Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3461 and Psyr_3462 are separated by 177 nucleotides Psyr_3462 and Psyr_3463 are separated by 24 nucleotides Psyr_3463 and Psyr_3464 are separated by 123 nucleotides
Psyr_3461: Psyr_3461 - Helix-turn-helix, Fis-type, at 4,125,202 to 4,126,677
_3461
Psyr_3462: Psyr_3462 - conserved hypothetical protein, at 4,126,855 to 4,127,151
_3462
Psyr_3463: Psyr_3463 - Flagellar protein FliS, at 4,127,176 to 4,127,565
_3463
Psyr_3464: Psyr_3464 - Flagellar hook-associated protein 2, N-terminal:Flagellar hook-associated 2, C-terminal:Flagellin hook IN, at 4,127,689 to 4,129,140
_3464
Position (kb) 4126
4127
4128 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 4126.237 kb on + strand, within Psyr_3461 at 4126.237 kb on + strand, within Psyr_3461 at 4126.237 kb on + strand, within Psyr_3461 at 4126.237 kb on + strand, within Psyr_3461 at 4126.237 kb on + strand, within Psyr_3461 at 4126.238 kb on - strand, within Psyr_3461 at 4126.238 kb on - strand, within Psyr_3461 at 4126.295 kb on + strand, within Psyr_3461 at 4126.295 kb on + strand, within Psyr_3461 at 4126.295 kb on + strand, within Psyr_3461 at 4126.295 kb on + strand, within Psyr_3461 at 4126.295 kb on + strand, within Psyr_3461 at 4126.295 kb on + strand, within Psyr_3461 at 4126.295 kb on + strand, within Psyr_3461 at 4126.295 kb on + strand, within Psyr_3461 at 4126.295 kb on + strand, within Psyr_3461 at 4126.295 kb on + strand, within Psyr_3461 at 4126.296 kb on - strand, within Psyr_3461 at 4126.296 kb on - strand, within Psyr_3461 at 4126.347 kb on - strand, within Psyr_3461 at 4126.347 kb on - strand, within Psyr_3461 at 4126.467 kb on + strand, within Psyr_3461 at 4126.467 kb on + strand, within Psyr_3461 at 4126.522 kb on + strand, within Psyr_3461 at 4126.522 kb on + strand, within Psyr_3461 at 4126.522 kb on + strand, within Psyr_3461 at 4126.522 kb on + strand, within Psyr_3461 at 4126.522 kb on + strand, within Psyr_3461 at 4126.522 kb on + strand, within Psyr_3461 at 4126.682 kb on + strand at 4126.682 kb on + strand at 4126.742 kb on + strand at 4126.742 kb on + strand at 4126.743 kb on - strand at 4126.743 kb on - strand at 4126.743 kb on - strand at 4126.744 kb on + strand at 4126.744 kb on + strand at 4126.745 kb on - strand at 4126.745 kb on - strand at 4126.769 kb on + strand at 4126.783 kb on + strand at 4126.783 kb on + strand at 4126.783 kb on + strand at 4126.783 kb on + strand at 4126.784 kb on - strand at 4126.784 kb on - strand at 4126.849 kb on + strand at 4126.871 kb on + strand at 4126.871 kb on + strand at 4126.872 kb on - strand at 4126.872 kb on - strand at 4126.873 kb on + strand at 4126.873 kb on + strand at 4126.873 kb on + strand at 4126.873 kb on + strand at 4126.874 kb on - strand at 4126.874 kb on - strand at 4126.964 kb on + strand, within Psyr_3462 at 4126.964 kb on + strand, within Psyr_3462 at 4126.965 kb on - strand, within Psyr_3462 at 4126.975 kb on + strand, within Psyr_3462 at 4127.152 kb on + strand at 4127.177 kb on + strand at 4127.294 kb on + strand, within Psyr_3463 at 4127.294 kb on + strand, within Psyr_3463 at 4127.294 kb on + strand, within Psyr_3463 at 4127.294 kb on + strand, within Psyr_3463 at 4127.294 kb on + strand, within Psyr_3463 at 4127.294 kb on + strand, within Psyr_3463 at 4127.295 kb on - strand, within Psyr_3463 at 4127.295 kb on - strand, within Psyr_3463 at 4127.295 kb on - strand, within Psyr_3463 at 4127.295 kb on - strand, within Psyr_3463 at 4127.295 kb on - strand, within Psyr_3463 at 4127.295 kb on - strand, within Psyr_3463 at 4127.552 kb on - strand at 4127.591 kb on + strand at 4127.591 kb on + strand at 4127.606 kb on + strand at 4127.636 kb on - strand at 4127.672 kb on - strand at 4127.733 kb on + strand at 