Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4749 and Psyr_4750 overlap by 4 nucleotides Psyr_4750 and Psyr_4751 overlap by 4 nucleotides
Psyr_4749: Psyr_4749 - cell division protein FtsX, at 5,624,736 to 5,625,770
_4749
Psyr_4750: Psyr_4750 - cell division ATP-binding protein FtsE, at 5,625,767 to 5,626,438
_4750
Psyr_4751: Psyr_4751 - signal recognition particle-docking protein FtsY, at 5,626,435 to 5,627,952
_4751
Position (kb)
5625
5626
5627 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 5624.792 kb on - strand at 5624.792 kb on - strand at 5624.792 kb on - strand at 5624.869 kb on + strand, within Psyr_4749 at 5624.917 kb on + strand, within Psyr_4749 at 5624.918 kb on - strand, within Psyr_4749 at 5624.972 kb on + strand, within Psyr_4749 at 5624.973 kb on - strand, within Psyr_4749 at 5624.974 kb on + strand, within Psyr_4749 at 5624.974 kb on + strand, within Psyr_4749 at 5624.974 kb on + strand, within Psyr_4749 at 5624.975 kb on - strand, within Psyr_4749 at 5624.975 kb on - strand, within Psyr_4749 at 5624.975 kb on - strand, within Psyr_4749 at 5624.975 kb on - strand, within Psyr_4749 at 5624.975 kb on - strand, within Psyr_4749 at 5624.975 kb on - strand, within Psyr_4749 at 5624.975 kb on - strand, within Psyr_4749 at 5624.975 kb on - strand, within Psyr_4749 at 5624.975 kb on - strand, within Psyr_4749 at 5624.995 kb on + strand, within Psyr_4749 at 5624.995 kb on + strand, within Psyr_4749 at 5624.995 kb on + strand, within Psyr_4749 at 5624.995 kb on + strand, within Psyr_4749 at 5624.996 kb on - strand, within Psyr_4749 at 5624.996 kb on - strand, within Psyr_4749 at 5624.996 kb on - strand, within Psyr_4749 at 5624.996 kb on - strand, within Psyr_4749 at 5624.996 kb on - strand, within Psyr_4749 at 5624.996 kb on - strand, within Psyr_4749 at 5624.996 kb on - strand, within Psyr_4749 at 5624.996 kb on - strand, within Psyr_4749 at 5624.996 kb on - strand, within Psyr_4749 at 5624.996 kb on - strand, within Psyr_4749 at 5624.996 kb on - strand, within Psyr_4749 at 5624.996 kb on - strand, within Psyr_4749 at 5624.996 kb on - strand, within Psyr_4749 at 5624.996 kb on - strand, within Psyr_4749 at 5624.996 kb on - strand, within Psyr_4749 at 5624.996 kb on - strand, within Psyr_4749 at 5624.996 kb on - strand, within Psyr_4749 at 5624.996 kb on - strand, within Psyr_4749 at 5625.057 kb on + strand, within Psyr_4749 at 5625.058 kb on - strand, within Psyr_4749 at 5625.058 kb on - strand, within Psyr_4749 at 5625.078 kb on + strand, within Psyr_4749 at 5625.078 kb on + strand, within Psyr_4749 at 5625.078 kb on + strand, within Psyr_4749 at 5625.078 kb on + strand, within Psyr_4749 at 5625.079 kb on - strand, within Psyr_4749 at 5625.079 kb on - strand, within Psyr_4749 at 5625.079 kb on - strand, within Psyr_4749 at 5625.193 kb on - strand, within Psyr_4749 at 5625.193 kb on - strand, within Psyr_4749 at 5625.193 kb on - strand, within Psyr_4749 at 5625.264 kb on + strand, within Psyr_4749 at 5625.264 kb on + strand, within Psyr_4749 at 5625.364 kb on + strand, within Psyr_4749 at 5625.364 kb on + strand, within Psyr_4749 at 5625.364 kb on + strand, within Psyr_4749 at 5625.364 kb on + strand, within