Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4000 and Psyr_4001 are separated by 167 nucleotides Psyr_4001 and Psyr_4002 are separated by 145 nucleotides Psyr_4002 and Psyr_4003 are separated by 0 nucleotides Psyr_4003 and Psyr_4004 are separated by 462 nucleotides
Psyr_4000: Psyr_4000 - hypothetical protein, at 4,764,833 to 4,765,087
_4000
Psyr_4001: Psyr_4001 - conserved domain protein, at 4,765,255 to 4,765,572
_4001
Psyr_4002: Psyr_4002 - conserved hypothetical protein, at 4,765,718 to 4,766,341
_4002
Psyr_4003: Psyr_4003 - conserved hypothetical protein, at 4,766,342 to 4,766,581
_4003
Psyr_4004: Psyr_4004 - Drug resistance transporter EmrB/QacA subfamily, at 4,767,044 to 4,768,540
_4004
Position (kb) 4765
4766
4767 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 4764.766 kb on + strand at 4764.766 kb on + strand at 4764.767 kb on - strand at 4764.774 kb on + strand at 4764.775 kb on - strand at 4764.848 kb on + strand at 4764.848 kb on + strand at 4764.848 kb on + strand at 4764.848 kb on + strand at 4764.848 kb on + strand at 4764.848 kb on + strand at 4764.848 kb on + strand at 4764.848 kb on + strand at 4764.849 kb on - strand at 4764.849 kb on - strand at 4764.849 kb on - strand at 4764.849 kb on - strand at 4764.849 kb on - strand at 4764.849 kb on - strand at 4764.849 kb on - strand at 4764.849 kb on - strand at 4764.851 kb on - strand at 4764.896 kb on + strand, within Psyr_4000 at 4764.897 kb on - strand, within Psyr_4000 at 4764.908 kb on + strand, within Psyr_4000 at 4764.909 kb on - strand, within Psyr_4000 at 4764.909 kb on - strand, within Psyr_4000 at 4764.926 kb on + strand, within Psyr_4000 at 4764.926 kb on + strand, within Psyr_4000 at 4764.926 kb on + strand, within Psyr_4000 at 4764.926 kb on + strand, within Psyr_4000 at 4764.926 kb on + strand, within Psyr_4000 at 4764.927 kb on - strand, within Psyr_4000 at 4764.927 kb on - strand, within Psyr_4000 at 4764.927 kb on - strand, within Psyr_4000 at 4764.927 kb on - strand, within Psyr_4000 at 4764.927 kb on - strand, within Psyr_4000 at 4764.927 kb on - strand, within Psyr_4000 at 4764.953 kb on + strand, within Psyr_4000 at 4765.028 kb on - strand, within Psyr_4000 at 4765.090 kb on + strand at 4765.138 kb on + strand at 4765.139 kb on - strand at 4765.169 kb on + strand at 4765.169 kb on + strand at 4765.170 kb on - strand at 4765.172 kb on + strand at 4765.173 kb on - strand at 4765.173 kb on - strand at 4765.173 kb on - strand at 4765.181 kb on - strand at 4765.183 kb on + strand at 4765.183 kb on + strand at 4765.198 kb on + strand at 4765.198 kb on + strand at 4765.198 kb on + strand at 4765.213 kb on - strand at 4765.287 kb on + strand, within Psyr_4001 at 4765.288 kb on - strand, within Psyr_4001 at 4765.390 kb on - strand, within Psyr_4001 at 4765.550 kb on + strand at 4765.679 kb on + strand at 4765.679 kb on + strand at 4765.679 kb on + strand at 4765.680 kb on - strand at 4765.680 kb on - strand at 4765.694 kb on + strand at 4765.729 kb on + strand at 4765.730 kb on - strand at 4765.740 kb on - strand at 4765.791 kb on + strand, within Psyr_4002 at 4765.791 kb on + strand, within Psyr_4002 at 4765.856 kb on + strand, within Psyr_4002 at 4765.856 kb on + strand, within Psyr_4002 at 4765.856 kb on + strand, within Psyr_4002 at 4765.857 kb on - strand, within Psyr_4002 at 4765.857 kb on - strand, within