Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4711 and Psyr_4712 are separated by 258 nucleotides Psyr_4712 and Psyr_4713 are separated by 17 nucleotides
Psyr_4711: Psyr_4711 - Glycine betaine/L-proline transport ATP-binding subunit, at 5,581,994 to 5,583,172
_4711
Psyr_4712: Psyr_4712 - serine hydroxymethyltransferase, at 5,583,431 to 5,584,684
_4712
Psyr_4713: Psyr_4713 - Sarcosine oxidase, beta subunit, heterotetrameric, at 5,584,702 to 5,585,952
_4713
Position (kb) 5583
5584
5585 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 5582.738 kb on + strand, within Psyr_4711 at 5582.739 kb on - strand, within Psyr_4711 at 5582.780 kb on + strand, within Psyr_4711 at 5582.780 kb on + strand, within Psyr_4711 at 5582.780 kb on + strand, within Psyr_4711 at 5582.780 kb on + strand, within Psyr_4711 at 5582.781 kb on - strand, within Psyr_4711 at 5582.781 kb on - strand, within Psyr_4711 at 5582.781 kb on - strand, within Psyr_4711 at 5582.781 kb on - strand, within Psyr_4711 at 5582.781 kb on - strand, within Psyr_4711 at 5582.781 kb on - strand, within Psyr_4711 at 5582.803 kb on + strand, within Psyr_4711 at 5582.803 kb on + strand, within Psyr_4711 at 5582.803 kb on + strand, within Psyr_4711 at 5582.803 kb on + strand, within Psyr_4711 at 5582.803 kb on + strand, within Psyr_4711 at 5582.803 kb on + strand, within Psyr_4711 at 5582.803 kb on + strand, within Psyr_4711 at 5582.803 kb on + strand, within Psyr_4711 at 5582.804 kb on - strand, within Psyr_4711 at 5582.804 kb on - strand, within Psyr_4711 at 5582.804 kb on - strand, within Psyr_4711 at 5582.804 kb on - strand, within Psyr_4711 at 5582.804 kb on - strand, within Psyr_4711 at 5582.804 kb on - strand, within Psyr_4711 at 5582.804 kb on - strand, within Psyr_4711 at 5582.804 kb on - strand, within Psyr_4711 at 5582.804 kb on - strand, within Psyr_4711 at 5582.804 kb on - strand, within Psyr_4711 at 5582.845 kb on + strand, within Psyr_4711 at 5583.106 kb on + strand at 5583.140 kb on + strand at 5583.277 kb on - strand at 5583.329 kb on + strand at 5583.542 kb on + strand at 5583.542 kb on + strand at 5583.542 kb on + strand at 5583.542 kb on + strand at 5583.542 kb on + strand at 5583.542 kb on + strand at 5583.543 kb on - strand at 5583.543 kb on - strand at 5583.543 kb on - strand at 5583.543 kb on - strand at 5583.543 kb on - strand at 5583.543 kb on - strand at 5583.593 kb on + strand, within Psyr_4712 at 5583.593 kb on + strand, within Psyr_4712 at 5583.593 kb on + strand, within Psyr_4712 at 5583.620 kb on + strand, within Psyr_4712 at 5583.624 kb on - strand, within Psyr_4712 at 5583.698 kb on + strand, within Psyr_4712 at 5583.699 kb on - strand, within Psyr_4712 at 5583.747 kb on - strand, within Psyr_4712 at 5583.848 kb on + strand, within Psyr_4712 at 5583.848 kb on + strand, within Psyr_4712 at 5583.848 kb on + strand, within Psyr_4712 at 5583.849 kb on - strand, within Psyr_4712 at 5583.849 kb on - strand, within Psyr_4712 at 5583.849 kb on - strand, within Psyr_4712 at 5583.849 kb on - strand, within Psyr_4712 at 5583.851 kb on - strand, within Psyr_4712 at 5583.962 kb on + strand, within Psyr_4712 at 5584.019 kb on + strand, within Psyr_4712 at 5584.020 kb on - strand, within Psyr_4712 at 5584.020 kb on - strand, within Psyr_4712 at 5584.089 kb on - strand, within Psyr_4712 at 5584.098 kb on + strand, within Psyr_4712 at 5584.098 kb on + strand, within Psyr_4712 at 5584.098 kb on + strand, within Psyr_4712 at 5584.098 kb on + strand, within Psyr_4712 at 5584.098 kb on + strand, within Psyr_4712 at 5584.098 kb on + strand, within Psyr_4712 at 5584.098 kb on + strand, within Psyr_4712 at 5584.098 kb on + strand, within Psyr_4712 at 5584.099 kb on - strand, within Psyr_4712 at 5584.099 kb on - strand, within Psyr_4712 at 5584.099 kb on - strand, within Psyr_4712 at 5584.099 kb on - strand, within Psyr_4712 at 5584.099 kb on - strand, within Psyr_4712 at 5584.289 kb on + strand, within Psyr_4712 at 5584.290 kb on - strand, within Psyr_4712 at 5584.693 kb on + strand at 5584.711 kb on + strand at 5584.799 kb on - strand at 5584.799 kb on - strand at 5584.846 kb on + strand, within Psyr_4713 at 5584.846 kb on + strand, within Psyr_4713 at 5584.849 kb on + strand, within Psyr_4713 at 5584.849 kb on + strand, within Psyr_4713 at 5584.850 kb on - strand, within Psyr_4713 at 5584.850 kb on - strand, within Psyr_4713 at 5584.850 kb on - strand, within Psyr_4713 at 5584.951 kb on + strand, within Psyr_4713 at 5584.951 kb on + strand, within Psyr_4713 at 5584.952 kb on - strand, within Psyr_4713 at 5584.952 kb on - strand, within Psyr_4713 at 5584.952 kb on - strand, within Psyr_4713 at 5584.952 kb on - strand, within Psyr_4713 at 5584.978 kb on + strand, within Psyr_4713 at 5584.978 kb on + strand, within Psyr_4713 at 5584.979 kb on - strand, within Psyr_4713 at 5585.026 kb on + strand, within Psyr_4713 at 5585.026 kb on + strand, within Psyr_4713 at 5585.026 kb on + strand, within Psyr_4713 at 5585.026 kb on + strand, within Psyr_4713 at 5585.027 kb on - strand, within Psyr_4713 at 5585.027 kb on - strand, within Psyr_4713 at 5585.027 kb on - strand, within Psyr_4713 at 5585.027 kb on - strand, within Psyr_4713 at 5585.056 kb on + strand, within Psyr_4713 at 5585.056 kb on + strand, within Psyr_4713 at 5585.057 kb on - strand, within Psyr_4713 at 5585.057 kb on - strand, within Psyr_4713 at 5585.057 kb on - strand, within Psyr_4713 at 5585.548 kb on + strand, within Psyr_4713 at 5585.548 kb on + strand, within Psyr_4713 at 5585.549 kb on - strand, within Psyr_4713 at 5585.549 kb on - strand, within Psyr_4713
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 5,582,738 + Psyr_4711 0.63 +3.0 5,582,739 - Psyr_4711 0.63 +0.8 5,582,780 + Psyr_4711 0.67 +1.8 5,582,780 + Psyr_4711 0.67 +3.7 5,582,780 + Psyr_4711 0.67 +3.0 5,582,780 + Psyr_4711 0.67 -1.7 5,582,781 - Psyr_4711 0.67 +0.9 5,582,781 - Psyr_4711 0.67 +4.2 5,582,781 - Psyr_4711 0.67 +1.0 5,582,781 - Psyr_4711 0.67 +1.2 5,582,781 - Psyr_4711 0.67 +0.2 5,582,781 - Psyr_4711 0.67 -0.8 5,582,803 + Psyr_4711 0.69 -1.4 5,582,803 + Psyr_4711 0.69 -1.0 5,582,803 + Psyr_4711 0.69 +0.3 5,582,803 + Psyr_4711 0.69 -0.9 5,582,803 + Psyr_4711 0.69 -2.5 5,582,803 + Psyr_4711 0.69 -0.0 5,582,803 + Psyr_4711 0.69 -0.7 5,582,803 + Psyr_4711 0.69 +0.8 5,582,804 - Psyr_4711 0.69 +1.5 5,582,804 - Psyr_4711 0.69 -3.1 5,582,804 - Psyr_4711 0.69 -1.5 5,582,804 - Psyr_4711 0.69 +0.5 5,582,804 - Psyr_4711 0.69 +2.6 5,582,804 - Psyr_4711 0.69 +3.0 5,582,804 - Psyr_4711 0.69 +2.2 5,582,804 - Psyr_4711 0.69 -0.3 5,582,804 - Psyr_4711 0.69 -1.6 5,582,804 - Psyr_4711 0.69 +2.2 5,582,845 + Psyr_4711 0.72 +4.6 5,583,106 + +0.6 5,583,140 + -1.6 5,583,277 - -1.4 5,583,329 + +3.4 5,583,542 + +4.5 5,583,542 + +0.2 5,583,542 + +2.8 5,583,542 + +3.8 5,583,542 + -0.8 5,583,542 + +0.4 5,583,543 - +0.0 5,583,543 - -0.7 5,583,543 - +1.7 5,583,543 - +2.6 5,583,543 - -2.4 5,583,543 - -1.1 5,583,593 + Psyr_4712 0.13 +0.2 5,583,593 + Psyr_4712 0.13 -0.0 5,583,593 + Psyr_4712 0.13 -1.6 5,583,620 + Psyr_4712 0.15 -0.3 5,583,624 - Psyr_4712 0.15 +3.6 5,583,698 + Psyr_4712 0.21 -0.2 5,583,699 - Psyr_4712 0.21 -1.0 5,583,747 - Psyr_4712 0.25 -0.6 5,583,848 + Psyr_4712 0.33 +0.2 5,583,848 + Psyr_4712 0.33 +0.9 5,583,848 + Psyr_4712 0.33 +3.8 5,583,849 - Psyr_4712 0.33 +0.2 5,583,849 - Psyr_4712 0.33 +0.6 5,583,849 - Psyr_4712 0.33 -1.6 5,583,849 - Psyr_4712 0.33 +1.0 5,583,851 - Psyr_4712 0.33 +4.1 5,583,962 + Psyr_4712 0.42 -0.1 5,584,019 + Psyr_4712 0.47 -2.9 5,584,020 - Psyr_4712 0.47 +4.7 5,584,020 - Psyr_4712 0.47 +3.4 5,584,089 - Psyr_4712 0.52 +0.6 5,584,098 + Psyr_4712 0.53 +0.6 5,584,098 + Psyr_4712 0.53 +2.2 5,584,098 + Psyr_4712 0.53 -1.0 5,584,098 + Psyr_4712 0.53 +3.2 5,584,098 + Psyr_4712 0.53 +2.6 5,584,098 + Psyr_4712 0.53 +3.0 5,584,098 + Psyr_4712 0.53 -0.3 5,584,098 + Psyr_4712 0.53 +1.8 5,584,099 - Psyr_4712 0.53 +3.2 5,584,099 - Psyr_4712 0.53 +0.9 5,584,099 - Psyr_4712 0.53 -0.6 5,584,099 - Psyr_4712 0.53 +2.6 5,584,099 - Psyr_4712 0.53 +3.8 5,584,289 + Psyr_4712 0.68 +4.5 5,584,290 - Psyr_4712 0.69 +0.3 5,584,693 + +0.4 5,584,711 + -2.9 5,584,799 - -1.6 5,584,799 - -0.4 5,584,846 + Psyr_4713 0.12 -0.3 5,584,846 + Psyr_4713 0.12 -1.2 5,584,849 + Psyr_4713 0.12 +0.6 5,584,849 + Psyr_4713 0.12 +2.2 5,584,850 - Psyr_4713 0.12 +1.2 5,584,850 - Psyr_4713 0.12 -0.9 5,584,850 - Psyr_4713 0.12 -0.2 5,584,951 + Psyr_4713 0.20 +2.6 5,584,951 + Psyr_4713 0.20 +0.7 5,584,952 - Psyr_4713 0.20 -2.0 5,584,952 - Psyr_4713 0.20 +0.6 5,584,952 - Psyr_4713 0.20 -0.2 5,584,952 - Psyr_4713 0.20 +1.6 5,584,978 + Psyr_4713 0.22 +4.4 5,584,978 + Psyr_4713 0.22 +3.9 5,584,979 - Psyr_4713 0.22 -0.3 5,585,026 + Psyr_4713 0.26 -0.8 5,585,026 + Psyr_4713 0.26 -0.8 5,585,026 + Psyr_4713 0.26 -2.4 5,585,026 + Psyr_4713 0.26 +1.2 5,585,027 - Psyr_4713 0.26 +1.8 5,585,027 - Psyr_4713 0.26 +4.1 5,585,027 - Psyr_4713 0.26 -0.5 5,585,027 - Psyr_4713 0.26 -1.1 5,585,056 + Psyr_4713 0.28 +1.5 5,585,056 + Psyr_4713 0.28 +2.8 5,585,057 - Psyr_4713 0.28 +1.8 5,585,057 - Psyr_4713 0.28 -1.1 5,585,057 - Psyr_4713 0.28 -0.9 5,585,548 + Psyr_4713 0.68 -0.8 5,585,548 + Psyr_4713 0.68 +3.2 5,585,549 - Psyr_4713 0.68 -1.8 5,585,549 - Psyr_4713 0.68 +3.0
Or see this region's nucleotide sequence