Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3527 and Psyr_3528 are separated by 75 nucleotides Psyr_3528 and Psyr_3529 are separated by 263 nucleotides Psyr_3529 and Psyr_3530 are separated by 10 nucleotides
Psyr_3527: Psyr_3527 - glutathione-independent formaldehyde dehydrogenase, at 4,203,866 to 4,205,101
_3527
Psyr_3528: Psyr_3528 - hypothetical protein, at 4,205,177 to 4,205,419
_3528
Psyr_3529: Psyr_3529 - Asp/Glu racemase, at 4,205,683 to 4,206,414
_3529
Psyr_3530: Psyr_3530 - transcriptional regulator, GntR family, at 4,206,425 to 4,207,144
_3530
Position (kb) 4205
4206
4207 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 4204.758 kb on + strand, within Psyr_3527 at 4204.758 kb on + strand, within Psyr_3527 at 4204.759 kb on - strand, within Psyr_3527 at 4204.791 kb on + strand, within Psyr_3527 at 4204.794 kb on - strand, within Psyr_3527 at 4204.794 kb on - strand, within Psyr_3527 at 4204.794 kb on - strand, within Psyr_3527 at 4204.794 kb on - strand, within Psyr_3527 at 4205.099 kb on + strand at 4205.099 kb on + strand at 4205.099 kb on + strand at 4205.099 kb on + strand at 4205.099 kb on + strand at 4205.099 kb on + strand at 4205.099 kb on + strand at 4205.099 kb on + strand at 4205.099 kb on + strand at 4205.099 kb on + strand at 4205.099 kb on + strand at 4205.099 kb on + strand at 4205.099 kb on + strand at 4205.100 kb on - strand at 4205.100 kb on - strand at 4205.100 kb on - strand at 4205.100 kb on - strand at 4205.100 kb on - strand at 4205.100 kb on - strand at 4205.100 kb on - strand at 4205.100 kb on - strand at 4205.100 kb on - strand at 4205.100 kb on - strand at 4205.100 kb on - strand at 4205.100 kb on - strand at 4205.100 kb on - strand at 4205.100 kb on - strand at 4205.100 kb on - strand at 4205.100 kb on - strand at 4205.100 kb on - strand at 4205.100 kb on - strand at 4205.100 kb on - strand at 4205.176 kb on + strand at 4205.176 kb on + strand at 4205.176 kb on + strand at 4205.176 kb on + strand at 4205.176 kb on + strand at 4205.176 kb on + strand at 4205.176 kb on + strand at 4205.176 kb on + strand at 4205.176 kb on + strand at 4205.176 kb on + strand at 4205.176 kb on + strand at 4205.176 kb on + strand at 4205.176 kb on + strand at 4205.177 kb on - strand at 4205.177 kb on - strand at 4205.177 kb on - strand at 4205.177 kb on - strand at 4205.177 kb on - strand at 4205.177 kb on - strand at 4205.177 kb on - strand at 4205.177 kb on - strand at 4205.177 kb on - strand at 4205.177 kb on - strand at 4205.177 kb on - strand at 4205.177 kb on - strand at 4205.177 kb on - strand at 4205.177 kb on - strand at 4205.177 kb on - strand at 4205.177 kb on - strand at 4205.177 kb on - strand at 4205.177 kb on - strand at 4205.177 kb on - strand at 4205.177 kb on - strand at 4205.177 