Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3683 and Psyr_3684 are separated by 89 nucleotides Psyr_3684 and Psyr_3685 are separated by 75 nucleotides Psyr_3685 and Psyr_3686 are separated by 14 nucleotides Psyr_3686 and Psyr_3687 are separated by 135 nucleotides
Psyr_3683: Psyr_3683 - NLP/P60, at 4,369,999 to 4,370,544
_3683
Psyr_3684: Psyr_3684 - NLP/P60, at 4,370,634 to 4,371,362
_3684
Psyr_3685: Psyr_3685 - Silent information regulator protein Sir2, at 4,371,438 to 4,372,181
_3685
Psyr_3686: Psyr_3686 - regulatory inactivation of DnaA Hda protein, at 4,372,196 to 4,372,981
_3686
Psyr_3687: Psyr_3687 - Protein of unknown function UPF0118, at 4,373,117 to 4,374,190
_3687
Position (kb) 4371
4372
4373 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 4370.544 kb on + strand at 4370.558 kb on + strand at 4370.573 kb on + strand at 4370.574 kb on - strand at 4370.574 kb on - strand at 4370.631 kb on + strand at 4370.632 kb on - strand at 4370.635 kb on + strand at 4370.635 kb on + strand at 4370.635 kb on + strand at 4370.636 kb on - strand at 4370.667 kb on - strand at 4370.713 kb on - strand, within Psyr_3684 at 4370.855 kb on - strand, within Psyr_3684 at 4371.130 kb on + strand, within Psyr_3684 at 4371.290 kb on + strand at 4371.290 kb on + strand at 4371.291 kb on - strand at 4371.382 kb on + strand at 4371.386 kb on + strand at 4371.386 kb on + strand at 4371.386 kb on + strand at 4371.386 kb on + strand at 4371.400 kb on + strand at 4371.401 kb on - strand at 4371.410 kb on - strand at 4371.450 kb on + strand at 4371.719 kb on + strand, within Psyr_3685 at 4371.720 kb on - strand, within Psyr_3685 at 4371.733 kb on - strand, within Psyr_3685 at 4371.733 kb on - strand, within Psyr_3685 at 4371.865 kb on + strand, within Psyr_3685 at 4371.931 kb on + strand, within Psyr_3685 at 4371.932 kb on - strand, within Psyr_3685 at 4371.985 kb on + strand, within Psyr_3685 at 4371.985 kb on + strand, within Psyr_3685 at 4371.985 kb on + strand, within Psyr_3685 at 4371.986 kb on - strand, within Psyr_3685 at 4371.986 kb on - strand, within Psyr_3685 at 4371.986 kb on - strand, within Psyr_3685 at 4372.072 kb on + strand, within Psyr_3685 at 4372.072 kb on + strand, within Psyr_3685 at 4372.073 kb on - strand, within Psyr_3685 at 4372.073 kb on - strand, within Psyr_3685 at 4372.073 kb on - strand, within Psyr_3685 at 4372.094 kb on - strand, within Psyr_3685 at 4372.197 kb on + strand at 4372.197 kb on + strand at 4372.958 kb on - strand at 4373.118 kb on + strand at 4373.119 kb on - strand at 4373.145 kb on + strand at 4373.145 kb on + strand at 4373.146 kb on - strand at 4373.163 kb on + strand at 4373.163 kb on + strand at 4373.164 kb on - strand at 4373.164 kb on - strand at 4373.164 kb on - strand at 4373.164 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 4,370,544 + +1.5 4,370,558 + -0.9 4,370,573 + +0.1 4,370,574 - +2.8 4,370,574 - +2.5 4,370,631 + +2.8 4,370,632 - +3.6 4,370,635 + +2.3 4,370,635 + +0.9 4,370,635 + -0.1 4,370,636 - -0.2 4,370,667 - -1.5 4,370,713 - Psyr_3684 0.11 +3.3 4,370,855 - Psyr_3684 0.30 +1.9 4,371,130 + Psyr_3684 0.68 +1.9 4,371,290 + -0.4 4,371,290 + +2.3 4,371,291 - +0.0 4,371,382 + +1.9 4,371,386 + -2.9 4,371,386 + -0.7 4,371,386 + +0.2 4,371,386 + +1.5 4,371,400 + -1.6 4,371,401 - +2.3 4,371,410 - +1.5 4,371,450 + -1.6 4,371,719 + Psyr_3685 0.38 -0.9 4,371,720 - Psyr_3685 0.38 +1.5 4,371,733 - Psyr_3685 0.40 -1.9 4,371,733 - Psyr_3685 0.40 -2.0 4,371,865 + Psyr_3685 0.57 -0.4 4,371,931 + Psyr_3685 0.66 -0.1 4,371,932 - Psyr_3685 0.66 -2.3 4,371,985 + Psyr_3685 0.74 -0.1 4,371,985 + Psyr_3685 0.74 -2.0 4,371,985 + Psyr_3685 0.74 -4.1 4,371,986 - Psyr_3685 0.74 -1.4 4,371,986 - Psyr_3685 0.74 +1.1 4,371,986 - Psyr_3685 0.74 -1.7 4,372,072 + Psyr_3685 0.85 -1.8 4,372,072 + Psyr_3685 0.85 +2.8 4,372,073 - Psyr_3685 0.85 -0.1 4,372,073 - Psyr_3685 0.85 -2.1 4,372,073 - Psyr_3685 0.85 -0.7 4,372,094 - Psyr_3685 0.88 -1.4 4,372,197 + -2.5 4,372,197 + +4.1 4,372,958 - +1.9 4,373,118 + -2.4 4,373,119 - +2.3 4,373,145 + -4.3 4,373,145 + -0.1 4,373,146 - +3.5 4,373,163 + +1.5 4,373,163 + +0.4 4,373,164 - +1.6 4,373,164 - -1.1 4,373,164 - -0.1 4,373,164 - +3.3
Or see this region's nucleotide sequence