Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4328 and Psyr_4329 are separated by 236 nucleotides Psyr_4329 and Psyr_4330 are separated by 9 nucleotides
Psyr_4328: Psyr_4328 - Protein of unknown function DUF633, at 5,180,458 to 5,181,159
_4328
Psyr_4329: Psyr_4329 - conserved hypothetical protein, at 5,181,396 to 5,181,935
_4329
Psyr_4330: Psyr_4330 - conserved domain protein, at 5,181,945 to 5,182,838
_4330
Position (kb)
5181
5182 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 5180.404 kb on + strand at 5180.878 kb on + strand, within Psyr_4328 at 5180.962 kb on + strand, within Psyr_4328 at 5180.962 kb on + strand, within Psyr_4328 at 5180.963 kb on - strand, within Psyr_4328 at 5180.963 kb on - strand, within Psyr_4328 at 5180.963 kb on - strand, within Psyr_4328 at 5180.963 kb on - strand, within Psyr_4328 at 5180.963 kb on - strand, within Psyr_4328 at 5180.963 kb on - strand, within Psyr_4328 at 5180.980 kb on + strand, within Psyr_4328 at 5180.980 kb on + strand, within Psyr_4328 at 5180.981 kb on - strand, within Psyr_4328 at 5180.981 kb on - strand, within Psyr_4328 at 5181.217 kb on + strand at 5181.227 kb on + strand at 5181.227 kb on + strand at 5181.227 kb on + strand at 5181.227 kb on + strand at 5181.227 kb on + strand at 5181.227 kb on + strand at 5181.228 kb on - strand at 5181.228 kb on - strand at 5181.228 kb on - strand at 5181.228 kb on - strand at 5181.348 kb on + strand at 5181.368 kb on + strand at 5181.368 kb on + strand at 5181.368 kb on + strand at 5181.368 kb on + strand at 5181.368 kb on + strand at 5181.368 kb on + strand at 5181.368 kb on + strand at 5181.369 kb on - strand at 5181.369 kb on - strand at 5181.369 kb on - strand at 5181.369 kb on - strand at 5181.369 kb on - strand at 5181.822 kb on + strand, within Psyr_4329 at 5181.822 kb on + strand, within Psyr_4329 at 5181.822 kb on + strand, within Psyr_4329 at 5181.823 kb on - strand, within Psyr_4329 at 5181.841 kb on + strand, within Psyr_4329 at 5181.841 kb on + strand, within Psyr_4329 at 5181.841 kb on + strand, within Psyr_4329 at 5181.842 kb on - strand, within Psyr_4329 at 5181.842 kb on - strand, within Psyr_4329 at 5181.928 kb on + strand at 5181.928 kb on + strand at 5181.928 kb on + strand at 5181.928 kb on + strand at 5181.929 kb on - strand at 5181.929 kb on - strand at 5181.929 kb on - strand at 5181.929 kb on - strand at 5181.929 kb on - strand at 5181.929 kb on - strand at 5181.929 kb on - strand at 5182.174 kb on + strand, within Psyr_4330 at 5182.174 kb on + strand, within Psyr_4330 at 5182.174 kb on + strand, within Psyr_4330 at 5182.174 kb on + strand, within Psyr_4330 at 5182.175 kb on - strand, within Psyr_4330 at 5182.175 kb on - strand, within Psyr_4330 at 5182.318 kb on + strand, within