Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3807 and Psyr_3808 overlap by 4 nucleotides Psyr_3808 and Psyr_3809 overlap by 4 nucleotides
Psyr_3807: Psyr_3807 - hypothetical protein, at 4,527,902 to 4,529,116
_3807
Psyr_3808: Psyr_3808 - hypothetical protein, at 4,529,113 to 4,529,592
_3808
Psyr_3809: Psyr_3809 - hypothetical protein, at 4,529,589 to 4,531,439
_3809
Position (kb) 4527
4528
4529
4530 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 4526.988 kb on + strand at 4527.025 kb on + strand at 4527.025 kb on + strand at 4527.025 kb on + strand at 4527.026 kb on - strand at 4527.029 kb on + strand at 4527.030 kb on - strand at 4527.030 kb on - strand at 4527.030 kb on - strand at 4527.103 kb on - strand at 4527.103 kb on - strand at 4527.134 kb on - strand at 4527.218 kb on + strand at 4527.330 kb on + strand at 4527.330 kb on + strand at 4527.330 kb on + strand at 4527.330 kb on + strand at 4527.330 kb on + strand at 4527.330 kb on + strand at 4527.331 kb on - strand at 4527.331 kb on - strand at 4527.331 kb on - strand at 4527.331 kb on - strand at 4527.331 kb on - strand at 4527.381 kb on + strand at 4527.381 kb on + strand at 4527.382 kb on - strand at 4527.447 kb on - strand at 4527.527 kb on - strand at 4527.545 kb on - strand at 4527.545 kb on - strand at 4527.545 kb on - strand at 4527.623 kb on + strand at 4527.623 kb on + strand at 4527.644 kb on + strand at 4527.644 kb on + strand at 4527.645 kb on - strand at 4527.645 kb on - strand at 4527.645 kb on - strand at 4527.648 kb on - strand at 4527.749 kb on + strand at 4527.750 kb on - strand at 4527.764 kb on - strand at 4527.764 kb on - strand at 4527.818 kb on + strand at 4527.818 kb on + strand at 4527.818 kb on + strand at 4527.829 kb on - strand at 4527.975 kb on - strand at 4527.977 kb on + strand at 4528.093 kb on + strand, within Psyr_3807 at 4528.093 kb on + strand, within Psyr_3807 at 4528.131 kb on + strand, within Psyr_3807 at 4528.132 kb on - strand, within Psyr_3807 at 4528.141 kb on - strand, within Psyr_3807 at 4528.141 kb on - strand, within Psyr_3807 at 4528.344 kb on + strand, within Psyr_3807 at 4528.348 kb on - strand, within Psyr_3807 at 4528.410 kb on + strand, within Psyr_3807 at 4528.411 kb on - strand, within Psyr_3807 at 4528.411 kb on - strand, within Psyr_3807 at 4528.411 kb on - strand, within Psyr_3807 at 4528.489 kb on + strand, within Psyr_3807 at 4528.567 kb on - strand, within Psyr_3807 at 4528.600 kb on + strand, within Psyr_3807 at 4528.600 kb on + strand, within Psyr_3807 at 4528.703 kb on + strand, within Psyr_3807 at 4528.703 kb on + strand, within Psyr_3807 at 4528.704 kb on - strand, within Psyr_3807 at 4528.704 kb on - strand, within Psyr_3807 at 4528.704 kb on - strand, within Psyr_3807 at 4528.729 kb on - strand, within Psyr_3807 at 4528.817 kb on - strand, within Psyr_3807 at 