Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4709 and Psyr_4710 are separated by 67 nucleotides Psyr_4710 and Psyr_4711 overlap by 4 nucleotides Psyr_4711 and Psyr_4712 are separated by 258 nucleotides
Psyr_4709: Psyr_4709 - Substrate-binding region of ABC-type glycine betaine transport system, at 5,580,137 to 5,581,084
_4709
Psyr_4710: Psyr_4710 - Binding-protein-dependent transport systems inner membrane component, at 5,581,152 to 5,581,997
_4710
Psyr_4711: Psyr_4711 - Glycine betaine/L-proline transport ATP-binding subunit, at 5,581,994 to 5,583,172
_4711
Psyr_4712: Psyr_4712 - serine hydroxymethyltransferase, at 5,583,431 to 5,584,684
_4712
Position (kb) 5581
5582
5583
5584 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 5581.002 kb on + strand at 5581.002 kb on + strand at 5581.002 kb on + strand at 5581.082 kb on + strand at 5581.166 kb on + strand at 5581.166 kb on + strand at 5581.166 kb on + strand at 5581.185 kb on + strand at 5581.186 kb on - strand at 5581.186 kb on - strand at 5581.230 kb on + strand at 5581.230 kb on + strand at 5581.230 kb on + strand at 5581.231 kb on - strand at 5581.411 kb on - strand, within Psyr_4710 at 5581.518 kb on + strand, within Psyr_4710 at 5581.518 kb on + strand, within Psyr_4710 at 5581.518 kb on + strand, within Psyr_4710 at 5581.573 kb on - strand, within Psyr_4710 at 5581.669 kb on - strand, within Psyr_4710 at 5581.669 kb on - strand, within Psyr_4710 at 5581.765 kb on - strand, within Psyr_4710 at 5581.930 kb on + strand at 5581.930 kb on + strand at 5581.931 kb on - strand at 5581.931 kb on - strand at 5581.981 kb on + strand at 5581.982 kb on - strand at 5581.999 kb on + strand at 5581.999 kb on + strand at 5582.000 kb on - strand at 5582.019 kb on + strand at 5582.209 kb on + strand, within Psyr_4711 at 5582.210 kb on - strand, within Psyr_4711 at 5582.738 kb on + strand, within Psyr_4711 at 5582.739 kb on - strand, within Psyr_4711 at 5582.780 kb on + strand, within Psyr_4711 at 5582.780 kb on + strand, within Psyr_4711 at 5582.780 kb on + strand, within Psyr_4711 at 5582.780 kb on + strand, within Psyr_4711 at 5582.781 kb on - strand, within Psyr_4711 at 5582.781 kb on - strand, within Psyr_4711 at 5582.781 kb on - strand, within Psyr_4711 at 5582.781 kb on - strand, within Psyr_4711 at 5582.781 kb on - strand, within Psyr_4711 at 5582.781 kb on - strand, within Psyr_4711 at 5582.803 kb on + strand, within Psyr_4711 at 5582.803 kb on + strand, within Psyr_4711 at 5582.803 kb on + strand, within Psyr_4711 at 5582.803 kb on + strand, within Psyr_4711 at 5582.803 kb on + strand, within Psyr_4711 at 5582.803 kb on + strand, within Psyr_4711 at 5582.803 kb on + strand, within Psyr_4711 at 5582.803 kb on + strand, within Psyr_4711 at 5582.804 kb on - strand, within Psyr_4711 at 