kb on - strand at 4205.266 kb on - strand, within Psyr_3528 at 4205.352 kb on - strand, within Psyr_3528 at 4205.352 kb on - strand, within Psyr_3528 at 4205.352 kb on - strand, within Psyr_3528 at 4205.352 kb on - strand, within Psyr_3528 at 4205.352 kb on - strand, within Psyr_3528 at 4205.352 kb on - strand, within Psyr_3528 at 4205.352 kb on - strand, within Psyr_3528 at 4205.352 kb on - strand, within Psyr_3528 at 4205.352 kb on - strand, within Psyr_3528 at 4205.352 kb on - strand, within Psyr_3528 at 4205.352 kb on - strand, within Psyr_3528 at 4205.352 kb on - strand, within Psyr_3528 at 4205.352 kb on - strand, within Psyr_3528 at 4205.352 kb on - strand, within Psyr_3528 at 4205.352 kb on - strand, within Psyr_3528 at 4205.352 kb on - strand, within Psyr_3528 at 4205.438 kb on + strand at 4205.439 kb on - strand at 4205.439 kb on - strand at 4205.439 kb on - strand at 4205.439 kb on - strand at 4205.439 kb on - strand at 4205.439 kb on - strand at 4205.439 kb on - strand at 4205.439 kb on - strand at 4205.439 kb on - strand at 4205.440 kb on + strand at 4205.440 kb on + strand at 4205.441 kb on - strand at 4205.441 kb on - strand at 4205.441 kb on - strand at 4205.441 kb on - strand at 4205.441 kb on - strand at 4205.441 kb on - strand at 4205.441 kb on - strand at 4205.441 kb on - strand at 4205.441 kb on - strand at 4205.441 kb on - strand at 4205.441 kb on - strand at 4205.441 kb on - strand at 4205.441 kb on - strand at 4205.441 kb on - strand at 4205.441 kb on - strand at 4205.441 kb on - strand at 4205.441 kb on - strand at 4205.441 kb on - strand at 4205.441 kb on - strand at 4205.441 kb on - strand at 4205.441 kb on - strand at 4205.441 kb on - strand at 4205.441 kb on - strand at 4205.441 kb on - strand at 4205.441 kb on - strand at 4205.441 kb on - strand at 4205.441 kb on - strand at 4205.441 kb on - strand at 4205.441 kb on - strand at 4205.441 kb on - strand at 4205.441 kb on - strand at 4205.441 kb on - strand at 4205.441 kb on - strand at 4205.441 kb on - strand at 4205.485 kb on + strand at 4205.485 kb on + strand at 4205.486 kb on - strand at 4205.486 kb on - strand at 4205.616 kb on + strand at 4205.617 kb on - strand at 4205.617 kb on - strand at 4205.617 kb on - strand at 4205.617 kb on - strand at 4205.617 kb on - strand at 4205.617 kb on - strand at 4205.617 kb on - strand at 4205.617 kb on - strand at 4205.617 kb on - strand at 4205.617 kb on - strand at 4205.617 kb on - strand at 4205.617 kb on - strand at 4205.617 kb on - strand at 4205.617 kb on - strand at 4205.617 kb on - strand at 4205.617 kb on - strand at 4205.617 kb on - strand at 4205.617 kb on - strand at 4205.617 kb on - strand at 4205.617 kb on - strand at 4205.617 kb on - strand at 4205.617 kb on - strand at 4205.617 kb on - strand at 4205.617 kb on - strand at 4205.617 kb on - strand at 4205.617 kb on - strand at 4205.697 kb on - strand at 4205.989 kb on - strand, within Psyr_3529 at 4206.007 kb on + strand, within Psyr_3529 at 4206.007 kb on + strand, within Psyr_3529 at 4206.008 kb on - strand, within Psyr_3529 at 4206.008 kb on - strand, within Psyr_3529 at 4206.008 kb on - strand, within Psyr_3529 at 4206.008 kb on - strand, within Psyr_3529 at 4206.445 kb on + strand at 4206.616 kb on - strand, within Psyr_3530 at 4206.866 kb on + strand, within Psyr_3530 at 4206.866 kb on + strand, within Psyr_3530 at 4206.866 kb on + strand, within Psyr_3530 at 4206.866 kb on + strand, within Psyr_3530 at 4206.867 kb on - strand, within Psyr_3530 at 4206.867 kb on - strand, within Psyr_3530 at 4206.993 kb on - strand, within Psyr_3530 at 4207.071 kb on + strand, within Psyr_3530 at 4207.072 kb on - strand, within Psyr_3530 at 4207.176 kb on - strand at 4207.237 kb on + strand at 4207.237 kb on + strand at 4207.237 kb on + strand at 4207.237 kb on + strand at 4207.238 kb on - strand at 4207.271 kb on + strand at 4207.271 kb on + strand at 4207.272 kb on - strand at 4207.272 kb on - strand at 4207.272 kb on - strand at 4207.272 kb on - strand at 4207.272 kb on - strand at 4207.272 kb on - strand at 4207.294 kb on + strand at 4207.294 kb on + strand at 4207.400 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 4,204,758 + Psyr_3527 0.72 +0.2 4,204,758 + Psyr_3527 0.72 +4.7 4,204,759 - Psyr_3527 0.72 -2.5 4,204,791 + Psyr_3527 0.75 +1.9 4,204,794 - Psyr_3527 0.75 -1.8 4,204,794 - Psyr_3527 0.75 +2.7 4,204,794 - Psyr_3527 0.75 -1.4 4,204,794 - Psyr_3527 0.75 -0.1 4,205,099 + +4.0 4,205,099 + -2.2 4,205,099 + +0.3 4,205,099 + -0.1 4,205,099 + +2.9 4,205,099 + +1.9 4,205,099 + -0.5 4,205,099 + +1.5 4,205,099 + +0.3 4,205,099 + -3.3 4,205,099 + +0.3 4,205,099 + +3.7 4,205,099 + -0.2 4,205,100 - +3.2 4,205,100 - -0.2 4,205,100 - -0.6 4,205,100 - +3.3 4,205,100 - -0.8 4,205,100 - -0.5 4,205,100 - -0.8 4,205,100 - +3.2 4,205,100 - -2.3 4,205,100 - -1.1 4,205,100 - -1.2 4,205,100 - -2.3 4,205,100 - +1.9 4,205,100 - +0.3 4,205,100 - -1.5 4,205,100 - -0.1 4,205,100 - -3.6 4,205,100 - +3.3 4,205,100 - -0.1 4,205,176 + -0.1 4,205,176 + -1.7 4,205,176 + -0.0 4,205,176 + -2.7 4,205,176 + -1.7 4,205,176 + +0.9 4,205,176 + -1.8 4,205,176 + -1.7 4,205,176 + -1.9 4,205,176 + +3.0 4,205,176 + +1.5 4,205,176 + +1.9 4,205,176 + +0.9 4,205,177 - +1.9 4,205,177 - +1.9 4,205,177 - -0.3 4,205,177 - -2.4 4,205,177 - +2.5 4,205,177 - +1.9 4,205,177 - -1.6 4,205,177 - -1.4 4,205,177 - +0.2 4,205,177 - +0.3 4,205,177 - +1.5 4,205,177 - +0.3 4,205,177 - -3.3 4,205,177 - +4.3 4,205,177 - +2.5 4,205,177 - +2.5 4,205,177 - -1.5 4,205,177 - -0.8 4,205,177 - +0.9 4,205,177 - -4.3 