Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4377 and Psyr_4378 are separated by 264 nucleotides Psyr_4378 and Psyr_4379 overlap by 1 nucleotides Psyr_4379 and Psyr_4380 are separated by 188 nucleotides
Psyr_4377: Psyr_4377 - response regulator receiver modulated diguanylate cyclase, at 5,232,552 to 5,234,207
_4377
Psyr_4378: Psyr_4378 - conserved hypothetical protein, at 5,234,472 to 5,235,551
_4378
Psyr_4379: Psyr_4379 - NUDIX hydrolase, at 5,235,551 to 5,236,096
_4379
Psyr_4380: Psyr_4380 - translation initiation factor 1 (eIF-1/SUI1), at 5,236,285 to 5,236,656
_4380
Position (kb) 5234
5235
5236 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 5233.827 kb on + strand, within Psyr_4377 at 5233.827 kb on + strand, within Psyr_4377 at 5233.827 kb on + strand, within Psyr_4377 at 5233.827 kb on + strand, within Psyr_4377 at 5233.827 kb on + strand, within Psyr_4377 at 5233.827 kb on + strand, within Psyr_4377 at 5233.827 kb on + strand, within Psyr_4377 at 5233.827 kb on + strand, within Psyr_4377 at 5233.827 kb on + strand, within Psyr_4377 at 5233.827 kb on + strand, within Psyr_4377 at 5233.827 kb on + strand, within Psyr_4377 at 5233.827 kb on + strand, within Psyr_4377 at 5233.827 kb on + strand, within Psyr_4377 at 5233.827 kb on + strand, within Psyr_4377 at 5233.828 kb on - strand, within Psyr_4377 at 5233.828 kb on - strand, within Psyr_4377 at 5233.828 kb on - strand, within Psyr_4377 at 5233.828 kb on - strand, within Psyr_4377 at 5233.828 kb on - strand, within Psyr_4377 at 5233.828 kb on - strand, within Psyr_4377 at 5233.828 kb on - strand, within Psyr_4377 at 5233.828 kb on - strand, within Psyr_4377 at 5233.828 kb on - strand, within Psyr_4377 at 5233.828 kb on - strand, within Psyr_4377 at 5233.828 kb on - strand, within Psyr_4377 at 5233.828 kb on - strand, within Psyr_4377 at 5233.828 kb on - strand, within Psyr_4377 at 5233.828 kb on - strand, within Psyr_4377 at 5233.828 kb on - strand, within Psyr_4377 at 5233.829 kb on + strand, within Psyr_4377 at 5233.829 kb on + strand, within Psyr_4377 at 5233.829 kb on + strand, within Psyr_4377 at 5233.829 kb on + strand, within Psyr_4377 at 5233.829 kb on + strand, within Psyr_4377 at 5233.829 kb on + strand, within Psyr_4377 at 5233.829 kb on + strand, within Psyr_4377 at 5233.829 kb on + strand, within Psyr_4377 at 5233.830 kb on - strand, within Psyr_4377 at 5233.830 kb on - strand, within Psyr_4377 at 5233.830 kb on - strand, within Psyr_4377 at 5233.830 kb on - strand, within Psyr_4377 at 5233.830 kb on - strand, within Psyr_4377 at 5233.830 kb on - strand, within Psyr_4377 at 5233.830 kb on - strand, within Psyr_4377 at 5233.830 kb on - strand, within Psyr_4377 at 5233.830 kb on - strand, within