Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3522 and Psyr_3523 are separated by 140 nucleotides Psyr_3523 and Psyr_3524 are separated by 4 nucleotides
Psyr_3522: Psyr_3522 - L-serine ammonia-lyase, at 4,197,604 to 4,198,980
_3522
Psyr_3523: Psyr_3523 - Peptidase M20D, amidohydrolase, at 4,199,121 to 4,200,278
_3523
Psyr_3524: Psyr_3524 - Aldehyde dehydrogenase (NAD+), at 4,200,283 to 4,201,800
_3524
Position (kb)
4199
4200
4201 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 4198.373 kb on + strand, within Psyr_3522 at 4198.374 kb on - strand, within Psyr_3522 at 4198.510 kb on + strand, within Psyr_3522 at 4198.510 kb on + strand, within Psyr_3522 at 4198.510 kb on + strand, within Psyr_3522 at 4198.511 kb on - strand, within Psyr_3522 at 4198.511 kb on - strand, within Psyr_3522 at 4198.511 kb on - strand, within Psyr_3522 at 4198.511 kb on - strand, within Psyr_3522 at 4198.511 kb on - strand, within Psyr_3522 at 4198.643 kb on + strand, within Psyr_3522 at 4198.644 kb on - strand, within Psyr_3522 at 4198.710 kb on - strand, within Psyr_3522 at 4198.740 kb on + strand, within Psyr_3522 at 4198.741 kb on - strand, within Psyr_3522 at 4198.930 kb on + strand at 4198.930 kb on + strand at 4198.930 kb on + strand at 4198.930 kb on + strand at 4198.931 kb on - strand at 4198.931 kb on - strand at 4198.931 kb on - strand at 4199.006 kb on - strand at 4199.027 kb on + strand at 4199.027 kb on + strand at 4199.027 kb on + strand at 4199.028 kb on - strand at 4199.028 kb on - strand at 4199.074 kb on + strand at 4199.074 kb on + strand at 4199.074 kb on + strand at 4199.082 kb on - strand at 4199.201 kb on - strand at 4199.203 kb on + strand at 4199.257 kb on + strand, within Psyr_3523 at 4199.257 kb on + strand, within Psyr_3523 at 4199.257 kb on + strand, within Psyr_3523 at 4199.257 kb on + strand, within Psyr_3523 at 4199.257 kb on + strand, within Psyr_3523 at 4199.257 kb on + strand, within Psyr_3523 at 4199.257 kb on + strand, within Psyr_3523 at 4199.257 kb on + strand, within Psyr_3523 at 4199.257 kb on + strand, within Psyr_3523 at 4199.258 kb on - strand, within Psyr_3523 at 4199.258 kb on - strand, within Psyr_3523 at 4199.258 kb on - strand, within Psyr_3523 at 4199.258 kb on - strand, within Psyr_3523 at 4199.258 kb on - strand, within Psyr_3523 at 4199.258 kb on - strand, within Psyr_3523 at 4199.258 kb on - strand, within Psyr_3523 at 4199.419 kb on + strand, within Psyr_3523 at 4199.420 kb on - strand, within Psyr_3523 at 4199.815 kb on + strand, within Psyr_3523 at 4199.816 kb on - strand, within Psyr_3523 at 4200.284 kb on + strand at 4200.284 kb on + strand at 4200.284 kb on + strand at 4200.362 kb on + strand at 4200.362 kb on + strand at 4200.413 kb on + strand at 4200.441 kb on + strand, within Psyr_3524 at 4200.441 kb on + strand at 4200.441 kb on + strand, within Psyr_3524 at 4200.441 kb on + strand, within Psyr_3524 at 4200.441 kb on + strand, within Psyr_3524 at 4200.441 kb on + strand, within Psyr_3524 at 4200.442 kb on - strand, within Psyr_3524 at 4200.442 kb on - strand, within Psyr_3524 at 4200.442 kb on - strand at 4200.449 kb on + strand, within Psyr_3524 at 4200.635 kb on + strand, within Psyr_3524 at 4200.636 kb on - strand, within Psyr_3524 at 4200.636 kb on - strand, within Psyr_3524 at 4200.636 kb on - strand, within Psyr_3524 at 4200.674 kb on + strand, within Psyr_3524 at 4200.674 kb on + strand, within Psyr_3524 at 4200.674 kb on + strand, within Psyr_3524 at 4200.674 kb on + strand, within Psyr_3524 at 4200.674 kb on + strand, within Psyr_3524 at 4200.674 kb on + strand, within Psyr_3524 at 4200.674 kb on + strand, within Psyr_3524 at 4200.674 kb on + strand, within Psyr_3524 at 4200.674 kb on + strand, within