Psyr_4749 at 5625.364 kb on + strand, within Psyr_4749 at 5625.364 kb on + strand, within Psyr_4749 at 5625.365 kb on - strand, within Psyr_4749 at 5625.365 kb on - strand, within Psyr_4749 at 5625.365 kb on - strand, within Psyr_4749 at 5625.365 kb on - strand, within Psyr_4749 at 5625.449 kb on - strand, within Psyr_4749 at 5625.449 kb on - strand, within Psyr_4749 at 5625.561 kb on - strand, within Psyr_4749 at 5625.770 kb on + strand at 5625.770 kb on + strand at 5625.771 kb on - strand at 5625.771 kb on - strand at 5625.771 kb on - strand at 5625.771 kb on - strand at 5625.892 kb on + strand, within Psyr_4750 at 5626.038 kb on + strand, within Psyr_4750 at 5626.364 kb on + strand, within Psyr_4750
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 5,624,792 - -1.5 5,624,792 - +3.0 5,624,792 - -1.0 5,624,869 + Psyr_4749 0.13 -1.1 5,624,917 + Psyr_4749 0.17 +1.1 5,624,918 - Psyr_4749 0.18 -3.0 5,624,972 + Psyr_4749 0.23 +2.2 5,624,973 - Psyr_4749 0.23 +0.2 5,624,974 + Psyr_4749 0.23 -1.3 5,624,974 + Psyr_4749 0.23 -2.3 5,624,974 + Psyr_4749 0.23 -1.6 5,624,975 - Psyr_4749 0.23 -1.6 5,624,975 - Psyr_4749 0.23 -1.3 5,624,975 - Psyr_4749 0.23 -1.6 5,624,975 - Psyr_4749 0.23 +3.0 5,624,975 - Psyr_4749 0.23 -2.3 5,624,975 - Psyr_4749 0.23 +3.9 5,624,975 - Psyr_4749 0.23 +1.8 5,624,975 - Psyr_4749 0.23 -0.4 5,624,975 - Psyr_4749 0.23 +1.2 5,624,995 + Psyr_4749 0.25 +0.1 5,624,995 + Psyr_4749 0.25 -1.1 5,624,995 + Psyr_4749 0.25 -1.2 5,624,995 + Psyr_4749 0.25 -0.9 5,624,996 - Psyr_4749 0.25 +3.6 5,624,996 - Psyr_4749 0.25 -2.2 5,624,996 - Psyr_4749 0.25 -0.8 5,624,996 - Psyr_4749 0.25 -0.7 5,624,996 - Psyr_4749 0.25 -0.6 5,624,996 - Psyr_4749 0.25 +0.2 5,624,996 - Psyr_4749 0.25 -0.0 5,624,996 - Psyr_4749 0.25 -0.8 5,624,996 - Psyr_4749 0.25 +1.2 5,624,996 - Psyr_4749 0.25 +2.2 5,624,996 - Psyr_4749 0.25 -1.6 5,624,996 - Psyr_4749 0.25 -1.0 5,624,996 - Psyr_4749 0.25 +1.3 5,624,996 - Psyr_4749 0.25 -1.6 5,624,996 - Psyr_4749 0.25 -2.4 5,624,996 - Psyr_4749 0.25 +3.7 5,624,996 - Psyr_4749 0.25 +0.7 5,624,996 - Psyr_4749 0.25 +0.2 5,625,057 + Psyr_4749 0.31 +0.6 5,625,058 - Psyr_4749 0.31 +5.0 5,625,058 - Psyr_4749 0.31 -0.9 5,625,078 + Psyr_4749 0.33 -1.9 5,625,078 + Psyr_4749 0.33 -0.8 5,625,078 + Psyr_4749 0.33 -3.4 5,625,078 + Psyr_4749 0.33 +3.0 5,625,079 - Psyr_4749 0.33 +2.2 5,625,079 - Psyr_4749 0.33 +1.0 5,625,079 - Psyr_4749 0.33 -3.2 5,625,193 - Psyr_4749 0.44 -0.2 5,625,193 - Psyr_4749 0.44 +3.4 5,625,193 - Psyr_4749 0.44 -0.1 5,625,264 + Psyr_4749 0.51 +4.8 5,625,264 + Psyr_4749 0.51 -1.0 5,625,364 + Psyr_4749 0.61 -0.4 5,625,364 + Psyr_4749 0.61 -1.8 5,625,364 + Psyr_4749 0.61 +2.2 5,625,364 + Psyr_4749 0.61 -0.7 5,625,364 + Psyr_4749 0.61 -1.4 5,625,364 + Psyr_4749 0.61 +3.6 5,625,365 - Psyr_4749 0.61 +2.8 5,625,365 - Psyr_4749 0.61 -1.5 5,625,365 - Psyr_4749 0.61 -1.9 5,625,365 - Psyr_4749 0.61 -0.6 5,625,449 - Psyr_4749 0.69 +2.2 5,625,449 - Psyr_4749 0.69 +3.7 5,625,561 - Psyr_4749 0.80 +1.8 5,625,770 + -0.6 5,625,770 + -0.9 5,625,771 - -0.2 5,625,771 - -1.5 5,625,771 - +3.4 5,625,771 - -1.0 5,625,892 + Psyr_4750 0.19 -2.3 5,626,038 + Psyr_4750 0.40 -0.6 5,626,364 + Psyr_4750 0.89 -1.3
Or see this region's nucleotide sequence