Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4712 and Psyr_4713 are separated by 17 nucleotides Psyr_4713 and Psyr_4714 are separated by 15 nucleotides Psyr_4714 and Psyr_4715 overlap by 4 nucleotides
Psyr_4712: Psyr_4712 - serine hydroxymethyltransferase, at 5,583,431 to 5,584,684
_4712
Psyr_4713: Psyr_4713 - Sarcosine oxidase, beta subunit, heterotetrameric, at 5,584,702 to 5,585,952
_4713
Psyr_4714: Psyr_4714 - Sarcosine oxidase, delta subunit, heterotetrameric, at 5,585,968 to 5,586,264
_4714
Psyr_4715: Psyr_4715 - Sarcosine oxidase, alpha subunit, heterotetrameric, at 5,586,261 to 5,589,281
_4715
Position (kb) 5584
5585
5586 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 5583.747 kb on - strand, within Psyr_4712 at 5583.848 kb on + strand, within Psyr_4712 at 5583.848 kb on + strand, within Psyr_4712 at 5583.848 kb on + strand, within Psyr_4712 at 5583.849 kb on - strand, within Psyr_4712 at 5583.849 kb on - strand, within Psyr_4712 at 5583.849 kb on - strand, within Psyr_4712 at 5583.849 kb on - strand, within Psyr_4712 at 5583.851 kb on - strand, within Psyr_4712 at 5583.962 kb on + strand, within Psyr_4712 at 5584.019 kb on + strand, within Psyr_4712 at 5584.020 kb on - strand, within Psyr_4712 at 5584.020 kb on - strand, within Psyr_4712 at 5584.089 kb on - strand, within Psyr_4712 at 5584.098 kb on + strand, within Psyr_4712 at 5584.098 kb on + strand, within Psyr_4712 at 5584.098 kb on + strand, within Psyr_4712 at 5584.098 kb on + strand, within Psyr_4712 at 5584.098 kb on + strand, within Psyr_4712 at 5584.098 kb on + strand, within Psyr_4712 at 5584.098 kb on + strand, within Psyr_4712 at 5584.098 kb on + strand, within Psyr_4712 at 5584.099 kb on - strand, within Psyr_4712 at 5584.099 kb on - strand, within Psyr_4712 at 5584.099 kb on - strand, within Psyr_4712 at 5584.099 kb on - strand, within Psyr_4712 at 5584.099 kb on - strand, within Psyr_4712 at 5584.289 kb on + strand, within Psyr_4712 at 5584.290 kb on - strand, within Psyr_4712 at 5584.693 kb on + strand at 5584.711 kb on + strand at 5584.799 kb on - strand at 5584.799 kb on - strand at 5584.846 kb on + strand, within Psyr_4713 at 5584.846 kb on + strand, within Psyr_4713 at 5584.849 kb on + strand, within Psyr_4713 at 5584.849 kb on + strand, within Psyr_4713 at 5584.850 kb on - strand, within Psyr_4713 at 5584.850 kb on - strand, within Psyr_4713 at 5584.850 kb on - strand, within Psyr_4713 at 5584.951 kb on + strand, within Psyr_4713 at 5584.951 kb on + strand, within Psyr_4713 at 5584.952 kb on - strand, within Psyr_4713 at 5584.952 kb on - strand, within Psyr_4713 at 5584.952 kb on - strand, within Psyr_4713 at 5584.952 kb on - strand, within Psyr_4713 at 5584.978 kb on + strand, within Psyr_4713 at 5584.978 kb on + strand, within Psyr_4713 at 5584.979 kb on - strand, within Psyr_4713 at 5585.026 kb on + strand, within Psyr_4713 at 5585.026 kb on + strand, within Psyr_4713 at 5585.026 kb on + strand, within Psyr_4713 at 5585.026 kb on + strand, within Psyr_4713 at 5585.027 kb on - strand, within Psyr_4713 at 5585.027 kb on - strand, within Psyr_4713 at 5585.027 kb on - strand, within Psyr_4713 at 5585.027 kb on - strand, within Psyr_4713 at 5585.056 kb on + strand, within Psyr_4713 at 5585.056 kb on + strand, within Psyr_4713 at 5585.057 kb on - strand, within Psyr_4713 