Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3373 and Psyr_3374 are separated by 170 nucleotides Psyr_3374 and Psyr_3375 overlap by 22 nucleotides Psyr_3375 and Psyr_3376 are separated by 19 nucleotides
Psyr_3373: Psyr_3373 - conserved hypothetical protein, at 4,032,166 to 4,032,771
_3373
Psyr_3374: Psyr_3374 - Heavy metal response regulator, at 4,032,942 to 4,033,631
_3374
Psyr_3375: Psyr_3375 - Heavy metal sensor kinase, at 4,033,610 to 4,034,998
_3375
Psyr_3376: Psyr_3376 - lyase, putative, at 4,035,018 to 4,035,686
_3376
Position (kb)
4033
4034
4035 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 4032.750 kb on + strand at 4032.751 kb on - strand at 4032.751 kb on - strand at 4032.751 kb on - strand at 4032.753 kb on + strand at 4032.753 kb on + strand at 4032.775 kb on + strand at 4032.776 kb on - strand at 4032.829 kb on + strand at 4032.829 kb on + strand at 4032.829 kb on + strand at 4032.829 kb on + strand at 4032.830 kb on - strand at 4032.830 kb on - strand at 4032.830 kb on - strand at 4032.888 kb on - strand at 4032.947 kb on + strand at 4032.955 kb on + strand at 4032.956 kb on - strand at 4032.956 kb on - strand at 4033.230 kb on + strand, within Psyr_3374 at 4033.301 kb on - strand, within Psyr_3374 at 4033.442 kb on - strand, within Psyr_3374 at 4033.442 kb on - strand, within Psyr_3374 at 4033.442 kb on - strand, within Psyr_3374 at 4033.442 kb on - strand, within Psyr_3374 at 4033.590 kb on + strand at 4033.590 kb on + strand at 4033.591 kb on - strand at 4033.694 kb on + strand at 4033.695 kb on - strand at 4033.695 kb on - strand at 4033.700 kb on + strand at 4034.110 kb on + strand, within Psyr_3375 at 4034.110 kb on + strand, within Psyr_3375 at 4034.110 kb on + strand, within Psyr_3375 at 4034.110 kb on + strand, within Psyr_3375 at 4034.110 kb on + strand, within Psyr_3375 at 4034.111 kb on - strand, within Psyr_3375 at 4034.111 kb on - strand, within Psyr_3375 at 4034.111 kb on - strand, within Psyr_3375 at 4034.114 kb on + strand, within Psyr_3375 at 4034.114 kb on + strand, within Psyr_3375 at 4034.114 kb on + strand, within Psyr_3375 at 4034.114 kb on + strand, within Psyr_3375 at 4034.114 kb on + strand, within Psyr_3375 at 4034.114 kb on + strand, within Psyr_3375 at 4034.114 kb on + strand, within Psyr_3375 at 4034.114 kb on + strand, within Psyr_3375 at 4034.114 kb on + strand, within Psyr_3375 at 4034.114 kb on + strand, within Psyr_3375 at 4034.114 kb on + strand, within Psyr_3375 at 4034.114 kb on + strand, within Psyr_3375 at 4034.114 kb on + strand, within Psyr_3375 at 4034.114 kb on + strand, within Psyr_3375 at 4034.114 kb on + strand, within Psyr_3375 at 4034.114 kb on + strand, within Psyr_3375 at 4034.114 kb on + strand, within Psyr_3375 at 4034.115 kb on - strand, within Psyr_3375 at 4034.115 kb on - strand, within Psyr_3375 at 4034.115 kb on - strand, within Psyr_3375 at 4034.115 kb on - strand, within Psyr_3375 at 4034.115 kb on - strand, within Psyr_3375 at 4034.115 kb on - strand, within Psyr_3375 at 4034.115 kb on - strand, within Psyr_3375 at 4034.115 kb on - strand, within Psyr_3375 at 4034.115 kb on - strand, within Psyr_3375 at 4034.115 kb on - strand, within Psyr_3375 at 4034.115 kb on - strand, within Psyr_3375 at 4034.115 kb on - strand, within Psyr_3375 at 4034.115 kb on - strand, within Psyr_3375 at 4034.115 kb on - strand, within Psyr_3375 at 4034.115 kb on - strand, within Psyr_3375 at 4034.115 kb on - strand, within Psyr_3375 at 4034.115 kb on - strand, within Psyr_3375 at 4034.115 kb on - strand, within Psyr_3375 at 4034.115 kb on - strand, within Psyr_3375 at 4034.115 kb on - strand, within Psyr_3375 at 4034.115 kb on - strand, within Psyr_3375 at 4034.115 kb on - strand, within Psyr_3375 at 4034.115 kb on - strand, within Psyr_3375 at 4034.116 kb on + strand, within Psyr_3375 at 4034.116 kb on + strand at 4034.116 kb on + strand, within Psyr_3375 at 4034.117 kb on - strand, within Psyr_3375 at 4034.117 kb on - strand, within Psyr_3375 at 4034.117 kb on - strand, within Psyr_3375 at 4034.118 kb on + strand, within Psyr_3375 at 4034.118 kb on + strand, within Psyr_3375 at 4034.118 kb on + strand, within Psyr_3375 at 4034.118 kb on + strand, within Psyr_3375 at 4034.118 kb on + strand, within Psyr_3375 at 4034.118 kb on + strand, within Psyr_3375 at 4034.118 kb on + strand, within Psyr_3375 at 4034.118 kb on + strand, within Psyr_3375 at 4034.118 kb on + strand, within Psyr_3375 at 4034.118 kb on + strand, within Psyr_3375 at 4034.118 kb on + strand, within Psyr_3375 at 4034.118 kb on + strand, within Psyr_3375 at 4034.118 kb on + strand, within Psyr_3375 at 4034.118 kb on + strand, within Psyr_3375 at 4034.118 kb on + strand, within Psyr_3375 at 4034.119 kb on - strand, within Psyr_3375 at 4034.119 kb on - strand, within Psyr_3375 at 4034.119 kb on - strand, within Psyr_3375 at 4034.119 kb on - strand, within Psyr_3375 at 4034.119 kb on - strand, within Psyr_3375 at 4034.119 kb on - strand, within Psyr_3375 at 4034.119 kb on - strand, within Psyr_3375 at 4034.119 kb on - strand, within Psyr_3375 at 4034.119 kb on - strand, within Psyr_3375 at 4034.119 kb on - strand, within Psyr_3375 at 4034.119 kb on - strand, within Psyr_3375 at 4034.119 kb on - strand, within Psyr_3375 at 4034.119 kb on - strand, within Psyr_3375 at 4034.119 kb on - strand, within Psyr_3375 at 4034.119 kb on - strand, within Psyr_3375 at 4034.119 kb on - strand, within Psyr_3375 at 4034.119 kb on - strand, within Psyr_3375 at 4034.119 kb on - strand, within Psyr_3375 at 4034.119 kb on - strand, within Psyr_3375 at 4034.119 kb on - strand, within Psyr_3375 at 4034.119 kb on - strand, within Psyr_3375 at 4034.119 kb on - strand, within Psyr_3375 at 4034.119 kb on - strand, within Psyr_3375 at 4034.119 kb on - strand, within Psyr_3375 at 4034.119 kb on - strand, within Psyr_3375 at 4034.119 kb on - strand, within Psyr_3375 at 4034.442 kb on - strand, within Psyr_3375 at 4034.563 kb on + strand, within Psyr_3375 at 4034.718 kb on - strand, within Psyr_3375 at 4035.052 kb on + strand at 4035.053 kb on - strand at 4035.053 kb on - strand at 4035.053 kb on - strand at 4035.103 kb on + strand, within Psyr_3376 at 