5582.804 kb on - strand, within Psyr_4711 at 5582.804 kb on - strand, within Psyr_4711 at 5582.804 kb on - strand, within Psyr_4711 at 5582.804 kb on - strand, within Psyr_4711 at 5582.804 kb on - strand, within Psyr_4711 at 5582.804 kb on - strand, within Psyr_4711 at 5582.804 kb on - strand, within Psyr_4711 at 5582.804 kb on - strand, within Psyr_4711 at 5582.804 kb on - strand, within Psyr_4711 at 5582.845 kb on + strand, within Psyr_4711 at 5583.106 kb on + strand at 5583.140 kb on + strand at 5583.277 kb on - strand at 5583.329 kb on + strand at 5583.542 kb on + strand at 5583.542 kb on + strand at 5583.542 kb on + strand at 5583.542 kb on + strand at 5583.542 kb on + strand at 5583.542 kb on + strand at 5583.543 kb on - strand at 5583.543 kb on - strand at 5583.543 kb on - strand at 5583.543 kb on - strand at 5583.543 kb on - strand at 5583.543 kb on - strand at 5583.593 kb on + strand, within Psyr_4712 at 5583.593 kb on + strand, within Psyr_4712 at 5583.593 kb on + strand, within Psyr_4712 at 5583.620 kb on + strand, within Psyr_4712 at 5583.624 kb on - strand, within Psyr_4712 at 5583.698 kb on + strand, within Psyr_4712 at 5583.699 kb on - strand, within Psyr_4712 at 5583.747 kb on - strand, within Psyr_4712 at 5583.848 kb on + strand, within Psyr_4712 at 5583.848 kb on + strand, within Psyr_4712 at 5583.848 kb on + strand, within Psyr_4712 at 5583.849 kb on - strand, within Psyr_4712 at 5583.849 kb on - strand, within Psyr_4712 at 5583.849 kb on - strand, within Psyr_4712 at 5583.849 kb on - strand, within Psyr_4712 at 5583.851 kb on - strand, within Psyr_4712 at 5583.962 kb on + strand, within Psyr_4712 at 5584.019 kb on + strand, within Psyr_4712 at 5584.020 kb on - strand, within Psyr_4712 at 5584.020 kb on - strand, within Psyr_4712 at 5584.089 kb on - strand, within Psyr_4712 at 5584.098 kb on + strand, within Psyr_4712 at 5584.098 kb on + strand, within Psyr_4712 at 5584.098 kb on + strand, within Psyr_4712 at 5584.098 kb on + strand, within Psyr_4712 at 5584.098 kb on + strand, within Psyr_4712 at 5584.098 kb on + strand, within Psyr_4712 at 5584.098 kb on + strand, within Psyr_4712 at 5584.098 kb on + strand, within Psyr_4712 at 5584.099 kb on - strand, within Psyr_4712 at 5584.099 kb on - strand, within Psyr_4712 at 5584.099 kb on - strand, within Psyr_4712 at 5584.099 kb on - strand, within Psyr_4712 at 5584.099 kb on - strand, within Psyr_4712
