Experiment: KB with Acridine orange 62.5 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3340 and Psyr_3341 are separated by 241 nucleotides Psyr_3341 and Psyr_3342 are separated by 196 nucleotides Psyr_3342 and Psyr_3343 are separated by 404 nucleotides
Psyr_3340: Psyr_3340 - conserved hypothetical protein, at 3,995,541 to 3,996,941
_3340
Psyr_3341: Psyr_3341 - hypothetical protein, at 3,997,183 to 3,997,461
_3341
Psyr_3342: Psyr_3342 - conserved hypothetical protein, at 3,997,658 to 3,997,918
_3342
Psyr_3343: Psyr_3343 - GCN5-related N-acetyltransferase, at 3,998,323 to 3,998,820
_3343
Position (kb)
3997
3998 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3996.549 kb on + strand, within Psyr_3340 at 3996.549 kb on + strand, within Psyr_3340 at 3996.549 kb on + strand, within Psyr_3340 at 3996.549 kb on + strand, within Psyr_3340 at 3996.549 kb on + strand, within Psyr_3340 at 3996.550 kb on - strand, within Psyr_3340 at 3996.550 kb on - strand, within Psyr_3340 at 3996.550 kb on - strand, within Psyr_3340 at 3996.550 kb on - strand, within Psyr_3340 at 3996.550 kb on - strand, within Psyr_3340 at 3996.550 kb on - strand, within Psyr_3340 at 3996.550 kb on - strand, within Psyr_3340 at 3996.550 kb on - strand, within Psyr_3340 at 3996.550 kb on - strand, within Psyr_3340 at 3996.550 kb on - strand, within Psyr_3340 at 3996.556 kb on - strand, within Psyr_3340 at 3996.735 kb on + strand, within Psyr_3340 at 3996.735 kb on + strand, within Psyr_3340 at 3996.735 kb on + strand, within Psyr_3340 at 3996.735 kb on + strand, within Psyr_3340 at 3996.735 kb on + strand, within Psyr_3340 at 3996.736 kb on - strand, within Psyr_3340 at 3996.736 kb on - strand, within Psyr_3340 at 3996.736 kb on - strand, within Psyr_3340 at 3996.941 kb on + strand at 3996.941 kb on + strand at 3996.941 kb on + strand at 3996.942 kb on - strand at 3996.967 kb on - strand at 3997.053 kb on + strand at 3997.053 kb on + strand at 3997.053 kb on + strand at 3997.053 kb on + strand at 3997.054 kb on - strand at 3997.054 kb on - strand at 3997.054 kb on - strand at 3997.054 kb on - strand at 3997.054 kb on - strand at 3997.054 kb on - strand at 3997.065 kb on + strand at 3997.066 kb on - strand at 3997.106 kb on + strand at 3997.106 kb on + strand at 3997.107 kb on - strand at 3997.146 kb on + strand at 3997.201 kb on + strand at 3997.202 kb on - strand at 3997.215 kb on + strand, within Psyr_3341 at 3997.250 kb on + strand, within Psyr_3341 at 3997.332 kb on - strand, within Psyr_3341 at 3997.332 kb on - strand, within Psyr_3341 at 3997.334 kb on - strand, within Psyr_3341 at 3997.334 kb on - strand, within Psyr_3341 at 3997.365 kb on + strand, within Psyr_3341 at 3997.366 kb on - strand, within Psyr_3341 at 3997.393 kb on - strand, within Psyr_3341 at 3997.431 kb on + strand, within Psyr_3341 at 3997.622 kb on - strand at 3997.671 kb on + strand at 3997.671 kb on + strand at 3997.672 kb on - strand at 3997.674 kb on + strand at 3997.674 kb on + strand at 3997.675 kb on - strand at 3997.809 kb on - strand, within Psyr_3342 at 3997.809 kb on - strand, within Psyr_3342 at 3997.840 kb on + strand, within Psyr_3342 at 3997.892 kb on + strand, within Psyr_3342 at 3997.924 kb on + strand at 3997.925 kb on - strand at 3997.925 kb on - strand at 3997.932 kb on + strand at 3997.932 kb on + strand at 3997.932 kb on + strand