Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4566 and Psyr_4567 are separated by 3 nucleotides Psyr_4567 and Psyr_4568 are separated by 62 nucleotides Psyr_4568 and Psyr_4569 are separated by 264 nucleotides
Psyr_4566: Psyr_4566 - Peptidase M23B, at 5,430,185 to 5,431,612
_4566
Psyr_4567: Psyr_4567 - Protein of unknown function UPF0075, at 5,431,616 to 5,432,710
_4567
Psyr_4568: Psyr_4568 - HesB/YadR/YfhF, at 5,432,773 to 5,433,123
_4568
Psyr_4569: Psyr_4569 - N-acetyl-gamma-glutamyl-phosphate reductase, at 5,433,388 to 5,434,422
_4569
Position (kb) 5431
5432
5433 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3
4 at 5430.752 kb on + strand, within Psyr_4566 at 5430.797 kb on + strand, within Psyr_4566 at 5430.801 kb on + strand, within Psyr_4566 at 5430.801 kb on + strand, within Psyr_4566 at 5430.801 kb on + strand, within Psyr_4566 at 5430.801 kb on + strand, within Psyr_4566 at 5430.801 kb on + strand, within Psyr_4566 at 5430.802 kb on - strand, within Psyr_4566 at 5430.802 kb on - strand, within Psyr_4566 at 5430.802 kb on - strand, within Psyr_4566 at 5430.802 kb on - strand, within Psyr_4566 at 5430.802 kb on - strand, within Psyr_4566 at 5430.802 kb on - strand, within Psyr_4566 at 5430.802 kb on - strand, within Psyr_4566 at 5430.859 kb on + strand, within Psyr_4566 at 5430.859 kb on + strand, within Psyr_4566 at 5430.859 kb on + strand, within Psyr_4566 at 5430.859 kb on + strand, within Psyr_4566 at 5430.859 kb on + strand, within Psyr_4566 at 5430.860 kb on - strand, within Psyr_4566 at 5430.860 kb on - strand, within Psyr_4566 at 5430.860 kb on - strand, within Psyr_4566 at 5430.860 kb on - strand, within Psyr_4566 at 5430.945 kb on + strand, within Psyr_4566 at 5430.945 kb on + strand, within Psyr_4566 at 5430.945 kb on + strand, within Psyr_4566 at 5430.945 kb on + strand, within Psyr_4566 at 5430.946 kb on - strand, within Psyr_4566 at 5430.947 kb on + strand, within Psyr_4566 at 5430.948 kb on - strand, within Psyr_4566 at 5430.948 kb on - strand, within Psyr_4566 at 5430.948 kb on - strand, within Psyr_4566 at 5430.948 kb on - strand, within Psyr_4566 at 5430.948 kb on - strand, within Psyr_4566 at 5430.948 kb on - strand, within Psyr_4566 at 5430.979 kb on - strand, within Psyr_4566 at 5430.979 kb on - strand, within Psyr_4566 at 5430.991 kb on + strand, within Psyr_4566 at 5431.028 kb on + strand, within Psyr_4566 at 5431.028 kb on + strand, within Psyr_4566 at 5431.029 kb on - strand, within Psyr_4566 at 5431.029 kb on - strand, within Psyr_4566 at 5431.029 kb on - strand, within Psyr_4566 at 5431.043 kb on + strand, within Psyr_4566 at 5431.064 kb on - strand, within Psyr_4566 at 5431.070 kb