Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3836 and Psyr_3837 are separated by 39 nucleotides Psyr_3837 and Psyr_3838 are separated by 185 nucleotides Psyr_3838 and Psyr_3839 are separated by 171 nucleotides
Psyr_3836: Psyr_3836 - AMP-dependent synthetase and ligase, at 4,571,883 to 4,573,571
_3836
Psyr_3837: Psyr_3837 - conserved hypothetical protein, at 4,573,611 to 4,573,754
_3837
Psyr_3838: Psyr_3838 - MaoC-like dehydratase, at 4,573,940 to 4,574,410
_3838
Psyr_3839: Psyr_3839 - type III helper protein HopAK1, at 4,574,582 to 4,576,213
_3839
Position (kb) 4573
4574 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 4572.760 kb on + strand, within Psyr_3836 at 4572.761 kb on - strand, within Psyr_3836 at 4572.770 kb on - strand, within Psyr_3836 at 4572.853 kb on + strand, within Psyr_3836 at 4572.931 kb on + strand, within Psyr_3836 at 4572.932 kb on - strand, within Psyr_3836 at 4573.073 kb on + strand, within Psyr_3836 at 4573.073 kb on + strand, within Psyr_3836 at 4573.074 kb on - strand, within Psyr_3836 at 4573.133 kb on - strand, within Psyr_3836 at 4573.247 kb on - strand, within Psyr_3836 at 4573.247 kb on - strand, within Psyr_3836 at 4573.296 kb on + strand, within Psyr_3836 at 4573.417 kb on + strand at 4573.505 kb on - strand at 4573.505 kb on - strand at 4573.572 kb on + strand at 4573.573 kb on - strand at 4573.595 kb on + strand at 4573.595 kb on + strand at 4573.595 kb on + strand at 4573.595 kb on + strand at 4573.595 kb on + strand at 4573.595 kb on + strand at 4573.596 kb on - strand at 4573.596 kb on - strand at 4573.596 kb on - strand at 4573.596 kb on - strand at 4573.596 kb on - strand at 4573.596 kb on - strand at 4573.596 kb on - strand at 4573.596 kb on - strand at 4573.619 kb on + strand at 4573.620 kb on - strand at 4573.620 kb on - strand at 4573.637 kb on + strand, within Psyr_3837 at 4573.637 kb on + strand, within Psyr_3837 at 4573.638 kb on - strand, within Psyr_3837 at 4573.705 kb on - strand, within Psyr_3837 at 4573.755 kb on + strand at 4573.755 kb on + strand at 4573.755 kb on + strand at 4573.755 kb on + strand at 4573.756 kb on - strand at 4573.756 kb on - strand at 4573.756 kb on - strand at 4573.761 kb on + strand at 4573.762 kb on - strand at 4573.762 kb on - strand at 4573.762 kb on - strand at 4573.762 kb on - strand at 4573.780 kb on + strand at 4573.780 kb on + strand at 4573.783 kb on + strand at 4573.783 kb on + strand at 4573.783 kb on + strand at 4573.784 kb on - strand at 4573.784 kb on - strand at 4573.784 kb on - strand at 4574.588 kb on - strand at 4574.603 kb on + strand at 4574.603 kb on + strand at 4574.603 kb on + strand at 4574.603 kb on + strand at 4574.603 kb on + strand at 4574.603 kb on + strand at 4574.603 kb on + strand at 4574.603 kb on + strand at 4574.603 kb on + strand at 4574.603 kb on + strand at 4574.604 kb on - strand at 4574.604 kb on - strand at 4574.604 kb on - strand at 4574.604 kb on - strand at 4574.695 kb on + strand at 4574.696 kb on - strand at 4574.696 kb on - strand at 4574.696 kb on - strand at 4574.696 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 4,572,760 + Psyr_3836 0.52 -1.4 4,572,761 - Psyr_3836 0.52 +3.3 4,572,770 - Psyr_3836 0.53 -0.1 4,572,853 + Psyr_3836 0.57 -0.0 4,572,931 + Psyr_3836 0.62 +4.6 4,572,932 - Psyr_3836 0.62 -1.2 4,573,073 + Psyr_3836 0.70 +3.3 4,573,073 + Psyr_3836 0.70 +0.3 4,573,074 - Psyr_3836 0.71 +0.3 4,573,133 - Psyr_3836 0.74 -3.3 4,573,247 - Psyr_3836 0.81 +2.0 4,573,247 - Psyr_3836 0.81 -2.0 4,573,296 + Psyr_3836 0.84 -1.7 4,573,417 + -1.9 4,573,505 - +0.2 4,573,505 - +2.3 4,573,572 + -0.6 4,573,573 - +2.0 4,573,595 + -1.8 4,573,595 + +2.0 4,573,595 + -1.9 4,573,595 + -0.3 4,573,595 + +1.6 4,573,595 + +3.1 4,573,596 - +1.6 4,573,596 - -2.1 4,573,596 - +4.7 4,573,596 - -0.1 4,573,596 - +0.2 4,573,596 - -1.0 4,573,596 - +1.6 4,573,596 - +0.7 4,573,619 + -2.3 4,573,620 - -1.0 4,573,620 - -0.0 4,573,637 + Psyr_3837 0.18 -3.0 4,573,637 + Psyr_3837 0.18 +0.2 4,573,638 - Psyr_3837 0.19 +1.0 4,573,705 - Psyr_3837 0.65 -2.5 4,573,755 + +2.0 4,573,755 + +1.0 4,573,755 + -0.0 4,573,755 + -0.0 4,573,756 - +3.9 4,573,756 - +0.4 4,573,756 - +0.3 4,573,761 + -1.8 4,573,762 - +3.4 4,573,762 - -0.9 4,573,762 - +2.3 4,573,762 - +4.3 4,573,780 + +1.6 4,573,780 + +3.7 4,573,783 + -4.1 4,573,783 + -1.9 4,573,783 + +2.0 4,573,784 - +1.0 4,573,784 - -1.4 4,573,784 - +1.6 4,574,588 - -1.4 4,574,603 + -1.9 4,574,603 + +2.8 4,574,603 + +1.0 4,574,603 + -1.7 4,574,603 + -2.6 4,574,603 + -0.8 4,574,603 + -2.2 4,574,603 + -2.6 4,574,603 + -0.0 4,574,603 + +1.0 4,574,604 - -1.1 4,574,604 - +3.6 4,574,604 - +3.6 4,574,604 - +3.4 4,574,695 + -0.0 4,574,696 - -1.7 4,574,696 - -0.6 4,574,696 - -1.7 4,574,696 - -2.0
Or see this region's nucleotide sequence