 - Psyr_4819 0.19 -1.3 5,716,386 - Psyr_4819 0.19 +0.9 5,716,386 - Psyr_4819 0.19 -3.5 5,716,386 - Psyr_4819 0.19 -2.5 5,716,389 + Psyr_4819 0.19 +0.2 5,716,389 + Psyr_4819 0.19 -1.4 5,716,389 + Psyr_4819 0.19 +1.2 5,716,390 - Psyr_4819 0.19 -1.2 5,716,390 - Psyr_4819 0.19 +2.5 5,716,566 + Psyr_4819 0.49 -2.2 5,716,705 - Psyr_4819 0.73 +0.0 5,716,831 + -0.2 5,716,831 + -1.1 5,716,840 + +2.8 5,716,841 - +2.8 5,716,931 + Psyr_4820 0.12 +1.3 5,716,932 - Psyr_4820 0.12 +3.2 5,716,932 - Psyr_4820 0.12 -1.1 5,716,932 - Psyr_4820 0.12 +1.3 5,716,932 - Psyr_4820 0.12 -0.4 5,716,932 - Psyr_4820 0.12 +1.8 5,716,932 - Psyr_4820 0.12 -2.4 5,716,937 + Psyr_4820 0.13 -0.4 5,716,937 + Psyr_4820 0.13 -1.2 5,716,937 + Psyr_4820 0.13 -0.7 5,716,937 + Psyr_4820 0.13 +3.2 5,716,937 + Psyr_4820 0.13 -0.0 5,716,937 + Psyr_4820 0.13 -0.6 5,716,937 + Psyr_4820 0.13 -0.7 5,716,937 + Psyr_4820 0.13 -0.4 5,716,937 + Psyr_4820 0.13 -0.4 5,716,937 + Psyr_4820 0.13 -1.2 5,716,937 + Psyr_4820 0.13 -0.7 5,716,937 + Psyr_4820 0.13 -0.8 5,716,937 + Psyr_4820 0.13 -1.2 5,716,937 + Psyr_4820 0.13 -0.6 5,716,937 + Psyr_4820 0.13 -1.2 5,716,937 + Psyr_4820 0.13 -0.9 5,716,938 - Psyr_4820 0.13 +3.8 5,716,938 - Psyr_4820 0.13 -0.2 5,716,938 - Psyr_4820 0.13 +0.2 5,716,938 - Psyr_4820 0.13 +0.2 5,716,938 - Psyr_4820 0.13 +4.5 5,716,938 - Psyr_4820 0.13 +1.1 5,716,938 - Psyr_4820 0.13 +0.2 5,716,938 - Psyr_4820 0.13 +1.2 5,716,938 - Psyr_4820 0.13 +3.4 5,716,938 - Psyr_4820 0.13 +1.2 5,716,938 - Psyr_4820 0.13 +1.7 5,716,938 - Psyr_4820 0.13 +3.4 5,716,938 - Psyr_4820 0.13 -0.7 5,716,938 - Psyr_4820 0.13 +0.1 5,716,938 - Psyr_4820 0.13 -3.5 5,716,938 - Psyr_4820 0.13 +2.8 5,716,938 - Psyr_4820 0.13 -1.0 5,716,938 - Psyr_4820 0.13 -0.2 5,716,938 - Psyr_4820 0.13 -0.3 5,716,938 - Psyr_4820 0.13 -0.9 5,717,076 + Psyr_4820 0.35 -1.1 5,717,076 + Psyr_4820 0.35 +1.6 5,717,077 - Psyr_4820 0.35 -0.8 5,717,132 - Psyr_4820 0.44 +2.2 5,717,417 + Psyr_4820 0.89 +1.2 5,717,418 - Psyr_4820 0.89 -2.0 5,717,418 - Psyr_4820 0.89 +1.2 5,717,418 - Psyr_4820 0.89 -2.2 5,717,544 + +1.7 5,717,544 + +4.7 5,717,587 - +1.0 5,717,587 - -1.5 5,717,626 + +4.1 5,717,627 - +4.4 5,717,686 - +0.2 5,717,686 - -0.9 5,717,858 + -1.1 5,717,858 + +0.3 5,717,858 + -1.7 5,717,858 + +0.5 5,717,858 + -0.1 5,717,858 + -1.6 5,717,859 - -0.9 5,717,859 - -0.6 5,717,859 - -1.6 5,717,859 - +3.8 5,717,859 - +3.4
Or see this region's nucleotide sequence