on + strand, within Psyr_4566 at 5431.070 kb on + strand, within Psyr_4566 at 5431.071 kb on - strand, within Psyr_4566 at 5431.073 kb on + strand, within Psyr_4566 at 5431.074 kb on - strand, within Psyr_4566 at 5431.074 kb on - strand, within Psyr_4566 at 5431.074 kb on - strand, within Psyr_4566 at 5431.074 kb on - strand, within Psyr_4566 at 5431.208 kb on + strand, within Psyr_4566 at 5431.208 kb on + strand, within Psyr_4566 at 5431.319 kb on + strand, within Psyr_4566 at 5431.319 kb on + strand, within Psyr_4566 at 5431.320 kb on - strand, within Psyr_4566 at 5431.320 kb on - strand, within Psyr_4566 at 5431.320 kb on - strand, within Psyr_4566 at 5431.324 kb on + strand, within Psyr_4566 at 5431.325 kb on - strand, within Psyr_4566 at 5431.331 kb on + strand, within Psyr_4566 at 5431.331 kb on + strand, within Psyr_4566 at 5431.331 kb on + strand, within Psyr_4566 at 5431.331 kb on + strand, within Psyr_4566 at 5431.332 kb on - strand, within Psyr_4566 at 5431.332 kb on - strand, within Psyr_4566 at 5431.332 kb on - strand, within Psyr_4566 at 5431.403 kb on + strand, within Psyr_4566 at 5431.404 kb on - strand, within Psyr_4566 at 5431.405 kb on + strand, within Psyr_4566 at 5431.414 kb on + strand, within Psyr_4566 at 5431.446 kb on - strand, within Psyr_4566 at 5431.473 kb on + strand at 5431.473 kb on + strand at 5431.603 kb on + strand at 5431.604 kb on - strand at 5431.628 kb on + strand at 5431.628 kb on + strand at 5431.628 kb on + strand at 5431.628 kb on + strand at 5431.628 kb on + strand at 5431.628 kb on + strand at 5431.628 kb on + strand at 5431.628 kb on + strand at 5431.628 kb on + strand at 5431.628 kb on + strand at 5431.628 kb on + strand at 5431.628 kb on + strand at 5431.628 kb on + strand at 5431.628 kb on + strand at 5431.628 kb on + strand at 5431.629 kb on - strand at 5431.629 kb on - strand at 5431.629 kb on - strand at 5431.629 kb on - strand at 5431.629 kb on - strand at 5431.629 kb on - strand at 5431.629 kb on - strand at 5431.629 kb on - strand at 5431.632 kb on + strand at 5431.632 kb on + strand at 5431.632 kb on + strand at 5431.632 kb on + strand at 5431.632 kb on + strand at 5431.632 kb on + strand at 5431.632 kb on + strand at 5431.632 kb on + strand at 5431.632 kb on + strand at 5431.632 kb on + strand at 5431.632 kb on + strand at 5431.632 kb on + strand at 5431.632 kb on + strand at 5431.632 kb on + strand at 5431.632 kb on + strand at 5431.632 kb on + strand at 5431.632 kb on + strand at 5431.632 kb on + strand at 5431.632 kb on + strand at 5431.632 kb on + strand at 5431.632 kb on + strand at 5431.633 kb on - strand at 5431.633 kb on - strand at 5431.633 kb on - strand at 