Psyr_4002 at 4765.857 kb on - strand, within Psyr_4002 at 4765.880 kb on + strand, within Psyr_4002 at 4765.880 kb on + strand, within Psyr_4002 at 4765.880 kb on + strand, within Psyr_4002 at 4765.880 kb on + strand, within Psyr_4002 at 4765.880 kb on + strand, within Psyr_4002 at 4765.881 kb on - strand, within Psyr_4002 at 4765.881 kb on - strand, within Psyr_4002 at 4765.881 kb on - strand, within Psyr_4002 at 4765.933 kb on + strand, within Psyr_4002 at 4765.933 kb on + strand, within Psyr_4002 at 4765.933 kb on + strand, within Psyr_4002 at 4765.933 kb on + strand, within Psyr_4002 at 4765.937 kb on - strand, within Psyr_4002 at 4766.092 kb on - strand, within Psyr_4002 at 4766.238 kb on - strand, within Psyr_4002 at 4766.238 kb on - strand, within Psyr_4002 at 4766.288 kb on + strand at 4766.288 kb on + strand at 4766.515 kb on + strand, within Psyr_4003 at 4766.515 kb on + strand, within Psyr_4003 at 4766.515 kb on + strand, within Psyr_4003 at 4766.517 kb on + strand, within Psyr_4003 at 4766.517 kb on + strand, within Psyr_4003 at 4766.517 kb on + strand, within Psyr_4003 at 4766.517 kb on + strand, within Psyr_4003 at 4766.517 kb on + strand, within Psyr_4003 at 4766.517 kb on + strand, within Psyr_4003 at 4766.517 kb on + strand, within Psyr_4003 at 4766.518 kb on - strand, within Psyr_4003 at 4766.565 kb on + strand at 4766.565 kb on + strand at 4766.565 kb on + strand at 4766.565 kb on + strand at 4766.566 kb on - strand at 4766.566 kb on - strand at 4766.566 kb on - strand at 4766.566 kb on - strand at 4766.566 kb on - strand at 4766.600 kb on + strand at 4766.600 kb on + strand at 4766.620 kb on - strand at 4766.693 kb on - strand at 4766.693 kb on - strand at 4766.693 kb on - strand at 4766.699 kb on + strand at 4766.699 kb on + strand at 4766.700 kb on - strand at 4766.733 kb on + strand at 4766.733 kb on + strand at 4766.734 kb on - strand at 4766.757 kb on - strand at 4766.804 kb on + strand at 4766.805 kb on - strand at 4766.805 kb on - strand at 4766.805 kb on - strand at 4766.807 kb on - strand at 4766.807 kb on - strand at 4766.822 kb on - strand at 4766.851 kb on + strand at 4766.851 kb on + strand at 4766.852 kb on - strand at 4766.852 kb on - strand at 4766.865 kb on + strand at 4766.865 kb on + strand at 4766.865 kb on + strand at 4766.865 kb on + strand at 4766.866 kb on - strand at 4766.866 kb on - strand at 4766.866 kb on - strand at 4766.968 kb on - strand at 4766.984 kb on - strand at 4767.055 kb on + strand at 4767.186 kb on + strand at 4767.187 kb on - strand at 4767.187 kb on - strand at 4767.209 kb on + strand, within Psyr_4004 at 4767.209 kb on + strand, within Psyr_4004 at 4767.209 kb on + strand, within Psyr_4004 at 4767.209 kb on + strand, within Psyr_4004 at 4767.210 kb on - strand, within Psyr_4004 at 4767.210 kb on - strand, within Psyr_4004 at 4767.210 kb on - strand, within Psyr_4004 at 4767.210 kb on - strand, within Psyr_4004