Psyr_4330 at 5182.319 kb on - strand, within Psyr_4330 at 5182.327 kb on + strand, within Psyr_4330 at 5182.327 kb on + strand, within Psyr_4330 at 5182.330 kb on + strand, within Psyr_4330 at 5182.330 kb on + strand, within Psyr_4330 at 5182.331 kb on - strand, within Psyr_4330 at 5182.782 kb on + strand at 5182.782 kb on + strand at 5182.783 kb on - strand at 5182.783 kb on - strand at 5182.783 kb on - strand at 5182.853 kb on + strand at 5182.853 kb on + strand at 5182.854 kb on - strand at 5182.866 kb on + strand at 5182.867 kb on - strand at 5182.872 kb on + strand at 5182.872 kb on + strand at 5182.872 kb on + strand at 5182.873 kb on - strand at 5182.873 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 5,180,404 + +2.2 5,180,878 + Psyr_4328 0.60 -1.3 5,180,962 + Psyr_4328 0.72 +1.2 5,180,962 + Psyr_4328 0.72 -0.2 5,180,963 - Psyr_4328 0.72 -0.6 5,180,963 - Psyr_4328 0.72 -2.5 5,180,963 - Psyr_4328 0.72 +3.0 5,180,963 - Psyr_4328 0.72 -1.4 5,180,963 - Psyr_4328 0.72 +3.0 5,180,963 - Psyr_4328 0.72 -2.0 5,180,980 + Psyr_4328 0.74 +3.0 5,180,980 + Psyr_4328 0.74 -1.3 5,180,981 - Psyr_4328 0.75 -0.8 5,180,981 - Psyr_4328 0.75 +0.2 5,181,217 + +4.2 5,181,227 + +3.2 5,181,227 + -1.8 5,181,227 + -0.8 5,181,227 + +1.3 5,181,227 + -2.8 5,181,227 + +4.6 5,181,228 - -0.7 5,181,228 - +0.2 5,181,228 - +3.2 5,181,228 - -0.8 5,181,348 + +1.8 5,181,368 + -0.8 5,181,368 + +2.5 5,181,368 + -2.5 5,181,368 + +0.6 5,181,368 + +4.2 5,181,368 + +1.9 5,181,368 + -0.8 5,181,369 - -1.0 5,181,369 - +0.0 5,181,369 - +0.7 5,181,369 - +3.9 5,181,369 - +0.0 5,181,822 + Psyr_4329 0.79 -0.7 5,181,822 + Psyr_4329 0.79 +3.5 5,181,822 + Psyr_4329 0.79 -1.0 5,181,823 - Psyr_4329 0.79 +0.2 5,181,841 + Psyr_4329 0.82 -1.4 5,181,841 + Psyr_4329 0.82 +1.6 5,181,841 + Psyr_4329 0.82 -0.3 5,181,842 - Psyr_4329 0.83 +0.1 5,181,842 - Psyr_4329 0.83 -1.0 5,181,928 + -0.7 5,181,928 + -0.8 5,181,928 + +4.3 5,181,928 + +2.5 5,181,929 - +2.5 5,181,929 - -0.8 5,181,929 - +1.2 5,181,929 - +2.2 5,181,929 - +3.0 5,181,929 - -0.2 5,181,929 - +0.6 5,182,174 + Psyr_4330 0.26 +0.4 5,182,174 + Psyr_4330 0.26 +0.2 5,182,174 + Psyr_4330 0.26 -0.4 5,182,174 + Psyr_4330 0.26 -0.3 5,182,175 - Psyr_4330 0.26 -0.1 5,182,175 - Psyr_4330 0.26 +3.5 5,182,318 + Psyr_4330 0.42 -1.0 5,182,319 - Psyr_4330 0.42 -0.7 5,182,327 + Psyr_4330 0.43 -0.4 5,182,327 + Psyr_4330 0.43 +3.0 5,182,330 + Psyr_4330 0.43 +3.3 5,182,330 + Psyr_4330 0.43 +0.1 5,182,331 - Psyr_4330 0.43 +2.5 5,182,782 + -0.8 5,182,782 + -1.6 5,182,783 - -2.2 5,182,783 - -0.6 5,182,783 - -1.2 5,182,853 + -1.2 5,182,853 + +3.0 5,182,854 - -1.1 5,182,866 + -1.4 5,182,867 - -1.5 5,182,872 + +1.8 5,182,872 + +4.1 5,182,872 + -1.2 5,182,873 - -1.0 5,182,873 - -3.5
Or see this region's nucleotide sequence