4127.733 kb on + strand at 4127.733 kb on + strand at 4127.733 kb on + strand at 4127.733 kb on + strand at 4127.734 kb on - strand at 4127.734 kb on - strand at 4127.734 kb on - strand at 4127.734 kb on - strand at 4127.785 kb on - strand at 4127.785 kb on - strand at 4127.786 kb on + strand at 4127.786 kb on + strand at 4127.786 kb on + strand at 4127.786 kb on + strand at 4127.787 kb on - strand at 4127.787 kb on - strand at 4127.787 kb on - strand at 4127.818 kb on - strand at 4127.820 kb on + strand at 4127.820 kb on + strand at 4127.821 kb on - strand at 4127.821 kb on - strand at 4127.868 kb on + strand, within Psyr_3464 at 4127.869 kb on - strand, within Psyr_3464 at 4127.871 kb on + strand, within Psyr_3464 at 4127.872 kb on - strand, within Psyr_3464 at 4127.872 kb on - strand, within Psyr_3464 at 4127.872 kb on - strand, within Psyr_3464 at 4127.914 kb on - strand, within Psyr_3464 at 4128.130 kb on - strand, within Psyr_3464
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 4,126,237 + Psyr_3461 0.70 +3.9 4,126,237 + Psyr_3461 0.70 -1.0 4,126,237 + Psyr_3461 0.70 -0.9 4,126,237 + Psyr_3461 0.70 +3.9 4,126,237 + Psyr_3461 0.70 -0.3 4,126,238 - Psyr_3461 0.70 -0.6 4,126,238 - Psyr_3461 0.70 +1.2 4,126,295 + Psyr_3461 0.74 -1.7 4,126,295 + Psyr_3461 0.74 +1.1 4,126,295 + Psyr_3461 0.74 +3.4 4,126,295 + Psyr_3461 0.74 +1.6 4,126,295 + Psyr_3461 0.74 -0.8 4,126,295 + Psyr_3461 0.74 +0.3 4,126,295 + Psyr_3461 0.74 -2.0 4,126,295 + Psyr_3461 0.74 +0.4 4,126,295 + Psyr_3461 0.74 -0.9 4,126,295 + Psyr_3461 0.74 -2.2 4,126,296 - Psyr_3461 0.74 -0.7 4,126,296 - Psyr_3461 0.74 -0.7 4,126,347 - Psyr_3461 0.78 +2.3 4,126,347 - Psyr_3461 0.78 -0.9 4,126,467 + Psyr_3461 0.86 +0.4 4,126,467 + Psyr_3461 0.86 -2.4 4,126,522 + Psyr_3461 0.89 -1.4 4,126,522 + Psyr_3461 0.89 -3.4 4,126,522 + Psyr_3461 0.89 +0.1 4,126,522 + Psyr_3461 0.89 -0.4 4,126,522 + Psyr_3461 0.89 +1.8 4,126,522 + Psyr_3461 0.89 +4.0 4,126,682 + +4.1 4,126,682 + -2.6 4,126,742 + -0.2 4,126,742 + -1.5 4,126,743 - +1.8 4,126,743 - +1.2 4,126,743 - -1.0 4,126,744 + -1.8 4,126,744 + -1.0 4,126,745 - -1.9 4,126,745 - -1.2 4,126,769 + -0.5 4,126,783 + -2.2 4,126,783 + +1.4 4,126,783 + -4.1 4,126,783 + -3.2 4,126,784 - +0.7 4,126,784 - +3.8 4,126,849 + +1.8 4,126,871 + -2.0 4,126,871 + -3.2 4,126,872 - +2.1 4,126,872 - +2.6 4,126,873 + +0.8 4,126,873 + -1.5 4,126,873 + -1.1 4,126,873 + -2.0 4,126,874 - -2.4 4,126,874 - +1.8 4,126,964 + Psyr_3462 0.37 +1.8 4,126,964 + Psyr_3462 0.37 -0.2 4,126,965 - Psyr_3462 0.37 -1.6 4,126,975 + Psyr_3462 0.40 -1.7 4,127,152 + +0.8 4,127,177 + -0.6 4,127,294 + Psyr_3463 0.30 -0.6 4,127,294 + Psyr_3463 0.30 +2.4 4,127,294 + Psyr_3463 0.30 +1.8 4,127,294 + Psyr_3463 0.30 +1.8 4,127,294 + Psyr_3463 0.30 +1.8 4,127,294 + Psyr_3463 0.30 -0.2 4,127,295 - Psyr_3463 0.31 -2.0 4,127,295 - Psyr_3463 0.31 -2.5 4,127,295 - Psyr_3463 0.31 -1.4 4,127,295 - Psyr_3463 0.31 -4.5 4,127,295 - Psyr_3463 0.31 -0.7 4,127,295 - Psyr_3463 0.31 +1.8 4,127,552 - -0.2 4,127,591 + -0.0 4,127,591 + -0.3 4,127,606 + +2.1 4,127,636 - +2.1 4,127,672 - -1.0 4,127,733 + -3.3 4,127,733 + -1.9 4,127,733 + +2.1 4,127,733 + -0.4 4,127,733 + +2.6 4,127,734 - -2.4 4,127,734 - -0.4 4,127,734 - +2.8 4,127,734 - +1.8 4,127,785 - -0.8 4,127,785 - +3.2 4,127,786 + -1.3 4,127,786 + +1.8 4,127,786 + +1.4 4,127,786 + +2.8 4,127,787 - -1.7 4,127,787 - -0.5 4,127,787 - +1.8 4,127,818 - +3.9 4,127,820 + +2.6 4,127,820 + +4.3 4,127,821 - +3.2 4,127,821 - -1.2 4,127,868 + Psyr_3464 0.12 -2.1 4,127,869 - Psyr_3464 0.12 +3.8 4,127,871 + Psyr_3464 0.13 -1.4 4,127,872 - Psyr_3464 0.13 +1.0 4,127,872 - Psyr_3464 0.13 -0.1 4,127,872 - Psyr_3464 0.13 +2.1 4,127,914 - Psyr_3464 0.15 -1.9 4,128,130 - Psyr_3464 0.30 -1.0
Or see this region's nucleotide sequence