5431.633 kb on - strand at 5431.633 kb on - strand at 5431.633 kb on - strand at 5431.633 kb on - strand at 5431.633 kb on - strand at 5431.633 kb on - strand at 5431.633 kb on - strand at 5431.633 kb on - strand at 5431.633 kb on - strand at 5431.633 kb on - strand at 5431.633 kb on - strand at 5431.633 kb on - strand at 5431.633 kb on - strand at 5431.633 kb on - strand at 5431.633 kb on - strand at 5431.633 kb on - strand at 5431.633 kb on - strand at 5431.633 kb on - strand at 5431.633 kb on - strand at 5431.633 kb on - strand at 5431.717 kb on + strand at 5431.718 kb on + strand at 5431.718 kb on + strand at 5431.718 kb on + strand at 5431.718 kb on + strand at 5431.718 kb on + strand at 5431.719 kb on - strand at 5431.719 kb on - strand at 5431.719 kb on - strand at 5431.719 kb on - strand at 5431.719 kb on - strand at 5431.719 kb on - strand at 5431.719 kb on - strand at 5431.719 kb on - strand at 5431.719 kb on - strand at 5431.719 kb on - strand at 5431.719 kb on - strand at 5431.909 kb on + strand, within Psyr_4567 at 5431.910 kb on - strand, within Psyr_4567 at 5431.910 kb on - strand, within Psyr_4567 at 5431.910 kb on - strand, within Psyr_4567 at 5432.181 kb on + strand, within Psyr_4567 at 5432.181 kb on + strand, within Psyr_4567 at 5432.182 kb on - strand, within Psyr_4567 at 5432.182 kb on - strand, within Psyr_4567 at 5432.182 kb on - strand, within Psyr_4567 at 5432.222 kb on + strand, within Psyr_4567 at 5432.222 kb on + strand, within Psyr_4567 at 5432.223 kb on - strand, within Psyr_4567 at 5432.324 kb on - strand, within Psyr_4567 at 5432.324 kb on - strand, within Psyr_4567 at 5432.545 kb on + strand, within Psyr_4567 at 5433.274 kb on + strand at 5433.274 kb on + strand at 5433.281 kb on + strand at 5433.282 kb on - strand at 5433.282 kb on - strand at 5433.282 kb on - strand at 5433.282 kb on - strand at 5433.282 kb on - strand at 5433.282 kb on - strand at 5433.282 kb on - strand at 5433.282 kb on - strand at 5433.282 kb on - strand at 5433.288 kb on + strand at 5433.288 kb on + strand at 5433.288 kb on + strand at 5433.288 kb on + strand at 5433.288 kb on + strand at 5433.288 kb on + strand at 5433.289 kb on - strand at 5433.295 kb on + strand at 5433.295 kb on + strand at 5433.295 kb on + strand at 5433.296 kb on - strand at 5433.296 kb on - strand at 5433.296 kb on - strand at 5433.296 kb on - strand at 5433.296 kb on - strand at 5433.296 kb on - strand at 5433.296 kb on - strand at 5433.302 kb on + strand at 5433.302 kb on + strand at 5433.302 kb on + strand at 5433.302 kb on + strand at 5433.303 kb on - strand at 5433.303 kb on - strand at 5433.309 kb on + strand at 5433.309 kb on + strand at 5433.309 