Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4334 and Psyr_4335 are separated by 381 nucleotides Psyr_4335 and Psyr_4336 are separated by 13 nucleotides Psyr_4336 and Psyr_4337 are separated by 79 nucleotides
Psyr_4334: Psyr_4334 - conserved hypothetical protein, at 5,185,538 to 5,186,704
_4334
Psyr_4335: Psyr_4335 - transcriptional regulator, AsnC family, at 5,187,086 to 5,187,577
_4335
Psyr_4336: Psyr_4336 - ribosomal large subunit pseudouridine synthase E, at 5,187,591 to 5,188,223
_4336
Psyr_4337: Psyr_4337 - AMP nucleosidase, at 5,188,303 to 5,189,766
_4337
Position (kb)
5187
5188
5189 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 5186.672 kb on + strand at 5186.672 kb on + strand at 5186.672 kb on + strand at 5186.672 kb on + strand at 5186.672 kb on + strand at 5186.672 kb on + strand at 5186.672 kb on + strand at 5186.672 kb on + strand at 5186.672 kb on + strand at 5186.673 kb on - strand at 5186.673 kb on - strand at 5186.673 kb on - strand at 5186.673 kb on - strand at 5186.673 kb on - strand at 5186.673 kb on - strand at 5186.673 kb on - strand at 5186.673 kb on - strand at 5186.673 kb on - strand at 5186.673 kb on - strand at 5186.716 kb on + strand at 5186.717 kb on - strand at 5186.717 kb on - strand at 5186.738 kb on - strand at 5186.738 kb on - strand at 5186.738 kb on - strand at 5186.738 kb on - strand at 5186.738 kb on - strand at 5186.747 kb on + strand at 5186.816 kb on + strand at 5186.816 kb on + strand at 5186.816 kb on + strand at 5186.816 kb on + strand at 5186.816 kb on + strand at 5186.817 kb on - strand at 5186.817 kb on - strand at 5186.817 kb on - strand at 5186.817 kb on - strand at 5186.817 kb on - strand at 5186.817 kb on - strand at 5186.875 kb on + strand at 5186.875 kb on + strand at 5186.875 kb on + strand at 5186.875 kb on + strand at 5186.875 kb on + strand at 5186.875 kb on + strand at 5186.875 kb on + strand at 5186.875 kb on + strand at 5186.875 kb on + strand at 5186.875 kb on + strand at 5186.875 kb on + strand at 5186.875 kb on + strand at 5186.875 kb on + strand at 5186.875 kb on + strand at 5186.875 kb on + strand at 5186.875 kb on + strand at 5186.875 kb on + strand at 5186.875 kb on + strand at 5186.876 kb on - strand at 5186.876 kb on - strand at 5186.876 kb on - strand at 5186.876 kb on - strand at 5186.876 kb on - strand at 5186.879 kb on + strand at 5186.879 kb on + strand at 5186.880 kb on - strand at 5186.880 kb on - strand at 5186.880 kb on - strand at 5186.880 kb on - strand at 5186.976 kb on + strand at 5186.977 kb on - strand at 5187.034 kb on - strand at 5187.160 kb on - strand, within Psyr_4335 at 5187.243 kb on - strand, within Psyr_4335 at 5187.302 kb on + strand, within Psyr_4335 at 5187.303 kb on - strand, within Psyr_4335 at 5187.420 kb on - strand, within Psyr_4335 at 5187.922 kb on + strand, within Psyr_4336 at 5187.923 kb on - strand, within Psyr_4336 at 5187.923 kb on - strand, within Psyr_4336 at 5188.030 kb on + strand, within Psyr_4336 at 5188.030 kb on + strand, within Psyr_4336 at 5188.031 kb on - strand, within Psyr_4336 at 5188.031 kb on - strand, within Psyr_4336 at 5188.279 kb on + strand at 5188.279 kb on + strand at 5188.280 