Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4681 and Psyr_4682 are separated by 333 nucleotides Psyr_4682 and Psyr_4683 are separated by 153 nucleotides Psyr_4683 and Psyr_4684 are separated by 24 nucleotides Psyr_4684 and Psyr_4685 overlap by 8 nucleotides
Psyr_4681: Psyr_4681 - Acyl-CoA dehydrogenase, C-terminal:Acyl-CoA dehydrogenase, central region, at 5,548,767 to 5,550,572
_4681
Psyr_4682: Psyr_4682 - conserved hypothetical protein, at 5,550,906 to 5,551,202
_4682
Psyr_4683: Psyr_4683 - dethiobiotin synthase, at 5,551,356 to 5,552,036
_4683
Psyr_4684: Psyr_4684 - biotin synthesis protein BioC, at 5,552,061 to 5,552,870
_4684
Psyr_4685: Psyr_4685 - bioH protein, at 5,552,863 to 5,553,594
_4685
Position (kb) 5551
5552
5553 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4
5 at 5550.512 kb on - strand at 5550.562 kb on + strand at 5550.562 kb on + strand at 5550.562 kb on + strand at 5550.562 kb on + strand at 5550.562 kb on + strand at 5550.562 kb on + strand at 5550.563 kb on - strand at 5550.563 kb on - strand at 5550.563 kb on - strand at 5550.572 kb on + strand at 5550.573 kb on - strand at 5550.655 kb on + strand at 5550.655 kb on + strand at 5550.655 kb on + strand at 5550.655 kb on + strand at 5550.655 kb on + strand at 5550.655 kb on + strand at 5550.655 kb on + strand at 5550.656 kb on - strand at 5550.656 kb on - strand at 5550.687 kb on + strand at 5550.687 kb on + strand at 5550.693 kb on + strand at 5550.713 kb on - strand at 5550.713 kb on - strand at 5550.748 kb on + strand at 5550.749 kb on - strand at 5550.763 kb on + strand at 5550.763 kb on + strand at 5550.763 kb on + strand at 5550.764 kb on - strand at 5550.764 kb on - strand at 5550.789 kb on + strand at 5550.801 kb on + strand at 5550.913 kb on + strand at 5550.913 kb on + strand at 5550.914 kb on - strand at 5550.914 kb on - strand at 5550.914 kb on - strand at 5550.914 kb on - strand at 5550.914 kb on - strand at 5550.914 kb on - strand at 5551.007 kb on + strand, within Psyr_4682 at 5551.007 kb on + strand, within Psyr_4682 at 5551.008 kb on - strand, within Psyr_4682 at 5551.202 kb on + strand at 5551.202 kb on + strand at 5551.263 kb on - strand at 5551.278 kb on + strand at 5551.345 kb on + strand at 5551.381 kb on + strand at 5551.382 kb on - strand at 5551.530 kb on + strand, within Psyr_4683 at 5551.530 kb on + strand, within Psyr_4683 at 5551.531 kb on - strand, within Psyr_4683 at 5551.615 kb on - strand, within Psyr_4683 at 5551.721 kb on + strand, within Psyr_4683 at 5551.721 kb on + strand, within Psyr_4683 at 5551.721 kb on + strand, within Psyr_4683 at 5551.721 kb on + strand, within Psyr_4683 at 5551.721 kb on + strand, within Psyr_4683 at 5551.721 kb on + strand, within Psyr_4683 at 5551.722 kb on - strand, within Psyr_4683 at 5551.722 kb on - strand, within Psyr_4683 at 5551.722 kb on - strand, within Psyr_4683 at 