4035.103 kb on + strand, within Psyr_3376 at 4035.121 kb on + strand, within Psyr_3376 at 4035.121 kb on + strand, within Psyr_3376 at 4035.220 kb on + strand, within Psyr_3376 at 4035.220 kb on + strand, within Psyr_3376 at 4035.221 kb on - strand, within Psyr_3376 at 4035.221 kb on - strand, within Psyr_3376 at 4035.221 kb on - strand, within Psyr_3376 at 4035.221 kb on - strand, within Psyr_3376 at 4035.221 kb on - strand, within Psyr_3376 at 4035.221 kb on - strand, within Psyr_3376 at 4035.221 kb on - strand, within Psyr_3376 at 4035.334 kb on + strand, within Psyr_3376 at 4035.334 kb on + strand, within Psyr_3376 at 4035.335 kb on - strand, within Psyr_3376 at 4035.335 kb on - strand, within Psyr_3376 at 4035.340 kb on + strand, within Psyr_3376 at 4035.341 kb on - strand, within Psyr_3376 at 4035.341 kb on - strand, within Psyr_3376 at 4035.373 kb on + strand, within Psyr_3376 at 4035.373 kb on + strand, within Psyr_3376 at 4035.374 kb on - strand, within Psyr_3376 at 4035.374 kb on - strand, within Psyr_3376 at 4035.589 kb on + strand, within Psyr_3376 at 4035.589 kb on + strand, within Psyr_3376 at 4035.609 kb on + strand, within Psyr_3376 at 4035.671 kb on + strand at 4035.952 kb on + strand at 4035.952 kb on + strand at 4035.953 kb on - strand at 4035.953 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 4,032,750 + -1.8 4,032,751 - -1.3 4,032,751 - +3.5 4,032,751 - -2.2 4,032,753 + +1.0 4,032,753 + +3.7 4,032,775 + -1.9 4,032,776 - -1.8 4,032,829 + +4.6 4,032,829 + -1.3 4,032,829 + +2.3 4,032,829 + +2.3 4,032,830 - +5.0 4,032,830 - +1.7 4,032,830 - -1.5 4,032,888 - -0.7 4,032,947 + +0.7 4,032,955 + +2.1 4,032,956 - +1.3 4,032,956 - +1.3 4,033,230 + Psyr_3374 0.42 +3.9 4,033,301 - Psyr_3374 0.52 +1.7 4,033,442 - Psyr_3374 0.72 +1.3 4,033,442 - Psyr_3374 0.72 -0.2 4,033,442 - Psyr_3374 0.72 +1.7 4,033,442 - Psyr_3374 0.72 -0.0 4,033,590 + -2.2 4,033,590 + +0.6 4,033,591 - -0.7 4,033,694 + -1.3 4,033,695 - -2.2 4,033,695 - +0.8 4,033,700 + +2.3 4,034,110 + Psyr_3375 0.36 -1.4 4,034,110 + Psyr_3375 0.36 -1.1 4,034,110 + Psyr_3375 0.36 -0.3 4,034,110 + Psyr_3375 0.36 +1.2 4,034,110 + Psyr_3375 0.36 -1.1 4,034,111 - Psyr_3375 0.36 +2.9 4,034,111 - Psyr_3375 0.36 -4.6 4,034,111 - Psyr_3375 0.36 -2.4 4,034,114 + Psyr_3375 0.36 +1.7 4,034,114 + Psyr_3375 0.36 +3.2 4,034,114 + Psyr_3375 0.36 -0.3 4,034,114 + Psyr_3375 0.36 +2.3 4,034,114 + Psyr_3375 0.36 -2.7 4,034,114 + Psyr_3375 0.36 +1.7 4,034,114 + Psyr_3375 0.36 -1.8 4,034,114 + Psyr_3375 0.36 -0.3 4,034,114 + Psyr_3375 0.36 +2.6 4,034,114 + Psyr_3375 0.36 -0.3 4,034,114 + Psyr_3375 0.36 +3.3 4,034,114 + Psyr_3375 0.36 +2.6 4,034,114 + Psyr_3375 0.36 +0.3 4,034,114 + Psyr_3375 0.36 -1.3 4,034,114 + Psyr_3375 0.36 -1.5 4,034,114 + Psyr_3375 0.36 -2.1 4,034,114 + Psyr_3375 0.36 +3.1 4,034,115 - Psyr_3375 0.36 -0.8 4,034,115 - Psyr_3375 0.36 +2.1 4,034,115 - Psyr_3375 0.36 -1.5 4,034,115 - Psyr_3375 0.36 +2.6 4,034,115 - Psyr_3375 0.36 +2.1 4,034,115 - Psyr_3375 0.36 -1.2 4,034,115 - Psyr_3375 0.36 -0.4 4,034,115 - Psyr_3375 0.36 -2.0 4,034,115 - Psyr_3375 0.36 -0.8 4,034,115 - Psyr_3375 0.36 +2.7 4,034,115 - Psyr_3375 0.36 +1.3 4,034,115 - Psyr_3375 0.36 +3.1 4,034,115 - Psyr_3375 0.36 +2.6 4,034,115 - Psyr_3375 0.36 +2.7 4,034,115 - Psyr_3375 0.36 +0.2 4,034,115 - Psyr_3375 0.36 +1.7 4,034,115 - Psyr_3375 0.36 -1.6 4,034,115 - Psyr_3375 0.36 +2.3 4,034,115 - Psyr_3375 0.36 -0.1 4,034,115 - Psyr_3375 0.36 -2.4 4,034,115 - Psyr_3375 0.36 -1.0 4,034,115 - Psyr_3375 0.36 -1.3 4,034,115 - Psyr_3375 0.36 -2.1 4,034,116 + Psyr_3375 0.36 -2.3 4,034,116 + +0.7 4,034,116 + Psyr_3375 0.36 -0.5 4,034,117 - Psyr_3375 0.37 -1.3 4,034,117 - Psyr_3375 0.37 +2.1 4,034,117 - Psyr_3375 0.37 -0.7 4,034,118 + Psyr_3375 0.37 -1.3 4,034,118 + Psyr_3375 0.37 +2.9 4,034,118 + Psyr_3375 0.37 -0.3 4,034,118 + Psyr_3375 0.37 +0.0 4,034,118 + Psyr_3375 0.37 -0.4 4,034,118 + Psyr_3375 0.37 +2.7 4,034,118 + Psyr_3375 0.37 -0.3 4,034,118 + Psyr_3375 0.37 +0.3 4,034,118 + Psyr_3375 0.37 +4.6 4,034,118 + Psyr_3375 0.37 -1.8 4,034,118 + Psyr_3375 0.37 -1.4 4,034,118 + Psyr_3375 0.37 +0.2 4,034,118 + Psyr_3375 0.37 -1.7 4,034,118 + Psyr_3375 0.37 -0.1 4,034,118 + Psyr_3375 0.37 +1.3 4,034,119 - Psyr_3375 0.37 +3.6 4,034,119 - Psyr_3375 0.37 +1.3 4,034,119 - Psyr_3375 0.37 +3.2 4,034,119 - Psyr_3375 0.37 -0.6 4,034,119 - Psyr_3375 0.37 +0.7 4,034,119 - Psyr_3375 0.37 +3.2 4,034,119 - Psyr_3375 0.37 +4.2 4,034,119 - Psyr_3375 0.37 -1.3 4,034,119 - Psyr_3375 0.37 -3.6 4,034,119 - Psyr_3375 0.37 +2.1 4,034,119 - Psyr_3375 0.37 -3.6 4,034,119 - Psyr_3375 0.37 -1.6 4,034,119 - Psyr_3375 0.37 -1.3 4,034,119 - Psyr_3375 0.37 -1.4 4,034,119 - Psyr_3375 0.37 +2.6 4,034,119 - Psyr_3375 0.37 -0.5 4,034,119 - Psyr_3375 0.37 +4.2 4,034,119 - Psyr_3375 0.37 -0.0 4,034,119 - Psyr_3375 0.37 -0.7 4,034,119 - Psyr_3375 0.37 -3.1 4,034,119 - Psyr_3375 0.37 +3.3 4,034,119 - Psyr_3375 0.37 +4.3 4,034,119 - Psyr_3375 0.37 -0.9 4,034,119 - Psyr_3375 0.37 -2.2 4,034,119 - Psyr_3375 0.37 -0.2 4,034,119 - Psyr_3375 0.37 +2.1 4,034,442 - Psyr_3375 0.60 -0.8 4,034,563 + Psyr_3375 0.69 +3.4 4,034,718 - Psyr_3375 0.80 -0.1 4,035,052 + -0.0 4,035,053 - -2.1 4,035,053 - +1.7 4,035,053 - +4.0 4,035,103 + Psyr_3376 0.13 +3.9 4,035,103 + Psyr_3376 0.13 +4.3 4,035,121 + Psyr_3376 0.15 -1.3 4,035,121 + Psyr_3376 0.15 +0.1 4,035,220 + Psyr_3376 0.30 +2.6 4,035,220 + Psyr_3376 0.30 +2.7 4,035,221 - Psyr_3376 0.30 -1.1 4,035,221 - Psyr_3376 0.30 +2.7 4,035,221 - Psyr_3376 0.30 -1.7 4,035,221 - Psyr_3376 0.30 +3.6 4,035,221 - Psyr_3376 0.30 +0.6 4,035,221 - Psyr_3376 0.30 -0.1 4,035,221 - Psyr_3376 0.30 -1.3 4,035,334 + Psyr_3376 0.47 -2.6 4,035,334 + Psyr_3376 0.47 -0.9 4,035,335 - Psyr_3376 0.47 +0.5 4,035,335 - Psyr_3376 0.47 +3.5 4,035,340 + Psyr_3376 0.48 +0.7 4,035,341 - Psyr_3376 0.48 +2.1 4,035,341 - Psyr_3376 0.48 +1.3 4,035,373 + Psyr_3376 0.53 -2.8 4,035,373 + Psyr_3376 0.53 -2.0 4,035,374 - Psyr_3376 0.53 -0.5 4,035,374 - Psyr_3376 0.53 +3.2 4,035,589 + Psyr_3376 0.85 -0.0 4,035,589 + Psyr_3376 0.85 -1.7 4,035,609 + Psyr_3376 0.88 +0.5 4,035,671 + -1.6 4,035,952 + +2.9 4,035,952 + -2.2 4,035,953 - +0.9 4,035,953 - +0.8
Or see this region's nucleotide sequence