at 5585.057 kb on - strand, within Psyr_4713 at 5585.057 kb on - strand, within Psyr_4713 at 5585.548 kb on + strand, within Psyr_4713 at 5585.548 kb on + strand, within Psyr_4713 at 5585.549 kb on - strand, within Psyr_4713 at 5585.549 kb on - strand, within Psyr_4713 at 5585.976 kb on + strand at 5585.976 kb on + strand at 5585.976 kb on + strand at 5585.976 kb on + strand at 5585.976 kb on + strand at 5585.976 kb on + strand at 5585.976 kb on + strand at 5585.977 kb on - strand at 5585.977 kb on - strand at 5585.977 kb on - strand at 5586.094 kb on + strand, within Psyr_4714 at 5586.094 kb on + strand, within Psyr_4714 at 5586.094 kb on + strand, within Psyr_4714 at 5586.094 kb on + strand, within Psyr_4714 at 5586.094 kb on + strand, within Psyr_4714 at 5586.095 kb on - strand, within Psyr_4714 at 5586.095 kb on - strand, within Psyr_4714 at 5586.095 kb on - strand, within Psyr_4714 at 5586.095 kb on - strand, within Psyr_4714 at 5586.095 kb on - strand, within Psyr_4714 at 5586.095 kb on - strand, within Psyr_4714 at 5586.095 kb on - strand, within Psyr_4714 at 5586.095 kb on - strand, within Psyr_4714 at 5586.095 kb on - strand, within Psyr_4714 at 5586.095 kb on - strand, within Psyr_4714 at 5586.143 kb on + strand, within Psyr_4714 at 5586.143 kb on + strand, within Psyr_4714 at 5586.167 kb on - strand, within Psyr_4714 at 5586.167 kb on - strand, within Psyr_4714 at 5586.196 kb on + strand, within Psyr_4714 at 5586.196 kb on + strand, within Psyr_4714 at 5586.197 kb on - strand, within Psyr_4714 at 5586.260 kb on + strand at 5586.260 kb on + strand at 5586.260 kb on + strand at 5586.260 kb on + strand at 5586.260 kb on + strand at 5586.260 kb on + strand at 5586.260 kb on + strand at 5586.261 kb on - strand at 5586.261 kb on - strand at 5586.261 kb on - strand at 5586.323 kb on + strand at 5586.324 kb on - strand at 5586.399 kb on - strand at 5586.420 kb on + strand at 5586.420 kb on + strand at 5586.421 kb on - strand at 5586.421 kb on - strand at 5586.538 kb on - strand at 5586.642 kb on + strand, within Psyr_4715 at 5586.642 kb on + strand, within Psyr_4715 at 5586.643 kb on - strand, within Psyr_4715 at 5586.645 kb on + strand, within Psyr_4715 at 5586.646 kb on - strand, within Psyr_4715 at 5586.660 kb on + strand, within Psyr_4715 at 5586.661 kb on - strand, within Psyr_4715 at 5586.684 kb on + strand, within Psyr_4715 at 5586.685 kb on - strand, within Psyr_4715 at 5586.693 kb on + strand, within Psyr_4715 at 5586.693 kb on + strand, within Psyr_4715 at 5586.694 kb on - strand, within Psyr_4715 at 5586.694 kb on - strand, within Psyr_4715 at 5586.694 kb on - strand, within Psyr_4715 at 5586.694 kb on - strand, within Psyr_4715 at 5586.750 kb on + strand, within Psyr_4715 at 5586.751 kb on - strand, within Psyr_4715
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 5,583,747 - Psyr_4712 0.25 -0.6 5,583,848 + Psyr_4712 0.33 +0.2 5,583,848 + Psyr_4712 0.33 +0.9 5,583,848 + Psyr_4712 0.33 +3.8 5,583,849 - Psyr_4712 0.33 +0.2 5,583,849 - Psyr_4712 0.33 +0.6 5,583,849 - Psyr_4712 0.33 -1.6 5,583,849 - Psyr_4712 0.33 +1.0 5,583,851 - Psyr_4712 0.33 +4.1 5,583,962 + Psyr_4712 0.42 -0.1 5,584,019 + Psyr_4712 0.47 -2.9 5,584,020 - Psyr_4712 0.47 +4.7 5,584,020 - Psyr_4712 0.47 +3.4 5,584,089 - Psyr_4712 0.52 +0.6 5,584,098 + Psyr_4712 0.53 +0.6 5,584,098 + Psyr_4712 0.53 +2.2 5,584,098 + Psyr_4712 0.53 -1.0 5,584,098 + Psyr_4712 0.53 +3.2 5,584,098 + Psyr_4712 0.53 +2.6 5,584,098 + Psyr_4712 0.53 +3.0 5,584,098 + Psyr_4712 0.53 -0.3 5,584,098 + Psyr_4712 0.53 +1.8 5,584,099 - Psyr_4712 0.53 +3.2 5,584,099 - Psyr_4712 0.53 +0.9 5,584,099 - Psyr_4712 0.53 -0.6 5,584,099 - Psyr_4712 0.53 +2.6 5,584,099 - Psyr_4712 0.53 +3.8 5,584,289 + Psyr_4712 0.68 +4.5 5,584,290 - Psyr_4712 0.69 +0.3 5,584,693 + +0.4 5,584,711 + -2.9 5,584,799 - -1.6 5,584,799 - -0.4 5,584,846 + Psyr_4713 0.12 -0.3 5,584,846 + Psyr_4713 0.12 -1.2 5,584,849 + Psyr_4713 0.12 +0.6 5,584,849 + Psyr_4713 0.12 +2.2 5,584,850 - Psyr_4713 0.12 +1.2 5,584,850 - Psyr_4713 0.12 -0.9 5,584,850 - Psyr_4713 0.12 -0.2 5,584,951 + Psyr_4713 0.20 +2.6 5,584,951 + Psyr_4713 0.20 +0.7 5,584,952 - Psyr_4713 0.20 -2.0 5,584,952 - Psyr_4713 0.20 +0.6 5,584,952 - Psyr_4713 0.20 -0.2 5,584,952 - Psyr_4713 0.20 +1.6 5,584,978 + Psyr_4713 0.22 +4.4 5,584,978 + Psyr_4713 0.22 +3.9 5,584,979 - Psyr_4713 0.22 -0.3 5,585,026 + Psyr_4713 0.26 -0.8 5,585,026 + Psyr_4713 0.26 -0.8 5,585,026 + Psyr_4713 0.26 -2.4 5,585,026 + Psyr_4713 0.26 +1.2 5,585,027 - Psyr_4713 0.26 +1.8 5,585,027 - Psyr_4713 0.26 +4.1 5,585,027 - Psyr_4713 0.26 -0.5 5,585,027 - Psyr_4713 0.26 -1.1 5,585,056 + Psyr_4713 0.28 +1.5 5,585,056 + Psyr_4713 0.28 +2.8 5,585,057 - Psyr_4713 0.28 +1.8 5,585,057 - Psyr_4713 0.28 -1.1 5,585,057 - Psyr_4713 0.28 -0.9 5,585,548 + Psyr_4713 0.68 -0.8 5,585,548 + Psyr_4713 0.68 +3.2 5,585,549 - Psyr_4713 0.68 -1.8 5,585,549 - Psyr_4713 0.68 +3.0 5,585,976 + +0.2 5,585,976 + +4.2 5,585,976 + +4.6 5,585,976 + +3.4 5,585,976 + +0.1 5,585,976 + -2.5 5,585,976 + -0.5 5,585,977 - +1.2 5,585,977 - -1.8 5,585,977 - +4.9 5,586,094 + Psyr_4714 0.42 +1.2 5,586,094 + Psyr_4714 0.42 -0.2 5,586,094 + Psyr_4714 0.42 -2.1 5,586,094 + Psyr_4714 0.42 -0.4 5,586,094 + Psyr_4714 0.42 +1.2 5,586,095 - Psyr_4714 0.43 +0.2 5,586,095 - Psyr_4714 0.43 +2.2 5,586,095 - Psyr_4714 0.43 -1.2 5,586,095 - Psyr_4714 0.43 +2.8 5,586,095 - Psyr_4714 0.43 +0.6 5,586,095 - Psyr_4714 0.43 -0.5 5,586,095 - Psyr_4714 0.43 -1.4 5,586,095 - Psyr_4714 0.43 +1.2 5,586,095 - Psyr_4714 0.43 +1.9 5,586,095 - Psyr_4714 0.43 -0.2 5,586,143 + Psyr_4714 0.59 -0.4 5,586,143 + Psyr_4714 0.59 +0.2 5,586,167 - Psyr_4714 0.67 +0.6 5,586,167 - Psyr_4714 0.67 -1.2 5,586,196 + Psyr_4714 0.77 +2.8 5,586,196 + Psyr_4714 0.77 +4.0 5,586,197 - Psyr_4714 0.77 -0.9 5,586,260 + +3.7 5,586,260 + -0.8 5,586,260 + -4.2 5,586,260 + -0.8 5,586,260 + -0.4 5,586,260 + +0.6 5,586,260 + +0.6 5,586,261 - +4.0 5,586,261 - +0.2 5,586,261 - +0.3 5,586,323 + -0.4 5,586,324 - -0.1 5,586,399 - +2.8 5,586,420 + -1.4 5,586,420 + +1.2 5,586,421 - +0.2 5,586,421 - -1.0 5,586,538 - -0.3 5,586,642 + Psyr_4715 0.13 -1.5 5,586,642 + Psyr_4715 0.13 +0.4 5,586,643 - Psyr_4715 0.13 +1.4 5,586,645 + Psyr_4715 0.13 +2.6 5,586,646 - Psyr_4715 0.13 -0.4 5,586,660 + Psyr_4715 0.13 +0.4 5,586,661 - Psyr_4715 0.13 -0.1 5,586,684 + Psyr_4715 0.14 -0.2 5,586,685 - Psyr_4715 0.14 -2.1 5,586,693 + Psyr_4715 0.14 -2.5 5,586,693 + Psyr_4715 0.14 +1.8 5,586,694 - Psyr_4715 0.14 -1.3 5,586,694 - Psyr_4715 0.14 +1.2 5,586,694 - Psyr_4715 0.14 -0.6 5,586,694 - Psyr_4715 0.14 -1.2 5,586,750 + Psyr_4715 0.16 +0.2 5,586,751 - Psyr_4715 0.16 -1.2
Or see this region's nucleotide sequence