Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3717 and Psyr_3718 are separated by 132 nucleotides Psyr_3718 and Psyr_3719 are separated by 113 nucleotides Psyr_3719 and Psyr_3720 are separated by 322 nucleotides
Psyr_3717: Psyr_3717 - Ribonucleoside-diphosphate reductase, at 4,404,442 to 4,407,354
_3717
Psyr_3718: Psyr_3718 - hypothetical protein, at 4,407,487 to 4,407,840
_3718
Psyr_3719: Psyr_3719 - hypothetical protein, at 4,407,954 to 4,408,313
_3719
Psyr_3720: Psyr_3720 - Filamentation induced by cAMP protein Fic, at 4,408,636 to 4,409,241
_3720
Position (kb) 4407
4408
4409 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 4407.353 kb on - strand at 4407.361 kb on + strand at 4407.362 kb on - strand at 4407.440 kb on + strand at 4407.441 kb on - strand at 4407.508 kb on - strand at 4407.508 kb on - strand at 4407.515 kb on + strand at 4407.516 kb on - strand at 4407.516 kb on - strand at 4407.517 kb on + strand at 4407.517 kb on + strand at 4407.517 kb on + strand at 4407.517 kb on + strand at 4407.518 kb on - strand at 4407.518 kb on - strand at 4407.518 kb on - strand at 4407.518 kb on - strand at 4407.535 kb on + strand, within Psyr_3718 at 4407.535 kb on + strand, within Psyr_3718 at 4407.535 kb on + strand, within Psyr_3718 at 4407.542 kb on + strand, within Psyr_3718 at 4407.543 kb on - strand, within Psyr_3718 at 4407.606 kb on - strand, within Psyr_3718 at 4407.672 kb on + strand, within Psyr_3718 at 4407.673 kb on - strand, within Psyr_3718 at 4407.707 kb on + strand, within Psyr_3718 at 4407.707 kb on + strand, within Psyr_3718 at 4407.707 kb on + strand, within Psyr_3718 at 4407.707 kb on + strand, within Psyr_3718 at 4407.708 kb on - strand, within Psyr_3718 at 4407.708 kb on - strand, within Psyr_3718 at 4407.708 kb on - strand, within Psyr_3718 at 4407.816 kb on + strand at 4407.816 kb on + strand at 4407.817 kb on - strand at 4407.817 kb on - strand at 4407.817 kb on - strand at 4407.822 kb on + strand at 4407.823 kb on - strand at 4407.831 kb on + strand at 4407.831 kb on + strand at 4407.832 kb on - strand at 4407.832 kb on - strand at 4407.856 kb on + strand at 4407.856 kb on + strand at 4407.856 kb on + strand at 4407.856 kb on + strand at 4407.856 kb on + strand at 4407.856 kb on + strand at 4407.856 kb on + strand at 4407.856 kb on + strand at 4407.856 kb on + strand at 4407.856 kb on + strand at 4407.856 kb on + strand at 4407.856 kb on + strand at 4407.857 kb on - strand at 4407.857 kb on - strand at 4407.857 kb on - strand at 4407.857 kb on - strand at 4407.857 kb on - strand at 4407.859 kb on + strand at 4407.859 kb on + strand at 4407.860 kb on - strand at 4407.860 kb on - strand at 4407.860 kb on - strand at 4407.871 kb on - strand at 4407.897 kb on + strand at 4407.944 kb on - strand at 4407.974 