Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4044 and Psyr_4045 are separated by 1 nucleotides Psyr_4045 and Psyr_4046 are separated by 89 nucleotides Psyr_4046 and Psyr_4047 overlap by 4 nucleotides
Psyr_4044: Psyr_4044 - Phospholipase D/Transphosphatidylase, at 4,828,329 to 4,829,486
_4044
Psyr_4045: Psyr_4045 - YceI, at 4,829,488 to 4,830,069
_4045
Psyr_4046: Psyr_4046 - conserved hypothetical protein, at 4,830,159 to 4,830,488
_4046
Psyr_4047: Psyr_4047 - Beta-lactamase, at 4,830,485 to 4,831,591
_4047
Position (kb)
4830
4831 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 4829.195 kb on - strand, within Psyr_4044 at 4829.359 kb on + strand, within Psyr_4044 at 4829.359 kb on + strand, within Psyr_4044 at 4829.359 kb on + strand, within Psyr_4044 at 4829.486 kb on + strand at 4829.677 kb on - strand, within Psyr_4045 at 4829.677 kb on - strand, within Psyr_4045 at 4829.678 kb on + strand, within Psyr_4045 at 4829.678 kb on + strand, within Psyr_4045 at 4829.678 kb on + strand, within Psyr_4045 at 4829.678 kb on + strand, within Psyr_4045 at 4829.678 kb on + strand, within Psyr_4045 at 4829.678 kb on + strand, within Psyr_4045 at 4829.678 kb on + strand, within Psyr_4045 at 4829.679 kb on - strand, within Psyr_4045 at 4829.679 kb on - strand, within Psyr_4045 at 4829.679 kb on - strand, within Psyr_4045 at 4829.754 kb on + strand, within Psyr_4045 at 4829.879 kb on + strand, within Psyr_4045 at 4830.000 kb on - strand, within Psyr_4045 at 4830.020 kb on - strand at 4830.060 kb on + strand at 4830.060 kb on + strand at 4830.060 kb on + strand at 4830.278 kb on - strand, within Psyr_4046 at 4830.278 kb on - strand, within Psyr_4046 at 4830.289 kb on + strand, within Psyr_4046 at 4830.289 kb on + strand, within Psyr_4046 at 4830.289 kb on + strand, within Psyr_4046 at 4830.290 kb on - strand, within Psyr_4046 at 4830.290 kb on - strand, within Psyr_4046 at 4830.290 kb on - strand, within Psyr_4046 at 4830.301 kb on + strand, within Psyr_4046 at 4830.301 kb on + strand, within Psyr_4046 at 4830.302 kb on - strand, within Psyr_4046 at 4830.325 kb on + strand, within Psyr_4046 at 4830.326 kb on - strand, within Psyr_4046 at 4830.326 kb on - strand, within Psyr_4046 at 4830.326 kb on - strand, within Psyr_4046 at 4830.326 kb on - strand, within Psyr_4046 at 4830.340 kb on + strand, within Psyr_4046 at 4830.340 kb on + strand, within Psyr_4046 at 4830.340 kb on + strand, within Psyr_4046 at 4830.341 kb on - strand, within Psyr_4046 at 4830.341 kb on - strand, within Psyr_4046 at 4830.356 kb on - strand, within Psyr_4046 at 4830.356 kb on - strand, within Psyr_4046 at 4830.546 kb on + strand at 4830.546 kb on + strand at 4830.546 kb on + strand at 4830.546 kb on + strand at 4830.546 kb on + strand at 4830.546 kb on + strand at 4830.546 kb on + strand at 4830.546 kb on + strand at 4830.546 kb on + strand