Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4414 and Psyr_4415 overlap by 1 nucleotides Psyr_4415 and Psyr_4416 overlap by 4 nucleotides Psyr_4416 and Psyr_4417 overlap by 8 nucleotides
Psyr_4414: Psyr_4414 - precorrin-3 methyltransferase, at 5,277,564 to 5,279,267
_4414
Psyr_4415: Psyr_4415 - cobalt-factor II C20-methyltransferase / precorrin-2 C20-methyltransferase, at 5,279,267 to 5,280,001
_4415
Psyr_4416: Psyr_4416 - precorrin-8X methylmutase, at 5,279,998 to 5,280,624
_4416
Psyr_4417: Psyr_4417 - Ferredoxin--nitrite reductase, at 5,280,617 to 5,281,960
_4417
Position (kb) 5279
5280
5281 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 5279.101 kb on + strand at 5279.102 kb on - strand at 5279.102 kb on - strand at 5279.134 kb on + strand at 5279.134 kb on + strand at 5279.135 kb on - strand at 5279.135 kb on - strand at 5279.135 kb on - strand at 5279.135 kb on - strand at 5279.297 kb on - strand at 5279.382 kb on + strand, within Psyr_4415 at 5279.382 kb on + strand, within Psyr_4415 at 5279.382 kb on + strand, within Psyr_4415 at 5279.382 kb on + strand, within Psyr_4415 at 5279.382 kb on + strand, within Psyr_4415 at 5279.383 kb on - strand, within Psyr_4415 at 5279.383 kb on - strand, within Psyr_4415 at 5279.616 kb on + strand, within Psyr_4415 at 5279.617 kb on - strand, within Psyr_4415 at 5279.617 kb on - strand, within Psyr_4415 at 5279.617 kb on - strand, within Psyr_4415 at 5279.643 kb on + strand, within Psyr_4415 at 5279.643 kb on + strand, within Psyr_4415 at 5279.643 kb on + strand, within Psyr_4415 at 5279.643 kb on + strand, within Psyr_4415 at 5279.643 kb on + strand, within Psyr_4415 at 5279.643 kb on + strand, within Psyr_4415 at 5279.643 kb on + strand, within Psyr_4415 at 5279.643 kb on + strand, within Psyr_4415 at 5279.644 kb on - strand, within Psyr_4415 at 5279.644 kb on - strand, within Psyr_4415 at 5279.644 kb on - strand, within Psyr_4415 at 5279.644 kb on - strand, within Psyr_4415 at 5279.649 kb on + strand, within Psyr_4415 at 5279.649 kb on + strand, within Psyr_4415 at 5279.649 kb on + strand, within Psyr_4415 at 5279.649 kb on + strand, within Psyr_4415 at 5279.649 kb on + strand, within Psyr_4415 at 5279.649 kb on + strand, within Psyr_4415 at 5279.649 kb on + strand, within Psyr_4415 at 5279.650 kb on - strand, within Psyr_4415 at 5279.650 kb on - strand, within Psyr_4415 at 5279.650 kb on - strand, within Psyr_4415 at 5279.650 kb on - strand, within Psyr_4415 at 5279.650 kb on - strand, within Psyr_4415 at 5279.650 kb on - strand, within Psyr_4415 at 5279.650 kb on - strand, within Psyr_4415 at 5279.650 kb on - strand, within Psyr_4415 at 5279.650 kb on - strand, within Psyr_4415 at 5279.650 kb on - strand, within Psyr_4415 at 5279.650 kb on - strand, within Psyr_4415 at 5279.659 kb on - strand, within Psyr_4415 at 5279.743 kb on - strand, within Psyr_4415 at 5279.743 kb on - strand, within Psyr_4415 at 5279.743 kb on - strand, within Psyr_4415 at 5279.875 kb on + strand, within Psyr_4415 at 5279.892 kb on + strand, within Psyr_4415 at 5279.892 kb on + strand, within Psyr_4415 at 5279.892 kb on + strand, within Psyr_4415 at 5279.893 kb on - strand, within Psyr_4415 at 5279.893 kb on - strand, within Psyr_4415 at 5279.893 kb on - strand, within Psyr_4415 at 5280.200 kb on + strand, within Psyr_4416 at 5280.200 kb on + strand, within Psyr_4416 at 5280.201 kb on - strand, within Psyr_4416 at 5280.201 kb on - strand, within Psyr_4416 at 5280.235 kb on + strand, within Psyr_4416 at 5280.235 kb on + strand, within Psyr_4416 at 5280.236 kb on - strand, within Psyr_4416 at 5280.236 kb on - strand, within