Psyr_4377 at 5233.831 kb on + strand, within Psyr_4377 at 5233.831 kb on + strand, within Psyr_4377 at 5233.831 kb on + strand, within Psyr_4377 at 5233.831 kb on + strand, within Psyr_4377 at 5233.831 kb on + strand, within Psyr_4377 at 5233.831 kb on + strand, within Psyr_4377 at 5233.831 kb on + strand, within Psyr_4377 at 5233.831 kb on + strand, within Psyr_4377 at 5233.832 kb on - strand, within Psyr_4377 at 5233.832 kb on - strand, within Psyr_4377 at 5233.832 kb on - strand, within Psyr_4377 at 5233.832 kb on - strand, within Psyr_4377 at 5233.832 kb on - strand, within Psyr_4377 at 5233.832 kb on - strand, within Psyr_4377 at 5233.832 kb on - strand, within Psyr_4377 at 5233.832 kb on - strand, within Psyr_4377 at 5233.832 kb on - strand, within Psyr_4377 at 5234.216 kb on + strand at 5234.217 kb on - strand at 5234.217 kb on - strand at 5234.257 kb on + strand at 5234.258 kb on - strand at 5234.269 kb on + strand at 5234.269 kb on + strand at 5234.270 kb on - strand at 5234.287 kb on + strand at 5234.288 kb on - strand at 5234.288 kb on - strand at 5234.296 kb on + strand at 5234.297 kb on - strand at 5234.360 kb on + strand at 5234.361 kb on - strand at 5234.380 kb on + strand at 5234.380 kb on + strand at 5234.388 kb on + strand at 5234.388 kb on + strand at 5234.388 kb on + strand at 5234.388 kb on + strand at 5234.389 kb on - strand at 5234.389 kb on - strand at 5234.389 kb on - strand at 5234.389 kb on - strand at 5234.389 kb on - strand at 5234.389 kb on - strand at 5234.414 kb on + strand at 5234.414 kb on + strand at 5234.414 kb on + strand at 5234.414 kb on + strand at 5234.468 kb on + strand at 5234.468 kb on + strand at 5234.469 kb on - strand at 5234.565 kb on + strand at 5234.565 kb on + strand at 5234.565 kb on + strand at 5234.565 kb on + strand at 5234.565 kb on + strand at 5234.565 kb on + strand at 5234.565 kb on + strand at 5234.565 kb on + strand at 5234.565 kb on + strand at 5234.566 kb on - strand at 5234.566 kb on - strand at 5234.566 kb on - strand at 5234.566 kb on - strand at 5234.566 kb on - strand at 5234.566 kb on - strand at 5234.566 kb on - strand at 5234.568 kb on - strand at 5234.598 kb on + strand, within Psyr_4378 at 5234.598 kb on + strand, within Psyr_4378 at 5234.598 kb on + strand, within Psyr_4378 at 5234.598 kb on + strand, within Psyr_4378 at 5234.598 kb on + strand, within Psyr_4378 at 5234.598 kb on + strand, within Psyr_4378 at 5234.598 kb on + strand, within Psyr_4378 at 5234.598 kb on + strand, within Psyr_4378 at 5234.598 kb on + strand, within Psyr_4378 at 5234.599 kb on - strand, within Psyr_4378 at 5234.599 kb on - strand, within Psyr_4378 at 5234.599 kb on - strand, within Psyr_4378 at 5234.775 kb on + strand, within Psyr_4378 