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 5,581,002 + +4.3 5,581,002 + +1.5 5,581,002 + -1.5 5,581,082 + -0.1 5,581,166 + -2.5 5,581,166 + +0.2 5,581,166 + -0.5 5,581,185 + +2.2 5,581,186 - +0.7 5,581,186 - +3.6 5,581,230 + +0.2 5,581,230 + -1.2 5,581,230 + -0.9 5,581,231 - -3.0 5,581,411 - Psyr_4710 0.31 +0.5 5,581,518 + Psyr_4710 0.43 -0.6 5,581,518 + Psyr_4710 0.43 +0.2 5,581,518 + Psyr_4710 0.43 -2.4 5,581,573 - Psyr_4710 0.50 -0.6 5,581,669 - Psyr_4710 0.61 +4.1 5,581,669 - Psyr_4710 0.61 -0.6 5,581,765 - Psyr_4710 0.72 -1.6 5,581,930 + -1.1 5,581,930 + -0.3 5,581,931 - +4.0 5,581,931 - -0.1 5,581,981 + +4.7 5,581,982 - +3.4 5,581,999 + +0.7 5,581,999 + -1.4 5,582,000 - +2.2 5,582,019 + +0.2 5,582,209 + Psyr_4711 0.18 +0.8 5,582,210 - Psyr_4711 0.18 +1.2 5,582,738 + Psyr_4711 0.63 +3.0 5,582,739 - Psyr_4711 0.63 +0.8 5,582,780 + Psyr_4711 0.67 +1.8 5,582,780 + Psyr_4711 0.67 +3.7 5,582,780 + Psyr_4711 0.67 +3.0 5,582,780 + Psyr_4711 0.67 -1.7 5,582,781 - Psyr_4711 0.67 +0.9 5,582,781 - Psyr_4711 0.67 +4.2 5,582,781 - Psyr_4711 0.67 +1.0 5,582,781 - Psyr_4711 0.67 +1.2 5,582,781 - Psyr_4711 0.67 +0.2 5,582,781 - Psyr_4711 0.67 -0.8 5,582,803 + Psyr_4711 0.69 -1.4 5,582,803 + Psyr_4711 0.69 -1.0 5,582,803 + Psyr_4711 0.69 +0.3 5,582,803 + Psyr_4711 0.69 -0.9 5,582,803 + Psyr_4711 0.69 -2.5 5,582,803 + Psyr_4711 0.69 -0.0 5,582,803 + Psyr_4711 0.69 -0.7 5,582,803 + Psyr_4711 0.69 +0.8 5,582,804 - Psyr_4711 0.69 +1.5 5,582,804 - Psyr_4711 0.69 -3.1 5,582,804 - Psyr_4711 0.69 -1.5 5,582,804 - Psyr_4711 0.69 +0.5 5,582,804 - Psyr_4711 0.69 +2.6 5,582,804 - Psyr_4711 0.69 +3.0 5,582,804 - Psyr_4711 0.69 +2.2 5,582,804 - Psyr_4711 0.69 -0.3 5,582,804 - Psyr_4711 0.69 -1.6 5,582,804 - Psyr_4711 0.69 +2.2 5,582,845 + Psyr_4711 0.72 +4.6 5,583,106 + +0.6 5,583,140 + -1.6 5,583,277 - -1.4 5,583,329 + +3.4 5,583,542 + +4.5 5,583,542 + +0.2 5,583,542 + +2.8 5,583,542 + +3.8 5,583,542 + -0.8 5,583,542 + +0.4 5,583,543 - +0.0 5,583,543 - -0.7 5,583,543 - +1.7 5,583,543 - +2.6 5,583,543 - -2.4 5,583,543 - -1.1 5,583,593 + Psyr_4712 0.13 +0.2 5,583,593 + Psyr_4712 0.13 -0.0 5,583,593 + Psyr_4712 0.13 -1.6 5,583,620 + Psyr_4712 0.15 -0.3 5,583,624 - Psyr_4712 0.15 +3.6 5,583,698 + Psyr_4712 0.21 -0.2 5,583,699 - Psyr_4712 0.21 -1.0 5,583,747 - Psyr_4712 0.25 -0.6 5,583,848 + Psyr_4712 0.33 +0.2 5,583,848 + Psyr_4712 0.33 +0.9 5,583,848 + Psyr_4712 0.33 +3.8 5,583,849 - Psyr_4712 0.33 +0.2 5,583,849 - Psyr_4712 0.33 +0.6 5,583,849 - Psyr_4712 0.33 -1.6 5,583,849 - Psyr_4712 0.33 +1.0 5,583,851 - Psyr_4712 0.33 +4.1 5,583,962 + Psyr_4712 0.42 -0.1 5,584,019 + Psyr_4712 0.47 -2.9 5,584,020 - Psyr_4712 0.47 +4.7 5,584,020 - Psyr_4712 0.47 +3.4 5,584,089 - Psyr_4712 0.52 +0.6 5,584,098 + Psyr_4712 0.53 +0.6 5,584,098 + Psyr_4712 0.53 +2.2 5,584,098 + Psyr_4712 0.53 -1.0 5,584,098 + Psyr_4712 0.53 +3.2 5,584,098 + Psyr_4712 0.53 +2.6 5,584,098 + Psyr_4712 0.53 +3.0 5,584,098 + Psyr_4712 0.53 -0.3 5,584,098 + Psyr_4712 0.53 +1.8 5,584,099 - Psyr_4712 0.53 +3.2 5,584,099 - Psyr_4712 0.53 +0.9 5,584,099 - Psyr_4712 0.53 -0.6 5,584,099 - Psyr_4712 0.53 +2.6 5,584,099 - Psyr_4712 0.53 +3.8
Or see this region's nucleotide sequence