Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4411 and Psyr_4412 overlap by 8 nucleotides Psyr_4412 and Psyr_4413 are separated by 58 nucleotides Psyr_4413 and Psyr_4414 are separated by 348 nucleotides
Psyr_4411: Psyr_4411 - conserved hypothetical protein, at 5,275,015 to 5,275,650
_4411
Psyr_4412: Psyr_4412 - conserved hypothetical protein, at 5,275,643 to 5,276,341
_4412
Psyr_4413: Psyr_4413 - conserved hypothetical protein, at 5,276,400 to 5,277,215
_4413
Psyr_4414: Psyr_4414 - precorrin-3 methyltransferase, at 5,277,564 to 5,279,267
_4414
Position (kb)
5276
5277
5278 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 5275.565 kb on - strand, within Psyr_4411 at 5275.565 kb on - strand, within Psyr_4411 at 5275.731 kb on + strand, within Psyr_4412 at 5275.732 kb on - strand, within Psyr_4412 at 5275.732 kb on - strand, within Psyr_4412 at 5275.782 kb on + strand, within Psyr_4412 at 5275.782 kb on + strand, within Psyr_4412 at 5275.782 kb on + strand, within Psyr_4412 at 5275.786 kb on + strand, within Psyr_4412 at 5275.786 kb on + strand, within Psyr_4412 at 5275.786 kb on + strand, within Psyr_4412 at 5275.787 kb on - strand, within Psyr_4412 at 5275.787 kb on - strand, within Psyr_4412 at 5275.787 kb on - strand, within Psyr_4412 at 5275.787 kb on - strand, within Psyr_4412 at 5275.787 kb on - strand, within Psyr_4412 at 5275.947 kb on - strand, within Psyr_4412 at 5275.947 kb on - strand, within Psyr_4412 at 5276.235 kb on + strand, within Psyr_4412 at 5276.235 kb on + strand, within Psyr_4412 at 5276.235 kb on + strand, within Psyr_4412 at 5276.235 kb on + strand, within Psyr_4412 at 5276.235 kb on + strand, within Psyr_4412 at 5276.235 kb on + strand, within Psyr_4412 at 5276.235 kb on + strand, within Psyr_4412 at 5276.235 kb on + strand, within Psyr_4412 at 5276.235 kb on + strand, within Psyr_4412 at 5276.236 kb on - strand, within Psyr_4412 at 5276.236 kb on - strand, within Psyr_4412 at 5276.236 kb on - strand, within Psyr_4412 at 5276.236 kb on - strand, within Psyr_4412 at 5276.236 kb on - strand, within Psyr_4412 at 5276.236 kb on - strand, within Psyr_4412 at 5276.236 kb on - strand, within Psyr_4412 at 5276.236 kb on - strand, within Psyr_4412 at 5276.373 kb on + strand at 5276.380 kb on + strand at 5276.380 kb on + strand at 5276.380 kb on + strand at 5276.380 kb on + strand at 5276.380 kb on + strand at 5276.380 kb on + strand at 5276.381 kb on - strand at 5276.381 kb on - strand at 5276.381 kb on - strand at 5276.476 kb on + strand at 5276.477 kb on - strand at 5276.477 kb on - strand at 5276.477 kb on - strand at 5276.784 kb on - strand, within Psyr_4413 at 5276.784 kb on - strand, within Psyr_4413 at 5276.857 kb on + strand, within Psyr_4413 at 5276.857 kb on + strand, within Psyr_4413 at 5276.858 kb on - strand, within Psyr_4413 at 5276.858 kb on - strand, within Psyr_4413 at 5276.914 kb on + strand, within Psyr_4413 at 5276.914 kb on + strand, within Psyr_4413 at 5276.915 kb on - strand, within Psyr_4413 at 5277.014 kb on + strand, within Psyr_4413 at 5277.100 kb