at 5234.835 kb on + strand, within Psyr_4378 at 5235.070 kb on - strand, within Psyr_4378 at 5235.279 kb on + strand, within Psyr_4378 at 5235.279 kb on + strand, within Psyr_4378 at 5235.279 kb on + strand, within Psyr_4378 at 5235.280 kb on - strand, within Psyr_4378 at 5235.280 kb on - strand, within Psyr_4378 at 5235.349 kb on + strand, within Psyr_4378 at 5235.349 kb on + strand, within Psyr_4378 at 5235.349 kb on + strand, within Psyr_4378 at 5235.349 kb on + strand, within Psyr_4378 at 5235.350 kb on - strand, within Psyr_4378 at 5235.350 kb on - strand, within Psyr_4378 at 5235.350 kb on - strand, within Psyr_4378 at 5235.350 kb on - strand, within Psyr_4378 at 5235.492 kb on + strand at 5235.492 kb on + strand at 5235.492 kb on + strand at 5235.492 kb on + strand at 5235.492 kb on + strand at 5235.492 kb on + strand at 5235.492 kb on + strand at 5235.493 kb on - strand at 5235.493 kb on - strand at 5235.493 kb on - strand at 5235.493 kb on - strand at 5235.493 kb on - strand at 5235.493 kb on - strand at 5235.493 kb on - strand at 5235.493 kb on - strand at 5235.493 kb on - strand at 5235.493 kb on - strand at 5235.493 kb on - strand at 5235.493 kb on - strand at 5235.493 kb on - strand at 5235.493 kb on - strand at 5235.493 kb on - strand at 5235.493 kb on - strand at 5235.558 kb on + strand at 5235.559 kb on - strand at 5235.692 kb on + strand, within Psyr_4379 at 5235.692 kb on + strand, within Psyr_4379 at 5235.692 kb on + strand, within Psyr_4379 at 5235.692 kb on + strand, within Psyr_4379 at 5235.692 kb on + strand, within Psyr_4379 at 5235.692 kb on + strand, within Psyr_4379 at 5235.692 kb on + strand, within Psyr_4379 at 5235.692 kb on + strand, within Psyr_4379 at 5235.692 kb on + strand, within Psyr_4379 at 5235.692 kb on + strand, within Psyr_4379 at 5235.692 kb on + strand, within Psyr_4379 at 5235.692 kb on + strand, within Psyr_4379 at 5235.692 kb on + strand, within Psyr_4379 at 5235.692 kb on + strand, within Psyr_4379 at 5235.692 kb on + strand, within Psyr_4379 at 5235.692 kb on + strand, within Psyr_4379 at 5235.692 kb on + strand, within Psyr_4379 at 5235.692 kb on + strand, within Psyr_4379 at 5235.692 kb on + strand, within Psyr_4379 at 5235.692 kb on + strand, within Psyr_4379 at 5235.692 kb on + strand, within Psyr_4379 at 5235.692 kb on + strand, within Psyr_4379 at 5235.693 kb on - strand, within Psyr_4379 at 5235.693 kb on - strand, within Psyr_4379 at 5235.693 kb on - strand, within Psyr_4379 at 5235.693 kb on - strand, within Psyr_4379 at 5235.693 kb on - strand, within Psyr_4379 at 5235.693 kb on - strand, within Psyr_4379 at 5235.693 kb on - strand, within Psyr_4379 at 5235.693 kb on - strand, within Psyr_4379 at 5235.693 kb on - strand, within