kb on + strand at 5433.309 kb on + strand at 5433.309 kb on + strand at 5433.316 kb on + strand at 5433.316 kb on + strand at 5433.317 kb on - strand at 5433.317 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 5,430,752 + Psyr_4566 0.40 +0.2 5,430,797 + Psyr_4566 0.43 +1.2 5,430,801 + Psyr_4566 0.43 +0.2 5,430,801 + Psyr_4566 0.43 +0.2 5,430,801 + Psyr_4566 0.43 +0.2 5,430,801 + Psyr_4566 0.43 -3.0 5,430,801 + Psyr_4566 0.43 -3.1 5,430,802 - Psyr_4566 0.43 +0.2 5,430,802 - Psyr_4566 0.43 +0.2 5,430,802 - Psyr_4566 0.43 -3.9 5,430,802 - Psyr_4566 0.43 -3.7 5,430,802 - Psyr_4566 0.43 -4.5 5,430,802 - Psyr_4566 0.43 +0.2 5,430,802 - Psyr_4566 0.43 -3.7 5,430,859 + Psyr_4566 0.47 +0.2 5,430,859 + Psyr_4566 0.47 -5.9 5,430,859 + Psyr_4566 0.47 +0.2 5,430,859 + Psyr_4566 0.47 +0.2 5,430,859 + Psyr_4566 0.47 -2.9 5,430,860 - Psyr_4566 0.47 +0.2 5,430,860 - Psyr_4566 0.47 +2.2 5,430,860 - Psyr_4566 0.47 -3.6 5,430,860 - Psyr_4566 0.47 +0.2 5,430,945 + Psyr_4566 0.53 -3.4 5,430,945 + Psyr_4566 0.53 -4.9 5,430,945 + Psyr_4566 0.53 +2.2 5,430,945 + Psyr_4566 0.53 -3.1 5,430,946 - Psyr_4566 0.53 +0.2 5,430,947 + Psyr_4566 0.53 +1.2 5,430,948 - Psyr_4566 0.53 -2.4 5,430,948 - Psyr_4566 0.53 -2.6 5,430,948 - Psyr_4566 0.53 +0.2 5,430,948 - Psyr_4566 0.53 +0.7 5,430,948 - Psyr_4566 0.53 -3.0 5,430,948 - Psyr_4566 0.53 +2.2 5,430,979 - Psyr_4566 0.56 +0.2 5,430,979 - Psyr_4566 0.56 +0.2 5,430,991 + Psyr_4566 0.56 +0.2 5,431,028 + Psyr_4566 0.59 +0.2 5,431,028 + Psyr_4566 0.59 -4.5 5,431,029 - Psyr_4566 0.59 +0.2 5,431,029 - Psyr_4566 0.59 -4.5 5,431,029 - Psyr_4566 0.59 +0.2 5,431,043 + Psyr_4566 0.60 +0.2 5,431,064 - Psyr_4566 0.62 +0.2 5,431,070 + Psyr_4566 0.62 -1.4 5,431,070 + Psyr_4566 0.62 -3.2 5,431,071 - Psyr_4566 0.62 +1.2 5,431,073 + Psyr_4566 0.62 -4.4 5,431,074 - Psyr_4566 0.62 -3.4 5,431,074 - Psyr_4566 0.62 +1.2 5,431,074 - Psyr_4566 0.62 +0.2 5,431,074 - Psyr_4566 0.62 +0.2 5,431,208 + Psyr_4566 0.72 +0.2 5,431,208 + Psyr_4566 0.72 -3.9 5,431,319 + Psyr_4566 0.79 -4.1 5,431,319 + Psyr_4566 0.79 +2.2 5,431,320 - Psyr_4566 0.79 +0.2 5,431,320 - Psyr_4566 0.79 -0.7 5,431,320 - Psyr_4566 0.79 -2.6 5,431,324 + Psyr_4566 0.80 -3.8 5,431,325 - Psyr_4566 0.80 +0.2 5,431,331 + Psyr_4566 0.80 -2.6 5,431,331 + Psyr_4566 0.80 -2.3 5,431,331 + Psyr_4566 0.80 -0.4 5,431,331 + Psyr_4566 0.80 +0.2 5,431,332 - Psyr_4566 0.80 -2.8 5,431,332 - Psyr_4566 0.80 +1.8 5,431,332 - Psyr_4566 0.80 -3.5 5,431,403 + Psyr_4566 0.85 +0.2 5,431,404 - Psyr_4566 0.85 -3.2 5,431,405 + Psyr_4566 0.85 +1.8 5,431,414 + Psyr_4566 0.86 +0.2 5,431,446 - Psyr_4566 0.88 +0.2 5,431,473 + -0.8 5,431,473 + +0.2 5,431,603 + -1.9 5,431,604 - -4.1 5,431,628 + -3.9 