Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3491 and Psyr_3492 are separated by 54 nucleotides Psyr_3492 and Psyr_3493 are separated by 100 nucleotides
Psyr_3491: Psyr_3491 - Major facilitator superfamily, at 4,159,382 to 4,160,803
_3491
Psyr_3492: Psyr_3492 - L-glutamine synthetase, at 4,160,858 to 4,162,201
_3492
Psyr_3493: Psyr_3493 - conserved hypothetical protein, at 4,162,302 to 4,163,213
_3493
Position (kb)
4160
4161
4162
4163 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 4160.002 kb on + strand, within Psyr_3491 at 4160.002 kb on + strand, within Psyr_3491 at 4160.002 kb on + strand, within Psyr_3491 at 4160.003 kb on - strand, within Psyr_3491 at 4160.003 kb on - strand, within Psyr_3491 at 4160.134 kb on - strand, within Psyr_3491 at 4160.183 kb on + strand, within Psyr_3491 at 4160.373 kb on - strand, within Psyr_3491 at 4160.385 kb on + strand, within Psyr_3491 at 4160.386 kb on - strand, within Psyr_3491 at 4160.386 kb on - strand, within Psyr_3491 at 4160.400 kb on + strand, within Psyr_3491 at 4160.469 kb on + strand, within Psyr_3491 at 4160.469 kb on + strand, within Psyr_3491 at 4160.469 kb on + strand, within Psyr_3491 at 4160.469 kb on + strand, within Psyr_3491 at 4160.469 kb on + strand, within Psyr_3491 at 4160.469 kb on + strand, within Psyr_3491 at 4160.469 kb on + strand, within Psyr_3491 at 4160.469 kb on + strand, within Psyr_3491 at 4160.469 kb on + strand, within Psyr_3491 at 4160.469 kb on + strand, within Psyr_3491 at 4160.469 kb on + strand, within Psyr_3491 at 4160.470 kb on - strand, within Psyr_3491 at 4160.470 kb on - strand, within Psyr_3491 at 4160.470 kb on - strand, within Psyr_3491 at 4160.470 kb on - strand, within Psyr_3491 at 4160.470 kb on - strand, within Psyr_3491 at 4160.470 kb on - strand, within Psyr_3491 at 4160.470 kb on - strand, within Psyr_3491 at 4160.470 kb on - strand, within Psyr_3491 at 4160.470 kb on - strand, within Psyr_3491 at 4160.470 kb on - strand, within Psyr_3491 at 4160.470 kb on - strand, within Psyr_3491 at 4160.470 kb on - strand, within Psyr_3491 at 4160.594 kb on - strand, within Psyr_3491 at 4160.652 kb on + strand, within Psyr_3491 at 4160.652 kb on + strand, within Psyr_3491 at 4160.652 kb on + strand, within Psyr_3491 at 4160.653 kb on - strand, within Psyr_3491 at 4160.653 kb on - strand, within Psyr_3491 at 4160.653 kb on - strand, within Psyr_3491 at 4160.721 kb on + strand at 4160.721 kb on + strand at 4160.721 kb on + strand at 4160.721 kb on + strand at 4160.721 kb on + strand at 4160.721 kb on + strand at 4160.722 kb on - strand at 4160.769 kb on + strand at 4160.769 kb on + strand at 4160.898 