Psyr_4416 at 5280.289 kb on - strand, within Psyr_4416 at 5280.590 kb on + strand at 5280.590 kb on + strand at 5280.590 kb on + strand at 5280.592 kb on + strand at 5280.592 kb on + strand at 5280.592 kb on + strand at 5280.593 kb on - strand at 5280.593 kb on - strand at 5280.593 kb on - strand at 5280.593 kb on - strand at 5280.593 kb on - strand at 5280.614 kb on + strand at 5280.614 kb on + strand at 5280.614 kb on + strand at 5280.615 kb on - strand at 5280.615 kb on - strand at 5280.673 kb on + strand at 5280.674 kb on - strand at 5280.674 kb on - strand at 5280.674 kb on - strand at 5280.674 kb on - strand at 5280.676 kb on + strand at 5280.676 kb on + strand at 5280.677 kb on - strand at 5280.677 kb on - strand at 5280.677 kb on - strand at 5280.730 kb on - strand at 5280.777 kb on - strand, within Psyr_4417 at 5280.820 kb on + strand, within Psyr_4417
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 5,279,101 + +2.8 5,279,102 - -1.2 5,279,102 - +4.2 5,279,134 + +2.2 5,279,134 + -0.6 5,279,135 - +3.0 5,279,135 - -2.0 5,279,135 - +0.7 5,279,135 - +0.2 5,279,297 - -2.2 5,279,382 + Psyr_4415 0.16 -0.8 5,279,382 + Psyr_4415 0.16 -0.2 5,279,382 + Psyr_4415 0.16 +0.5 5,279,382 + Psyr_4415 0.16 +3.5 5,279,382 + Psyr_4415 0.16 +0.8 5,279,383 - Psyr_4415 0.16 -0.1 5,279,383 - Psyr_4415 0.16 +3.0 5,279,616 + Psyr_4415 0.47 -0.2 5,279,617 - Psyr_4415 0.48 -1.4 5,279,617 - Psyr_4415 0.48 +3.0 5,279,617 - Psyr_4415 0.48 -3.0 5,279,643 + Psyr_4415 0.51 -0.2 5,279,643 + Psyr_4415 0.51 +2.2 5,279,643 + Psyr_4415 0.51 -1.1 5,279,643 + Psyr_4415 0.51 -0.1 5,279,643 + Psyr_4415 0.51 -1.7 5,279,643 + Psyr_4415 0.51 +0.2 5,279,643 + Psyr_4415 0.51 +4.4 5,279,643 + Psyr_4415 0.51 -3.8 5,279,644 - Psyr_4415 0.51 -1.6 5,279,644 - Psyr_4415 0.51 -0.6 5,279,644 - Psyr_4415 0.51 -1.2 5,279,644 - Psyr_4415 0.51 -2.1 5,279,649 + Psyr_4415 0.52 -2.3 5,279,649 + Psyr_4415 0.52 -2.3 5,279,649 + Psyr_4415 0.52 -1.5 5,279,649 + Psyr_4415 0.52 -0.3 5,279,649 + Psyr_4415 0.52 -0.1 5,279,649 + Psyr_4415 0.52 -2.0 5,279,649 + Psyr_4415 0.52 +2.2 5,279,650 - Psyr_4415 0.52 -1.0 5,279,650 - Psyr_4415 0.52 -1.0 5,279,650 - Psyr_4415 0.52 +3.7 5,279,650 - Psyr_4415 0.52 +3.0 5,279,650 - Psyr_4415 0.52 -0.8 5,279,650 - Psyr_4415 0.52 +2.5 5,279,650 - Psyr_4415 0.52 -1.4 5,279,650 - Psyr_4415 0.52 +2.2 5,279,650 - Psyr_4415 0.52 +1.8 5,279,650 - Psyr_4415 0.52 +3.5 5,279,650 - Psyr_4415 0.52 -3.1 5,279,659 - Psyr_4415 0.53 +0.8 5,279,743 - Psyr_4415 0.65 +1.2 5,279,743 - Psyr_4415 0.65 -2.6 5,279,743 - Psyr_4415 0.65 +0.6 5,279,875 + Psyr_4415 0.83 -2.6 5,279,892 + Psyr_4415 0.85 -0.9 5,279,892 + Psyr_4415 0.85 +3.2 5,279,892 + Psyr_4415 0.85 +1.2 5,279,893 - Psyr_4415 0.85 -3.3 5,279,893 - Psyr_4415 0.85 +1.8 5,279,893 - Psyr_4415 0.85 +3.8 5,280,200 + Psyr_4416 0.32 -0.9 5,280,200 + Psyr_4416 0.32 -1.0 5,280,201 - Psyr_4416 0.32 +3.5 5,280,201 - Psyr_4416 0.32 -2.8 5,280,235 + Psyr_4416 0.38 -2.9 5,280,235 + Psyr_4416 0.38 -0.9 5,280,236 - Psyr_4416 0.38 -1.4 5,280,236 - Psyr_4416 0.38 -1.1 5,280,289 - Psyr_4416 0.46 -0.2 5,280,590 + -0.3 5,280,590 + -1.4 5,280,590 + -1.2 5,280,592 + +3.2 5,280,592 + -1.4 5,280,592 + -1.5 5,280,593 - +2.2 5,280,593 - -1.7 5,280,593 - -1.3 5,280,593 - +1.8 5,280,593 - +3.0 5,280,614 + +3.7 5,280,614 + +0.2 5,280,614 + -0.6 5,280,615 - -2.6 5,280,615 - +0.7 5,280,673 + -2.0 5,280,674 - +1.2 5,280,674 - +5.1 5,280,674 - -2.5 5,280,674 - -0.6 5,280,676 + -0.3 5,280,676 + -3.1 5,280,677 - -2.9 5,280,677 - +3.4 5,280,677 - +0.2 5,280,730 - +1.8 5,280,777 - Psyr_4417 0.12 -1.2 5,280,820 + Psyr_4417 0.15 +3.5
Or see this region's nucleotide sequence