Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4315 and Psyr_4316 are separated by 236 nucleotides Psyr_4316 and Psyr_4317 are separated by 74 nucleotides
Psyr_4315: Psyr_4315 - Asparagine synthase, glutamine-hydrolyzing, at 5,164,033 to 5,166,009
_4315
Psyr_4316: Psyr_4316 - conserved hypothetical protein, at 5,166,246 to 5,166,491
_4316
Psyr_4317: Psyr_4317 - Probable taurine catabolism dioxygenase-like protein, at 5,166,566 to 5,167,579
_4317
Position (kb) 5166
5167 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 5165.348 kb on + strand, within Psyr_4315 at 5165.348 kb on + strand, within Psyr_4315 at 5165.348 kb on + strand, within Psyr_4315 at 5165.348 kb on + strand, within Psyr_4315 at 5165.348 kb on + strand, within Psyr_4315 at 5165.348 kb on + strand, within Psyr_4315 at 5165.351 kb on + strand, within Psyr_4315 at 5165.351 kb on + strand, within Psyr_4315 at 5165.351 kb on + strand, within Psyr_4315 at 5165.351 kb on + strand, within Psyr_4315 at 5165.351 kb on + strand, within Psyr_4315 at 5165.352 kb on - strand, within Psyr_4315 at 5165.352 kb on - strand, within Psyr_4315 at 5165.564 kb on + strand, within Psyr_4315 at 5165.565 kb on - strand, within Psyr_4315 at 5165.565 kb on - strand, within Psyr_4315 at 5165.565 kb on - strand, within Psyr_4315 at 5165.565 kb on - strand, within Psyr_4315 at 5165.601 kb on + strand, within Psyr_4315 at 5165.602 kb on - strand, within Psyr_4315 at 5165.602 kb on - strand, within Psyr_4315 at 5165.602 kb on - strand, within Psyr_4315 at 5165.602 kb on - strand, within Psyr_4315 at 5165.602 kb on - strand, within Psyr_4315 at 5165.605 kb on + strand, within Psyr_4315 at 5165.628 kb on - strand, within Psyr_4315 at 5165.758 kb on + strand, within Psyr_4315 at 5165.758 kb on + strand, within Psyr_4315 at 5165.768 kb on + strand, within Psyr_4315 at 5165.768 kb on + strand, within Psyr_4315 at 5165.769 kb on - strand, within Psyr_4315 at 5165.769 kb on - strand, within Psyr_4315 at 5165.769 kb on - strand, within Psyr_4315 at 5165.773 kb on - strand, within Psyr_4315 at 5165.773 kb on - strand, within Psyr_4315 at 5165.774 kb on + strand, within Psyr_4315 at 5165.774 kb on + strand, within Psyr_4315 at 5165.774 kb on + strand, within Psyr_4315 at 5165.775 kb on - strand, within Psyr_4315 at 5165.775 kb on - strand, within Psyr_4315 at 5165.775 kb on - strand, within Psyr_4315 at 5165.832 kb on + strand at 5165.833 kb on - strand at 5165.833 kb on - strand at 5165.853 kb on + strand at 5165.854 kb on - strand at 5165.857 kb on + strand at 5165.858 kb on - strand at 5165.887 kb on - strand at 5165.894 kb on + strand at 5165.894 kb on + strand at 5165.895 kb on - strand at 5165.895 kb on - strand at 5165.895 kb on - strand at 5165.895 kb on - strand at 5166.181 kb on - strand at 5166.206 kb on + strand at 5166.207 kb on - strand at 5166.207 kb on - strand at 5166.282 kb on + strand, within Psyr_4316 at 5166.287 kb on + strand, within Psyr_4316 at 5166.321 kb on - strand, within Psyr_4316 at 5166.369 kb on - strand, within Psyr_4316 at 5166.379 kb on + strand, within Psyr_4316 at 5166.430 kb on - strand, within Psyr_4316 at 5166.431 kb on + strand, within Psyr_4316 at 5166.494 kb on - strand at 5166.544 kb on - strand at 5166.563 kb on - strand at 5166.563 kb on - strand at 5166.610 kb on - strand at 5166.656 kb on - strand at 5166.667 kb on - strand at 5166.667 kb on - strand at 5166.670 kb on - strand, within Psyr_4317 at 5166.691 kb on + strand, within Psyr_4317 at 5166.691 kb on + strand, within Psyr_4317 at 5166.805 kb on - strand, within Psyr_4317 at 