Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4684 and Psyr_4685 overlap by 8 nucleotides Psyr_4685 and Psyr_4686 overlap by 8 nucleotides Psyr_4686 and Psyr_4687 are separated by 64 nucleotides
Psyr_4684: Psyr_4684 - biotin synthesis protein BioC, at 5,552,061 to 5,552,870
_4684
Psyr_4685: Psyr_4685 - bioH protein, at 5,552,863 to 5,553,594
_4685
Psyr_4686: Psyr_4686 - 8-amino-7-oxononanoate synthase, at 5,553,587 to 5,554,777
_4686
Psyr_4687: Psyr_4687 - biotin synthase, at 5,554,842 to 5,555,900
_4687
Position (kb)
5553
5554
5555 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 5552.630 kb on + strand, within Psyr_4684 at 5552.630 kb on + strand, within Psyr_4684 at 5552.631 kb on - strand, within Psyr_4684 at 5552.782 kb on + strand, within Psyr_4684 at 5553.060 kb on - strand, within Psyr_4685 at 5553.097 kb on + strand, within Psyr_4685 at 5553.098 kb on - strand, within Psyr_4685 at 5553.656 kb on + strand at 5553.659 kb on + strand at 5553.659 kb on + strand at 5553.659 kb on + strand at 5553.659 kb on + strand at 5553.660 kb on - strand at 5553.660 kb on - strand at 5553.660 kb on - strand at 5553.987 kb on + strand, within Psyr_4686 at 5553.987 kb on + strand, within Psyr_4686 at 5553.987 kb on + strand, within Psyr_4686 at 5553.987 kb on + strand, within Psyr_4686 at 5553.987 kb on + strand, within Psyr_4686 at 5553.988 kb on - strand, within Psyr_4686 at 5553.988 kb on - strand, within Psyr_4686 at 5553.988 kb on - strand, within Psyr_4686 at 5553.988 kb on - strand, within Psyr_4686 at 5553.993 kb on + strand, within Psyr_4686 at 5553.993 kb on + strand, within Psyr_4686 at 5553.994 kb on - strand, within Psyr_4686 at 5554.458 kb on + strand, within Psyr_4686 at 5554.458 kb on + strand, within Psyr_4686 at 5554.458 kb on + strand, within Psyr_4686 at 5554.459 kb on - strand, within Psyr_4686 at 5554.459 kb on - strand, within Psyr_4686 at 5554.459 kb on - strand, within Psyr_4686 at 5554.459 kb on - strand, within Psyr_4686 at 5554.638 kb on + strand, within Psyr_4686 at 5554.638 kb on + strand, within Psyr_4686 at 5554.638 kb on + strand, within Psyr_4686 at 5554.639 kb on - strand, within Psyr_4686 at 5554.639 kb on - strand, within Psyr_4686 at 5554.639 kb on - strand, within Psyr_4686 at 5554.639 kb on - strand, within Psyr_4686 at 5554.861 kb on + strand at 5554.861 kb on + strand at 5554.861 kb on + strand at 5554.861 kb on + strand at 5554.862 kb on - strand at 5554.862 kb on - strand at 5554.862 kb on - strand at 5554.862 kb on - strand at 5555.026 kb on + strand, within Psyr_4687 at 5555.129 kb on + strand, within