Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4142 and Psyr_4143 are separated by 29 nucleotides Psyr_4143 and Psyr_4144 are separated by 8 nucleotides Psyr_4144 and Psyr_4145 overlap by 14 nucleotides Psyr_4145 and Psyr_4146 are separated by 0 nucleotides
Psyr_4142: Psyr_4142 - KpsF/GutQ, at 4,931,039 to 4,932,013
_4142
Psyr_4143: Psyr_4143 - HAD-superfamily hydrolase, subfamily IIIA:Phosphatase YrbI, at 4,932,043 to 4,932,552
_4143
Psyr_4144: Psyr_4144 - Protein of unknown function DUF1239, at 4,932,561 to 4,933,133
_4144
Psyr_4145: Psyr_4145 - OstA-like protein, at 4,933,120 to 4,933,668
_4145
Psyr_4146: Psyr_4146 - ABC transporter, at 4,933,669 to 4,934,394
_4146
Position (kb)
4932
4933
4934 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 4932.011 kb on + strand at 4932.011 kb on + strand at 4932.011 kb on + strand at 4932.011 kb on + strand at 4932.026 kb on + strand at 4932.026 kb on + strand at 4932.112 kb on + strand, within Psyr_4143 at 4932.150 kb on + strand, within Psyr_4143 at 4932.171 kb on + strand, within Psyr_4143 at 4932.334 kb on + strand, within Psyr_4143 at 4932.431 kb on + strand, within Psyr_4143 at 4932.544 kb on + strand at 4932.548 kb on + strand at 4932.548 kb on + strand at 4932.548 kb on + strand at 4932.548 kb on + strand at 4932.548 kb on + strand at 4932.550 kb on + strand at 4932.550 kb on + strand at 4932.560 kb on + strand at 4932.560 kb on + strand at 4932.560 kb on + strand at 4932.560 kb on + strand at 4932.560 kb on + strand at 4932.560 kb on + strand at 4932.560 kb on + strand at 4932.560 kb on + strand at 4932.982 kb on + strand, within Psyr_4144 at 4932.982 kb on + strand, within Psyr_4144 at 4933.108 kb on + strand at 4933.108 kb on + strand at 4933.108 kb on + strand at 4933.108 kb on + strand at 4933.108 kb on + strand at 4933.108 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 4,932,011 + -1.1 4,932,011 + -1.2 4,932,011 + +1.7 4,932,011 + +2.7 4,932,026 + -2.7 4,932,026 + -1.0 4,932,112 + Psyr_4143 0.14 +3.0 4,932,150 + Psyr_4143 0.21 -0.3 4,932,171 + Psyr_4143 0.25 -3.2 4,932,334 + Psyr_4143 0.57 +3.6 4,932,431 + Psyr_4143 0.76 -1.2 4,932,544 + -1.2 4,932,548 + +3.1 4,932,548 + -0.1 4,932,548 + -0.2 4,932,548 + +4.2 4,932,548 + +4.2 4,932,550 + +3.0 4,932,550 + +3.0 4,932,560 + +3.1 4,932,560 + +1.1 4,932,560 + -0.7 4,932,560 + +2.5 4,932,560 + -3.7 4,932,560 + -1.4 4,932,560 + +3.0 4,932,560 + +3.0 4,932,982 + Psyr_4144 0.73 -4.6 4,932,982 + Psyr_4144 0.73 +0.1 4,933,108 + -3.3 4,933,108 + +2.1 4,933,108 + -2.4 4,933,108 + -2.0 4,933,108 + -2.8 4,933,108 + -4.7
Or see this region's nucleotide sequence