4,205,177 - -0.6 4,205,266 - Psyr_3528 0.37 -0.0 4,205,352 - Psyr_3528 0.72 -0.5 4,205,352 - Psyr_3528 0.72 +1.9 4,205,352 - Psyr_3528 0.72 -2.9 4,205,352 - Psyr_3528 0.72 +4.8 4,205,352 - Psyr_3528 0.72 -1.6 4,205,352 - Psyr_3528 0.72 +3.2 4,205,352 - Psyr_3528 0.72 +2.9 4,205,352 - Psyr_3528 0.72 +2.9 4,205,352 - Psyr_3528 0.72 -0.1 4,205,352 - Psyr_3528 0.72 -2.1 4,205,352 - Psyr_3528 0.72 -0.6 4,205,352 - Psyr_3528 0.72 +2.7 4,205,352 - Psyr_3528 0.72 -0.5 4,205,352 - Psyr_3528 0.72 -0.4 4,205,352 - Psyr_3528 0.72 +2.2 4,205,352 - Psyr_3528 0.72 -2.5 4,205,438 + -0.2 4,205,439 - -1.2 4,205,439 - -0.4 4,205,439 - +2.5 4,205,439 - +1.9 4,205,439 - -3.0 4,205,439 - -2.4 4,205,439 - -1.3 4,205,439 - +3.0 4,205,439 - +2.2 4,205,440 + +1.5 4,205,440 + +2.7 4,205,441 - -0.1 4,205,441 - +3.2 4,205,441 - +1.9 4,205,441 - -1.9 4,205,441 - -1.2 4,205,441 - -1.4 4,205,441 - +3.2 4,205,441 - -0.6 4,205,441 - +3.3 4,205,441 - +2.5 4,205,441 - -2.0 4,205,441 - +2.7 4,205,441 - +2.9 4,205,441 - +4.1 4,205,441 - -0.8 4,205,441 - +4.0 4,205,441 - +2.5 4,205,441 - -0.9 4,205,441 - -0.4 4,205,441 - -0.8 4,205,441 - -0.1 4,205,441 - +4.5 4,205,441 - -0.1 4,205,441 - -1.1 4,205,441 - +1.9 4,205,441 - +3.3 4,205,441 - +3.2 4,205,441 - +2.7 4,205,441 - -0.6 4,205,441 - +3.3 4,205,441 - -1.8 4,205,441 - -2.6 4,205,441 - -3.9 4,205,441 - -3.3 4,205,485 + +2.5 4,205,485 + +0.6 4,205,486 - -0.1 4,205,486 - -0.4 4,205,616 + +3.7 4,205,617 - +3.6 4,205,617 - +0.5 4,205,617 - +2.2 4,205,617 - -1.0 4,205,617 - -0.0 4,205,617 - -3.3 4,205,617 - +0.4 4,205,617 - -1.2 4,205,617 - -0.1 4,205,617 - +4.5 4,205,617 - +0.7 4,205,617 - -0.1 4,205,617 - -0.1 4,205,617 - -0.1 4,205,617 - -0.3 4,205,617 - -1.1 4,205,617 - -2.0 4,205,617 - -1.7 4,205,617 - -1.9 4,205,617 - +2.9 4,205,617 - -1.1 4,205,617 - +0.4 4,205,617 - -0.7 4,205,617 - +0.0 4,205,617 - -1.9 4,205,617 - +1.5 4,205,697 - +0.2 4,205,989 - Psyr_3529 0.42 +2.5 4,206,007 + Psyr_3529 0.44 +2.7 4,206,007 + Psyr_3529 0.44 -2.0 4,206,008 - Psyr_3529 0.44 -1.6 4,206,008 - Psyr_3529 0.44 -1.2 4,206,008 - Psyr_3529 0.44 +3.3 4,206,008 - Psyr_3529 0.44 -1.6 4,206,445 + +3.3 4,206,616 - Psyr_3530 0.27 -0.2 4,206,866 + Psyr_3530 0.61 -2.7 4,206,866 + Psyr_3530 0.61 +2.3 4,206,866 + Psyr_3530 0.61 -0.3 4,206,866 + Psyr_3530 0.61 -0.4 4,206,867 - Psyr_3530 0.61 +1.5 4,206,867 - Psyr_3530 0.61 -0.9 4,206,993 - Psyr_3530 0.79 -0.1 4,207,071 + Psyr_3530 0.90 +2.5 4,207,072 - Psyr_3530 0.90 +3.2 4,207,176 - +1.6 4,207,237 + -2.6 4,207,237 + +2.2 4,207,237 + +2.5 4,207,237 + +0.9 4,207,238 - -0.5 4,207,271 + +0.9 4,207,271 + -0.9 4,207,272 - +2.5 4,207,272 - +1.5 4,207,272 - -2.0 4,207,272 - -0.8 4,207,272 - -2.2 4,207,272 - +3.7 4,207,294 + +2.2 4,207,294 + -1.8 4,207,400 + -2.2
Or see this region's nucleotide sequence