Psyr_4687 at 5555.129 kb on + strand, within Psyr_4687 at 5555.129 kb on + strand at 5555.129 kb on + strand, within Psyr_4687 at 5555.130 kb on - strand, within Psyr_4687 at 5555.130 kb on - strand, within Psyr_4687 at 5555.130 kb on - strand, within Psyr_4687 at 5555.130 kb on - strand, within Psyr_4687 at 5555.347 kb on + strand, within Psyr_4687 at 5555.347 kb on + strand, within Psyr_4687 at 5555.347 kb on + strand, within Psyr_4687 at 5555.347 kb on + strand, within Psyr_4687 at 5555.347 kb on + strand, within Psyr_4687 at 5555.347 kb on + strand, within Psyr_4687 at 5555.348 kb on - strand, within Psyr_4687 at 5555.348 kb on - strand, within Psyr_4687 at 5555.348 kb on - strand, within Psyr_4687 at 5555.348 kb on - strand, within Psyr_4687 at 5555.348 kb on - strand, within Psyr_4687 at 5555.348 kb on - strand, within Psyr_4687 at 5555.374 kb on + strand, within Psyr_4687 at 5555.390 kb on + strand, within Psyr_4687 at 5555.390 kb on + strand, within Psyr_4687 at 5555.391 kb on - strand, within Psyr_4687 at 5555.434 kb on + strand, within Psyr_4687 at 5555.434 kb on + strand at 5555.434 kb on + strand, within Psyr_4687 at 5555.434 kb on + strand, within Psyr_4687 at 5555.434 kb on + strand, within Psyr_4687 at 5555.434 kb on + strand, within Psyr_4687 at 5555.434 kb on + strand, within Psyr_4687 at 5555.435 kb on - strand, within Psyr_4687 at 5555.435 kb on - strand, within Psyr_4687 at 5555.435 kb on - strand, within Psyr_4687 at 5555.435 kb on - strand, within Psyr_4687 at 5555.437 kb on + strand, within Psyr_4687 at 5555.438 kb on - strand, within Psyr_4687 at 5555.438 kb on - strand, within Psyr_4687 at 5555.548 kb on + strand, within Psyr_4687 at 5555.548 kb on + strand, within Psyr_4687 at 5555.549 kb on - strand, within Psyr_4687 at 5555.549 kb on - strand, within Psyr_4687 at 5555.549 kb on - strand, within Psyr_4687 at 5555.549 kb on - strand, within Psyr_4687 at 5555.686 kb on + strand, within Psyr_4687 at 5555.687 kb on - strand, within Psyr_4687 at 5555.707 kb on + strand, within Psyr_4687 at 5555.708 kb on - strand, within Psyr_4687 at 5555.708 kb on - strand, within Psyr_4687 at 5555.708 kb on - strand, within Psyr_4687
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 5,552,630 + Psyr_4684 0.70 -2.3 5,552,630 + Psyr_4684 0.70 +0.3 5,552,631 - Psyr_4684 0.70 +1.3 5,552,782 + Psyr_4684 0.89 +0.3 5,553,060 - Psyr_4685 0.27 +2.3 5,553,097 + Psyr_4685 0.32 -3.6 5,553,098 - Psyr_4685 0.32 +0.3 5,553,656 + -0.3 5,553,659 + +0.3 5,553,659 + +0.3 5,553,659 + +3.4 5,553,659 + -4.6 5,553,660 - +0.3 5,553,660 - +0.3 5,553,660 - +1.3 5,553,987 + Psyr_4686 0.34 -2.1 5,553,987 + Psyr_4686 0.34 +0.3 5,553,987 + Psyr_4686 0.34 +0.3 5,553,987 + Psyr_4686 0.34 -3.1 5,553,987 + Psyr_4686 0.34 -1.7 5,553,988 - Psyr_4686 0.34 +0.3 5,553,988 - Psyr_4686 0.34 -3.2 5,553,988 - Psyr_4686 0.34 -3.4 5,553,988 - Psyr_4686 0.34 +2.6 5,553,993 + Psyr_4686 0.34 +1.3 5,553,993 + Psyr_4686 0.34 -4.1 5,553,994 - Psyr_4686 0.34 -3.1 5,554,458 + Psyr_4686 0.73 +0.3 5,554,458 + Psyr_4686 0.73 -2.1 5,554,458 + Psyr_4686 0.73 +0.3 5,554,459 - Psyr_4686 0.73 -1.5 5,554,459 - Psyr_4686 0.73 +2.6 5,554,459 - Psyr_4686 0.73 -1.7 5,554,459 - Psyr_4686 0.73 +0.3 5,554,638 + Psyr_4686 0.88 +0.3 5,554,638 + Psyr_4686 0.88 -2.5 5,554,638 + Psyr_4686 0.88 +0.3 5,554,639 - Psyr_4686 0.88 -3.2 5,554,639 - Psyr_4686 0.88 +2.3 5,554,639 - Psyr_4686 0.88 +0.3 5,554,639 - Psyr_4686 0.88 -2.6 5,554,861 + -3.6 5,554,861 + +0.3 5,554,861 + +0.3 5,554,861 + -4.0 5,554,862 - -0.6 5,554,862 - +0.3 5,554,862 - +3.3 5,554,862 - -1.7 5,555,026 + Psyr_4687 0.17 +0.3 5,555,129 + Psyr_4687 0.27 +0.3 5,555,129 + Psyr_4687 0.27 +0.3 5,555,129 + +0.3 5,555,129 + Psyr_4687 0.27 +0.3 5,555,130 - Psyr_4687 0.27 -2.7 5,555,130 - Psyr_4687 0.27 -3.2 5,555,130 - Psyr_4687 0.27 +1.3 5,555,130 - Psyr_4687 0.27 -3.0 5,555,347 + Psyr_4687 0.48 -3.7 5,555,347 + Psyr_4687 0.48 +0.3 5,555,347 + Psyr_4687 0.48 -3.3 5,555,347 + Psyr_4687 0.48 -1.3 5,555,347 + Psyr_4687 0.48 +0.3 5,555,347 + Psyr_4687 0.48 +0.3 5,555,348 - Psyr_4687 0.48 +1.8 5,555,348 - Psyr_4687 0.48 -4.0 5,555,348 - Psyr_4687 0.48 +0.3 5,555,348 - Psyr_4687 0.48 +1.8 5,555,348 - Psyr_4687 0.48 +0.3 5,555,348 - Psyr_4687 0.48 -5.0 5,555,374 + Psyr_4687 0.50 +2.6 5,555,390 + Psyr_4687 0.52 -2.9 5,555,390 + Psyr_4687 0.52 +0.3 5,555,391 - Psyr_4687 0.52 -4.3 5,555,434 + Psyr_4687 0.56 -3.7 5,555,434 + -2.6 5,555,434 + Psyr_4687 0.56 -3.1 5,555,434 + Psyr_4687 0.56 +0.3 5,555,434 + Psyr_4687 0.56 -3.1 5,555,434 + Psyr_4687 0.56 +0.3 5,555,434 + Psyr_4687 0.56 +2.3 5,555,435 - Psyr_4687 0.56 -4.4 5,555,435 - Psyr_4687 0.56 -3.4 5,555,435 - Psyr_4687 0.56 -3.0 5,555,435 - Psyr_4687 0.56 -3.7 5,555,437 + Psyr_4687 0.56 +0.3 5,555,438 - Psyr_4687 0.56 +0.3 5,555,438 - Psyr_4687 0.56 +0.3 5,555,548 + Psyr_4687 0.67 +0.3 5,555,548 + Psyr_4687 0.67 +2.3 5,555,549 - Psyr_4687 0.67 +0.3 5,555,549 - Psyr_4687 0.67 +0.3 5,555,549 - Psyr_4687 0.67 +1.3 5,555,549 - Psyr_4687 0.67 +0.3 5,555,686 + Psyr_4687 0.80 -3.8 5,555,687 - Psyr_4687 0.80 +0.3 5,555,707 + Psyr_4687 0.82 +0.3 5,555,708 - Psyr_4687 0.82 -3.8 5,555,708 - Psyr_4687 0.82 -2.9 5,555,708 - Psyr_4687 0.82 +0.3
Or see this region's nucleotide sequence