Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3674 and Psyr_3675 overlap by 4 nucleotides Psyr_3675 and Psyr_3676 are separated by 0 nucleotides
Psyr_3674: Psyr_3674 - Nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase, at 4,360,408 to 4,361,460
_3674
Psyr_3675: Psyr_3675 - adenosylcobinamide kinase, at 4,361,457 to 4,361,978
_3675
Psyr_3676: Psyr_3676 - adenosylcobyric acid synthase (glutamine-hydrolysing), at 4,361,979 to 4,363,439
_3676
Position (kb) 4361
4362 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 4360.490 kb on - strand at 4360.640 kb on - strand, within Psyr_3674 at 4360.715 kb on + strand, within Psyr_3674 at 4360.988 kb on - strand, within Psyr_3674 at 4360.988 kb on - strand, within Psyr_3674 at 4361.229 kb on - strand, within Psyr_3674 at 4361.454 kb on + strand at 4361.454 kb on + strand at 4361.454 kb on + strand at 4361.455 kb on - strand at 4361.455 kb on - strand at 4361.455 kb on - strand at 4361.455 kb on - strand at 4361.460 kb on + strand at 4361.563 kb on + strand, within Psyr_3675 at 4361.563 kb on + strand, within Psyr_3675 at 4361.563 kb on + strand, within Psyr_3675 at 4361.890 kb on + strand, within Psyr_3675 at 4361.890 kb on + strand, within Psyr_3675 at 4361.890 kb on + strand, within Psyr_3675 at 4361.891 kb on - strand, within Psyr_3675 at 4362.423 kb on + strand, within Psyr_3676 at 4362.424 kb on - strand, within Psyr_3676 at 4362.526 kb on + strand, within Psyr_3676 at 4362.527 kb on - strand, within Psyr_3676 at 4362.527 kb on - strand, within Psyr_3676 at 4362.660 kb on - strand, within Psyr_3676 at 4362.767 kb on - strand, within Psyr_3676
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 4,360,490 - -0.3 4,360,640 - Psyr_3674 0.22 +2.7 4,360,715 + Psyr_3674 0.29 +2.9 4,360,988 - Psyr_3674 0.55 +0.3 4,360,988 - Psyr_3674 0.55 +2.3 4,361,229 - Psyr_3674 0.78 +3.5 4,361,454 + -0.7 4,361,454 + +3.7 4,361,454 + +1.9 4,361,455 - -0.4 4,361,455 - -4.0 4,361,455 - -1.1 4,361,455 - +3.7 4,361,460 + +3.1 4,361,563 + Psyr_3675 0.20 -1.5 4,361,563 + Psyr_3675 0.20 +3.6 4,361,563 + Psyr_3675 0.20 +1.0 4,361,890 + Psyr_3675 0.83 -1.6 4,361,890 + Psyr_3675 0.83 -2.1 4,361,890 + Psyr_3675 0.83 +0.9 4,361,891 - Psyr_3675 0.83 +1.5 4,362,423 + Psyr_3676 0.30 +1.3 4,362,424 - Psyr_3676 0.30 +3.1 4,362,526 + Psyr_3676 0.37 +2.5 4,362,527 - Psyr_3676 0.38 -0.7 4,362,527 - Psyr_3676 0.38 +2.3 4,362,660 - Psyr_3676 0.47 +3.3 4,362,767 - Psyr_3676 0.54 +0.8
Or see this region's nucleotide sequence