kb on - strand at 4160.943 kb on + strand at 4160.944 kb on - strand at 4160.944 kb on - strand at 4161.022 kb on + strand, within Psyr_3492 at 4161.023 kb on - strand, within Psyr_3492 at 4161.023 kb on - strand, within Psyr_3492 at 4161.138 kb on + strand, within Psyr_3492 at 4161.209 kb on + strand, within Psyr_3492 at 4161.209 kb on + strand, within Psyr_3492 at 4161.210 kb on - strand, within Psyr_3492 at 4161.262 kb on - strand, within Psyr_3492 at 4161.392 kb on + strand, within Psyr_3492 at 4161.392 kb on + strand, within Psyr_3492 at 4161.392 kb on + strand, within Psyr_3492 at 4161.393 kb on - strand, within Psyr_3492 at 4161.393 kb on - strand, within Psyr_3492 at 4161.393 kb on - strand, within Psyr_3492 at 4161.393 kb on - strand, within Psyr_3492 at 4161.651 kb on + strand, within Psyr_3492 at 4161.651 kb on + strand, within Psyr_3492 at 4161.651 kb on + strand, within Psyr_3492 at 4161.651 kb on + strand, within Psyr_3492 at 4161.651 kb on + strand, within Psyr_3492 at 4161.651 kb on + strand, within Psyr_3492 at 4161.651 kb on + strand, within Psyr_3492 at 4161.651 kb on + strand, within Psyr_3492 at 4161.652 kb on - strand, within Psyr_3492 at 4161.652 kb on - strand, within Psyr_3492 at 4161.652 kb on - strand, within Psyr_3492 at 4161.652 kb on - strand, within Psyr_3492 at 4161.652 kb on - strand, within Psyr_3492 at 4161.652 kb on - strand, within Psyr_3492 at 4161.652 kb on - strand, within Psyr_3492 at 4161.652 kb on - strand, within Psyr_3492 at 4161.835 kb on - strand, within Psyr_3492 at 4162.003 kb on - strand, within Psyr_3492 at 4162.101 kb on - strand at 4162.137 kb on - strand at 4162.215 kb on + strand at 4162.215 kb on + strand at 4162.215 kb on + strand at 4162.215 kb on + strand at 4162.216 kb on - strand at 4162.491 kb on + strand, within Psyr_3493 at 4162.491 kb on + strand, within Psyr_3493 at 4162.492 kb on - strand, within Psyr_3493 at 4162.492 kb on - strand, within Psyr_3493 at 4162.492 kb on - strand, within Psyr_3493 at 4162.492 kb on - strand, within Psyr_3493 at 4162.492 kb on - strand, within Psyr_3493 at 4162.492 kb on - strand, within Psyr_3493 at 4162.666 kb on + strand, within Psyr_3493 at 4162.666 kb on + strand, within Psyr_3493 at 4162.667 kb on - strand, within Psyr_3493 at 4162.696 kb on + strand, within Psyr_3493 at 4162.696 kb on + strand, within Psyr_3493 at 4162.696 kb on + strand, within Psyr_3493 at 4162.696 kb on + strand, within Psyr_3493 at 4162.696 kb on + strand, within Psyr_3493 at 4162.697 kb on - strand, within Psyr_3493 at 4162.697 kb on - strand, within Psyr_3493 at 4162.697 kb on - strand, within Psyr_3493 at 4162.697 kb on - strand, within Psyr_3493 at 4162.697 kb on - strand, within Psyr_3493 at 4162.697 kb on - strand, within Psyr_3493 at 4162.878 kb on + strand, within Psyr_3493 at 4162.966 kb on + strand, within Psyr_3493 at 4162.966 kb on + strand, within Psyr_3493 at 4162.967 kb on - strand, within Psyr_3493 at 4163.062 kb on + strand, within Psyr_3493 at 4163.062 kb on + strand, within Psyr_3493 at 4163.062 kb on + strand, within Psyr_3493 at 4163.062 kb on + strand, within Psyr_3493 at 4163.062 kb on + strand, within Psyr_3493 at 4163.062 kb on + strand, within Psyr_3493 at 4163.063 kb on - strand, within Psyr_3493 at 4163.063 kb on - strand, within Psyr_3493 at 4163.063 kb on - strand, within Psyr_3493 at 4163.063 kb on - strand, within Psyr_3493 at 4163.071 kb on + strand, within Psyr_3493 at 4163.071 kb on + strand, within Psyr_3493 at 4163.072 kb on - strand, within Psyr_3493 at 4163.072 kb on - strand, within Psyr_3493 at 4163.175 kb on + strand at 4163.175 kb on + strand at 4163.176 kb on - strand at 4163.176 kb on - strand at 4163.195 kb on - strand at 4163.201 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 4,160,002 + Psyr_3491 0.44 -0.2 4,160,002 + Psyr_3491 0.44 +3.3 4,160,002 + Psyr_3491 0.44 -2.3 4,160,003 - Psyr_3491 0.44 -2.5 4,160,003 - Psyr_3491 0.44 -1.2 4,160,134 - Psyr_3491 0.53 -2.2 4,160,183 + Psyr_3491 0.56 -0.2 4,160,373 - Psyr_3491 0.70 +0.2 4,160,385 + Psyr_3491 0.71 +4.5 4,160,386 - Psyr_3491 0.71 +4.7 4,160,386 - Psyr_3491 0.71 -1.3 4,160,400 + Psyr_3491 0.72 +0.6 4,160,469 + Psyr_3491 0.76 -0.2 4,160,469 + Psyr_3491 0.76 -1.3 4,160,469 + Psyr_3491 0.76 +2.8 4,160,469 + Psyr_3491 0.76 +2.1 4,160,469 + Psyr_3491 0.76 -3.2 4,160,469 + Psyr_3491 0.76 +1.1 4,160,469 + Psyr_3491 0.76 -1.5 4,160,469 + Psyr_3491 0.76 -1.4 4,160,469 + Psyr_3491 0.76 +2.4 4,160,469 + Psyr_3491 0.76 +4.1 4,160,469 + Psyr_3491 0.76 +2.1 4,160,470 - Psyr_3491 0.77 +2.1 4,160,470 - Psyr_3491 0.77 -0.6 4,160,470 - Psyr_3491 0.77 +0.8 4,160,470 - Psyr_3491 0.77 +3.6 4,160,470 - Psyr_3491 0.77 +1.8 4,160,470 - Psyr_3491 0.77 -2.3 4,160,470 - Psyr_3491 0.77 +1.4 4,160,470 - Psyr_3491 0.77 -0.5 4,160,470 - Psyr_3491 0.77 +2.0 4,160,470 - Psyr_3491 0.77 +2.6 4,160,470 - Psyr_3491 0.77 -0.8 4,160,470 - Psyr_3491 0.77 -2.1 4,160,594 - Psyr_3491 0.85 +3.3 4,160,652 + Psyr_3491 0.89 -1.8 4,160,652 + Psyr_3491 0.89 -1.0 4,160,652 + Psyr_3491 0.89 +1.4 4,160,653 - Psyr_3491 0.89 +3.3 4,160,653 - Psyr_3491 0.89 -0.1 4,160,653 - Psyr_3491 0.89 -2.9 4,160,721 + -2.1 4,160,721 + +2.8 4,160,721 + -1.0 4,160,721 + +3.3 4,160,721 + -0.2 4,160,721 + -1.2 4,160,722 - +1.4 4,160,769 + -0.7 4,160,769 + -1.5 4,160,898 - -0.2 