kb on - strand at 5188.280 kb on - strand at 5188.280 kb on - strand at 5188.287 kb on + strand at 5188.288 kb on - strand at 5188.288 kb on - strand at 5188.288 kb on - strand at 5188.424 kb on + strand at 5188.424 kb on + strand at 5188.425 kb on - strand at 5188.425 kb on - strand at 5188.614 kb on + strand, within Psyr_4337 at 5188.615 kb on - strand, within Psyr_4337 at 5188.625 kb on + strand, within Psyr_4337 at 5188.625 kb on + strand, within Psyr_4337 at 5188.626 kb on - strand, within Psyr_4337 at 5188.626 kb on - strand, within Psyr_4337 at 5188.626 kb on - strand, within Psyr_4337 at 5188.787 kb on + strand, within Psyr_4337 at 5188.787 kb on + strand, within Psyr_4337 at 5188.787 kb on + strand, within Psyr_4337 at 5188.787 kb on + strand, within Psyr_4337 at 5188.787 kb on + strand, within Psyr_4337 at 5188.788 kb on - strand, within Psyr_4337 at 5188.788 kb on - strand, within Psyr_4337 at 5188.788 kb on - strand, within Psyr_4337 at 5188.788 kb on - strand, within Psyr_4337 at 5188.788 kb on - strand, within Psyr_4337 at 5188.788 kb on - strand, within Psyr_4337 at 5188.788 kb on - strand, within Psyr_4337 at 5188.788 kb on - strand, within Psyr_4337 at 5188.876 kb on + strand, within Psyr_4337 at 5188.876 kb on + strand, within Psyr_4337 at 5189.051 kb on - strand, within Psyr_4337 at 5189.135 kb on + strand, within Psyr_4337 at 5189.136 kb on - strand, within Psyr_4337 at 5189.136 kb on - strand, within Psyr_4337 at 5189.136 kb on - strand, within Psyr_4337 at 5189.144 kb on + strand, within Psyr_4337 at 5189.144 kb on + strand, within Psyr_4337 at 5189.144 kb on + strand, within Psyr_4337 at 5189.145 kb on - strand, within Psyr_4337 at 5189.145 kb on - strand, within Psyr_4337 at 5189.145 kb on - strand, within Psyr_4337 at 5189.145 kb on - strand, within Psyr_4337 at 5189.145 kb on - strand, within Psyr_4337 at 5189.170 kb on + strand, within Psyr_4337 at 5189.170 kb on + strand, within Psyr_4337 at 5189.170 kb on + strand, within Psyr_4337 at 5189.171 kb on - strand, within Psyr_4337 at 5189.171 kb on - strand, within Psyr_4337 at 5189.171 kb on - strand, within Psyr_4337 at 5189.171 kb on - strand, within Psyr_4337 at 5189.171 kb on - strand, within Psyr_4337 at 5189.171 kb on - strand, within Psyr_4337 at 5189.195 kb on + strand, within Psyr_4337 at 5189.195 kb on + strand, within Psyr_4337 at 5189.195 kb on + strand, within Psyr_4337 at 5189.195 kb on + strand, within Psyr_4337 at 5189.195 kb on + strand, within Psyr_4337 at 5189.196 kb on - strand, within Psyr_4337 at 5189.196 kb on - strand, within Psyr_4337 at 5189.196 kb on - strand, within Psyr_4337 at 5189.196 kb on - strand, within Psyr_4337 at 5189.196 kb on - strand, within Psyr_4337