at 4830.546 kb on + strand at 4830.546 kb on + strand at 4830.547 kb on - strand at 4830.547 kb on - strand at 4830.547 kb on - strand at 4830.547 kb on - strand at 4830.547 kb on - strand at 4830.547 kb on - strand at 4830.547 kb on - strand at 4830.551 kb on - strand at 4830.606 kb on + strand, within Psyr_4047 at 4830.606 kb on + strand, within Psyr_4047 at 4830.606 kb on + strand, within Psyr_4047 at 4830.606 kb on + strand, within Psyr_4047 at 4830.606 kb on + strand, within Psyr_4047 at 4830.606 kb on + strand, within Psyr_4047 at 4830.606 kb on + strand, within Psyr_4047 at 4830.606 kb on + strand, within Psyr_4047 at 4830.606 kb on + strand, within Psyr_4047 at 4830.606 kb on + strand, within Psyr_4047 at 4830.607 kb on - strand, within Psyr_4047 at 4830.607 kb on - strand, within Psyr_4047 at 4830.607 kb on - strand, within Psyr_4047 at 4830.607 kb on - strand, within Psyr_4047 at 4830.607 kb on - strand, within Psyr_4047 at 4830.689 kb on - strand, within Psyr_4047 at 4830.861 kb on + strand, within Psyr_4047 at 4830.861 kb on + strand, within Psyr_4047 at 4830.861 kb on + strand, within Psyr_4047 at 4830.861 kb on + strand, within Psyr_4047 at 4830.861 kb on + strand, within Psyr_4047 at 4830.861 kb on + strand, within Psyr_4047 at 4830.861 kb on + strand, within Psyr_4047 at 4830.861 kb on + strand, within Psyr_4047 at 4830.861 kb on + strand, within Psyr_4047 at 4830.861 kb on + strand, within Psyr_4047 at 4830.861 kb on + strand, within Psyr_4047 at 4830.862 kb on - strand, within Psyr_4047 at 4830.862 kb on - strand, within Psyr_4047 at 4830.862 kb on - strand, within Psyr_4047 at 4830.862 kb on - strand, within Psyr_4047 at 4830.862 kb on - strand, within Psyr_4047 at 4830.862 kb on - strand, within Psyr_4047 at 4830.862 kb on - strand, within Psyr_4047 at 4830.862 kb on - strand, within Psyr_4047 at 4830.862 kb on - strand, within Psyr_4047 at 4830.862 kb on - strand, within Psyr_4047 at 4830.867 kb on + strand, within Psyr_4047 at 4830.868 kb on - strand, within Psyr_4047 at 4830.878 kb on + strand, within Psyr_4047 at 4830.878 kb on + strand, within Psyr_4047 at 4830.879 kb on - strand, within Psyr_4047 at 4830.879 kb on - strand, within Psyr_4047 at 4830.879 kb on - strand, within Psyr_4047 at 4830.887 kb on - strand, within Psyr_4047 at 4831.026 kb on + strand, within Psyr_4047 at 4831.026 kb on + strand, within Psyr_4047 at 4831.027 kb on - strand, within Psyr_4047 at 4831.032 kb on + strand, within Psyr_4047 at 4831.032 kb on + strand, within Psyr_4047 at 4831.032 kb on + strand, within Psyr_4047 at 4831.032 kb on + strand, within Psyr_4047 at 4831.032 kb on + strand, within Psyr_4047 at 4831.032 kb on + strand, within Psyr_4047 at 4831.032 kb on + strand, within Psyr_4047 at 4831.032 kb on + strand, within Psyr_4047 at 4831.032 kb on + strand, within Psyr_4047 at 4831.032 kb on + strand, within Psyr_4047 at 4831.033 kb on - strand, within Psyr_4047 at 4831.033 kb on - strand, within Psyr_4047 at 4831.033 kb on - strand, within Psyr_4047 at 4831.033 kb on - strand, within Psyr_4047 at 4831.033 kb on - strand, within Psyr_4047 at 4831.105 kb on - strand, within Psyr_4047 at 4831.105 kb on - strand, within Psyr_4047 at 4831.105 kb on - strand, within Psyr_4047 at 4831.105 kb on - strand, within Psyr_4047 at 4831.105 kb on - strand, within Psyr_4047 at 4831.105 kb on - strand, within Psyr_4047 at 4831.140 kb on + strand, within Psyr_4047 at 4831.140 kb on + strand, within Psyr_4047 at 4831.141 kb on - strand, within Psyr_4047 at 4831.141 kb on - strand, within Psyr_4047 at 4831.146 kb on + strand, within Psyr_4047 at 4831.227 kb on + strand, within Psyr_4047 at 4831.228 kb on - strand, within Psyr_4047 at 4831.228 kb on - strand, within Psyr_4047 at 4831.365 kb on + strand, within Psyr_4047 at 4831.365 kb on + strand, within Psyr_4047 at 4831.365 kb on + strand, within Psyr_4047 at 4831.365 kb on + strand, within Psyr_4047 at 4831.366 kb on - strand, within Psyr_4047 at 4831.366 kb on - strand, within Psyr_4047 at 4831.449 kb on + strand, within Psyr_4047
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 4,829,195 - Psyr_4044 0.75 -1.9 4,829,359 + Psyr_4044 0.89 -3.3 4,829,359 + Psyr_4044 0.89 +1.1 4,829,359 + Psyr_4044 0.89 -0.0 4,829,486 + +1.0 4,829,677 - Psyr_4045 0.32 -0.5 4,829,677 - Psyr_4045 0.32 -1.0 4,829,678 + Psyr_4045 0.33 +0.1 4,829,678 + Psyr_4045 0.33 +4.0 4,829,678 + Psyr_4045 0.33 +0.1 4,829,678 + Psyr_4045 0.33 +4.8 4,829,678 + Psyr_4045 0.33 +2.4 4,829,678 + Psyr_4045 0.33 +3.9 4,829,678 + Psyr_4045 0.33 +0.9 4,829,679 - Psyr_4045 0.33 -0.8 4,829,679 - Psyr_4045 0.33 +3.8 4,829,679 - Psyr_4045 0.33 -0.4 4,829,754 + Psyr_4045 0.46 -1.7 4,829,879 + Psyr_4045 0.67 -0.3 4,830,000 - Psyr_4045 0.88 -1.3 4,830,020 - +0.1 4,830,060 + +0.1 4,830,060 + -0.4 4,830,060 + -0.7 4,830,278 - Psyr_4046 0.36 -1.9 4,830,278 - Psyr_4046 0.36 -1.3 4,830,289 + Psyr_4046 0.39 +2.1 4,830,289 + Psyr_4046 0.39 -0.1 4,830,289 + Psyr_4046 0.39 +4.2 4,830,290 - Psyr_4046 0.40 +0.7 4,830,290 - Psyr_4046 0.40 -0.2 4,830,290 - Psyr_4046 0.40 +3.6 4,830,301 + Psyr_4046 0.43 -0.5 4,830,301 + Psyr_4046 0.43 -0.6 4,830,302 - Psyr_4046 0.43 -1.8 4,830,325 + Psyr_4046 0.50 +1.1 4,830,326 - Psyr_4046 0.51 +0.1 4,830,326 - Psyr_4046 0.51 -0.1 4,830,326 - Psyr_4046 0.51 +1.7 4,830,326 - Psyr_4046 0.51 +3.3 4,830,340 + Psyr_4046 0.55 +4.2 4,830,340 + Psyr_4046 0.55 -0.6 4,830,340 + Psyr_4046 0.55 +0.3 4,830,341 - Psyr_4046 0.55 +1.1 4,830,341 - Psyr_4046 0.55 -1.1 4,830,356 - Psyr_4046 0.60 -0.9 4,830,356 - Psyr_4046 0.60 +3.1 4,830,546 + +2.7 4,830,546 + +2.4 4,830,546 + +1.8 4,830,546 + +0.6 4,830,546 + +2.1 4,830,546 + -0.4 4,830,546 + -0.4 4,830,546 + -2.6 4,830,546 + +2.4 4,830,546 + -0.7 4,830,546 + -2.7 