4,160,943 + -0.5 4,160,944 - +1.1 4,160,944 - -0.6 4,161,022 + Psyr_3492 0.12 +2.1 4,161,023 - Psyr_3492 0.12 +2.1 4,161,023 - Psyr_3492 0.12 -2.5 4,161,138 + Psyr_3492 0.21 +1.0 4,161,209 + Psyr_3492 0.26 +3.0 4,161,209 + Psyr_3492 0.26 +1.0 4,161,210 - Psyr_3492 0.26 -1.7 4,161,262 - Psyr_3492 0.30 -0.9 4,161,392 + Psyr_3492 0.40 +2.1 4,161,392 + Psyr_3492 0.40 +1.4 4,161,392 + Psyr_3492 0.40 -2.8 4,161,393 - Psyr_3492 0.40 +4.0 4,161,393 - Psyr_3492 0.40 +1.4 4,161,393 - Psyr_3492 0.40 +1.8 4,161,393 - Psyr_3492 0.40 +4.5 4,161,651 + Psyr_3492 0.59 -3.6 4,161,651 + Psyr_3492 0.59 -0.8 4,161,651 + Psyr_3492 0.59 +3.3 4,161,651 + Psyr_3492 0.59 -1.3 4,161,651 + Psyr_3492 0.59 +0.3 4,161,651 + Psyr_3492 0.59 +2.8 4,161,651 + Psyr_3492 0.59 -1.5 4,161,651 + Psyr_3492 0.59 +2.8 4,161,652 - Psyr_3492 0.59 -1.5 4,161,652 - Psyr_3492 0.59 +3.3 4,161,652 - Psyr_3492 0.59 +1.4 4,161,652 - Psyr_3492 0.59 -2.1 4,161,652 - Psyr_3492 0.59 +0.5 4,161,652 - Psyr_3492 0.59 -0.2 4,161,652 - Psyr_3492 0.59 -1.5 4,161,652 - Psyr_3492 0.59 -0.1 4,161,835 - Psyr_3492 0.73 -0.5 4,162,003 - Psyr_3492 0.85 -3.0 4,162,101 - -0.6 4,162,137 - -0.6 4,162,215 + -1.0 4,162,215 + +1.4 4,162,215 + -1.9 4,162,215 + +3.0 4,162,216 - +3.4 4,162,491 + Psyr_3493 0.21 -4.0 4,162,491 + Psyr_3493 0.21 -1.5 4,162,492 - Psyr_3493 0.21 +3.3 4,162,492 - Psyr_3493 0.21 -0.9 4,162,492 - Psyr_3493 0.21 -0.7 4,162,492 - Psyr_3493 0.21 -0.1 4,162,492 - Psyr_3493 0.21 +2.1 4,162,492 - Psyr_3493 0.21 -1.7 4,162,666 + Psyr_3493 0.40 +0.6 4,162,666 + Psyr_3493 0.40 -4.1 4,162,667 - Psyr_3493 0.40 -0.5 4,162,696 + Psyr_3493 0.43 +1.1 4,162,696 + Psyr_3493 0.43 -2.9 4,162,696 + Psyr_3493 0.43 -2.6 4,162,696 + Psyr_3493 0.43 +3.5 4,162,696 + Psyr_3493 0.43 +0.4 4,162,697 - Psyr_3493 0.43 +0.3 4,162,697 - Psyr_3493 0.43 -3.4 4,162,697 - Psyr_3493 0.43 +1.4 4,162,697 - Psyr_3493 0.43 +3.0 4,162,697 - Psyr_3493 0.43 -0.4 4,162,697 - Psyr_3493 0.43 -1.5 4,162,878 + Psyr_3493 0.63 +3.4 4,162,966 + Psyr_3493 0.73 -2.7 4,162,966 + Psyr_3493 0.73 -1.5 4,162,967 - Psyr_3493 0.73 +1.4 4,163,062 + Psyr_3493 0.83 +2.6 4,163,062 + Psyr_3493 0.83 -1.4 4,163,062 + Psyr_3493 0.83 -0.2 4,163,062 + Psyr_3493 0.83 -1.0 4,163,062 + Psyr_3493 0.83 +2.4 4,163,062 + Psyr_3493 0.83 -4.2 4,163,063 - Psyr_3493 0.83 +2.6 4,163,063 - Psyr_3493 0.83 +0.0 4,163,063 - Psyr_3493 0.83 -2.9 4,163,063 - Psyr_3493 0.83 +0.8 4,163,071 + Psyr_3493 0.84 +0.6 4,163,071 + Psyr_3493 0.84 +2.1 4,163,072 - Psyr_3493 0.84 +2.1 4,163,072 - Psyr_3493 0.84 -2.1 4,163,175 + +1.4 4,163,175 + +0.8 4,163,176 - +2.8 4,163,176 - -2.2 4,163,195 - +1.8 4,163,201 + -3.0
Or see this region's nucleotide sequence