Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4302 and Psyr_4303 are separated by 197 nucleotides Psyr_4303 and Psyr_4304 are separated by 33 nucleotides Psyr_4304 and Psyr_4305 are separated by 184 nucleotides Psyr_4305 and Psyr_4306 are separated by 104 nucleotides
Psyr_4302: Psyr_4302 - conserved hypothetical protein, at 5,128,171 to 5,128,875
_4302
Psyr_4303: Psyr_4303 - Protein of unknown function UPF0259, at 5,129,073 to 5,129,735
_4303
Psyr_4304: Psyr_4304 - Endonuclease/exonuclease/phosphatase, at 5,129,769 to 5,130,911
_4304
Psyr_4305: Psyr_4305 - conserved hypothetical protein, at 5,131,096 to 5,131,344
_4305
Psyr_4306: Psyr_4306 - conserved hypothetical protein, at 5,131,449 to 5,131,646
_4306
Position (kb) 5129
5130
5131 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 5129.035 kb on + strand at 5129.054 kb on + strand at 5129.054 kb on + strand at 5129.066 kb on + strand at 5129.066 kb on + strand at 5129.066 kb on + strand at 5129.066 kb on + strand at 5129.066 kb on + strand at 5129.066 kb on + strand at 5129.067 kb on - strand at 5129.067 kb on - strand at 5129.067 kb on - strand at 5129.067 kb on - strand at 5129.067 kb on - strand at 5129.067 kb on - strand at 5129.067 kb on - strand at 5129.106 kb on + strand at 5129.107 kb on - strand at 5129.107 kb on - strand at 5129.121 kb on - strand at 5129.125 kb on + strand at 5129.126 kb on - strand at 5129.126 kb on - strand at 5129.126 kb on - strand at 5129.126 kb on - strand at 5129.126 kb on - strand at 5129.255 kb on + strand, within Psyr_4303 at 5129.255 kb on + strand, within Psyr_4303 at 5129.255 kb on + strand, within Psyr_4303 at 5129.255 kb on + strand, within Psyr_4303 at 5129.255 kb on + strand, within Psyr_4303 at 5129.255 kb on + strand, within Psyr_4303 at 5129.255 kb on + strand, within Psyr_4303 at 5129.256 kb on - strand, within Psyr_4303 at 5129.256 kb on - strand, within Psyr_4303 at 5129.256 kb on - strand, within Psyr_4303 at 5129.256 kb on - strand, within Psyr_4303 at 5129.259 kb on + strand, within Psyr_4303 at 5129.260 kb on - strand, within Psyr_4303 at 5129.311 kb on + strand, within Psyr_4303 at 5129.311 kb on + strand, within Psyr_4303 at 5129.311 kb on + strand, within Psyr_4303 at 5129.311 kb on + strand, within Psyr_4303 at 5129.311 kb on + strand, within Psyr_4303 at 5129.311 kb on + strand, within Psyr_4303 at 5129.311 kb on + strand, within Psyr_4303 at 5129.311 kb on + strand, within Psyr_4303 at 5129.311 kb on + strand, within Psyr_4303 at 5129.311 kb on + strand, within Psyr_4303 at 5129.312 kb on - strand, within Psyr_4303 at 5129.312 kb on - strand, within Psyr_4303 at 5129.312 kb on - strand, within Psyr_4303 at 5129.312 kb on - strand, within Psyr_4303 at 5129.312 kb on - strand, within Psyr_4303 at 5129.312 kb on - strand, within Psyr_4303 at 5129.312 kb on - strand, within Psyr_4303 at 5129.312 kb on - strand, within Psyr_4303 at 5129.312 kb on - strand, within Psyr_4303 at 5129.312 kb on - strand, within Psyr_4303 at 5129.312 kb on - strand at 5129.326 kb on + strand, within Psyr_4303 at 5129.326 kb on + strand, within Psyr_4303 at 5129.326 kb on + strand, within Psyr_4303 at 5129.326 kb on + strand, within Psyr_4303 at 5129.554 kb on + strand, within