Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4335 and Psyr_4336 are separated by 13 nucleotides Psyr_4336 and Psyr_4337 are separated by 79 nucleotides Psyr_4337 and Psyr_4338 are separated by 52 nucleotides
Psyr_4335: Psyr_4335 - transcriptional regulator, AsnC family, at 5,187,086 to 5,187,577
_4335
Psyr_4336: Psyr_4336 - ribosomal large subunit pseudouridine synthase E, at 5,187,591 to 5,188,223
_4336
Psyr_4337: Psyr_4337 - AMP nucleosidase, at 5,188,303 to 5,189,766
_4337
Psyr_4338: Psyr_4338 - Acyl-CoA dehydrogenase, C-terminal:Acyl-CoA dehydrogenase, central region, at 5,189,819 to 5,191,468
_4338
Position (kb) 5188
5189
5190 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 5187.303 kb on - strand, within Psyr_4335 at 5187.420 kb on - strand, within Psyr_4335 at 5187.922 kb on + strand, within Psyr_4336 at 5187.923 kb on - strand, within Psyr_4336 at 5187.923 kb on - strand, within Psyr_4336 at 5188.030 kb on + strand, within Psyr_4336 at 5188.030 kb on + strand, within Psyr_4336 at 5188.031 kb on - strand, within Psyr_4336 at 5188.031 kb on - strand, within Psyr_4336 at 5188.279 kb on + strand at 5188.279 kb on + strand at 5188.280 kb on - strand at 5188.280 kb on - strand at 5188.280 kb on - strand at 5188.287 kb on + strand at 5188.288 kb on - strand at 5188.288 kb on - strand at 5188.288 kb on - strand at 5188.424 kb on + strand at 5188.424 kb on + strand at 5188.425 kb on - strand at 5188.425 kb on - strand at 5188.614 kb on + strand, within Psyr_4337 at 5188.615 kb on - strand, within Psyr_4337 at 5188.625 kb on + strand, within Psyr_4337 at 5188.625 kb on + strand, within Psyr_4337 at 5188.626 kb on - strand, within Psyr_4337 at 5188.626 kb on - strand, within Psyr_4337 at 5188.626 kb on - strand, within Psyr_4337 at 5188.787 kb on + strand, within Psyr_4337 at 5188.787 kb on + strand, within Psyr_4337 at 5188.787 kb on + strand, within Psyr_4337 at 5188.787 kb on + strand, within Psyr_4337 at 5188.787 kb on + strand, within Psyr_4337 at 5188.788 kb on - strand, within Psyr_4337 at 5188.788 kb on - strand, within Psyr_4337 at 5188.788 kb on - strand, within Psyr_4337 at 5188.788 kb on - strand, within Psyr_4337 at 5188.788 kb on - strand, within Psyr_4337 at 5188.788 kb on - strand, within Psyr_4337 at 5188.788 kb on - strand, within Psyr_4337 at 5188.788 kb on - strand, within Psyr_4337 at 5188.876 kb on + strand, within Psyr_4337 at 5188.876 kb on + strand, within Psyr_4337 at 5189.051 kb on - strand, within Psyr_4337 at 5189.135 kb on + strand, within Psyr_4337 at 5189.136 kb on - strand, within Psyr_4337 at 5189.136 kb on - strand, within Psyr_4337 at 5189.136 kb on - strand, within Psyr_4337 at 5189.144 kb on + strand, within Psyr_4337 at 5189.144 kb on + strand, within Psyr_4337 at 5189.144 kb on + strand, within Psyr_4337 at 5189.145 kb on - strand, within Psyr_4337 at 5189.145 kb on - strand, within Psyr_4337 at 5189.145 kb on - strand, within Psyr_4337 at 5189.145 kb on - strand, within Psyr_4337 at 5189.145 kb on - strand, within Psyr_4337 at 5189.170 kb on + strand, within Psyr_4337 at 5189.170 kb on + strand, within Psyr_4337 at 5189.170 kb on + strand, within Psyr_4337 at 5189.171 kb on - strand, within Psyr_4337 at 5189.171 kb on - strand, within Psyr_4337 at 5189.171 kb on - strand, within Psyr_4337 at 5189.171 kb on - strand, within Psyr_4337 at 5189.171 kb on - strand, within Psyr_4337 at 5189.171 kb on - strand, within Psyr_4337 at 5189.195 kb on + strand, within Psyr_4337 at 5189.195 kb on + strand, within Psyr_4337 at 5189.195 kb on + strand, within Psyr_4337 at 5189.195 kb on + strand, within Psyr_4337 at 5189.195 kb on + strand, within Psyr_4337 at 5189.196 kb on - strand, within Psyr_4337 at 5189.196 kb on - strand, within Psyr_4337 at 5189.196 kb on - strand, within Psyr_4337 at 5189.196 kb on - strand, within Psyr_4337 at 5189.196 kb on - strand, within Psyr_4337 at 5189.279 kb on + strand, within Psyr_4337 at 5189.279 kb on + strand, within Psyr_4337 at 5189.279 kb on + strand, within Psyr_4337 at 5189.279 kb on + strand, within Psyr_4337 at 5189.279 kb on + strand, within Psyr_4337 at 5189.280 kb on - strand, within Psyr_4337 at 5189.396 kb on + strand, within Psyr_4337 at 5189.396 kb on + strand, within Psyr_4337 at 5189.396 kb on + strand, within Psyr_4337 at 5189.397 kb on - strand, within Psyr_4337 at 5189.397 kb on - strand, within Psyr_4337 at 5189.397 kb on - strand, within Psyr_4337 at 5189.484 kb on - strand, within Psyr_4337 at 5189.484 kb on - strand, within Psyr_4337 at 5189.522 kb on + strand, within Psyr_4337 at 5189.523 kb on - strand, within Psyr_4337 at 5189.523 kb on - strand, within Psyr_4337 at 5189.523 kb on - strand, within Psyr_4337 at 5189.523 kb on - strand, within Psyr_4337 at 5189.523 kb on - strand, within Psyr_4337 at 5189.588 kb on + strand, within Psyr_4337 at 5189.588 kb on + strand, within Psyr_4337 at 5189.588 kb on + strand, within Psyr_4337 at 5189.589 kb on - strand, within Psyr_4337 at 5189.589 kb on - strand, within Psyr_4337 at 5189.589 kb on - strand, within Psyr_4337 at 5189.589 kb on - strand, within Psyr_4337 at 5189.660 kb on + strand at 5189.661 kb on - strand at 5189.722 kb on + strand at 5189.722 kb on + strand at 5189.859 kb on - strand at 5190.241 kb on - strand, within Psyr_4338 at 5190.241 kb on - strand, within Psyr_4338 at 5190.306 kb on + strand, within Psyr_4338 at 5190.306 kb on + strand, within Psyr_4338 at 5190.307 kb on - strand, within Psyr_4338 at 5190.307 kb on - strand, within Psyr_4338 at 5190.307 kb on - strand, within Psyr_4338 at 5190.307 kb on - strand, within Psyr_4338 at 5190.696 kb on + strand, within Psyr_4338 at 5190.696 kb on + strand, within Psyr_4338 at 5190.696 kb on + strand, within Psyr_4338 at 5190.697 kb on - strand, within Psyr_4338
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 5,187,303 - Psyr_4335 0.44 +2.7 5,187,420 - Psyr_4335 0.68 -1.9 5,187,922 + Psyr_4336 0.52 +1.7 5,187,923 - Psyr_4336 0.52 +1.2 5,187,923 - Psyr_4336 0.52 +2.7 5,188,030 + Psyr_4336 0.69 -1.1 5,188,030 + Psyr_4336 0.69 -1.2 5,188,031 - Psyr_4336 0.70 +2.2 5,188,031 - Psyr_4336 0.70 -3.0 5,188,279 + +4.7 5,188,279 + +0.2 5,188,280 - -0.1 5,188,280 - +0.9 5,188,280 - +0.5 5,188,287 + +2.2 5,188,288 - -0.3 5,188,288 - +1.9 5,188,288 - +0.2 5,188,424 + +0.6 5,188,424 + +0.1 5,188,425 - +4.0 5,188,425 - +4.4 5,188,614 + Psyr_4337 0.21 -0.7 5,188,615 - Psyr_4337 0.21 -0.9 5,188,625 + Psyr_4337 0.22 -0.5 5,188,625 + Psyr_4337 0.22 +4.5 5,188,626 - Psyr_4337 0.22 +0.2 5,188,626 - Psyr_4337 0.22 +1.3 5,188,626 - Psyr_4337 0.22 +3.6 5,188,787 + Psyr_4337 0.33 -1.7 5,188,787 + Psyr_4337 0.33 -0.5 5,188,787 + Psyr_4337 0.33 +4.2 5,188,787 + Psyr_4337 0.33 -0.6 5,188,787 + Psyr_4337 0.33 +3.9 5,188,788 - Psyr_4337 0.33 -2.3 5,188,788 - Psyr_4337 0.33 +2.7 5,188,788 - Psyr_4337 0.33 +1.4 5,188,788 - Psyr_4337 0.33 +3.7 5,188,788 - Psyr_4337 0.33 -0.1 5,188,788 - Psyr_4337 0.33 +3.3 5,188,788 - Psyr_4337 0.33 -2.4 5,188,788 - Psyr_4337 0.33 +1.7 5,188,876 + Psyr_4337 0.39 -0.2 5,188,876 + Psyr_4337 0.39 +3.7 5,189,051 - Psyr_4337 0.51 -0.6 5,189,135 + Psyr_4337 0.57 -0.3 5,189,136 - Psyr_4337 0.57 +3.5 5,189,136 - Psyr_4337 0.57 -0.1 5,189,136 - Psyr_4337 0.57 +0.2 5,189,144 + Psyr_4337 0.57 -0.6 5,189,144 + Psyr_4337 0.57 +0.7 5,189,144 + Psyr_4337 0.57 +0.2 5,189,145 - Psyr_4337 0.58 +5.2 5,189,145 - Psyr_4337 0.58 -1.1 5,189,145 - Psyr_4337 0.58 -0.8 5,189,145 - Psyr_4337 0.58 -1.4 5,189,145 - Psyr_4337 0.58 -1.7 5,189,170 + Psyr_4337 0.59 +1.6 5,189,170 + Psyr_4337 0.59 -1.4 5,189,170 + Psyr_4337 0.59 -0.5 5,189,171 - Psyr_4337 0.59 +4.6 5,189,171 - Psyr_4337 0.59 -2.8 5,189,171 - Psyr_4337 0.59 -0.6 5,189,171 - Psyr_4337 0.59 +0.4 5,189,171 - Psyr_4337 0.59 +0.6 5,189,171 - Psyr_4337 0.59 -1.9 5,189,195 + Psyr_4337 0.61 -1.4 5,189,195 + Psyr_4337 0.61 -0.5 5,189,195 + Psyr_4337 0.61 +4.1 5,189,195 + Psyr_4337 0.61 -1.1 5,189,195 + Psyr_4337 0.61 +1.2 5,189,196 - Psyr_4337 0.61 -0.6 5,189,196 - Psyr_4337 0.61 +2.7 5,189,196 - Psyr_4337 0.61 +0.4 5,189,196 - Psyr_4337 0.61 +1.1 5,189,196 - Psyr_4337 0.61 -0.6 5,189,279 + Psyr_4337 0.67 -0.6 5,189,279 + Psyr_4337 0.67 +2.0 5,189,279 + Psyr_4337 0.67 -0.4 5,189,279 + Psyr_4337 0.67 +0.0 5,189,279 + Psyr_4337 0.67 +4.3 5,189,280 - Psyr_4337 0.67 +1.4 5,189,396 + Psyr_4337 0.75 -0.0 5,189,396 + Psyr_4337 0.75 -0.7 5,189,396 + Psyr_4337 0.75 -3.0 5,189,397 - Psyr_4337 0.75 +3.0 5,189,397 - Psyr_4337 0.75 +0.1 5,189,397 - Psyr_4337 0.75 -0.9 5,189,484 - Psyr_4337 0.81 +2.5 5,189,484 - Psyr_4337 0.81 +0.9 5,189,522 + Psyr_4337 0.83 +3.3 5,189,523 - Psyr_4337 0.83 +4.2 5,189,523 - Psyr_4337 0.83 -2.3 5,189,523 - Psyr_4337 0.83 -1.5 5,189,523 - Psyr_4337 0.83 -0.6 5,189,523 - Psyr_4337 0.83 -0.1 5,189,588 + Psyr_4337 0.88 +1.2 5,189,588 + Psyr_4337 0.88 +0.4 5,189,588 + Psyr_4337 0.88 +1.2 5,189,589 - Psyr_4337 0.88 -1.9 5,189,589 - Psyr_4337 0.88 +0.1 5,189,589 - Psyr_4337 0.88 +3.2 5,189,589 - Psyr_4337 0.88 +3.1 5,189,660 + -1.1 5,189,661 - -4.0 5,189,722 + -2.0 5,189,722 + -1.1 5,189,859 - -1.0 5,190,241 - Psyr_4338 0.26 +1.0 5,190,241 - Psyr_4338 0.26 +1.9 5,190,306 + Psyr_4338 0.30 +0.2 5,190,306 + Psyr_4338 0.30 +0.2 5,190,307 - Psyr_4338 0.30 +4.3 5,190,307 - Psyr_4338 0.30 -1.5 5,190,307 - Psyr_4338 0.30 -2.3 5,190,307 - Psyr_4338 0.30 +3.2 5,190,696 + Psyr_4338 0.53 +3.6 5,190,696 + Psyr_4338 0.53 +2.7 5,190,696 + Psyr_4338 0.53 -0.9 5,190,697 - Psyr_4338 0.53 -0.8
Or see this region's nucleotide sequence