on - strand, within Psyr_4413 at 5277.125 kb on - strand, within Psyr_4413 at 5277.173 kb on - strand at 5277.173 kb on - strand at 5277.173 kb on - strand at 5277.173 kb on - strand at 5277.221 kb on + strand at 5277.229 kb on + strand at 5277.230 kb on - strand at 5277.230 kb on - strand at 5277.270 kb on + strand at 5277.384 kb on - strand at 5277.546 kb on + strand at 5277.547 kb on - strand at 5277.573 kb on - strand at 5277.586 kb on + strand at 5277.599 kb on + strand at 5277.601 kb on + strand at 5277.602 kb on - strand at 5277.602 kb on - strand at 5277.602 kb on - strand at 5277.841 kb on + strand, within Psyr_4414 at 5277.841 kb on + strand, within Psyr_4414 at 5277.842 kb on - strand, within Psyr_4414 at 5277.842 kb on - strand, within Psyr_4414
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 5,275,565 - Psyr_4411 0.86 +2.2 5,275,565 - Psyr_4411 0.86 +0.2 5,275,731 + Psyr_4412 0.13 -0.7 5,275,732 - Psyr_4412 0.13 +0.6 5,275,732 - Psyr_4412 0.13 +3.9 5,275,782 + Psyr_4412 0.20 +3.0 5,275,782 + Psyr_4412 0.20 -1.6 5,275,782 + Psyr_4412 0.20 +0.2 5,275,786 + Psyr_4412 0.20 +2.2 5,275,786 + Psyr_4412 0.20 -0.3 5,275,786 + Psyr_4412 0.20 -0.2 5,275,787 - Psyr_4412 0.21 +3.0 5,275,787 - Psyr_4412 0.21 -1.2 5,275,787 - Psyr_4412 0.21 -0.9 5,275,787 - Psyr_4412 0.21 +1.6 5,275,787 - Psyr_4412 0.21 +1.5 5,275,947 - Psyr_4412 0.43 -0.9 5,275,947 - Psyr_4412 0.43 -1.3 5,276,235 + Psyr_4412 0.85 -1.4 5,276,235 + Psyr_4412 0.85 +4.2 5,276,235 + Psyr_4412 0.85 +1.2 5,276,235 + Psyr_4412 0.85 -1.5 5,276,235 + Psyr_4412 0.85 +0.3 5,276,235 + Psyr_4412 0.85 -2.7 5,276,235 + Psyr_4412 0.85 -1.2 5,276,235 + Psyr_4412 0.85 +1.2 5,276,235 + Psyr_4412 0.85 +2.2 5,276,236 - Psyr_4412 0.85 -0.3 5,276,236 - Psyr_4412 0.85 +0.9 5,276,236 - Psyr_4412 0.85 -0.8 5,276,236 - Psyr_4412 0.85 +0.1 5,276,236 - Psyr_4412 0.85 +3.4 5,276,236 - Psyr_4412 0.85 -0.5 5,276,236 - Psyr_4412 0.85 -0.8 5,276,236 - Psyr_4412 0.85 -2.0 5,276,373 + +1.8 5,276,380 + -2.4 5,276,380 + +5.1 5,276,380 + +2.8 5,276,380 + -2.3 5,276,380 + -1.0 5,276,380 + -1.6 5,276,381 - -1.1 5,276,381 - -0.2 5,276,381 - +1.0 5,276,476 + +4.1 5,276,477 - +0.4 5,276,477 - -2.0 5,276,477 - -0.2 5,276,784 - Psyr_4413 0.47 -0.2 5,276,784 - Psyr_4413 0.47 +1.5 5,276,857 + Psyr_4413 0.56 +2.8 5,276,857 + Psyr_4413 0.56 +3.5 5,276,858 - Psyr_4413 0.56 -0.3 5,276,858 - Psyr_4413 0.56 +3.4 5,276,914 + Psyr_4413 0.63 -0.9 5,276,914 + Psyr_4413 0.63 +3.2 5,276,915 - Psyr_4413 0.63 -1.8 5,277,014 + Psyr_4413 0.75 -1.9 5,277,100 - Psyr_4413 0.86 +0.1 5,277,125 - Psyr_4413 0.89 -0.3 5,277,173 - +1.0 5,277,173 - +2.4 5,277,173 - +2.8 5,277,173 - -0.4 5,277,221 + -1.3 5,277,229 + +3.7 5,277,230 - +1.2 5,277,230 - +2.2 5,277,270 + -1.7 5,277,384 - -0.7 5,277,546 + -1.2 5,277,547 - -1.3 5,277,573 - +0.8 5,277,586 + +1.1 5,277,599 + +1.8 5,277,601 + +2.8 5,277,602 - -0.0 5,277,602 - -1.2 5,277,602 - +3.8 5,277,841 + Psyr_4414 0.16 +0.2 5,277,841 + Psyr_4414 0.16 +3.4 5,277,842 - Psyr_4414 0.16 -1.2 5,277,842 - Psyr_4414 0.16 +2.5
Or see this region's nucleotide sequence