Psyr_4303 at 5129.565 kb on + strand, within Psyr_4303 at 5129.565 kb on + strand, within Psyr_4303 at 5129.565 kb on + strand, within Psyr_4303 at 5129.565 kb on + strand, within Psyr_4303 at 5129.565 kb on + strand, within Psyr_4303 at 5129.565 kb on + strand, within Psyr_4303 at 5129.565 kb on + strand, within Psyr_4303 at 5129.565 kb on + strand, within Psyr_4303 at 5129.566 kb on - strand, within Psyr_4303 at 5129.566 kb on - strand, within Psyr_4303 at 5129.566 kb on - strand, within Psyr_4303 at 5129.602 kb on - strand, within Psyr_4303 at 5129.602 kb on - strand, within Psyr_4303 at 5129.830 kb on + strand at 5129.831 kb on - strand at 5129.836 kb on + strand at 5129.836 kb on + strand at 5129.836 kb on + strand at 5129.836 kb on + strand at 5129.836 kb on + strand at 5129.837 kb on - strand at 5129.837 kb on - strand at 5129.837 kb on - strand at 5129.837 kb on - strand at 5129.923 kb on + strand, within Psyr_4304 at 5129.923 kb on + strand, within Psyr_4304 at 5129.923 kb on + strand, within Psyr_4304 at 5129.924 kb on - strand, within Psyr_4304 at 5129.924 kb on - strand, within Psyr_4304 at 5129.924 kb on - strand, within Psyr_4304 at 5130.027 kb on + strand, within Psyr_4304 at 5130.027 kb on + strand, within Psyr_4304 at 5130.190 kb on - strand, within Psyr_4304 at 5130.190 kb on - strand, within Psyr_4304 at 5130.234 kb on + strand, within Psyr_4304 at 5130.290 kb on + strand, within Psyr_4304 at 5130.290 kb on + strand, within Psyr_4304 at 5130.290 kb on + strand, within Psyr_4304 at 5130.290 kb on + strand, within Psyr_4304 at 5130.290 kb on + strand, within Psyr_4304 at 5130.291 kb on - strand, within Psyr_4304 at 5130.291 kb on - strand, within Psyr_4304 at 5130.429 kb on + strand, within Psyr_4304 at 5130.429 kb on + strand, within Psyr_4304 at 5130.429 kb on + strand, within Psyr_4304 at 5130.430 kb on - strand, within Psyr_4304 at 5130.430 kb on - strand, within Psyr_4304 at 5130.430 kb on - strand, within Psyr_4304 at 5130.673 kb on + strand, within Psyr_4304 at 5130.673 kb on + strand, within Psyr_4304 at 5130.674 kb on - strand, within Psyr_4304 at 5130.674 kb on - strand, within Psyr_4304 at 5130.684 kb on + strand, within Psyr_4304 at 5130.685 kb on - strand, within Psyr_4304 at 5130.792 kb on + strand, within Psyr_4304 at 5130.792 kb on + strand, within Psyr_4304 at 5130.793 kb on - strand, within Psyr_4304 at 5130.793 kb on - strand, within Psyr_4304 at 5130.793 kb on - strand, within Psyr_4304 at 5130.801 kb on + strand at 5130.801 kb on + strand at 5130.801 kb on + strand at 5130.801 kb on + strand at 5130.802 kb on - strand at 5130.848 kb on + strand at 5130.848 kb on + strand at 5130.848 kb on + strand at 5130.848 kb on + strand at 5130.848 kb on + strand at 5130.848 kb on + strand at 5130.848 kb on + strand at 5130.848 kb on + strand at 5130.848 kb on + strand at 5130.848 kb on + strand at 5130.849 kb on - strand at 5130.849 kb on - strand at 5130.849 kb on - strand at 5130.849 kb on - strand at 5130.849 kb on - strand at 5130.849 kb on - strand at 5130.849 kb on - strand at 5130.849 kb on - strand at 5130.849 kb on - strand at 5130.849 kb on - strand at 5130.938 kb on + strand at 5130.997 kb on + strand at 5130.997 kb on + strand at 5130.998 kb