Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4394 and Psyr_4395 are separated by 1 nucleotides Psyr_4395 and Psyr_4396 are separated by 35 nucleotides Psyr_4396 and Psyr_4397 overlap by 4 nucleotides Psyr_4397 and Psyr_4398 are separated by 76 nucleotides
Psyr_4394: Psyr_4394 - type II secretion system protein, at 5,256,440 to 5,257,324
_4394
Psyr_4395: Psyr_4395 - type II secretion system protein, at 5,257,326 to 5,258,210
_4395
Psyr_4396: Psyr_4396 - TPR repeat protein, at 5,258,246 to 5,258,980
_4396
Psyr_4397: Psyr_4397 - conserved hypothetical protein, at 5,258,977 to 5,259,249
_4397
Psyr_4398: Psyr_4398 - Protein-disulfide reductase, at 5,259,326 to 5,261,146
_4398
Position (kb) 5258
5259 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 5257.400 kb on + strand at 5257.400 kb on + strand at 5257.400 kb on + strand at 5257.401 kb on - strand at 5257.401 kb on - strand at 5257.401 kb on - strand at 5257.401 kb on - strand at 5257.401 kb on - strand at 5257.401 kb on - strand at 5257.401 kb on - strand at 5257.401 kb on - strand at 5257.403 kb on + strand at 5257.403 kb on + strand at 5257.404 kb on - strand at 5257.404 kb on - strand at 5257.404 kb on - strand at 5257.404 kb on - strand at 5257.535 kb on - strand, within Psyr_4395 at 5257.535 kb on - strand, within Psyr_4395 at 5257.535 kb on - strand, within Psyr_4395 at 5257.705 kb on - strand, within Psyr_4395 at 5257.705 kb on - strand, within Psyr_4395 at 5257.776 kb on - strand, within Psyr_4395 at 5258.091 kb on + strand, within Psyr_4395 at 5258.091 kb on + strand, within Psyr_4395 at 5258.091 kb on + strand, within Psyr_4395 at 5258.091 kb on + strand, within Psyr_4395 at 5258.092 kb on - strand, within Psyr_4395 at 5258.092 kb on - strand, within Psyr_4395 at 5258.092 kb on - strand, within Psyr_4395 at 5258.181 kb on - strand at 5258.181 kb on - strand at 5258.181 kb on - strand at 5258.188 kb on + strand at 5258.188 kb on + strand at 5258.189 kb on - strand at 5258.189 kb on - strand at 5258.189 kb on - strand at 5258.219 kb on + strand at 5258.219 kb on + strand at 5258.220 kb on - strand at 5258.220 kb on - strand at 5258.220 kb on - strand at 5258.220 kb on - strand at 5258.262 kb on + strand at 5258.262 kb on + strand at 5258.262 kb on + strand at 5258.263 kb on - strand at 5258.265 kb on + strand at 5258.265 kb on + strand at 5258.265 kb on + strand at 5258.265 kb on + strand at 5258.265 kb on + strand at 5258.266 kb on - strand at 5258.496 kb on + strand, within Psyr_4396 at 5258.497 kb on - strand, within Psyr_4396 at 5258.525 kb on + strand, within Psyr_4396 at 5258.687 kb on + strand, within Psyr_4396 at 5258.795 kb on + strand, within Psyr_4396 at 5258.795 kb on + strand, within Psyr_4396 at 5258.796 kb on - strand, within Psyr_4396 