kb on + strand at 4407.974 kb on + strand at 4407.974 kb on + strand at 4407.974 kb on + strand at 4407.975 kb on - strand at 4408.079 kb on + strand, within Psyr_3719 at 4408.079 kb on + strand, within Psyr_3719 at 4408.080 kb on - strand, within Psyr_3719 at 4408.080 kb on - strand, within Psyr_3719 at 4408.089 kb on - strand, within Psyr_3719 at 4408.089 kb on - strand, within Psyr_3719 at 4408.097 kb on + strand, within Psyr_3719 at 4408.142 kb on + strand, within Psyr_3719 at 4408.143 kb on - strand, within Psyr_3719 at 4408.160 kb on + strand, within Psyr_3719 at 4408.187 kb on - strand, within Psyr_3719 at 4408.187 kb on - strand, within Psyr_3719 at 4408.187 kb on - strand, within Psyr_3719 at 4408.219 kb on + strand, within Psyr_3719 at 4408.219 kb on + strand, within Psyr_3719 at 4408.219 kb on + strand, within Psyr_3719 at 4408.219 kb on + strand, within Psyr_3719 at 4408.219 kb on + strand, within Psyr_3719 at 4408.219 kb on + strand, within Psyr_3719 at 4408.219 kb on + strand, within Psyr_3719 at 4408.219 kb on + strand, within Psyr_3719 at 4408.220 kb on - strand, within Psyr_3719 at 4408.220 kb on - strand, within Psyr_3719 at 4408.220 kb on - strand, within Psyr_3719 at 4408.220 kb on - strand, within Psyr_3719 at 4408.263 kb on + strand, within Psyr_3719 at 4408.263 kb on + strand, within Psyr_3719 at 4408.263 kb on + strand, within Psyr_3719 at 4408.263 kb on + strand, within Psyr_3719 at 4408.263 kb on + strand, within Psyr_3719 at 4408.263 kb on + strand, within Psyr_3719 at 4408.263 kb on + strand, within Psyr_3719 at 4408.264 kb on - strand, within Psyr_3719 at 4408.264 kb on - strand, within Psyr_3719 at 4408.264 kb on - strand, within Psyr_3719 at 4408.264 kb on - strand, within Psyr_3719 at 4408.264 kb on - strand, within Psyr_3719 at 4408.264 kb on - strand, within Psyr_3719 at 4408.264 kb on - strand, within Psyr_3719 at 4408.264 kb on - strand, within Psyr_3719 at 4408.264 kb on - strand, within Psyr_3719 at 4408.264 kb on - strand, within Psyr_3719 at 4408.268 kb on - strand, within Psyr_3719 at 4408.268 kb on - strand, within Psyr_3719 at 4408.311 kb on + strand at 4408.312 kb on - strand at 4408.330 kb on - strand at 4408.331 kb on + strand at 4408.331 kb on + strand at 4408.331 kb on + strand at 4408.331 kb on + strand at 4408.331 kb on + strand at 4408.331 kb on + strand at 4408.331 kb on + strand at 4408.331 kb on + strand at 4408.331 kb on + strand at 4408.331 kb on + strand at 4408.331 kb on + strand at 4408.331 kb on + strand at 4408.331 kb on + strand at 4408.331 kb on + strand at 4408.331 kb on + strand at 4408.331 kb on + strand at 4408.331 kb on + strand at 4408.332 kb on - strand at 4408.332 kb on - strand at 4408.332 kb on - strand at 4408.332 kb on - strand at 4408.332 kb on - strand at 