on - strand at 5130.998 kb on - strand at 5130.998 kb on - strand at 5130.998 kb on - strand at 5131.077 kb on - strand at 5131.077 kb on - strand at 5131.119 kb on + strand at 5131.120 kb on - strand at 5131.120 kb on - strand at 5131.273 kb on - strand, within Psyr_4305 at 5131.408 kb on + strand at 5131.408 kb on + strand at 5131.408 kb on + strand at 5131.408 kb on + strand at 5131.408 kb on + strand at 5131.408 kb on + strand at 5131.409 kb on - strand at 5131.409 kb on - strand at 5131.409 kb on - strand at 5131.409 kb on - strand at 5131.409 kb on - strand at 5131.409 kb on - strand at 5131.409 kb on - strand at 5131.438 kb on + strand at 5131.438 kb on + strand at 5131.438 kb on + strand at 5131.438 kb on + strand at 5131.438 kb on + strand at 5131.438 kb on + strand at 5131.439 kb on - strand at 5131.439 kb on - strand at 5131.439 kb on - strand at 5131.439 kb on - strand at 5131.439 kb on - strand at 5131.439 kb on - strand at 5131.442 kb on + strand at 5131.443 kb on - strand at 5131.443 kb on - strand at 5131.605 kb on - strand, within Psyr_4306 at 5131.638 kb on - strand at 5131.697 kb on - strand at 5131.730 kb on - strand at 5131.730 kb on - strand at 5131.731 kb on + strand at 5131.731 kb on + strand at 5131.731 kb on + strand at 5131.732 kb on - strand at 5131.759 kb on - strand at 5131.759 kb on - strand at 5131.759 kb on - strand at 5131.759 kb on - strand at 5131.759 kb on - strand at 5131.762 kb on + strand at 5131.830 kb on - strand at 5131.830 kb on - strand at 5131.830 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 5,129,035 + -0.0 5,129,054 + -1.9 5,129,054 + +0.4 5,129,066 + +3.0 5,129,066 + -2.0 5,129,066 + +2.7 5,129,066 + +0.2 5,129,066 + +0.2 5,129,066 + -0.8 5,129,067 - -0.7 5,129,067 - +3.7 5,129,067 - -0.7 5,129,067 - +1.7 5,129,067 - +0.9 5,129,067 - +1.2 5,129,067 - +0.2 5,129,106 + +2.7 5,129,107 - +0.2 5,129,107 - +2.2 5,129,121 - +0.2 5,129,125 + +4.0 5,129,126 - +3.0 5,129,126 - -0.9 5,129,126 - +3.2 5,129,126 - +1.4 5,129,126 - -0.6 5,129,255 + Psyr_4303 0.27 -1.4 5,129,255 + Psyr_4303 0.27 +0.8 5,129,255 + Psyr_4303 0.27 +4.1 5,129,255 + Psyr_4303 0.27 -1.1 5,129,255 + Psyr_4303 0.27 -1.4 5,129,255 + Psyr_4303 0.27 -0.1 5,129,255 + Psyr_4303 0.27 +3.7 5,129,256 - Psyr_4303 0.28 +2.0 5,129,256 - Psyr_4303 0.28 -1.8 5,129,256 - Psyr_4303 0.28 +2.2 5,129,256 - Psyr_4303 0.28 +1.4 5,129,259 + Psyr_4303 0.28 +4.2 5,129,260 - Psyr_4303 0.28 -2.5 5,129,311 + Psyr_4303 0.36 -3.1 5,129,311 + Psyr_4303 0.36 +3.2 5,129,311 + Psyr_4303 0.36 +3.3 5,129,311 + Psyr_4303 0.36 +0.5 5,129,311 + Psyr_4303 0.36 +0.4 5,129,311 + Psyr_4303 0.36 +1.0 5,129,311 + Psyr_4303 0.36 +1.3 5,129,311 + Psyr_4303 0.36 -0.9 5,129,311 + Psyr_4303 0.36 -1.2 5,129,311 + Psyr_4303 0.36 -0.6 5,129,312 - Psyr_4303 0.36 +0.0 5,129,312 - Psyr_4303 0.36 -1.3 5,129,312 - Psyr_4303 0.36 -0.8 5,129,312 - Psyr_4303 0.36 -1.4 5,129,312 - Psyr_4303 0.36 +4.1 5,129,312 - Psyr_4303 0.36 -0.3 5,129,312 - Psyr_4303 0.36 +1.8 5,129,312 - Psyr_4303 0.36 +1.7 5,129,312 - Psyr_4303 0.36 -0.4 5,129,312 - Psyr_4303 0.36 -1.3 5,129,312 - +2.7 5,129,326 + Psyr_4303 0.38 +0.7 5,129,326 + Psyr_4303 0.38 +3.5 5,129,326 + Psyr_4303 0.38 +3.3 5,129,326 + Psyr_4303 0.38 -1.7 5,129,554 + Psyr_4303 