at 5258.796 kb on - strand, within Psyr_4396 at 5258.971 kb on - strand at 5258.971 kb on - strand at 5258.990 kb on + strand at 5258.990 kb on + strand at 5258.990 kb on + strand at 5258.991 kb on - strand at 5258.991 kb on - strand at 5258.991 kb on - strand at 5258.991 kb on - strand at 5258.991 kb on - strand at 5259.005 kb on + strand, within Psyr_4397 at 5259.005 kb on + strand, within Psyr_4397 at 5259.005 kb on + strand, within Psyr_4397 at 5259.005 kb on + strand, within Psyr_4397 at 5259.006 kb on - strand, within Psyr_4397 at 5259.006 kb on - strand, within Psyr_4397 at 5259.006 kb on - strand, within Psyr_4397 at 5259.181 kb on + strand, within Psyr_4397 at 5259.181 kb on + strand, within Psyr_4397 at 5259.181 kb on + strand, within Psyr_4397 at 5259.181 kb on + strand, within Psyr_4397 at 5259.181 kb on + strand, within Psyr_4397 at 5259.181 kb on + strand, within Psyr_4397 at 5259.181 kb on + strand, within Psyr_4397 at 5259.181 kb on + strand, within Psyr_4397 at 5259.181 kb on + strand, within Psyr_4397 at 5259.181 kb on + strand, within Psyr_4397 at 5259.181 kb on + strand, within Psyr_4397 at 5259.181 kb on + strand, within Psyr_4397 at 5259.181 kb on + strand, within Psyr_4397 at 5259.181 kb on + strand, within Psyr_4397 at 5259.182 kb on - strand, within Psyr_4397 at 5259.182 kb on - strand, within Psyr_4397 at 5259.182 kb on - strand, within Psyr_4397 at 5259.182 kb on - strand at 5259.182 kb on - strand, within Psyr_4397 at 5259.182 kb on - strand, within Psyr_4397 at 5259.182 kb on - strand, within Psyr_4397 at 5259.182 kb on - strand, within Psyr_4397 at 5259.182 kb on - strand, within Psyr_4397 at 5259.182 kb on - strand, within Psyr_4397 at 5259.182 kb on - strand, within Psyr_4397 at 5259.182 kb on - strand, within Psyr_4397 at 5259.182 kb on - strand, within Psyr_4397 at 5259.182 kb on - strand, within Psyr_4397 at 5259.182 kb on - strand, within Psyr_4397 at 5259.182 kb on - strand, within Psyr_4397 at 5259.182 kb on - strand, within Psyr_4397 at 5259.182 kb on - strand, within Psyr_4397 at 5259.183 kb on + strand, within Psyr_4397 at 5259.183 kb on + strand, within Psyr_4397 at 5259.183 kb on + strand, within Psyr_4397 at 5259.183 kb on + strand, within Psyr_4397 at 5259.183 kb on + strand, within Psyr_4397 at 5259.183 kb on + strand, within Psyr_4397 at 5259.183 kb on + strand, within Psyr_4397 at 5259.183 kb on + strand, within Psyr_4397 at 5259.183 kb on + strand, within Psyr_4397 at 5259.183 kb on + strand, within Psyr_4397 at 5259.183 kb on + strand, within Psyr_4397 at 5259.183 kb on + strand, within Psyr_4397 at 5259.184 kb on - strand, within Psyr_4397 at 5259.184 kb on - strand, within Psyr_4397 at 5259.184 kb on - strand, within Psyr_4397 at 5259.184 kb on - strand, within