at 3997.932 kb on + strand at 3997.932 kb on + strand at 3997.933 kb on - strand at 3997.940 kb on + strand at 3997.941 kb on - strand at 3997.941 kb on - strand at 3997.941 kb on - strand at 3997.941 kb on - strand at 3997.978 kb on + strand at 3998.038 kb on - strand at 3998.059 kb on + strand at 3998.060 kb on - strand at 3998.060 kb on - strand at 3998.060 kb on - strand at 3998.115 kb on - strand at 3998.128 kb on - strand at 3998.229 kb on + strand at 3998.325 kb on - strand at 3998.334 kb on + strand at 3998.334 kb on + strand at 3998.334 kb on + strand at 3998.334 kb on + strand at 3998.334 kb on + strand at 3998.334 kb on + strand at 3998.334 kb on + strand at 3998.334 kb on + strand at 3998.334 kb on + strand at 3998.335 kb on - strand at 3998.335 kb on - strand at 3998.335 kb on - strand at 3998.335 kb on - strand at 3998.335 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acridine orange 62.5 ug/ml remove 3,996,549 + Psyr_3340 0.72 +0.7 3,996,549 + Psyr_3340 0.72 +0.7 3,996,549 + Psyr_3340 0.72 -0.8 3,996,549 + Psyr_3340 0.72 -1.0 3,996,549 + Psyr_3340 0.72 +2.3 3,996,550 - Psyr_3340 0.72 -0.7 3,996,550 - Psyr_3340 0.72 -0.6 3,996,550 - Psyr_3340 0.72 +0.1 3,996,550 - Psyr_3340 0.72 -0.3 3,996,550 - Psyr_3340 0.72 -1.8 3,996,550 - Psyr_3340 0.72 +0.7 3,996,550 - Psyr_3340 0.72 +2.6 3,996,550 - Psyr_3340 0.72 +0.7 3,996,550 - Psyr_3340 0.72 +3.7 3,996,550 - Psyr_3340 0.72 +3.1 3,996,556 - Psyr_3340 0.72 +3.7 3,996,735 + Psyr_3340 0.85 +3.4 3,996,735 + Psyr_3340 0.85 +0.7 3,996,735 + Psyr_3340 0.85 -4.0 3,996,735 + Psyr_3340 0.85 +2.9 3,996,735 + Psyr_3340 0.85 +0.9 3,996,736 - Psyr_3340 0.85 +1.3 3,996,736 - Psyr_3340 0.85 -1.4 3,996,736 - Psyr_3340 0.85 -3.3 3,996,941 + +0.7 3,996,941 + +3.2 3,996,941 + +0.4 3,996,942 - +2.9 3,996,967 - -0.3 3,997,053 + -0.8 3,997,053 + +1.3 3,997,053 + -2.2 3,997,053 + -4.6 3,997,054 - -0.8 3,997,054 - -2.5 3,997,054 - +2.9 3,997,054 - -1.8 3,997,054 - +3.4 3,997,054 - -2.4 3,997,065 + -1.0 3,997,066 - -1.8 3,997,106 + -1.7 3,997,106 + +0.3 3,997,107 - -2.3 3,997,146 + -1.4 3,997,201 + -1.7 3,997,202 - -3.0 3,997,215 + Psyr_3341 0.11 +2.1 3,997,250 + Psyr_3341 0.24 -0.4 3,997,332 - Psyr_3341 0.53 -2.6 3,997,332 - Psyr_3341 0.53 +1.7 3,997,334 - Psyr_3341 0.54 -1.1 3,997,334 - Psyr_3341 0.54 -4.2 3,997,365 + Psyr_3341 0.65 -0.4 3,997,366 - Psyr_3341 0.66 -2.0 3,997,393 - Psyr_3341 0.75 -1.3 3,997,431 + Psyr_3341 0.89 -0.8 3,997,622 - +3.1 3,997,671 + +0.7 3,997,671 + +2.6 3,997,672 - -1.9 3,997,674 + +0.7 3,997,674 + +1.5 3,997,675 - -1.6 3,997,809 - Psyr_3342 0.58 +2.9 3,997,809 - Psyr_3342 0.58 +0.4 3,997,840 + Psyr_3342 0.70 +0.0 3,997,892 + Psyr_3342 0.90 -3.0 3,997,924 + +0.7 3,997,925 - -3.6 3,997,925 - +3.9 3,997,932 + -2.7 3,997,932 + +1.3 3,997,932 + +2.9 3,997,932 + +1.3 3,997,932 + -4.3 3,997,933 - +3.6 3,997,940 + +0.7 3,997,941 - -2.3 3,997,941 - +2.9 3,997,941 - -1.0 3,997,941 - +2.6 3,997,978 + -2.2 3,998,038 - +0.0 3,998,059 + -0.3 3,998,060 - +2.1 3,998,060 - -1.5 3,998,060 - +0.4 3,998,115 - -1.7 3,998,128 - +2.7 3,998,229 + +1.3 3,998,325 - +0.4 3,998,334 + -2.8 3,998,334 + -1.1 3,998,334 + -1.5 3,998,334 + -1.3 3,998,334 + +2.3 3,998,334 + +0.7 3,998,334 + +3.1 3,998,334 + -0.7 3,998,334 + -1.6 3,998,335 - +2.3 3,998,335 - +3.7 3,998,335 - +3.1 3,998,335 - -0.7 3,998,335 - -3.0
Or see this region's nucleotide sequence