0.73 -2.3 5,129,565 + Psyr_4303 0.74 -2.3 5,129,565 + Psyr_4303 0.74 +2.2 5,129,565 + Psyr_4303 0.74 -0.4 5,129,565 + Psyr_4303 0.74 -0.2 5,129,565 + Psyr_4303 0.74 +0.2 5,129,565 + Psyr_4303 0.74 -2.8 5,129,565 + Psyr_4303 0.74 +1.7 5,129,565 + Psyr_4303 0.74 -0.5 5,129,566 - Psyr_4303 0.74 -1.1 5,129,566 - Psyr_4303 0.74 -1.0 5,129,566 - Psyr_4303 0.74 -0.4 5,129,602 - Psyr_4303 0.80 +0.0 5,129,602 - Psyr_4303 0.80 +4.1 5,129,830 + -0.8 5,129,831 - -3.8 5,129,836 + +4.2 5,129,836 + +2.7 5,129,836 + +4.2 5,129,836 + -2.1 5,129,836 + +3.2 5,129,837 - -0.0 5,129,837 - +3.5 5,129,837 - -0.9 5,129,837 - -0.9 5,129,923 + Psyr_4304 0.13 -1.8 5,129,923 + Psyr_4304 0.13 -3.0 5,129,923 + Psyr_4304 0.13 +3.9 5,129,924 - Psyr_4304 0.14 -1.3 5,129,924 - Psyr_4304 0.14 +3.6 5,129,924 - Psyr_4304 0.14 +0.9 5,130,027 + Psyr_4304 0.23 +0.2 5,130,027 + Psyr_4304 0.23 -0.7 5,130,190 - Psyr_4304 0.37 +3.6 5,130,190 - Psyr_4304 0.37 -0.3 5,130,234 + Psyr_4304 0.41 -2.5 5,130,290 + Psyr_4304 0.46 +2.2 5,130,290 + Psyr_4304 0.46 +1.3 5,130,290 + Psyr_4304 0.46 -1.1 5,130,290 + Psyr_4304 0.46 +4.5 5,130,290 + Psyr_4304 0.46 +3.3 5,130,291 - Psyr_4304 0.46 -1.0 5,130,291 - Psyr_4304 0.46 +4.7 5,130,429 + Psyr_4304 0.58 +2.7 5,130,429 + Psyr_4304 0.58 +2.5 5,130,429 + Psyr_4304 0.58 -2.6 5,130,430 - Psyr_4304 0.58 -0.5 5,130,430 - Psyr_4304 0.58 -0.5 5,130,430 - Psyr_4304 0.58 -1.8 5,130,673 + Psyr_4304 0.79 +3.5 5,130,673 + Psyr_4304 0.79 +0.2 5,130,674 - Psyr_4304 0.79 +0.1 5,130,674 - Psyr_4304 0.79 +3.2 5,130,684 + Psyr_4304 0.80 +3.7 5,130,685 - Psyr_4304 0.80 -0.5 5,130,792 + Psyr_4304 0.90 +0.2 5,130,792 + Psyr_4304 0.90 +0.2 5,130,793 - Psyr_4304 0.90 -1.0 5,130,793 - Psyr_4304 0.90 +0.3 5,130,793 - Psyr_4304 0.90 +1.7 5,130,801 + +3.2 5,130,801 + +4.5 5,130,801 + -0.1 5,130,801 + +0.4 5,130,802 - +3.6 5,130,848 + +1.7 5,130,848 + +3.3 5,130,848 + +4.2 5,130,848 + -0.2 5,130,848 + -1.9 5,130,848 + -1.1 5,130,848 + +1.2 5,130,848 + -0.9 5,130,848 + +4.2 5,130,848 + -1.6 5,130,849 - +2.2 5,130,849 - +3.0 5,130,849 - -3.0 5,130,849 - +2.7 5,130,849 - -2.0 5,130,849 - -1.0 5,130,849 - -2.4 5,130,849 - -3.2 5,130,849 - +0.2 5,130,849 - +0.9 5,130,938 + -0.0 5,130,997 + -1.7 5,130,997 + +4.1 5,130,998 - +2.7 5,130,998 - -0.5 5,130,998 - +0.6 5,130,998 - +0.3 5,131,077 - +3.0 5,131,077 - -0.5 5,131,119 + +2.2 5,131,120 - -1.0 5,131,120 - -0.7 5,131,273 - Psyr_4305 0.71 -1.0 5,131,408 + -3.4 5,131,408 + +0.2 5,131,408 + -1.4 5,131,408 + +4.1 5,131,408 + +0.0 5,131,408 + +1.7 5,131,409 - -1.7 5,131,409 - -1.6 5,131,409 - -0.8 5,131,409 - -2.7 5,131,409 - +0.2 5,131,409 - +4.3 5,131,409 - -1.1 5,131,438 + +2.5 5,131,438 + +3.3 5,131,438 + +2.2 5,131,438 + +2.2 5,131,438 + +1.2 5,131,438 + -3.2 5,131,439 - +2.7 5,131,439 - -1.9 5,131,439 - +0.4 5,131,439 - -0.7 5,131,439 - +1.2 5,131,439 - +0.2 5,131,442 + -2.3 5,131,443 - +4.0 5,131,443 - -0.6 5,131,605 - Psyr_4306 0.79 -2.1 5,131,638 - +0.8 5,131,697 - -2.0 5,131,730 - +0.2 5,131,730 - -2.3 5,131,731 + -0.7 5,131,731 + +3.5 5,131,731 + +0.2 5,131,732 - -0.8 5,131,759 - +0.5 5,131,759 - -1.1 5,131,759 - -0.5 5,131,759 - -3.2 5,131,759 - +3.9 5,131,762 + -0.8 5,131,830 - +1.2 5,131,830 - +0.2 5,131,830 - +3.2
Or see this region's nucleotide sequence