Psyr_4397 at 5259.184 kb on - strand, within Psyr_4397 at 5259.184 kb on - strand, within Psyr_4397 at 5259.184 kb on - strand, within Psyr_4397 at 5259.184 kb on - strand, within Psyr_4397 at 5259.184 kb on - strand, within Psyr_4397 at 5259.184 kb on - strand, within Psyr_4397 at 5259.184 kb on - strand, within Psyr_4397 at 5259.184 kb on - strand, within Psyr_4397 at 5259.184 kb on - strand, within Psyr_4397 at 5259.187 kb on + strand, within Psyr_4397 at 5259.187 kb on + strand, within Psyr_4397 at 5259.187 kb on + strand, within Psyr_4397 at 5259.187 kb on + strand, within Psyr_4397 at 5259.188 kb on - strand, within Psyr_4397 at 5259.188 kb on - strand, within Psyr_4397 at 5259.188 kb on - strand, within Psyr_4397 at 5259.188 kb on - strand, within Psyr_4397 at 5259.188 kb on - strand, within Psyr_4397 at 5259.188 kb on - strand, within Psyr_4397 at 5259.188 kb on - strand, within Psyr_4397 at 5259.188 kb on - strand, within Psyr_4397 at 5259.188 kb on - strand, within Psyr_4397 at 5259.205 kb on + strand, within Psyr_4397 at 5259.205 kb on + strand, within Psyr_4397 at 5259.247 kb on + strand at 5259.286 kb on + strand at 5259.286 kb on + strand at 5259.286 kb on + strand at 5259.287 kb on - strand at 5259.287 kb on - strand at 5259.287 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 5,257,400 + -1.2 5,257,400 + -1.6 5,257,400 + -1.1 5,257,401 - +4.3 5,257,401 - -0.1 5,257,401 - -0.5 5,257,401 - -2.0 5,257,401 - +1.2 5,257,401 - -1.3 5,257,401 - -0.4 5,257,401 - +0.4 5,257,403 + +0.2 5,257,403 + +4.7 5,257,404 - -1.3 5,257,404 - +5.0 5,257,404 - -0.9 5,257,404 - +3.2 5,257,535 - Psyr_4395 0.24 -0.9 5,257,535 - Psyr_4395 0.24 +3.0 5,257,535 - Psyr_4395 0.24 -1.5 5,257,705 - Psyr_4395 0.43 +2.8 5,257,705 - Psyr_4395 0.43 +0.4 5,257,776 - Psyr_4395 0.51 -2.0 5,258,091 + Psyr_4395 0.86 +2.2 5,258,091 + Psyr_4395 0.86 +3.0 5,258,091 + Psyr_4395 0.86 -0.9 5,258,091 + Psyr_4395 0.86 +3.0 5,258,092 - Psyr_4395 0.87 +3.0 5,258,092 - Psyr_4395 0.87 -0.5 5,258,092 - Psyr_4395 0.87 +2.2 5,258,181 - -1.6 5,258,181 - -0.9 5,258,181 - +2.2 5,258,188 + +4.0 5,258,188 + +0.2 5,258,189 - -0.9 5,258,189 - +4.1 5,258,189 - +0.8 5,258,219 + +0.3 5,258,219 + +3.5 5,258,220 - +0.2 5,258,220 - +3.4 5,258,220 - -1.5 5,258,220 - +3.5 5,258,262 + -0.2 5,258,262 + -2.0 5,258,262 + +0.3 5,258,263 - -0.2 5,258,265 + +3.0 5,258,265 + -2.5 5,258,265 + -1.3 5,258,265 + +0.9 5,258,265 + -0.3 5,258,266 - -0.7 5,258,496 + Psyr_4396 0.34 +0.1 5,258,497 - Psyr_4396 0.34 -0.6 5,258,525 + Psyr_4396 0.38 +4.2 5,258,687 + Psyr_4396 0.60 -0.0 5,258,795 + Psyr_4396 0.75 +3.9 5,258,795 + Psyr_4396 0.75 +2.2 5,258,796 - Psyr_4396 0.75 -0.8 5,258,796 - Psyr_4396 0.75 +1.0 5,258,971 - -0.6 5,258,971 - +0.6 5,258,990 + -1.3 5,258,990 + -0.9 5,258,990 + +2.2 5,258,991 - -2.0 5,258,991 - +3.5 5,258,991 - -2.1 5,258,991 - -2.4 5,258,991 - +2.2 5,259,005 + Psyr_4397 0.10 -3.7 5,259,005 + Psyr_4397 0.10 +1.7 5,259,005 + Psyr_4397 0.10 +2.8 5,259,005 + Psyr_4397 0.10 +3.0 5,259,006 - Psyr_4397 0.11 -1.8 5,259,006 - Psyr_4397 0.11 +3.8 5,259,006 - Psyr_4397 0.11 -2.7 5,259,181 + Psyr_4397 0.75 +3.2 5,259,181 + Psyr_4397 0.75 -1.5 5,259,181 + Psyr_4397 0.75 -0.8 5,259,181 + Psyr_4397 0.75 +1.8 5,259,181 + Psyr_4397 0.75 +1.2 5,259,181 + Psyr_4397 0.75 +0.3 5,259,181 + Psyr_4397 0.75 +1.8 5,259,181 + Psyr_4397 0.75 -0.8 5,259,181 + Psyr_4397 0.75 -2.7 5,259,181 + Psyr_4397 0.75 -1.7 5,259,181 + Psyr_4397 0.75 -2.8 5,259,181 + Psyr_4397 0.75 +2.2 5,259,181 + Psyr_4397 0.75 -1.0 5,259,181 + Psyr_4397 0.75 +3.5 5,259,182 - Psyr_4397 0.75 +1.4 5,259,182 - Psyr_4397 0.75 +4.1 5,259,182 - Psyr_4397 0.75 -1.7 5,259,182 - +2.2 5,259,182 - Psyr_4397 0.75 -1.0 5,259,182 - Psyr_4397 0.75 +0.2 5,259,182 - Psyr_4397 0.75 -1.4 5,259,182 - Psyr_4397 0.75 -2.3 5,259,182 - Psyr_4397 0.75 +0.2 5,259,182 - Psyr_4397 0.75 +4.1 5,259,182 - Psyr_4397 0.75 -2.6 5,259,182 - Psyr_4397 0.75 -0.6 5,259,182 - Psyr_4397 0.75 +0.6 5,259,182 - Psyr_4397 0.75 -0.8 5,259,182 - Psyr_4397 0.75 +1.1 5,259,182 - Psyr_4397 0.75 -1.5 5,259,182 - Psyr_4397 0.75 +1.2 5,259,182 - Psyr_4397 0.75 -1.4 5,259,183 + Psyr_4397 0.75 +1.7 5,259,183 + Psyr_4397 0.75 -0.2 5,259,183 + Psyr_4397 0.75 -1.7 5,259,183 + Psyr_4397 0.75 -1.9 5,259,183 + Psyr_4397 0.75 +3.0 5,259,183 + Psyr_4397 0.75 -0.8 5,259,183 + Psyr_4397 0.75 +2.5 5,259,183 + Psyr_4397 0.75 +1.0 5,259,183 + Psyr_4397 0.75 -0.9 5,259,183 + Psyr_4397 0.75 -0.7 5,259,183 + Psyr_4397 0.75 -0.8 5,259,183 + Psyr_4397 0.75 -0.1 5,259,184 - Psyr_4397 0.76 -0.7 5,259,184 - Psyr_4397 0.76 +0.4 5,259,184 - Psyr_4397 0.76 +0.4 5,259,184 - Psyr_4397 0.76 -3.5 5,259,184 - Psyr_4397 0.76 -0.4 5,259,184 - Psyr_4397 0.76 -1.9 5,259,184 - Psyr_4397 0.76 -2.8 5,259,184 - Psyr_4397 0.76 -0.3 5,259,184 - Psyr_4397 0.76 +0.2 5,259,184 - Psyr_4397 0.76 -1.6 5,259,184 - Psyr_4397 0.76 -1.1 5,259,184 - Psyr_4397 0.76 +1.8 5,259,184 - Psyr_4397 0.76 +2.7 5,259,187 + Psyr_4397 0.77 +4.4 5,259,187 + Psyr_4397 0.77 -0.6 5,259,187 + Psyr_4397 0.77 -1.7 5,259,187 + Psyr_4397 0.77 -1.6 5,259,188 - Psyr_4397 0.77 -2.0 5,259,188 - Psyr_4397 0.77 -0.4 5,259,188 - Psyr_4397 0.77 +1.8 5,259,188 - Psyr_4397 0.77 +0.6 5,259,188 - Psyr_4397 0.77 +0.2 5,259,188 - Psyr_4397 0.77 +0.0 5,259,188 - Psyr_4397 0.77 +3.5 5,259,188 - Psyr_4397 0.77 +0.2 5,259,188 - Psyr_4397 0.77 +1.2 5,259,205 + Psyr_4397 0.84 -0.2 5,259,205 + Psyr_4397 0.84 +3.8 5,259,247 + -2.9 5,259,286 + +2.5 5,259,286 + +0.0 5,259,286 + -3.5 5,259,287 - -0.5 5,259,287 - +0.4 5,259,287 - +4.5
Or see this region's nucleotide sequence