4408.332 kb on - strand at 4408.332 kb on - strand at 4408.332 kb on - strand at 4408.332 kb on - strand at 4408.332 kb on - strand at 4408.332 kb on - strand at 4408.332 kb on - strand at 4408.332 kb on - strand at 4408.332 kb on - strand at 4408.332 kb on - strand at 4408.332 kb on - strand at 4408.332 kb on - strand at 4408.385 kb on - strand at 4408.385 kb on - strand at 4408.431 kb on + strand at 4408.431 kb on + strand at 4408.431 kb on + strand at 4408.432 kb on - strand at 4408.526 kb on + strand at 4408.526 kb on + strand at 4408.648 kb on + strand at 4408.648 kb on + strand at 4408.648 kb on + strand at 4408.648 kb on + strand at 4408.648 kb on + strand at 4408.649 kb on - strand at 4408.689 kb on + strand at 4408.727 kb on + strand, within Psyr_3720 at 4408.727 kb on + strand, within Psyr_3720 at 4408.728 kb on - strand, within Psyr_3720 at 4408.728 kb on - strand, within Psyr_3720 at 4408.728 kb on - strand, within Psyr_3720 at 4408.807 kb on + strand, within Psyr_3720 at 4408.808 kb on - strand, within Psyr_3720 at 4408.816 kb on + strand, within Psyr_3720 at 4408.816 kb on + strand at 4408.816 kb on + strand, within Psyr_3720 at 4408.817 kb on - strand, within Psyr_3720 at 4408.817 kb on - strand, within Psyr_3720 at 4408.846 kb on + strand, within Psyr_3720 at 4408.847 kb on - strand, within Psyr_3720 at 4408.865 kb on + strand, within Psyr_3720 at 4408.866 kb on - strand, within Psyr_3720 at 4408.874 kb on + strand, within Psyr_3720 at 4408.875 kb on - strand, within Psyr_3720 at 4408.875 kb on - strand, within Psyr_3720 at 4408.875 kb on - strand, within Psyr_3720 at 4408.875 kb on - strand, within Psyr_3720 at 4408.875 kb on - strand, within Psyr_3720 at 4408.880 kb on + strand, within Psyr_3720 at 4408.880 kb on + strand, within Psyr_3720 at 4408.880 kb on + strand, within Psyr_3720 at 4408.997 kb on + strand, within Psyr_3720 at 4408.997 kb on + strand, within Psyr_3720 at 4408.998 kb on - strand, within Psyr_3720 at 4409.076 kb on - strand, within Psyr_3720 at 4409.157 kb on + strand, within Psyr_3720 at 4409.157 kb on + strand, within Psyr_3720 at 4409.158 kb on - strand, within Psyr_3720 at 4409.158 kb on - strand, within Psyr_3720 at 4409.161 kb on + strand, within Psyr_3720 at 4409.162 kb on - strand, within Psyr_3720 at 4409.162 kb on - strand, within Psyr_3720 at 4409.162 kb on - strand, within Psyr_3720 at 4409.162 kb on - strand, within Psyr_3720 at 4409.162 kb on - strand, within Psyr_3720 at 4409.179 kb on + strand, within Psyr_3720 at 4409.180 kb on - strand, within Psyr_3720 at 4409.190 kb on + strand at 4409.192 kb on + strand at 4409.192 kb on + strand at 4409.192 kb on + strand at 4409.193 kb on - strand at 4409.193 kb on - strand at 4409.193 kb on - strand at 4409.193 kb on - strand at 4409.193 kb on - strand at 4409.223 kb on + strand at 4409.223 kb on + strand at 4409.224 kb on - strand at 4409.224 kb on - strand at 4409.224 kb on - strand at 4409.264 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 4,407,353 - +1.6 4,407,361 + -3.4 4,407,362 - +1.2 4,407,440 + +3.4 4,407,441 - +2.5 4,407,508 - +4.5 4,407,508 - +2.0 4,407,515 + +3.4 4,407,516 - -0.8 4,407,516 - -1.4 4,407,517 + -0.3 4,407,517 + +1.5 4,407,517 + +0.9 4,407,517 + -0.6 4,407,518 - -1.9 4,407,518 - +2.5 4,407,518 - -2.2 4,407,518 - +2.0 4,407,535 + Psyr_3718 0.14 -1.7 4,407,535 + Psyr_3718 0.14 -1.5 4,407,535 + Psyr_3718 0.14 +3.5 4,407,542 + Psyr_3718 0.16 +0.4 4,407,543 - Psyr_3718 0.16 -2.6 4,407,606 - Psyr_3718 0.34 -4.3 4,407,672 + Psyr_3718 0.52 +0.2 4,407,673 - Psyr_3718 0.53 -1.6 4,407,707 + Psyr_3718 0.62 -2.1 4,407,707 + Psyr_3718 0.62 -0.3 4,407,707 + Psyr_3718 0.62 -2.6 4,407,707 + Psyr_3718 0.62 +1.5 4,407,708 - Psyr_3718 0.62 +4.4 4,407,708 - Psyr_3718 0.62 -1.0 4,407,708 - Psyr_3718 0.62 +3.8 4,407,816 + -3.4 4,407,816 + -1.5 4,407,817 - -1.5 4,407,817 - -0.5 4,407,817 - +1.5 4,407,822 + -0.8 4,407,823 - +1.0 4,407,831 + -2.2 4,407,831 + -0.8 4,407,832 - +3.7 4,407,832 - -1.6 4,407,856 + -1.0 4,407,856 + +3.9 4,407,856 + +3.3 4,407,856 + -0.5 4,407,856 + -0.3 4,407,856 + -0.5 4,407,856 + -1.2 4,407,856 + -3.0 4,407,856 + +2.3 4,407,856 + -2.6 4,407,856 + +1.3 4,407,856 + +0.4 4,407,857 - +2.8 4,407,857 - -0.0 4,407,857 - -0.8 4,407,857 - -0.0 4,407,857 - -1.3 4,407,859 + +2.8 4,407,859 + +2.8 4,407,860 - +0.0 4,407,860 - -0.7 4,407,860 - +1.5 4,407,871 - -0.5 4,407,897 + +1.1 4,407,944 - +3.3 4,407,974 + +1.5 4,407,974 + +2.5 4,407,974 + +0.4 4,407,974 + +1.0 4,407,975 - -2.8 4,408,079 + Psyr_3719 0.35 -0.1 4,408,079 + Psyr_3719 0.35 -1.9 4,408,080 - Psyr_3719 0.35 +3.0 4,408,080 - Psyr_3719 0.35 -1.9 4,408,089 - Psyr_3719 0.38 -2.0 4,408,089 - Psyr_3719 0.38 -1.1 4,408,097 + Psyr_3719 0.40 +0.5 4,408,142 + Psyr_3719 0.52 -0.4 4,408,143 - Psyr_3719 0.53 +1.0 4,408,160 + Psyr_3719 0.57 +0.1 4,408,187 - Psyr_3719 0.65 -0.3 4,408,187 - Psyr_3719 0.65 -2.8 4,408,187 - Psyr_3719 0.65 -2.7 4,408,219 + Psyr_3719 0.74 -0.5 4,408,219 + Psyr_3719 0.74 +0.3 4,408,219 + Psyr_3719 0.74 +2.0 4,408,219 + Psyr_3719 0.74 -0.5 4,408,219 + Psyr_3719 0.74 +1.0 4,408,219 + Psyr_3719 0.74 +2.2 4,408,219 + Psyr_3719 0.74 +2.0 4,408,219 + Psyr_3719 0.74 -0.9 4,408,220 - Psyr_3719 0.74 +3.8 4,408,220 - Psyr_3719 0.74 +0.4 4,408,220 - Psyr_3719 0.74 -1.9 4,408,220 - Psyr_3719 0.74 +3.1 4,408,263 + Psyr_3719 0.86 +2.3 4,408,263 + Psyr_3719 0.86 -1.0 4,408,263 + Psyr_3719 0.86 +2.0 4,408,263 + Psyr_3719 0.86 -0.3 4,408,263 + Psyr_3719 0.86 +2.3 4,408,263 + Psyr_3719 0.86 +2.0 4,408,263 + Psyr_3719 0.86 +4.5 4,408,264 - Psyr_3719 0.86 +2.3 4,408,264 - Psyr_3719 0.86 -2.6 4,408,264 - Psyr_3719 0.86 +3.3 4,408,264 - Psyr_3719 0.86 -0.5 4,408,264 - Psyr_3719 0.86 -1.0 4,408,264 - Psyr_3719 0.86 -2.7 4,408,264 - Psyr_3719 0.86 -1.4 4,408,264 - Psyr_3719 0.86 -1.0 4,408,264 - Psyr_3719 0.86 +3.0 4,408,264 - Psyr_3719 0.86 +2.5 4,408,268 - Psyr_3719 0.87 -2.6 4,408,268 - Psyr_3719 0.87 -0.6 4,408,311 + -0.9 4,408,312 - -2.8 4,408,330 - -0.1 4,408,331 + +1.5 4,408,331 + -2.4 4,408,331 + +0.3 4,408,331 + -0.6 4,408,331 + +1.0 4,408,331 + -4.1 4,408,331 + +1.5 4,408,331 + -1.5 4,408,331 + -0.6 4,408,331 + -3.4 4,408,331 + -1.9 4,408,331 + +1.0 4,408,331 + -0.0 4,408,331 + +1.5 4,408,331 + +1.5 4,408,331 + -0.0 4,408,331 + -1.3 4,408,332 - +1.5 4,408,332 - +2.5 4,408,332 - +2.5 4,408,332 - +2.0 4,408,332 - +0.5 4,408,332 - +3.8 4,408,332 - +1.2 4,408,332 - +2.3 4,408,332 - -2.0 4,408,332 - -1.4 4,408,332 - -0.5 4,408,332 - -1.2 4,408,332 - -1.8 4,408,332 - -0.4 4,408,332 - +2.8 4,408,332 - -0.0 4,408,332 - +0.8 4,408,385 - -0.9 4,408,385 - +0.2 4,408,431 + +3.3 4,408,431 + +0.1 4,408,431 + -0.9 4,408,432 - -0.8 4,408,526 + +1.5 4,408,526 + -0.0 4,408,648 + -0.0 4,408,648 + -4.4 4,408,648 + -1.0 4,408,648 + -0.0 4,408,648 + -0.0 4,408,649 - +0.3 4,408,689 + +1.5 4,408,727 + Psyr_3720 0.15 +1.0 4,408,727 + Psyr_3720 0.15 -4.3 4,408,728 - Psyr_3720 0.15 +0.5 4,408,728 - Psyr_3720 0.15 +2.5 4,408,728 - Psyr_3720 0.15 -0.5 4,408,807 + Psyr_3720 0.28 -3.4 4,408,808 - Psyr_3720 0.28 -2.6 4,408,816 + Psyr_3720 0.30 +2.0 4,408,816 + -0.0 4,408,816 + Psyr_3720 0.30 -0.0 4,408,817 - Psyr_3720 0.30 +0.2 4,408,817 - Psyr_3720 0.30 -2.9 4,408,846 + Psyr_3720 0.35 -0.0 4,408,847 - Psyr_3720 0.35 +2.0 4,408,865 + Psyr_3720 0.38 -3.0 4,408,866 - Psyr_3720 0.38 -1.2 4,408,874 + Psyr_3720 0.39 -2.0 4,408,875 - Psyr_3720 0.39 +2.0 4,408,875 - Psyr_3720 0.39 +3.7 4,408,875 - Psyr_3720 0.39 -1.9 4,408,875 - Psyr_3720 0.39 +1.5 4,408,875 - Psyr_3720 0.39 +0.8 4,408,880 + Psyr_3720 0.40 +1.5 4,408,880 + Psyr_3720 0.40 -0.9 4,408,880 + Psyr_3720 0.40 -1.2 4,408,997 + Psyr_3720 0.60 +1.0 4,408,997 + Psyr_3720 0.60 -1.2 4,408,998 - Psyr_3720 0.60 -1.2 4,409,076 - Psyr_3720 0.73 +3.7 4,409,157 + Psyr_3720 0.86 +2.0 4,409,157 + Psyr_3720 0.86 +2.0 4,409,158 - Psyr_3720 0.86 -3.1 4,409,158 - Psyr_3720 0.86 -4.5 4,409,161 + Psyr_3720 0.87 -1.8 4,409,162 - Psyr_3720 0.87 -1.9 4,409,162 - Psyr_3720 0.87 -0.0 4,409,162 - Psyr_3720 0.87 +4.0 4,409,162 - Psyr_3720 0.87 +2.3 4,409,162 - Psyr_3720 0.87 +2.3 4,409,179 + Psyr_3720 0.90 +1.5 4,409,180 - Psyr_3720 0.90 +1.1 4,409,190 + +2.3 4,409,192 + +2.0 4,409,192 + -1.3 4,409,192 + +3.3 4,409,193 - -2.4 4,409,193 - -2.4 4,409,193 - +1.0 4,409,193 - -2.0 4,409,193 - -0.0 4,409,223 + -0.0 4,409,223 + -0.0 4,409,224 - -0.0 4,409,224 - +2.5 4,409,224 - -2.6 4,409,264 - +2.0
Or see this region's nucleotide sequence