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 5,186,672 + -3.0 5,186,672 + -0.7 5,186,672 + +2.2 5,186,672 + -2.1 5,186,672 + -3.5 5,186,672 + -0.7 5,186,672 + -0.4 5,186,672 + -0.1 5,186,672 + +2.5 5,186,673 - +0.2 5,186,673 - +1.2 5,186,673 - -0.5 5,186,673 - +0.4 5,186,673 - +1.3 5,186,673 - +2.2 5,186,673 - +2.5 5,186,673 - -1.0 5,186,673 - +3.0 5,186,673 - -2.0 5,186,716 + +4.0 5,186,717 - +0.2 5,186,717 - +1.2 5,186,738 - -0.8 5,186,738 - +3.2 5,186,738 - +2.5 5,186,738 - +1.2 5,186,738 - -0.7 5,186,747 + -1.0 5,186,816 + -0.8 5,186,816 + -0.9 5,186,816 + -1.2 5,186,816 + +1.7 5,186,816 + -0.5 5,186,817 - -0.4 5,186,817 - -1.3 5,186,817 - +4.1 5,186,817 - -0.1 5,186,817 - -1.9 5,186,817 - -0.6 5,186,875 + -2.0 5,186,875 + +1.7 5,186,875 + -4.5 5,186,875 + -1.4 5,186,875 + +0.3 5,186,875 + +0.4 5,186,875 + +0.4 5,186,875 + +0.7 5,186,875 + +0.6 5,186,875 + -1.0 5,186,875 + -3.1 5,186,875 + +0.7 5,186,875 + -0.4 5,186,875 + -0.3 5,186,875 + -0.4 5,186,875 + -1.2 5,186,875 + +3.5 5,186,875 + +0.2 5,186,876 - -0.2 5,186,876 - +0.6 5,186,876 - +0.2 5,186,876 - +2.5 5,186,876 - +3.1 5,186,879 + +1.7 5,186,879 + +3.3 5,186,880 - +3.5 5,186,880 - +3.0 5,186,880 - +3.3 5,186,880 - +4.0 5,186,976 + -0.7 5,186,977 - +2.5 5,187,034 - +3.9 5,187,160 - Psyr_4335 0.15 +4.0 5,187,243 - Psyr_4335 0.32 +1.2 5,187,302 + Psyr_4335 0.44 +1.7 5,187,303 - Psyr_4335 0.44 +2.7 5,187,420 - Psyr_4335 0.68 -1.9 5,187,922 + Psyr_4336 0.52 +1.7 5,187,923 - Psyr_4336 0.52 +1.2 5,187,923 - Psyr_4336 0.52 +2.7 5,188,030 + Psyr_4336 0.69 -1.1 5,188,030 + Psyr_4336 0.69 -1.2 5,188,031 - Psyr_4336 0.70 +2.2 5,188,031 - Psyr_4336 0.70 -3.0 5,188,279 + +4.7 5,188,279 + +0.2 5,188,280 - -0.1 5,188,280 - +0.9 5,188,280 - +0.5 5,188,287 + +2.2 5,188,288 - -0.3 5,188,288 - +1.9 5,188,288 - +0.2 5,188,424 + +0.6 5,188,424 + +0.1 5,188,425 - +4.0 5,188,425 - +4.4 5,188,614 + Psyr_4337 0.21 -0.7 5,188,615 - Psyr_4337 0.21 -0.9 5,188,625 + Psyr_4337 0.22 -0.5 5,188,625 + Psyr_4337 0.22 +4.5 5,188,626 - Psyr_4337 0.22 +0.2 5,188,626 - Psyr_4337 0.22 +1.3 5,188,626 - Psyr_4337 0.22 +3.6 5,188,787 + Psyr_4337 0.33 -1.7 5,188,787 + Psyr_4337 0.33 -0.5 5,188,787 + Psyr_4337 0.33 +4.2 5,188,787 + Psyr_4337 0.33 -0.6 5,188,787 + Psyr_4337 0.33 +3.9 5,188,788 - Psyr_4337 0.33 -2.3 5,188,788 - Psyr_4337 0.33 +2.7 5,188,788 - Psyr_4337 0.33 +1.4 5,188,788 - Psyr_4337 0.33 +3.7 5,188,788 - Psyr_4337 0.33 -0.1 5,188,788 - Psyr_4337 0.33 +3.3 5,188,788 - Psyr_4337 0.33 -2.4 5,188,788 - Psyr_4337 0.33 +1.7 5,188,876 + Psyr_4337 0.39 -0.2 5,188,876 + Psyr_4337 0.39 +3.7 5,189,051 - Psyr_4337 0.51 -0.6 5,189,135 + Psyr_4337 0.57 -0.3 5,189,136 - Psyr_4337 0.57 +3.5 5,189,136 - Psyr_4337 0.57 -0.1 5,189,136 - Psyr_4337 0.57 +0.2 5,189,144 + Psyr_4337 0.57 -0.6 5,189,144 + Psyr_4337 0.57 +0.7 5,189,144 + Psyr_4337 0.57 +0.2 5,189,145 - Psyr_4337 0.58 +5.2 5,189,145 - Psyr_4337 0.58 -1.1 5,189,145 - Psyr_4337 0.58 -0.8 5,189,145 - Psyr_4337 0.58 -1.4 5,189,145 - Psyr_4337 0.58 -1.7 5,189,170 + Psyr_4337 0.59 +1.6 5,189,170 + Psyr_4337 0.59 -1.4 5,189,170 + Psyr_4337 0.59 -0.5 5,189,171 - Psyr_4337 0.59 +4.6 5,189,171 - Psyr_4337 0.59 -2.8 5,189,171 - Psyr_4337 0.59 -0.6 5,189,171 - Psyr_4337 0.59 +0.4 5,189,171 - Psyr_4337 0.59 +0.6 5,189,171 - Psyr_4337 0.59 -1.9 5,189,195 + Psyr_4337 0.61 -1.4 5,189,195 + Psyr_4337 0.61 -0.5 5,189,195 + Psyr_4337 0.61 +4.1 5,189,195 + Psyr_4337 0.61 -1.1 5,189,195 + Psyr_4337 0.61 +1.2 5,189,196 - Psyr_4337 0.61 -0.6 5,189,196 - Psyr_4337 0.61 +2.7 5,189,196 - Psyr_4337 0.61 +0.4 5,189,196 - Psyr_4337 0.61 +1.1 5,189,196 - Psyr_4337 0.61 -0.6
Or see this region's nucleotide sequence