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 4,764,766 + -1.2 4,764,766 + +3.9 4,764,767 - +3.1 4,764,774 + +0.4 4,764,775 - -1.7 4,764,848 + -1.6 4,764,848 + -2.5 4,764,848 + +0.5 4,764,848 + -3.0 4,764,848 + -0.3 4,764,848 + -0.2 4,764,848 + -2.7 4,764,848 + -1.7 4,764,849 - +2.1 4,764,849 - -2.5 4,764,849 - +3.4 4,764,849 - -1.1 4,764,849 - -0.2 4,764,849 - -0.7 4,764,849 - +3.1 4,764,849 - +2.7 4,764,851 - +3.6 4,764,896 + Psyr_4000 0.25 -2.4 4,764,897 - Psyr_4000 0.25 +0.5 4,764,908 + Psyr_4000 0.29 +2.4 4,764,909 - Psyr_4000 0.30 -0.7 4,764,909 - Psyr_4000 0.30 +3.7 4,764,926 + Psyr_4000 0.36 +0.1 4,764,926 + Psyr_4000 0.36 +3.1 4,764,926 + Psyr_4000 0.36 +2.9 4,764,926 + Psyr_4000 0.36 -0.1 4,764,926 + Psyr_4000 0.36 -1.7 4,764,927 - Psyr_4000 0.37 -4.0 4,764,927 - Psyr_4000 0.37 +1.1 4,764,927 - Psyr_4000 0.37 +0.5 4,764,927 - Psyr_4000 0.37 -1.9 4,764,927 - Psyr_4000 0.37 +3.6 4,764,927 - Psyr_4000 0.37 -0.8 4,764,953 + Psyr_4000 0.47 -3.2 4,765,028 - Psyr_4000 0.76 -1.7 4,765,090 + +1.1 4,765,138 + +2.9 4,765,139 - +1.1 4,765,169 + -0.9 4,765,169 + +0.1 4,765,170 - -0.3 4,765,172 + -0.5 4,765,173 - +1.1 4,765,173 - +2.4 4,765,173 - +4.7 4,765,181 - +3.6 4,765,183 + -1.0 4,765,183 + +3.8 4,765,198 + -1.9 4,765,198 + +3.9 4,765,198 + +2.9 4,765,213 - -1.1 4,765,287 + Psyr_4001 0.10 +2.7 4,765,288 - Psyr_4001 0.10 +5.0 4,765,390 - Psyr_4001 0.42 -4.5 4,765,550 + +3.1 4,765,679 + +0.1 4,765,679 + +2.4 4,765,679 + +0.1 4,765,680 - -1.4 4,765,680 - +3.8 4,765,694 + -1.1 4,765,729 + +0.4 4,765,730 - +0.6 4,765,740 - +1.1 4,765,791 + Psyr_4002 0.12 +4.5 4,765,791 + Psyr_4002 0.12 -1.5 4,765,856 + Psyr_4002 0.22 +2.1 4,765,856 + Psyr_4002 0.22 -1.9 4,765,856 + Psyr_4002 0.22 +3.1 4,765,857 - Psyr_4002 0.22 +3.3 4,765,857 - Psyr_4002 0.22 +1.7 4,765,857 - Psyr_4002 0.22 +3.4 4,765,880 + Psyr_4002 0.26 -0.4 4,765,880 + Psyr_4002 0.26 +2.1 4,765,880 + Psyr_4002 0.26 -0.7 4,765,880 + Psyr_4002 0.26 +2.9 4,765,880 + Psyr_4002 0.26 +3.1 4,765,881 - Psyr_4002 0.26 -0.2 4,765,881 - Psyr_4002 0.26 +1.7 4,765,881 - Psyr_4002 0.26 -0.2 4,765,933 + Psyr_4002 0.34 +4.2 4,765,933 + Psyr_4002 0.34 +3.6 4,765,933 + Psyr_4002 0.34 +0.1 4,765,933 + Psyr_4002 0.34 +4.4 4,765,937 - Psyr_4002 0.35 +0.5 4,766,092 - Psyr_4002 0.60 +1.7 4,766,238 - Psyr_4002 0.83 +0.1 4,766,238 - Psyr_4002 0.83 -0.8 4,766,288 + -0.1 4,766,288 + +3.3 4,766,515 + Psyr_4003 0.72 +1.1 4,766,515 + Psyr_4003 0.72 +1.7 4,766,515 + Psyr_4003 0.72 -2.3 4,766,517 + Psyr_4003 0.73 -2.7 4,766,517 + Psyr_4003 0.73 -1.0 4,766,517 + Psyr_4003 0.73 +1.7 4,766,517 + Psyr_4003 0.73 +0.1 4,766,517 + Psyr_4003 0.73 +1.1 4,766,517 + Psyr_4003 0.73 +0.1 4,766,517 + Psyr_4003 0.73 -1.0 4,766,518 - Psyr_4003 0.73 -2.4 4,766,565 + +0.5 4,766,565 + +0.5 4,766,565 + +0.2 4,766,565 + +3.6 4,766,566 - -1.0 4,766,566 - -0.3 4,766,566 - -0.7 4,766,566 - -1.5 4,766,566 - +3.4 4,766,600 + -0.6 4,766,600 + -0.4 4,766,620 - +2.7 4,766,693 - +3.3 4,766,693 - +2.1 4,766,693 - +2.7 4,766,699 + -0.7 4,766,699 + -1.3 4,766,700 - -1.7 4,766,733 + +0.7 4,766,733 + +0.1 4,766,734 - -1.7 4,766,757 - +0.1 4,766,804 + -0.5 4,766,805 - -2.0 4,766,805 - +0.2 4,766,805 - +2.1 4,766,807 - +1.6 4,766,807 - -3.2 4,766,822 - -2.1 4,766,851 + +3.6 4,766,851 + +0.3 4,766,852 - +4.2 4,766,852 - -0.5 4,766,865 + +0.2 4,766,865 + +1.7 4,766,865 + +1.7 4,766,865 + -0.8 4,766,866 - -2.2 4,766,866 - +0.4 4,766,866 - +1.3 4,766,968 - +3.8 4,766,984 - -1.1 4,767,055 + +1.1 4,767,186 + -2.1 4,767,187 - +1.1 4,767,187 - -2.1 4,767,209 + Psyr_4004 0.11 -1.1 4,767,209 + Psyr_4004 0.11 +1.1 4,767,209 + Psyr_4004 0.11 -0.7 4,767,209 + Psyr_4004 0.11 +0.8 4,767,210 - Psyr_4004 0.11 -0.6 4,767,210 - Psyr_4004 0.11 +2.7 4,767,210 - Psyr_4004 0.11 -0.6 4,767,210 - Psyr_4004 0.11 +0.6
Or see this region's nucleotide sequence