5551.722 kb on - strand, within Psyr_4683 at 5551.722 kb on - strand, within Psyr_4683 at 5551.831 kb on + strand, within Psyr_4683 at 5552.083 kb on + strand at 5552.084 kb on - strand at 5552.095 kb on + strand at 5552.095 kb on + strand at 5552.095 kb on + strand at 5552.095 kb on + strand at 5552.096 kb on - strand at 5552.096 kb on - strand at 5552.096 kb on - strand at 5552.096 kb on - strand at 5552.134 kb on + strand at 5552.135 kb on - strand at 5552.135 kb on - strand at 5552.135 kb on - strand at 5552.135 kb on - strand at 5552.392 kb on + strand, within Psyr_4684 at 5552.392 kb on + strand, within Psyr_4684 at 5552.393 kb on - strand, within Psyr_4684 at 5552.630 kb on + strand, within Psyr_4684 at 5552.630 kb on + strand, within Psyr_4684 at 5552.631 kb on - strand, within Psyr_4684 at 5552.782 kb on + strand, within Psyr_4684
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 5,550,512 - +1.6 5,550,562 + -4.1 5,550,562 + -0.9 5,550,562 + -1.1 5,550,562 + +3.7 5,550,562 + +0.6 5,550,562 + +1.8 5,550,563 - +0.4 5,550,563 - -1.3 5,550,563 - +2.6 5,550,572 + -0.0 5,550,573 - -1.4 5,550,655 + +5.3 5,550,655 + +0.2 5,550,655 + +0.7 5,550,655 + +1.8 5,550,655 + +0.3 5,550,655 + +1.3 5,550,655 + +2.3 5,550,656 - -0.1 5,550,656 - -2.2 5,550,687 + -1.2 5,550,687 + +1.8 5,550,693 + +0.3 5,550,713 - +1.8 5,550,713 - +3.3 5,550,748 + -0.8 5,550,749 - -0.1 5,550,763 + -0.7 5,550,763 + -0.9 5,550,763 + -0.2 5,550,764 - -4.4 5,550,764 - -2.0 5,550,789 + +1.0 5,550,801 + +0.3 5,550,913 + -1.6 5,550,913 + -0.6 5,550,914 - +4.0 5,550,914 - +1.3 5,550,914 - +4.0 5,550,914 - +3.3 5,550,914 - -2.2 5,550,914 - -0.5 5,551,007 + Psyr_4682 0.34 +1.0 5,551,007 + Psyr_4682 0.34 -2.2 5,551,008 - Psyr_4682 0.34 +3.2 5,551,202 + +2.3 5,551,202 + -1.6 5,551,263 - -0.3 5,551,278 + -0.8 5,551,345 + -1.1 5,551,381 + +0.3 5,551,382 - +0.3 5,551,530 + Psyr_4683 0.26 -3.2 5,551,530 + Psyr_4683 0.26 +0.3 5,551,531 - Psyr_4683 0.26 +0.3 5,551,615 - Psyr_4683 0.38 -1.2 5,551,721 + Psyr_4683 0.54 -3.1 5,551,721 + Psyr_4683 0.54 +0.3 5,551,721 + Psyr_4683 0.54 -1.9 5,551,721 + Psyr_4683 0.54 +0.3 5,551,721 + Psyr_4683 0.54 +0.3 5,551,721 + Psyr_4683 0.54 -2.2 5,551,722 - Psyr_4683 0.54 -5.0 5,551,722 - Psyr_4683 0.54 +1.3 5,551,722 - Psyr_4683 0.54 -5.0 5,551,722 - Psyr_4683 0.54 -3.8 5,551,722 - Psyr_4683 0.54 +2.3 5,551,831 + Psyr_4683 0.70 -4.3 5,552,083 + +1.3 5,552,084 - +0.3 5,552,095 + -1.8 5,552,095 + +0.3 5,552,095 + +0.3 5,552,095 + -3.2 5,552,096 - +1.3 5,552,096 - +0.3 5,552,096 - -3.9 5,552,096 - +0.3 5,552,134 + -0.7 5,552,135 - +1.3 5,552,135 - -1.7 5,552,135 - +0.3 5,552,135 - -3.6 5,552,392 + Psyr_4684 0.41 +0.3 5,552,392 + Psyr_4684 0.41 -4.8 5,552,393 - Psyr_4684 0.41 +3.7 5,552,630 + Psyr_4684 0.70 -2.3 5,552,630 + Psyr_4684 0.70 +0.3 5,552,631 - Psyr_4684 0.70 +1.3 5,552,782 + Psyr_4684 0.89 +0.3
Or see this region's nucleotide sequence