5,431,628 + -1.4 5,431,628 + -1.9 5,431,628 + +0.2 5,431,628 + +1.8 5,431,628 + +0.6 5,431,628 + -2.9 5,431,628 + -0.9 5,431,628 + +0.2 5,431,628 + +0.2 5,431,628 + -3.7 5,431,628 + +2.8 5,431,628 + -0.4 5,431,628 + -2.1 5,431,628 + -0.9 5,431,629 - -1.4 5,431,629 - +1.8 5,431,629 - +2.2 5,431,629 - -2.6 5,431,629 - -1.5 5,431,629 - -2.2 5,431,629 - -1.0 5,431,629 - -2.4 5,431,632 + +1.2 5,431,632 + -5.2 5,431,632 + -3.9 5,431,632 + -0.8 5,431,632 + -3.5 5,431,632 + +1.3 5,431,632 + -3.5 5,431,632 + -0.7 5,431,632 + -1.2 5,431,632 + +3.4 5,431,632 + -1.4 5,431,632 + -2.1 5,431,632 + +1.2 5,431,632 + -4.5 5,431,632 + -2.0 5,431,632 + -0.4 5,431,632 + -3.1 5,431,632 + +0.2 5,431,632 + +0.5 5,431,632 + +2.5 5,431,632 + -1.4 5,431,633 - -2.0 5,431,633 - -0.8 5,431,633 - -2.1 5,431,633 - -1.4 5,431,633 - -0.1 5,431,633 - +0.1 5,431,633 - +1.8 5,431,633 - -3.0 5,431,633 - +2.5 5,431,633 - -2.6 5,431,633 - -3.6 5,431,633 - -1.7 5,431,633 - -0.5 5,431,633 - -0.6 5,431,633 - -1.6 5,431,633 - +1.8 5,431,633 - -0.0 5,431,633 - -1.5 5,431,633 - -0.4 5,431,633 - -1.6 5,431,633 - -2.2 5,431,633 - -1.2 5,431,633 - -4.1 5,431,717 + -4.9 5,431,718 + -0.2 5,431,718 + -0.5 5,431,718 + -1.2 5,431,718 + +3.5 5,431,718 + -1.2 5,431,719 - +2.6 5,431,719 - +2.5 5,431,719 - -2.9 5,431,719 - -1.1 5,431,719 - +3.2 5,431,719 - +1.2 5,431,719 - +1.8 5,431,719 - -0.0 5,431,719 - -4.7 5,431,719 - -4.0 5,431,719 - -0.7 5,431,909 + Psyr_4567 0.27 -3.1 5,431,910 - Psyr_4567 0.27 +2.2 5,431,910 - Psyr_4567 0.27 +2.3 5,431,910 - Psyr_4567 0.27 +2.2 5,432,181 + Psyr_4567 0.52 -3.6 5,432,181 + Psyr_4567 0.52 +2.8 5,432,182 - Psyr_4567 0.52 -1.1 5,432,182 - Psyr_4567 0.52 +2.5 5,432,182 - Psyr_4567 0.52 -3.7 5,432,222 + Psyr_4567 0.55 -1.5 5,432,222 + Psyr_4567 0.55 -1.0 5,432,223 - Psyr_4567 0.55 +1.8 5,432,324 - Psyr_4567 0.65 -4.1 5,432,324 - Psyr_4567 0.65 -1.1 5,432,545 + Psyr_4567 0.85 -3.5 5,433,274 + -2.2 5,433,274 + -1.3 5,433,281 + +2.8 5,433,282 - -0.8 5,433,282 - +1.0 5,433,282 - +3.0 5,433,282 - -2.0 5,433,282 - +2.5 5,433,282 - -0.9 5,433,282 - +3.5 5,433,282 - -1.3 5,433,282 - +1.2 5,433,288 + +2.8 5,433,288 + -0.2 5,433,288 + -2.8 5,433,288 + +3.2 5,433,288 + +0.0 5,433,288 + +1.2 5,433,289 - +3.0 5,433,295 + -1.7 5,433,295 + -0.4 5,433,295 + +2.8 5,433,296 - +0.3 5,433,296 - +3.9 5,433,296 - -1.2 5,433,296 - +0.2 5,433,296 - +0.8 5,433,296 - +3.5 5,433,296 - -1.2 5,433,302 + -1.5 5,433,302 + +1.8 5,433,302 + -0.0 5,433,302 + +2.4 5,433,303 - -0.5 5,433,303 - -2.2 5,433,309 + +1.4 5,433,309 + -0.2 5,433,309 + -2.0 5,433,309 + +3.8 5,433,309 + +3.2 5,433,316 + +0.6 5,433,316 + -2.7 5,433,317 - +3.2 5,433,317 - +1.5
Or see this region's nucleotide sequence