5166.901 kb on - strand, within Psyr_4317 at 5166.947 kb on + strand, within Psyr_4317 at 5167.045 kb on + strand, within Psyr_4317 at 5167.048 kb on - strand, within Psyr_4317 at 5167.107 kb on + strand, within Psyr_4317 at 5167.108 kb on - strand, within Psyr_4317 at 5167.108 kb on - strand, within Psyr_4317 at 5167.108 kb on - strand, within Psyr_4317 at 5167.109 kb on + strand, within Psyr_4317 at 5167.110 kb on - strand, within Psyr_4317 at 5167.117 kb on + strand, within Psyr_4317 at 5167.118 kb on - strand, within Psyr_4317 at 5167.184 kb on - strand, within Psyr_4317 at 5167.295 kb on - strand, within Psyr_4317 at 5167.358 kb on + strand, within Psyr_4317 at 5167.358 kb on + strand, within Psyr_4317 at 5167.358 kb on + strand, within Psyr_4317 at 5167.358 kb on + strand, within Psyr_4317
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 5,165,348 + Psyr_4315 0.67 +0.2 5,165,348 + Psyr_4315 0.67 -0.4 5,165,348 + Psyr_4315 0.67 -1.2 5,165,348 + Psyr_4315 0.67 -0.8 5,165,348 + Psyr_4315 0.67 -3.1 5,165,348 + Psyr_4315 0.67 -0.7 5,165,351 + Psyr_4315 0.67 -2.4 5,165,351 + Psyr_4315 0.67 -1.3 5,165,351 + Psyr_4315 0.67 -0.9 5,165,351 + Psyr_4315 0.67 -0.6 5,165,351 + Psyr_4315 0.67 -0.3 5,165,352 - Psyr_4315 0.67 +1.2 5,165,352 - Psyr_4315 0.67 -0.1 5,165,564 + Psyr_4315 0.77 -3.6 5,165,565 - Psyr_4315 0.77 +0.2 5,165,565 - Psyr_4315 0.77 +0.4 5,165,565 - Psyr_4315 0.77 +0.2 5,165,565 - Psyr_4315 0.77 -2.0 5,165,601 + Psyr_4315 0.79 +0.2 5,165,602 - Psyr_4315 0.79 -1.0 5,165,602 - Psyr_4315 0.79 +0.8 5,165,602 - Psyr_4315 0.79 +2.2 5,165,602 - Psyr_4315 0.79 +3.6 5,165,602 - Psyr_4315 0.79 +3.0 5,165,605 + Psyr_4315 0.80 +1.2 5,165,628 - Psyr_4315 0.81 +2.2 5,165,758 + Psyr_4315 0.87 +0.2 5,165,758 + Psyr_4315 0.87 -1.9 5,165,768 + Psyr_4315 0.88 -2.0 5,165,768 + Psyr_4315 0.88 -1.4 5,165,769 - Psyr_4315 0.88 +0.2 5,165,769 - Psyr_4315 0.88 -0.3 5,165,769 - Psyr_4315 0.88 +1.6 5,165,773 - Psyr_4315 0.88 +2.2 5,165,773 - Psyr_4315 0.88 +3.7 5,165,774 + Psyr_4315 0.88 +3.2 5,165,774 + Psyr_4315 0.88 +0.5 5,165,774 + Psyr_4315 0.88 +2.2 5,165,775 - Psyr_4315 0.88 -1.3 5,165,775 - Psyr_4315 0.88 +1.2 5,165,775 - Psyr_4315 0.88 +2.5 5,165,832 + -1.5 5,165,833 - -3.5 5,165,833 - -2.2 5,165,853 + +2.5 5,165,854 - +0.7 5,165,857 + -2.0 5,165,858 - +0.8 5,165,887 - +3.5 5,165,894 + -0.1 5,165,894 + -4.2 5,165,895 - +0.2 5,165,895 - +3.0 5,165,895 - -0.6 5,165,895 - -0.1 5,166,181 - -1.0 5,166,206 + +2.2 5,166,207 - -0.4 5,166,207 - +3.5 5,166,282 + Psyr_4316 0.15 -2.4 5,166,287 + Psyr_4316 0.17 -1.8 5,166,321 - Psyr_4316 0.30 -1.4 5,166,369 - Psyr_4316 0.50 +2.5 5,166,379 + Psyr_4316 0.54 -2.0 5,166,430 - Psyr_4316 0.75 +4.6 5,166,431 + Psyr_4316 0.75 +2.5 5,166,494 - +3.9 5,166,544 - -0.6 5,166,563 - +2.2 5,166,563 - -1.6 5,166,610 - +2.5 5,166,656 - -3.5 5,166,667 - -0.1 5,166,667 - +0.2 5,166,670 - Psyr_4317 0.10 -1.1 5,166,691 + Psyr_4317 0.12 +3.2 5,166,691 + Psyr_4317 0.12 -1.4 5,166,805 - Psyr_4317 0.24 -1.3 5,166,901 - Psyr_4317 0.33 +1.6 5,166,947 + Psyr_4317 0.38 -1.8 5,167,045 + Psyr_4317 0.47 +0.2 5,167,048 - Psyr_4317 0.48 +5.2 5,167,107 + Psyr_4317 0.53 +0.2 5,167,108 - Psyr_4317 0.53 -0.3 5,167,108 - Psyr_4317 0.53 +1.2 5,167,108 - Psyr_4317 0.53 -2.8 5,167,109 + Psyr_4317 0.54 +2.2 5,167,110 - Psyr_4317 0.54 +3.5 5,167,117 + Psyr_4317 0.54 -2.6 5,167,118 - Psyr_4317 0.54 -3.3 5,167,184 - Psyr_4317 0.61 +2.5 5,167,295 - Psyr_4317 0.72 -2.6 5,167,358 + Psyr_4317 0.78 +1.7 5,167,358 + Psyr_4317 0.78 +2.5 5,167,358 + Psyr_4317 0.78 -2.8 5,167,358 + Psyr_4317 0.78 +2.2
Or see this region's nucleotide sequence