Psyr_3524 at 4200.674 kb on + strand, within Psyr_3524 at 4200.675 kb on - strand, within Psyr_3524 at 4200.675 kb on - strand, within Psyr_3524 at 4200.675 kb on - strand, within Psyr_3524 at 4200.675 kb on - strand, within Psyr_3524 at 4200.675 kb on - strand, within Psyr_3524 at 4200.675 kb on - strand, within Psyr_3524 at 4200.675 kb on - strand, within Psyr_3524 at 4200.675 kb on - strand, within Psyr_3524 at 4200.675 kb on - strand, within Psyr_3524 at 4200.675 kb on - strand, within Psyr_3524 at 4200.675 kb on - strand, within Psyr_3524 at 4200.770 kb on + strand, within Psyr_3524 at 4200.770 kb on + strand, within Psyr_3524 at 4200.770 kb on + strand, within Psyr_3524 at 4200.770 kb on + strand, within Psyr_3524 at 4200.771 kb on - strand, within Psyr_3524 at 4200.771 kb on - strand, within Psyr_3524
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 4,198,373 + Psyr_3522 0.56 -0.4 4,198,374 - Psyr_3522 0.56 +2.2 4,198,510 + Psyr_3522 0.66 +1.9 4,198,510 + Psyr_3522 0.66 -3.2 4,198,510 + Psyr_3522 0.66 +2.9 4,198,511 - Psyr_3522 0.66 -1.5 4,198,511 - Psyr_3522 0.66 -0.5 4,198,511 - Psyr_3522 0.66 +2.5 4,198,511 - Psyr_3522 0.66 +0.2 4,198,511 - Psyr_3522 0.66 +3.5 4,198,643 + Psyr_3522 0.75 +2.9 4,198,644 - Psyr_3522 0.76 -3.6 4,198,710 - Psyr_3522 0.80 +2.9 4,198,740 + Psyr_3522 0.82 +1.9 4,198,741 - Psyr_3522 0.83 +0.9 4,198,930 + +0.2 4,198,930 + -0.9 4,198,930 + +2.7 4,198,930 + +1.5 4,198,931 - -0.1 4,198,931 - +2.2 4,198,931 - -0.4 4,199,006 - +3.7 4,199,027 + -0.1 4,199,027 + +1.9 4,199,027 + -1.1 4,199,028 - -0.7 4,199,028 - -0.7 4,199,074 + -0.5 4,199,074 + -0.9 4,199,074 + -0.3 4,199,082 - -2.6 4,199,201 - +1.2 4,199,203 + +3.9 4,199,257 + Psyr_3523 0.12 -2.1 4,199,257 + Psyr_3523 0.12 -1.3 4,199,257 + Psyr_3523 0.12 +3.3 4,199,257 + Psyr_3523 0.12 -1.0 4,199,257 + Psyr_3523 0.12 -3.9 4,199,257 + Psyr_3523 0.12 -0.5 4,199,257 + Psyr_3523 0.12 +1.9 4,199,257 + Psyr_3523 0.12 -0.9 4,199,257 + Psyr_3523 0.12 +3.5 4,199,258 - Psyr_3523 0.12 -1.4 4,199,258 - Psyr_3523 0.12 +0.9 4,199,258 - Psyr_3523 0.12 +2.9 4,199,258 - Psyr_3523 0.12 +3.9 4,199,258 - Psyr_3523 0.12 -0.4 4,199,258 - Psyr_3523 0.12 -3.0 4,199,258 - Psyr_3523 0.12 +2.7 4,199,419 + Psyr_3523 0.26 -0.7 4,199,420 - Psyr_3523 0.26 -1.5 4,199,815 + Psyr_3523 0.60 +3.6 4,199,816 - Psyr_3523 0.60 -0.1 4,200,284 + +0.9 4,200,284 + -3.0 4,200,284 + -2.8 4,200,362 + +4.0 4,200,362 + +0.9 4,200,413 + -1.8 4,200,441 + Psyr_3524 0.10 +2.2 4,200,441 + -3.4 4,200,441 + Psyr_3524 0.10 +0.9 4,200,441 + Psyr_3524 0.10 -0.1 4,200,441 + Psyr_3524 0.10 +0.9 4,200,441 + Psyr_3524 0.10 -0.8 4,200,442 - Psyr_3524 0.10 -0.4 4,200,442 - Psyr_3524 0.10 -0.3 4,200,442 - +1.5 4,200,449 + Psyr_3524 0.11 -0.1 4,200,635 + Psyr_3524 0.23 +2.5 4,200,636 - Psyr_3524 0.23 -0.8 4,200,636 - Psyr_3524 0.23 -0.7 4,200,636 - Psyr_3524 0.23 -1.5 4,200,674 + Psyr_3524 0.26 +2.2 4,200,674 + Psyr_3524 0.26 +1.9 4,200,674 + Psyr_3524 0.26 -0.1 4,200,674 + Psyr_3524 0.26 -4.1 4,200,674 + Psyr_3524 0.26 -0.9 4,200,674 + Psyr_3524 0.26 -1.1 4,200,674 + Psyr_3524 0.26 -1.6 4,200,674 + Psyr_3524 0.26 -1.6 4,200,674 + Psyr_3524 0.26 +0.6 4,200,674 + Psyr_3524 0.26 +2.7 4,200,675 - Psyr_3524 0.26 +2.7 4,200,675 - Psyr_3524 0.26 +0.9 4,200,675 - Psyr_3524 0.26 -1.0 4,200,675 - Psyr_3524 0.26 -2.3 4,200,675 - Psyr_3524 0.26 +0.7 4,200,675 - Psyr_3524 0.26 -2.1 4,200,675 - Psyr_3524 0.26 -3.1 4,200,675 - Psyr_3524 0.26 -0.8 4,200,675 - Psyr_3524 0.26 -0.6 4,200,675 - Psyr_3524 0.26 -0.7 4,200,675 - Psyr_3524 0.26 -0.5 4,200,770 + Psyr_3524 0.32 +1.7 4,200,770 + Psyr_3524 0.32 -1.0 4,200,770 + Psyr_3524 0.32 +0.5 4,200,770 + Psyr_3524 0.32 -0.1 4,200,771 - Psyr_3524 0.32 +4.0 4,200,771 - Psyr_3524 0.32 -1.2
Or see this region's nucleotide sequence