4,830,547 - -0.0 4,830,547 - -1.1 4,830,547 - -0.7 4,830,547 - +3.7 4,830,547 - +1.7 4,830,547 - +0.7 4,830,547 - -1.2 4,830,551 - -0.7 4,830,606 + Psyr_4047 0.11 +0.1 4,830,606 + Psyr_4047 0.11 -2.4 4,830,606 + Psyr_4047 0.11 -1.3 4,830,606 + Psyr_4047 0.11 -1.1 4,830,606 + Psyr_4047 0.11 -2.8 4,830,606 + Psyr_4047 0.11 +0.7 4,830,606 + Psyr_4047 0.11 -1.0 4,830,606 + Psyr_4047 0.11 +1.2 4,830,606 + Psyr_4047 0.11 +1.5 4,830,606 + Psyr_4047 0.11 +0.1 4,830,607 - Psyr_4047 0.11 -0.3 4,830,607 - Psyr_4047 0.11 +4.0 4,830,607 - Psyr_4047 0.11 +3.9 4,830,607 - Psyr_4047 0.11 +1.1 4,830,607 - Psyr_4047 0.11 -0.7 4,830,689 - Psyr_4047 0.18 -2.1 4,830,861 + Psyr_4047 0.34 +0.3 4,830,861 + Psyr_4047 0.34 -2.6 4,830,861 + Psyr_4047 0.34 +3.9 4,830,861 + Psyr_4047 0.34 +0.6 4,830,861 + Psyr_4047 0.34 +3.3 4,830,861 + Psyr_4047 0.34 +1.6 4,830,861 + Psyr_4047 0.34 -1.5 4,830,861 + Psyr_4047 0.34 +1.1 4,830,861 + Psyr_4047 0.34 -0.3 4,830,861 + Psyr_4047 0.34 -2.5 4,830,861 + Psyr_4047 0.34 -1.4 4,830,862 - Psyr_4047 0.34 +2.4 4,830,862 - Psyr_4047 0.34 -1.5 4,830,862 - Psyr_4047 0.34 -1.9 4,830,862 - Psyr_4047 0.34 -0.5 4,830,862 - Psyr_4047 0.34 +1.1 4,830,862 - Psyr_4047 0.34 -1.6 4,830,862 - Psyr_4047 0.34 -1.9 4,830,862 - Psyr_4047 0.34 -0.8 4,830,862 - Psyr_4047 0.34 +1.7 4,830,862 - Psyr_4047 0.34 +3.9 4,830,867 + Psyr_4047 0.35 -1.0 4,830,868 - Psyr_4047 0.35 -0.5 4,830,878 + Psyr_4047 0.36 +1.2 4,830,878 + Psyr_4047 0.36 -2.6 4,830,879 - Psyr_4047 0.36 -2.1 4,830,879 - Psyr_4047 0.36 -0.9 4,830,879 - Psyr_4047 0.36 -0.9 4,830,887 - Psyr_4047 0.36 -0.5 4,831,026 + Psyr_4047 0.49 -0.0 4,831,026 + Psyr_4047 0.49 +3.3 4,831,027 - Psyr_4047 0.49 -1.0 4,831,032 + Psyr_4047 0.49 +4.2 4,831,032 + Psyr_4047 0.49 +1.7 4,831,032 + Psyr_4047 0.49 -3.5 4,831,032 + Psyr_4047 0.49 -1.6 4,831,032 + Psyr_4047 0.49 +1.2 4,831,032 + Psyr_4047 0.49 -1.9 4,831,032 + Psyr_4047 0.49 +1.6 4,831,032 + Psyr_4047 0.49 +2.9 4,831,032 + Psyr_4047 0.49 -1.2 4,831,032 + Psyr_4047 0.49 +0.9 4,831,033 - Psyr_4047 0.50 +4.9 4,831,033 - Psyr_4047 0.50 -1.5 4,831,033 - Psyr_4047 0.50 -1.5 4,831,033 - Psyr_4047 0.50 -0.4 4,831,033 - Psyr_4047 0.50 +2.9 4,831,105 - Psyr_4047 0.56 -0.9 4,831,105 - Psyr_4047 0.56 -1.7 4,831,105 - Psyr_4047 0.56 +3.3 4,831,105 - Psyr_4047 0.56 +3.3 4,831,105 - Psyr_4047 0.56 -0.1 4,831,105 - Psyr_4047 0.56 -2.3 4,831,140 + Psyr_4047 0.59 +3.4 4,831,140 + Psyr_4047 0.59 +2.1 4,831,141 - Psyr_4047 0.59 +2.9 4,831,141 - Psyr_4047 0.59 +5.0 4,831,146 + Psyr_4047 0.60 +1.7 4,831,227 + Psyr_4047 0.67 -0.3 4,831,228 - Psyr_4047 0.67 -1.7 4,831,228 - Psyr_4047 0.67 +0.2 4,831,365 + Psyr_4047 0.79 +2.9 4,831,365 + Psyr_4047 0.79 +5.2 4,831,365 + Psyr_4047 0.79 +2.1 4,831,365 + Psyr_4047 0.79 -2.0 4,831,366 - Psyr_4047 0.80 -1.4 4,831,366 - Psyr_4047 0.80 -2.1 4,831,449 + Psyr_4047 0.87 -1.6
Or see this region's nucleotide sequence