4528.993 kb on + strand, within Psyr_3807 at 4529.036 kb on - strand at 4529.081 kb on - strand at 4529.095 kb on + strand at 4529.148 kb on - strand at 4529.333 kb on + strand, within Psyr_3808 at 4529.509 kb on - strand, within Psyr_3808 at 4529.582 kb on + strand at 4529.611 kb on + strand at 4529.612 kb on - strand at 4529.645 kb on + strand at 4529.675 kb on - strand at 4529.678 kb on - strand at 4529.767 kb on + strand at 4529.809 kb on + strand, within Psyr_3809 at 4529.809 kb on + strand, within Psyr_3809 at 4529.888 kb on - strand, within Psyr_3809 at 4529.888 kb on - strand, within Psyr_3809 at 4529.934 kb on - strand, within Psyr_3809 at 4530.084 kb on + strand, within Psyr_3809
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 4,526,988 + -1.2 4,527,025 + +1.5 4,527,025 + -3.6 4,527,025 + +1.6 4,527,026 - +1.5 4,527,029 + +4.3 4,527,030 - -0.1 4,527,030 - +3.1 4,527,030 - -1.9 4,527,103 - -4.8 4,527,103 - -4.4 4,527,134 - -5.3 4,527,218 + -0.4 4,527,330 + +2.5 4,527,330 + -0.8 4,527,330 + -3.1 4,527,330 + +1.5 4,527,330 + -1.4 4,527,330 + -0.8 4,527,331 - -0.4 4,527,331 - -3.0 4,527,331 - -2.0 4,527,331 - +2.5 4,527,331 - -4.3 4,527,381 + -1.7 4,527,381 + +0.9 4,527,382 - -0.1 4,527,447 - +3.1 4,527,527 - -0.1 4,527,545 - -2.0 4,527,545 - +1.9 4,527,545 - +0.9 4,527,623 + -0.1 4,527,623 + -1.2 4,527,644 + -1.8 4,527,644 + -1.1 4,527,645 - -0.1 4,527,645 - +1.5 4,527,645 - +2.5 4,527,648 - -0.8 4,527,749 + +1.5 4,527,750 - +2.5 4,527,764 - +3.1 4,527,764 - -3.2 4,527,818 + +2.5 4,527,818 + -0.7 4,527,818 + +3.1 4,527,829 - -1.1 4,527,975 - +0.9 4,527,977 + -3.1 4,528,093 + Psyr_3807 0.16 -2.4 4,528,093 + Psyr_3807 0.16 +0.9 4,528,131 + Psyr_3807 0.19 -0.1 4,528,132 - Psyr_3807 0.19 +0.9 4,528,141 - Psyr_3807 0.20 -2.9 4,528,141 - Psyr_3807 0.20 +2.5 4,528,344 + Psyr_3807 0.36 -1.5 4,528,348 - Psyr_3807 0.37 +1.9 4,528,410 + Psyr_3807 0.42 -3.4 4,528,411 - Psyr_3807 0.42 -1.3 4,528,411 - Psyr_3807 0.42 +0.9 4,528,411 - Psyr_3807 0.42 -2.4 4,528,489 + Psyr_3807 0.48 -4.0 4,528,567 - Psyr_3807 0.55 +1.5 4,528,600 + Psyr_3807 0.57 -2.8 4,528,600 + Psyr_3807 0.57 -0.8 4,528,703 + Psyr_3807 0.66 +2.8 4,528,703 + Psyr_3807 0.66 -1.9 4,528,704 - Psyr_3807 0.66 -2.3 4,528,704 - Psyr_3807 0.66 -4.1 4,528,704 - Psyr_3807 0.66 -2.8 4,528,729 - Psyr_3807 0.68 -0.1 4,528,817 - Psyr_3807 0.75 -1.6 4,528,993 + Psyr_3807 0.90 +2.3 4,529,036 - -0.1 4,529,081 - -2.6 4,529,095 + -0.1 4,529,148 - -3.8 4,529,333 + Psyr_3808 0.46 +2.9 4,529,509 - Psyr_3808 0.82 -3.4 4,529,582 + -2.9 4,529,611 + -3.1 4,529,612 - -1.6 4,529,645 + -0.1 4,529,675 - +1.5 4,529,678 - -0.1 4,529,767 + +1.9 4,529,809 + Psyr_3809 0.12 -0.5 4,529,809 + Psyr_3809 0.12 -0.1 4,529,888 - Psyr_3809 0.16 -3.7 4,529,888 - Psyr_3809 0.16 +2.3 4,529,934 - Psyr_3809 0.19 +1.9 4,530,084 + Psyr_3809 0.27 -1.1
Or see this region's nucleotide sequence