Psyr_4379 at 5235.693 kb on - strand, within Psyr_4379 at 5235.693 kb on - strand, within Psyr_4379 at 5235.693 kb on - strand, within Psyr_4379 at 5235.693 kb on - strand, within Psyr_4379 at 5235.693 kb on - strand, within Psyr_4379 at 5235.693 kb on - strand, within Psyr_4379 at 5235.693 kb on - strand, within Psyr_4379 at 5235.693 kb on - strand, within Psyr_4379 at 5235.693 kb on - strand, within Psyr_4379 at 5235.693 kb on - strand, within Psyr_4379 at 5235.694 kb on + strand, within Psyr_4379 at 5235.694 kb on + strand, within Psyr_4379 at 5235.695 kb on - strand, within Psyr_4379 at 5235.758 kb on + strand, within Psyr_4379 at 5235.758 kb on + strand, within Psyr_4379 at 5235.759 kb on - strand, within Psyr_4379 at 5235.759 kb on - strand, within Psyr_4379 at 5235.777 kb on + strand, within Psyr_4379 at 5235.812 kb on + strand, within Psyr_4379 at 5235.812 kb on + strand, within Psyr_4379 at 5235.813 kb on - strand, within Psyr_4379 at 5235.940 kb on - strand, within Psyr_4379 at 5236.027 kb on + strand, within Psyr_4379 at 5236.027 kb on + strand, within Psyr_4379 at 5236.027 kb on + strand, within Psyr_4379 at 5236.028 kb on - strand, within Psyr_4379 at 5236.028 kb on - strand, within Psyr_4379 at 5236.028 kb on - strand, within Psyr_4379 at 5236.068 kb on - strand at 5236.072 kb on + strand at 5236.073 kb on - strand at 5236.080 kb on + strand at 5236.081 kb on - strand at 5236.241 kb on + strand at 5236.328 kb on + strand, within Psyr_4380 at 5236.328 kb on + strand, within Psyr_4380 at 5236.329 kb on - strand, within Psyr_4380 at 5236.434 kb on - strand, within Psyr_4380 at 5236.434 kb on - strand, within Psyr_4380 at 5236.434 kb on - strand, within Psyr_4380
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 5,233,827 + Psyr_4377 0.77 -1.8 5,233,827 + Psyr_4377 0.77 -2.2 5,233,827 + Psyr_4377 0.77 +0.5 5,233,827 + Psyr_4377 0.77 -1.5 5,233,827 + Psyr_4377 0.77 -2.7 5,233,827 + Psyr_4377 0.77 +2.8 5,233,827 + Psyr_4377 0.77 +0.5 5,233,827 + Psyr_4377 0.77 -0.5 5,233,827 + Psyr_4377 0.77 +3.8 5,233,827 + Psyr_4377 0.77 +1.2 5,233,827 + Psyr_4377 0.77 +0.5 5,233,827 + Psyr_4377 0.77 -1.3 5,233,827 + Psyr_4377 0.77 +2.5 5,233,827 + Psyr_4377 0.77 -0.8 5,233,828 - Psyr_4377 0.77 -0.4 5,233,828 - Psyr_4377 0.77 -0.4 5,233,828 - Psyr_4377 0.77 -0.4 5,233,828 - Psyr_4377 0.77 -2.5 5,233,828 - Psyr_4377 0.77 -1.1 5,233,828 - Psyr_4377 0.77 -1.5 5,233,828 - Psyr_4377 0.77 -2.6 5,233,828 - Psyr_4377 0.77 +4.0 5,233,828 - Psyr_4377 0.77 +2.2 5,233,828 - Psyr_4377 0.77 +0.0 5,233,828 - Psyr_4377 0.77 +0.0 5,233,828 - Psyr_4377 0.77 +1.2 5,233,828 - Psyr_4377 0.77 +1.8 5,233,828 - Psyr_4377 0.77 +3.7 5,233,828 - Psyr_4377 0.77 +1.4 5,233,829 + Psyr_4377 0.77 +2.5 5,233,829 + Psyr_4377 0.77 -2.0 5,233,829 + Psyr_4377 0.77 -1.4 5,233,829 + Psyr_4377 0.77 +1.5 5,233,829 + Psyr_4377 0.77 +0.5 5,233,829 + Psyr_4377 0.77 +0.3 5,233,829 + Psyr_4377 0.77 +3.9 5,233,829 + Psyr_4377 0.77 -1.4 5,233,830 - Psyr_4377 0.77 -2.0 5,233,830 - Psyr_4377 0.77 +3.2 5,233,830 - Psyr_4377 0.77 +3.9 5,233,830 - Psyr_4377 0.77 -1.2 5,233,830 - Psyr_4377 0.77 +2.8 5,233,830 - Psyr_4377 0.77 -1.9 5,233,830 - Psyr_4377 0.77 -1.1 5,233,830 - Psyr_4377 0.77 +4.4 5,233,830 - Psyr_4377 0.77 +3.0 5,233,831 + Psyr_4377 0.77 -1.1 5,233,831 + Psyr_4377 0.77 +0.6 5,233,831 + Psyr_4377 0.77 -3.8 5,233,831 + Psyr_4377 0.77 +0.7 5,233,831 + Psyr_4377 0.77 -0.4 5,233,831 + Psyr_4377 0.77 +3.2 5,233,831 + Psyr_4377 0.77 +0.5 5,233,831 + Psyr_4377 0.77 +2.2 5,233,832 - Psyr_4377 0.77 +1.8 5,233,832 - Psyr_4377 0.77 -1.8 5,233,832 - Psyr_4377 0.77 -1.0 5,233,832 - Psyr_4377 0.77 +2.2 5,233,832 - Psyr_4377 0.77 +2.2 5,233,832 - Psyr_4377 0.77 -0.4 5,233,832 - Psyr_4377 0.77 +3.9 5,233,832 - Psyr_4377 0.77 +0.4 5,233,832 - Psyr_4377 0.77 -1.0 5,234,216 + +2.0 5,234,217 - -1.6 5,234,217 - +0.0 5,234,257 + +0.8 5,234,258 - +0.9 5,234,269 + -0.1 5,234,269 + +1.8 5,234,270 - -2.0 5,234,287 + -1.5 5,234,288 - -0.3 5,234,288 - +0.2 5,234,296 + +1.2 5,234,297 - -2.2 5,234,360 + -0.4 5,234,361 - -2.6 5,234,380 + -0.9 5,234,380 + -3.9 5,234,388 + +1.0 5,234,388 + -0.2 5,234,388 + +1.0 5,234,388 + -0.6 5,234,389 - -0.2 5,234,389 - +2.5 5,234,389 - -2.2 5,234,389 - -0.7 5,234,389 - -1.3 5,234,389 - -0.6 5,234,414 + +0.3 5,234,414 + +0.2 5,234,414 + -0.9 5,234,414 + -1.4 5,234,468 + -0.1 5,234,468 + +0.4 5,234,469 - -0.3 5,234,565 + -0.3 5,234,565 + -2.6 5,234,565 + +0.1 5,234,565 + -1.6 5,234,565 + -1.8 5,234,565 + -2.0 5,234,565 + -2.5 5,234,565 + -1.8 5,234,565 + +3.7 5,234,566 - +2.8 5,234,566 - +2.8 5,234,566 - -2.4 5,234,566 - -0.5 5,234,566 - +0.2 5,234,566 - -2.2 5,234,566 - +2.5 5,234,568 - -2.4 5,234,598 + Psyr_4378 0.12 -0.4 5,234,598 + Psyr_4378 0.12 +4.4 5,234,598 + Psyr_4378 0.12 +1.8 5,234,598 + Psyr_4378 0.12 +3.0 5,234,598 + Psyr_4378 0.12 +0.2 5,234,598 + Psyr_4378 0.12 +2.5 5,234,598 + Psyr_4378 0.12 +3.7 5,234,598 + Psyr_4378 0.12 +0.0 5,234,598 + Psyr_4378 0.12 -2.0 5,234,599 - Psyr_4378 0.12 +1.8 5,234,599 - Psyr_4378 0.12 -0.9 5,234,599 - Psyr_4378 0.12 +0.9 5,234,775 + Psyr_4378 0.28 +2.3 5,234,835 + Psyr_4378 0.34 +3.9 5,235,070 - Psyr_4378 0.55 +5.0 5,235,279 + Psyr_4378 0.75 +0.5 5,235,279 + Psyr_4378 0.75 +1.2 5,235,279 + Psyr_4378 0.75 +0.2 5,235,280 - Psyr_4378 0.75 -0.9 5,235,280 - Psyr_4378 0.75 +2.2 5,235,349 + Psyr_4378 0.81 +0.2 5,235,349 + Psyr_4378 0.81 -0.9 5,235,349 + Psyr_4378 0.81 +0.0 5,235,349 + Psyr_4378 0.81 -0.5 5,235,350 - Psyr_4378 0.81 +2.2 5,235,350 - Psyr_4378 0.81 -0.1 5,235,350 - Psyr_4378 0.81 -1.2 5,235,350 - Psyr_4378 0.81 -0.0 5,235,492 + +4.4 5,235,492 + +1.2 5,235,492 + -0.8 5,235,492 + +0.8 5,235,492 + -0.2 5,235,492 + +2.2 5,235,492 + +2.2 5,235,493 - +2.2 5,235,493 - -0.9 5,235,493 - +0.1 5,235,493 - -0.8 5,235,493 - -1.8 5,235,493 - -0.2 5,235,493 - +2.2 5,235,493 - +0.2 5,235,493 - +0.1 5,235,493 - +0.0 5,235,493 - -0.7 5,235,493 - -0.4 5,235,493 - +4.1 5,235,493 - +0.6 5,235,493 - +3.5 5,235,493 - -0.5 5,235,558 + -2.0 5,235,559 - +3.2 5,235,692 + Psyr_4379 0.26 -1.8 5,235,692 + Psyr_4379 0.26 +4.2 5,235,692 + Psyr_4379 0.26 +2.2 5,235,692 + Psyr_4379 0.26 -1.9 5,235,692 + Psyr_4379 0.26 +0.7 5,235,692 + Psyr_4379 0.26 -4.4 5,235,692 + Psyr_4379 0.26 -1.1 5,235,692 + Psyr_4379 0.26 +3.4 5,235,692 + Psyr_4379 0.26 -4.4 5,235,692 + Psyr_4379 0.26 +1.2 5,235,692 + Psyr_4379 0.26 +0.2 5,235,692 + Psyr_4379 0.26 +3.0 5,235,692 + Psyr_4379 0.26 +0.2 5,235,692 + Psyr_4379 0.26 +3.2 5,235,692 + Psyr_4379 0.26 +3.7 5,235,692 + Psyr_4379 0.26 -0.7 5,235,692 + Psyr_4379 0.26 +0.6 5,235,692 + Psyr_4379 0.26 -1.1 5,235,692 + Psyr_4379 0.26 -0.3 5,235,692 + Psyr_4379 0.26 -1.4 5,235,692 + Psyr_4379 0.26 -1.1 5,235,692 + Psyr_4379 0.26 +0.9 5,235,693 - Psyr_4379 0.26 +4.1 5,235,693 - Psyr_4379 0.26 +1.2 5,235,693 - Psyr_4379 0.26 -3.3 5,235,693 - Psyr_4379 0.26 +2.5 5,235,693 - Psyr_4379 0.26 +0.8 5,235,693 - Psyr_4379 0.26 -2.3 5,235,693 - Psyr_4379 0.26 -1.4 5,235,693 - Psyr_4379 0.26 +3.2 5,235,693 - Psyr_4379 0.26 -2.1 5,235,693 - Psyr_4379 0.26 +1.8 5,235,693 - Psyr_4379 0.26 +0.3 5,235,693 - Psyr_4379 0.26 -2.6 5,235,693 - Psyr_4379 0.26 +2.5 5,235,693 - Psyr_4379 0.26 -0.8 5,235,693 - Psyr_4379 0.26 -0.6 5,235,693 - Psyr_4379 0.26 -0.6 5,235,693 - Psyr_4379 0.26 +2.8 5,235,693 - Psyr_4379 0.26 -1.3 5,235,693 - Psyr_4379 0.26 +2.5 5,235,694 + Psyr_4379 0.26 -1.6 5,235,694 + Psyr_4379 0.26 +3.9 5,235,695 - Psyr_4379 0.26 +1.2 5,235,758 + Psyr_4379 0.38 -1.6 5,235,758 + Psyr_4379 0.38 -1.8 5,235,759 - Psyr_4379 0.38 +3.0 5,235,759 - Psyr_4379 0.38 +0.9 5,235,777 + Psyr_4379 0.41 -2.2 5,235,812 + Psyr_4379 0.48 -0.3 5,235,812 + Psyr_4379 0.48 +2.2 5,235,813 - Psyr_4379 0.48 +3.2 5,235,940 - Psyr_4379 0.71 +1.2 5,236,027 + Psyr_4379 0.87 -0.3 5,236,027 + Psyr_4379 0.87 +1.2 5,236,027 + Psyr_4379 0.87 +1.2 5,236,028 - Psyr_4379 0.87 -0.7 5,236,028 - Psyr_4379 0.87 -1.2 5,236,028 - Psyr_4379 0.87 +0.9 5,236,068 - -0.5 5,236,072 + -0.8 5,236,073 - -1.5 5,236,080 + -0.8 5,236,081 - +1.3 5,236,241 + +1.3 5,236,328 + Psyr_4380 0.12 -0.7 5,236,328 + Psyr_4380 0.12 -0.4 5,236,329 - Psyr_4380 0.12 -0.1 5,236,434 - Psyr_4380 0.40 -2.3 5,236,434 - Psyr_4380 0.40 -0.5 5,236,434 - Psyr_4380 0.40 +0.4
Or see this region's nucleotide sequence