Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3676 and Psyr_3677 overlap by 4 nucleotides Psyr_3677 and Psyr_3678 overlap by 8 nucleotides Psyr_3678 and Psyr_3679 overlap by 4 nucleotides
Psyr_3676: Psyr_3676 - adenosylcobyric acid synthase (glutamine-hydrolysing), at 4,361,979 to 4,363,439
_3676
Psyr_3677: Psyr_3677 - Aminotransferase, class I and II, at 4,363,436 to 4,364,440
_3677
Psyr_3678: Psyr_3678 - adenosylcobinamide-phosphate synthase, at 4,364,433 to 4,365,341
_3678
Psyr_3679: Psyr_3679 - cob(II)yrinic acid a,c-diamide reductase, at 4,365,338 to 4,365,988
_3679
Position (kb) 4363
4364
4365 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 4362.526 kb on + strand, within Psyr_3676 at 4362.527 kb on - strand, within Psyr_3676 at 4362.527 kb on - strand, within Psyr_3676 at 4362.660 kb on - strand, within Psyr_3676 at 4362.767 kb on - strand, within Psyr_3676 at 4363.067 kb on - strand, within Psyr_3676 at 4363.121 kb on + strand, within Psyr_3676 at 4363.122 kb on - strand, within Psyr_3676 at 4363.122 kb on - strand, within Psyr_3676 at 4363.422 kb on + strand at 4363.423 kb on - strand at 4363.423 kb on - strand at 4363.423 kb on - strand at 4363.423 kb on - strand at 4363.423 kb on - strand at 4363.443 kb on + strand at 4363.443 kb on + strand at 4364.082 kb on + strand, within Psyr_3677 at 4364.102 kb on + strand, within Psyr_3677 at 4364.102 kb on + strand, within Psyr_3677 at 4364.103 kb on - strand, within Psyr_3677 at 4364.259 kb on + strand, within Psyr_3677 at 4364.259 kb on + strand, within Psyr_3677 at 4364.260 kb on - strand, within Psyr_3677 at 4364.260 kb on - strand, within Psyr_3677 at 4364.260 kb on - strand, within Psyr_3677 at 4364.475 kb on + strand at 4364.578 kb on + strand, within Psyr_3678 at 4364.745 kb on - strand, within Psyr_3678 at 4364.746 kb on + strand, within Psyr_3678 at 4364.746 kb on + strand, within Psyr_3678 at 4364.746 kb on + strand, within Psyr_3678 at 4364.746 kb on + strand, within Psyr_3678 at 4364.746 kb on + strand, within Psyr_3678 at 4364.746 kb on + strand, within Psyr_3678 at 4364.746 kb on + strand, within Psyr_3678 at 4364.747 kb on - strand, within Psyr_3678 at 4364.747 kb on - strand, within Psyr_3678 at 4364.747 kb on - strand, within Psyr_3678 at 4364.747 kb on - strand, within Psyr_3678 at 4364.747 kb on - strand, within Psyr_3678 at 4364.747 kb on - strand, within Psyr_3678 at 4364.747 kb on - strand, within Psyr_3678 at 4364.747 kb on - strand, within Psyr_3678 at 4364.747 kb on - strand, within Psyr_3678 at 4364.747 kb on - strand, within Psyr_3678 at 4364.747 kb on - strand, within Psyr_3678 at 4364.831 kb on + strand, within Psyr_3678 at 4364.831 kb on + strand, within Psyr_3678 at 4365.366 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 4,362,526 + Psyr_3676 0.37 +2.5 4,362,527 - Psyr_3676 0.38 -0.7 4,362,527 - Psyr_3676 0.38 +2.3 4,362,660 - Psyr_3676 0.47 +3.3 4,362,767 - Psyr_3676 0.54 +0.8 4,363,067 - Psyr_3676 0.74 +2.3 4,363,121 + Psyr_3676 0.78 +2.5 4,363,122 - Psyr_3676 0.78 +3.9 4,363,122 - Psyr_3676 0.78 +1.3 4,363,422 + +2.9 4,363,423 - +1.9 4,363,423 - -0.6 4,363,423 - -3.1 4,363,423 - -1.3 4,363,423 - -3.6 4,363,443 + +3.6 4,363,443 + -4.9 4,364,082 + Psyr_3677 0.64 -0.6 4,364,102 + Psyr_3677 0.66 +0.9 4,364,102 + Psyr_3677 0.66 +0.9 4,364,103 - Psyr_3677 0.66 +1.9 4,364,259 + Psyr_3677 0.82 +3.3 4,364,259 + Psyr_3677 0.82 +2.3 4,364,260 - Psyr_3677 0.82 +3.6 4,364,260 - Psyr_3677 0.82 +0.9 4,364,260 - Psyr_3677 0.82 -0.1 4,364,475 + +1.9 4,364,578 + Psyr_3678 0.16 +2.8 4,364,745 - Psyr_3678 0.34 +2.3 4,364,746 + Psyr_3678 0.34 -1.1 4,364,746 + Psyr_3678 0.34 +2.5 4,364,746 + Psyr_3678 0.34 +2.8 4,364,746 + Psyr_3678 0.34 +1.5 4,364,746 + Psyr_3678 0.34 -0.6 4,364,746 + Psyr_3678 0.34 -1.2 4,364,746 + Psyr_3678 0.34 +1.5 4,364,747 - Psyr_3678 0.35 +1.9 4,364,747 - Psyr_3678 0.35 -0.9 4,364,747 - Psyr_3678 0.35 +0.5 4,364,747 - Psyr_3678 0.35 +3.9 4,364,747 - Psyr_3678 0.35 -2.1 4,364,747 - Psyr_3678 0.35 +3.3 4,364,747 - Psyr_3678 0.35 -0.1 4,364,747 - Psyr_3678 0.35 +1.9 4,364,747 - Psyr_3678 0.35 -1.6 4,364,747 - Psyr_3678 0.35 +0.5 4,364,747 - Psyr_3678 0.35 +2.8 4,364,831 + Psyr_3678 0.44 -1.7 4,364,831 + Psyr_3678 0.44 -0.7 4,365,366 + -0.1
Or see this region's nucleotide sequence