Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_3954

Experiment: KB with Acridine orange 62.5 ug/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_3952 and Psyr_3953 are separated by 114 nucleotidesPsyr_3953 and Psyr_3954 are separated by 9 nucleotidesPsyr_3954 and Psyr_3955 are separated by 171 nucleotides Psyr_3952: Psyr_3952 - conserved hypothetical protein, at 4,699,372 to 4,699,749 _3952 Psyr_3953: Psyr_3953 - signal peptidase I, Serine peptidase, MEROPS family S26A, at 4,699,864 to 4,700,718 _3953 Psyr_3954: Psyr_3954 - GTP-binding protein LepA, at 4,700,728 to 4,702,524 _3954 Psyr_3955: Psyr_3955 - Peptidase S1, chymotrypsin:PDZ/DHR/GLGF, at 4,702,696 to 4,704,141 _3955 Position (kb) 4700 4701 4702 4703Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 4699.738 kb on - strandat 4699.738 kb on - strandat 4699.738 kb on - strandat 4699.738 kb on - strandat 4699.738 kb on - strandat 4700.719 kb on - strandat 4700.830 kb on - strandat 4700.830 kb on - strandat 4700.870 kb on - strandat 4701.000 kb on - strand, within Psyr_3954at 4701.000 kb on - strand, within Psyr_3954at 4701.000 kb on - strand, within Psyr_3954at 4701.000 kb on - strand, within Psyr_3954at 4701.000 kb on - strand, within Psyr_3954at 4701.123 kb on - strand, within Psyr_3954at 4701.259 kb on - strand, within Psyr_3954at 4701.259 kb on - strand, within Psyr_3954at 4701.259 kb on - strand, within Psyr_3954at 4701.259 kb on - strand, within Psyr_3954at 4701.259 kb on - strand, within Psyr_3954at 4701.369 kb on - strand, within Psyr_3954at 4701.441 kb on - strand, within Psyr_3954at 4701.441 kb on - strand, within Psyr_3954at 4701.441 kb on - strand, within Psyr_3954at 4701.441 kb on - strand, within Psyr_3954at 4701.909 kb on - strand, within Psyr_3954at 4702.167 kb on - strand, within Psyr_3954at 4702.260 kb on - strand, within Psyr_3954at 4702.329 kb on - strand, within Psyr_3954at 4702.332 kb on - strand, within Psyr_3954at 4702.584 kb on - strandat 4702.593 kb on - strandat 4702.593 kb on - strandat 4702.612 kb on + strandat 4702.613 kb on - strandat 4702.710 kb on + strandat 4702.710 kb on + strandat 4703.516 kb on + strand, within Psyr_3955at 4703.516 kb on + strand, within Psyr_3955at 4703.516 kb on + strand, within Psyr_3955at 4703.516 kb on + strand, within Psyr_3955at 4703.516 kb on + strand, within Psyr_3955at 4703.516 kb on + strand, within Psyr_3955at 4703.516 kb on + strand, within Psyr_3955at 4703.517 kb on - strand, within Psyr_3955at 4703.517 kb on - strand, within Psyr_3955at 4703.517 kb on - strand, within Psyr_3955at 4703.517 kb on - strand, within Psyr_3955at 4703.517 kb on - strand, within Psyr_3955at 4703.517 kb on - strand, within Psyr_3955at 4703.517 kb on - strand, within Psyr_3955at 4703.517 kb on - strand, within Psyr_3955

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml
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4,699,738 - +0.2
4,699,738 - +3.0
4,699,738 - -4.3
4,699,738 - +3.2
4,699,738 - -0.7
4,700,719 - +0.0
4,700,830 - +0.0
4,700,830 - -2.6
4,700,870 - +1.6
4,701,000 - Psyr_3954 0.15 -0.8
4,701,000 - Psyr_3954 0.15 -3.7
4,701,000 - Psyr_3954 0.15 +1.6
4,701,000 - Psyr_3954 0.15 +2.4
4,701,000 - Psyr_3954 0.15 +1.0
4,701,123 - Psyr_3954 0.22 +2.0
4,701,259 - Psyr_3954 0.30 +1.0
4,701,259 - Psyr_3954 0.30 -2.3
4,701,259 - Psyr_3954 0.30 -4.5
4,701,259 - Psyr_3954 0.30 -1.0
4,701,259 - Psyr_3954 0.30 -2.0
4,701,369 - Psyr_3954 0.36 +1.0
4,701,441 - Psyr_3954 0.40 -1.7
4,701,441 - Psyr_3954 0.40 -4.3
4,701,441 - Psyr_3954 0.40 -4.6
4,701,441 - Psyr_3954 0.40 +1.6
4,701,909 - Psyr_3954 0.66 +0.0
4,702,167 - Psyr_3954 0.80 -1.1
4,702,260 - Psyr_3954 0.85 -3.2
4,702,329 - Psyr_3954 0.89 -4.9
4,702,332 - Psyr_3954 0.89 -3.6
4,702,584 - -2.1
4,702,593 - +2.8
4,702,593 - -1.4
4,702,612 + -1.7
4,702,613 - +3.7
4,702,710 + +1.1
4,702,710 + +2.8
4,703,516 + Psyr_3955 0.57 +1.0
4,703,516 + Psyr_3955 0.57 +0.0
4,703,516 + Psyr_3955 0.57 +2.4
4,703,516 + Psyr_3955 0.57 +2.4
4,703,516 + Psyr_3955 0.57 +1.0
4,703,516 + Psyr_3955 0.57 -1.3
4,703,516 + Psyr_3955 0.57 -1.6
4,703,517 - Psyr_3955 0.57 -0.7
4,703,517 - Psyr_3955 0.57 -1.0
4,703,517 - Psyr_3955 0.57 +2.0
4,703,517 - Psyr_3955 0.57 -0.3
4,703,517 - Psyr_3955 0.57 -2.0
4,703,517 - Psyr_3955 0.57 +1.0
4,703,517 - Psyr_3955 0.57 +2.8
4,703,517 - Psyr_3955 0.57 +0.0

Or see this region's nucleotide sequence